Engineering

Volume 5, Issue 10 (October 2013)

ISSN Print: 1947-3931   ISSN Online: 1947-394X

Google-based Impact Factor: 0.75  Citations  

Isolation and Characterization of an L-Amino Acid Oxidase-Producing Marine Bacterium

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ABSTRACT

One marine bacterial strain, R3, has been newly isolated from the intertidal zone of Dinghai sea area. Measurements of a-keto acids and H2O2existing in fermentation supernatant were carried out to show that R3 can produce L-amino acid oxidase (LAAO) with a broad substrate specificity. Physiological and biochemical analysis showed that it can grow great at the conditions with sodium chloride concentration of 1.5%-3%, temperature of 15?C -35?C and pH of 6-7. In addition, molecular identification of 16S rDNA was performed to show that R3 was proximal toPseudoalteromonasspp. with the highest identity of 98.5% toPseudoalteromonasrubra. Therefore, it was designated asPseudoalteromonassp. R3. Further studies are required to arrive at a better understanding of this LAAO and secure an application.

Cite this paper

Yu, Z. , Qiao, H. , Qiu, J. , Xu, P. and Li, P. (2013) Isolation and Characterization of an L-Amino Acid Oxidase-Producing Marine Bacterium. Engineering, 5, 376-380. doi: 10.4236/eng.2013.510B076.

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