Detection of Human Pharmaceuticals in the Surface Water of East Fork Stones River

Pharmaceuticals have been detected nationwide in different environmental matrices including wastewater effluents and surface water. In recent studies, pharmaceuticals have also been found in aquatic plants, fish tissues and plasma of shark bulls. Pharmaceuticals that were detected as indicated in published literature, included steroids, prescription drugs such as antibiotics, anti-depressants, anti-inflammatory drugs, hormones and over the counter (OTC) drugs. We conducted a monitoring study to detect the presence of pharmaceuticals in East fork of the Stones River located in Rutherford County in Middle Tennessee. East Fork Stones River is a six (6) order river that drains a large portion of Rutherford County including Murfreesboro, Tennessee. Grab water samples were collected from Stones River for three seasons: (summer and fall of 2014 and 2015 and winter of 2015 and 2016) each year. Water quality parameters were also determined in situ using Eureka Water Probes TM multi-parameter sondes. Water samples were analyzed for the presence of pharmaceutical compounds using GC-MS. Chemical Abstract Service Registry Numbers (CASRN or CAS) for detected pharmaceuticals were identified. Pharmaceuticals detected included those used for treatment of chronic alcoholism (Disulfiram: CAS # 97-77-8), a compound (Thiazoli-dine: CAS # 504-78-9) in the drug Thiazolidinedione which is used for the treatment of type II diabetes, a compound associated with the prevention of anti-inflammatory conditions (Methyl palmitate: CAS # 112-39-0), and emollient in skincare (Undecane: CAS # 1120-21-4). While the quantitative concentrations of these drugs were not determined in this study, their qualitative presence in surface water is noteworthy.


Introduction
In the last few decades concerns have been raised about the presence, fate, and impacts of emerging contaminants that are being released in the environment.
Emerging contaminants (ECs) are organic compounds manufactured synthetically and could be found in human health and hygiene products. ECs comprise a vast number of chemicals which include pharmaceuticals, personal care products, endocrine disrupting compounds, pesticides, surfactants, and industrial additives. Most of these chemicals are considered to be newly synthesized compounds and are not yet regulated. Production, consumption and subsequent release of these chemicals in significant volumes in our environment have resulted in the pollution of our ecosystem [1]. There has been an increasing awareness and emphasis among the scientific community to investigate the occurrence, and concentration of the emerging contaminants in the environment [2] [3] [4]. It is due to the growing concerns regarding the presence of these chemicals in the ecosystem especially on non-target organisms [4] [5]. Some of the risks include development of microbial resistance to antibiotics, feminization or masculinization of exposed aquatic organisms, interference with endocrine system of higher organisms, and accumulation in soil, plants, and animals [1] [4] [6] [7] [8] [9] [10]. Pharmaceuticals were recognized as emerging contaminants about two decades ago by Daughton and Ternes [11] [12]. Since then studies have been conducted regarding the occurrence and fate of these contaminants on a global scale employing increasingly sensitive analytical techniques in different environmental matrices. The studies include those done in surface water, groundwater, drinking water, tap water, and wastewater effluents [12] [13] [14]. Pharmaceuticals are chemicals used for diagnosis, treatment (cure/mitigation), alteration or prevention of disease, improvement of health condition or structure/function of the body which include both prescription and over the counter drugs [11]. Despite the research efforts made by the scientific community, we still lack complete understanding of the fate and bioaccumulation of these chemicals in the environment [15]. Pharmaceuticals are one of the most commonly detected chemicals under emerging contaminants [16] [17] [18] [19] [20].
In particular, the compounds that are being addressed include prescription drugs, over the counter drugs, and antibiotics. There are several pathways by which these chemicals can enter and persist in the environment. Activities such as flushing unused or expired prescriptions or over the counter drugs; the improper disposal of drugs by hospitals and manufacturers may also be the conduits for these chemicals into the environment [ [27]. Moreover, US wastewater treatment facilities still lack the infrastructure to analyze treated water for the presence of these contaminants. Most wastewater treatment plants use activated sludge treatment.
However some studies have provided alternative methods to improve removal efficiency of wastewater in treatment plants, especially organic chemicals [28] [29].
Some of the new suggested treatments include ozonation, reverse osmosis, nanofiltration, and activated carbon filters that can provide removal efficiency of up to 99 percent. However, these aforementioned methods are expensive. They could result in metabolites that can potentially be more harmful than the parent organic chemicals [1] [30] [31]. Additionally, the chemical contaminants in the wastewater have been shown to enter surface water through combined sewer overflow during wet weather conditions. The production and consumption of pharmaceuticals are expected to continue to increase partly due to the increase in population and urbanization. Moreover, increase in population will result in the higher production of existing drugs and the discovery of new drugs. Consequently, it will result in continuous and additional release of these chemicals in the environment [15] [32] [33]. One of the biggest concerns regarding the presence of pharmaceuticals in the ecosystem is the lack of information regarding possible impacts that active ingredients can pose on non-target organisms. Studies have shown that pharmaceuticals have been detected in the concentrations range of part-per-trillion (ng/L) to parts-per-billion (µg/L) in both sewage treatment plant effluents and aquatic environments [4] [18] [19]. The presence of trace levels of pharmaceuticals in aquatic environment has been alluded to in several worldwide studies where these chemicals have been detected in fish tissues, plasma of bull sharks and plants [34]- [42]. Moreover, several publications published in the last 7 years reported that organic contaminants may have entered the food chain when they transfer from treated wastewater into crop plants [22] [48]. Studies conducted under real-world conditions are rare [22] [47]. There is a dire need to conduct more research to fill the knowledge gaps regarding potential long-term effects of these chemicals in Our study is based on a watershed scale approach. This approach has been considered as the best framework to study water resource challenges because it is locally focused and it includes all local stressors and stakeholders. Therefore the objectives of the study are as delineated below: 1) To determine the temporal prevalence of pharmaceuticals in surface water of East Fork Stones River.
2) To determine water quality parameters that could affect the quality of water in the stream. It is hypothesized that 1) the presence of pharmaceuticals in surface water contributes to the impairment of surface water quality; 2) that incidence of pharmaceuticals in the streams will fluctuate as a function of the seasons.

