Biological implications of 2-chlorocyclohexa-2,5-diene-1,4-dione toward ribonuclease A - Advances in Bioscience and Biotechnology

ABB > Vol.4 No.1, January 2013

Advances in Bioscience and Biotechnology

Volume 4, Issue 1 (January 2013)

ISSN Print: 2156-8456 ISSN Online: 2156-8502

Google-based Impact Factor: 1.18 Citations h5-index & Ranking

Biological implications of 2-chlorocyclohexa-2,5-diene-1,4-dione toward ribonuclease A ()

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DOI: 10.4236/abb.2013.41004 3,805 Downloads 6,216 Views Citations

Author(s)

Albert R. Vaughn, Caitlin B. Redman, Sophia M. Kang, Jisook Kim

Affiliation(s)

Department of Chemistry, University of Tennessee at Chattanooga, Chattanooga, USA.

ABSTRACT

2-Chlorocyclohexa-2,5-diene-1,4-dione (CBQ) or 2-chloro1,4-benzquinone is one of the common metabolites of polycyclic aromatic hydrocarbons generated through industrial processes. This report describes the biological effects of CBQ toward ribonuclease A (RNase). We also investigated the inhibition of RNase modifications and the reactivity of CBQ toward selected amino acids. The study was carried out by incubating RNase or amino acids with CBQ in a concentration- and a time-dependent manner at 37°C and pH 7.0. SDS-PAGE results showed oligomerization as well as polymeric aggregation of RNase when incubated with CBQ as early as in 10 min. CBQ-induced RNase modifications were inhibited in the presence of NADH or ascorbic acid. CBQ reactivity toward selected amino acids was also evaluated by determining the second-order rate constants for the reactions of CBQ with selected amino acids. It was found that the reactivity toward CBQ decreased in the order of lysine > threonine > serine >> aspartate > cysteine.

KEYWORDS

Chlorobenzoquinone; PAH; Protein Modification; Ribonuclease A

Share and Cite:

Vaughn, A. , Redman, C. , Kang, S. and Kim, J. (2013) Biological implications of 2-chlorocyclohexa-2,5-diene-1,4-dione toward ribonuclease A. Advances in Bioscience and Biotechnology, 4, 22-28. doi: 10.4236/abb.2013.41004.

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