Female reproductive system of Amaranthus as the target for Agrobacterium-mediated transformation - Advances in Bioscience and Biotechnology

ABB > Vol.4 No.2, February 2013

Advances in Bioscience and Biotechnology

Volume 4, Issue 2 (February 2013)

ISSN Print: 2156-8456 ISSN Online: 2156-8502

Google-based Impact Factor: 1.18 Citations h5-index & Ranking

Female reproductive system of Amaranthus as the target for Agrobacterium-mediated transformation ()

HTML XML

Download as PDF (Size: 330KB) PP. 188-192

DOI: 10.4236/abb.2013.42027 4,260 Downloads 7,606 Views Citations

Author(s)

Umaiyal Munusamy, Siti Nor Akmar Abdullah, Maheran Abd Aziz, Huzwah Khazaai

Affiliation(s)

Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia.
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia.

ABSTRACT

Agrobacterium-mediated transformation through floral dip and rapid selection process after transgenic event had become a preference as it will overcome the difficulties faced in tissue culturing procedures and lengthy time for screening transformed progenies. Therefore, in this study, three constructs, p5b5 (14,289 bp), p5d9 (15,330 bp) and p5f7 (15,380 bp) in pDRB6b vector which has hygromycin as a selectable marker gene were introduced individually into Agrobacterium tumefaciens strain (AGL1). The cell suspension was applied to Amaranthus inflorescence by drop-by-drop technique and was left to produce seeds (T₁). The T₁ seeds were germinated and grown to produce seedlings under non-sterile condition. Hygromycin selection on seedling cotyledon leaves results in identification of 12 putative transformants, three from p5b5, four from p5d9 and five from p5f7. All positive putative transformants that were selected at the first stage through hygromycin spraying showed positive result in leaf disk hygromycin assay and in a construct specific polymerase chain reaction-based assay. A ~750 bp amplified hygromycin gene was further verified through sequencing. Our results suggest that Amaranthus inflorescences were able to be transformed and the transformed progenies could be verified through a combination of simple and rapid methods .

KEYWORDS

Agrobacterium-Mediated Transformation; Amaranthus; Direct PCR; Female Reproductive System; Floral-Dip; Hygromycin

Share and Cite:

Munusamy, U. , Abdullah, S. , Aziz, M. and Khazaai, H. (2013) Female reproductive system of Amaranthus as the target for Agrobacterium-mediated transformation. Advances in Bioscience and Biotechnology, 4, 188-192. doi: 10.4236/abb.2013.42027.

Cited by

[1]	АМАРАНТ: ОСОБЕННОСТИ КУЛЬТУРЫ, ПРИМЕНЕНИЕ, ПЕРСПЕКТИВЫ ВОЗДЕЛЫВАНИЯ В РОССИИ И СОЗДАНИЯ ТРАНСГЕННЫХ …


[2]	Genomics-Assisted Design of Biotic Stress Resistant Vegetable Amaranths
	Genomic Designing for …, 2022

[3]	The Basic Biological Problems in the Development and Utilization of New Forage Grass
	Chinese Bulletin of Botany, 2022

[4]	新型饲草开发利用的基础生物学问题
	植物学报, 2022

[5]	Achievements in Genetic Engineering of Amaranthus L. Representatives
	2021

[6]	Agrobacterium-mediated Transformation and Gus Gene Expression in Amaranthuscaudatus
	2021

[7]	How to Overcome Recalcitrance? Novel Strategies and Recent Advances in the Genetic Transformation of Grain Amaranth
	2021

[8]	Structural and Functional Genomics of Chenopodium quinoa
	2021

[9]	Agrobacterium rhizogenes-mediated transformation of grain (Amaranthus hypochondriacus) and leafy (A. hybridus) amaranths
	Arévalo, AA Estrada-Luna… - Plant Cell Reports, 2020

[10]	PCR analyses of first generation plants of Amaranthus caudatus L. after “floral-dip” genetic transformation
	2020

[11]	Агробактериальная трансформация эксплантов эпикотилей амаранта багряного Amaranthus cruentus L
	2020

[12]	Agrobacterium-Mediated Transformation of Amaranthus cruentus L. Epicotils
	2020

[13]	PCR analyses of first-generation plants of Amaranthus caudatus L. after «floral-dip» genetic transformation
	2020

[14]	Agrobacterium rhizogenes‑mediated transformation of grain (Amaranthus hypochondriacus) and leafy (A. hybridus) amaranths
	2020

[15]	Агробактериальная трансформация эксплантов эпикотилей амаранта багряного Amaranthus cruentus L.
	2019

[16]	“Floral-dip” transformation of Amaranthus caudatus L. and hybrids A. caudatus× A. paniculatus L.
	2018

[17]	ВВЕДЕНИЕ В КУЛЬТУРУ IN VITRO И РЕГЕНЕРАЦИЯ ПОБЕГОВ ИЗ ЭКСПЛАНТОВ ЭПИКОТИЛЕЙ АМАРАНТА AMARANTHUS CRUENTUS
	2018

[18]	From zero to hero: the past, present and future of grain amaranth breeding
	Theoretical and Applied Genetics, 2018

[19]	Agrobacterium–caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L.
	2018

[20]	Genetics and Breeding of Amaranthus Species
	2018

[21]	Changes in phenotype of transgenic amaranth Amaranthus retroflexus L., overexpressing ARGOS-LIKE gene
	Russian Journal of Genetics, 2017

[22]	Speciation and domestication genomics of Amaranthus spp.
	2017

[23]	Germline Transformation for Crop Improvement
	Molecular Breeding for Sustainable Crop Improvement, 2016

[24]	Breeding of Amaranths
	Amaranthus: A Promising Crop of Future, 2016

[25]	Amaranthus: A Promising Crop of Future
	Springer Singapore, 2016

[26]	Изменение фенотипа трансгенных растений амаранта Amaranthus retroflexus L. с конститутивной экспрессией гена ARGOS-LIKE
	2016

[27]	Амарант: особенности культуры, применение, перспективы возделывания в России и создания трансгенных отечественных сортов
	2015

[28]	Создание трансгенного рапса (Brassica napus L.) c конститутив ной экспрессией гена ARGOS-LIKE Ara bidopsis thaliana методом погружения …
	2015

[29]	СОЗДАНИЕ ТРАНСГЕННОГО РАПСА (BRASSICA NAPUS L.) C КОНСТИТУТИВ НОЙ ЭКСПРЕССИЕЙ ГЕНА ARGOS-LIKE ARA BIDOPSIS THALIANA …
	2015

[30]	Construction of Transgenic Rape (Brassica napus L.) Overexpressing ARGOS-LIKE Gene from Arabidopsis thaliana using the Floral Dip Method
	2015

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies