Cosmetic Applications of Regenerative Medicine—A New Approach to the Reduction of Neck Lines

Combining different areas of regenerative medicine is considered the new standard for comprehensive tissue and organ regeneration. This approach can be further applied to cosmetic issues such as deep neck lines. This case report utilized techniques of autologous stem cell therapy and epigenetic tissue en-vironment modulation with externally applied polyphenols and tissue penetration agents. The results showed a remarkable cosmetic improvement with a significant reduction of neck lines in a patient where employment and psychological issues were developing from the original problem. The regenerative medicine protocol was safe, efficient in disposable materials, portable benchtop equipment and surgical operating time. The patient had an excellent outcome for this difficult to treat cosmetic problem.


Introduction
Regenerative medicine is a rapidly developing of medicine incorporating diverse but integrated fields of stem cells, bioscaffolds, biomarkers, molecular epigenetics and DNA editing. Substantial research on regenerative medicine has focused on replacing diseased tissues and organs such as heart, liver, kidneys and pancreas [1]. The use of regenerative medicine technology for treating cosmetic problems is gaining popularity in many parts of Asia and western countries. China, Japan and South Korea have large percentages of the general population with disposable incomes for spending on high quality health Other applications show a crossover of medical and cosmetic medicine such as burns victims and with difficult decision-making placed on immediate relatives [2]. The regenerative approach to treat the skin of burn victims is most important for improved outcomes on both the psychological and physical functional dimensions of afflicted patients. Developing in parallel to these treatments is the cosmetic field, particularly on skin regeneration to obtain a more youthful appearance. Facial and neck aesthetics have major importance in a large percentage of the population, especially women, but with an increasing awareness and utilization of cosmetic products by men. This can be observed in the time taken by people to apply makeup and style hair. Looking young has important positive psychological benefits that translate into better physical and emotional health, and better employment prospects in many industries. In contrast, there can be significant psychological comorbidities in people with facial conditions requesting regenerative treatments [3].

Case Presentation
A 37-year-old Chinese female in good health sought treatment for extensive and multiple neck lines. Her occupation was a professional singer and the appearance of neck lines was affecting her psychologically and employment prospects.
Photos of the neck only were taken to maintain anonymity and were recorded at baseline, and postoperatively at 2 days, 5 days and 5 weeks. The subject gave permission for the images to be used for research publication purposes.
The stem cell therapy used a variation of the technique previously published by the author using stem cells for facial pain and inflammation [5]. In addition, a cosmetic biochemistry approach was included. This consisted of daily self administered applications of a highly absorbable topical serum with a novel formulation of bioactive compounds with stem cell signaling properties. The neck lines were injected with local anaesthetic in small 0.1 ml volume increments. The SVF containing stem cells was injected intradermally along the neck lines with a 25 gauge needle. The subject was given the topical serum formulation and instructed to apply to the neck lines twice daily for 4 weeks.
The subject reported no adverse effects or complications from the procedure.
There was minor visible redness and swelling of the neck lines at the injections sites for 2 days. There was minimal discomfort and no analgesics were taken by the subject. There was rapid and visible improvement of the necklines at 5 days and the reduction of neck lines continued for 5 weeks. The subject was very satisfied with the result (Figures 1-4).

Discussion
Consensus among scientists is the using of multimodal regenerative techniques applicable to the target tissue or organ. Specific for this case of skin neck lines was the administration of stem cells directly to the target site to precisely locate the stem cells. This is a straightforward and simple medical approach using 23 -Open Journal of Regenerative Medicine

Conclusions
This case report demonstrates the approach of integrating different areas of the regenerative medicine field to achieve a significant improvement on skin aesthetics [9]. In contrast, our described surgical protocol was simple, safe and effective.
Importantly, the new technique maintained the patient's privacy of having a cosmetic procedure. Postoperatively, it was not obvious to other work related Open Journal of Regenerative Medicine people and friends. The topical serum formulation was developed by using a novel combination of bioactive compounds with enhanced skin penetration chemistry. The described regenerative medicine methodology is applicable for further applications of cosmetic medicine.