Author(s)

Inaudis Cejas, Roberto Méndez, Ariel Villalobos, Felix Palau, Carlos Aragón, Florent Engelmann, Domenico Carputo, Riccardo Aversano, Marcos Edel Martínez, José Carlos Lorenzo

Department of Soil, Plant, Environmental and Animal Production Sciences, University of Naples Federico II, Naples, Italy.
Faculty of Agronomy, Universidad de Ciego de ávila, Ciego de ávila, Cuba.
Institut de Recherche pour le Développement, UMR Diversité Adaptation et Developpement des Plantes, Montpellier, France.
Laboratory for Plant Breeding, Centro de Bioplantas, Universidad de Ciego de ávila, Ciego de ávila, Cuba.

The objective of this work was to evaluate if cryostorage of Phaseolus vulgaris L. seeds induced variations in regenerated plants at the phenotypic and molecular levels. A series of agricultural traits was measured on plants grown from control, non-cryopreserved and cryopreserved seeds, and the genetic stability of plants of the second generation was analysed at selected microsatellite loci. The phenotype of the second generation plants was evaluated as well. No statistically significant phenotypic differences were observed for the parameters measured, neither in the first nor in the second generations. Averaging both treatments, about 76% of the seeds had germinated 10 days after sowing. At harvest we recorded plants with about 73 cm in height, 13 stem internodes, 25 fruits, 103 grains and 4 grains per fruit. One hundred seeds weighted about 26 g. The genetic analyses performed on the second generation plants using six nuclear Simple Sequences Repeats (SSR) markers revealed no changes in microsatellite length between control and cryopreserved samples, implying that there was no effect of seed liquid nitrogen exposure on genome integrity. The phenotypic and molecular results reported here confirm that cryostorage is an efficient and reliable technique to conserve P. vulgaris seeds and regenerate true-to-type plants.

Common Bean; Cryostorage; Genotype Variation; Phenotype Variation

Cejas, I. , Méndez, R. , Villalobos, A. , Palau, F. , Aragón, C. , Engelmann, F. , Carputo, D. , Aversano, R. , Martínez, M. and Lorenzo, J. (2013) Phenotypic and Molecular Characterization of Phaseolus vulgaris Plants from Non-Cryopreserved and Cryopre-served Seeds. American Journal of Plant Sciences, 4, 844-849. doi: 10.4236/ajps.2013.44103.