Effect of Dai-Saiko-To (Da-Chai-Hu-Tang) on LDL-Receptor Gene Expression in Human Hepatoma Cell Line (HepG2) - American Journal of Plant Sciences

AJPS > Vol.4 No.2, February 2013

American Journal of Plant Sciences

Volume 4, Issue 2 (February 2013)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

Effect of Dai-Saiko-To (Da-Chai-Hu-Tang) on LDL-Receptor Gene Expression in Human Hepatoma Cell Line (HepG2) ()

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DOI: 10.4236/ajps.2013.42A058 4,046 Downloads 6,391 Views Citations

Author(s)

Akira Iizuka, Fumihiko Yoshie, Sakae Amagaya, Takaaki Yasuda, Maki Iizuka, Haruyo Yamaguchi, Seiji Nagumo, Kazuo Kondo

Affiliation(s)

Clinical Pharmacy Education Center for Integrative Medicine.
Department of Kampo Pharmacological Sciences.
Department of Medicinal Chemistry, Nihon Pharmaceutical University, Saitama, Japan.
Department of Medicinal Plant Science, Hoshi University Ebara, Tokyo, Japan.
Institute of Environmental Science for Human Life, Ochanomizu University, Tokyo, Japan.

ABSTRACT

We previously reported that Dai-saiko-to (Da-Chai-Hu-Tang), a traditional Japanese kampo medicine, increased LDL receptor mRNA expression in the liver of the hypercholesterolemic rabbits. In this study, we focused on LDL receptor gene expression in a human hepatoma cell line (HepG2) treated with Dai-saiko-to extract and the extracts of eight herbs presented in Dai-saiko-to. Dai-saiko-to extract significantly increased LDL receptor gene and SREBP2 gene expression compared with the control. The extracts of four herbs, Bupleurum root, Pinellia tuber, Scutellaria root and Peony root significantly increased the LDL receptor gene expression. Whereas, Jujube, Immature orange, Ginger and Rhubarb extracts did not change the gene expression. These results suggest that Dai-saiko-to increased the expression of the cholesterol transport gene (LDL receptor) regulated by SREBP2 gene in the human hepatoma cell line. The pharmacological activity of Dai-saiko-to against hypercholesterolemia and atheromatous lesions related for these four herbal components.

KEYWORDS

Kampo; Dai-Saiko-To; LDL Receptor; Gene Expression; HepG2

Share and Cite:

A. Iizuka, F. Yoshie, S. Amagaya, T. Yasuda, M. Iizuka, H. Yamaguchi, S. Nagumo and K. Kondo, "Effect of Dai-Saiko-To (Da-Chai-Hu-Tang) on LDL-Receptor Gene Expression in Human Hepatoma Cell Line (HepG2)," American Journal of Plant Sciences, Vol. 4 No. 2A, 2013, pp. 454-459. doi: 10.4236/ajps.2013.42A058.

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[3]	Progression of conventional hepatic cell culture models to bioengineered HepG2 cells for evaluation of herbal bioactivities
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