Study Sites
For this study, an urbanizing watershed from Middle Tennessee was chosen. The watershed was selected because of the associated land use and its proximity to the main campus of the university. The watershed selected was Stones River watershed (located in Rutherford County).

East Fork Stones River
East Fork Stones River ( Figure 1) is a major stream in Rutherford County. According to the US Census, Rutherford County is the fifth-most populous county in Tennessee, with population of 395,000 and growing. East Fork Stones River is considered a six-order river that drains Middle Tennessee sub-watersheds. The watershed is approximately 921 square miles and drains parts of Cannon, Davidson, Rutherford and Wilson Counties. While it is considered an urbanizing watershed; land use in the county is agricultural and residential. The watershed encompasses agricultural fields of hay and pasture, some row crops, forests, and urban and industrial development. The portion of the East Fork Stones River in the study is in Murfreesboro, TN.

Analytical Methods
Sampling of the pharmaceuticals and physico-chemical water quality parameters was conducted for three seasons for two years: summer (2014)   In-situ monitoring was conducted for water quality parameters of interest.
These parameters were temperature, pH, dissolved oxygen, and turbidity. Measurements of the parameters were taken with Eureka Manta2 TM sonde or data loggers (Eureka Water Probes, Austin TX). The data logger was interfaced with the applicable sensors, as shown in Figure 4, and deployed in the streams to at least a 45-cm depth for about 3 minutes. Prior to deployment, the Manta data logger was calibrated according to instrument specifications. A minimum of two readings were taken in situ, and their average was used for further analysis. Additionally, the discharge rate of the sampling locations during the sampling period was obtained from the United States Geological Survey (USGS) real time database. Figure 5 and Figure 6 represented the flow rates of the East Fork Stones River during the sampling periods.

Analysis of Water Samples for the Presence of Pharmaceuticals
It is not uncommon that pharmaceuticals used by consumers often end up in the   Spectrometry is a wide-ranging analytical technique, which involves the production and subsequent separation and identification of charged species according to their mass to charge (m/z) ratio.

Results and Discussion
The results consist of a two-year (2014-2016) monitoring data of pharmaceuticals in the East Fork Stones River as well as relevant water quality parameters of Journal of Water Resource and Protection interest. As previously indicated, the water quality parameters of interest were temperature, pH, dissolved oxygen, and turbidity.

Temporal Occurrence of Pharmaceuticals in East Fork Stones River
Pharmaceuticals detected in East Fork Stones River are presented here-in according to the seasons. The results are discussed based on the following subdivisions: 1) Pharmaceuticals detected in the East Fork Stones River during summer of 2014 and 2015 (Table 1); 2) Pharmaceuticals detected during Fall of 2014 and 2015 (Table 2); 3) Pharmaceuticals detected during Winter of 2015 and 2016 ( Table 3). The Pharmaceuticals detected during the summer of 2014 and 2015 (Table 1) included Methyl Palmitate, an anti-inflammatory and anti-fibrotic agent; Thiazolidine, a compound in the drug Thiazolidinedione (pioglitazone) which is used for the treatment of type II diabetes; and Undecane, commonly used as an intensive skin care lotion for people with eczema and psoriasis. Additionally, Disulfiram, a chronic alcohol treatment drug was detected during summer of 2014. On the other hand, Eicosane was detected during summer of 2015. Eicosane is an emollient in skin lotions and ointments and can be used for treatment of eczema and psoriasis. Table 2 depicts the pharmaceuticals detected in the fall of 2014 and 2015. The chemicals detected in the fall of 2014 included DEET which is an insect repellent and is used as an insecticide. Another chemical was Eicosane, an emollient used as a skincare ointment, mostly used by patients suffering from eczema and psoriasis. Other pharmaceuticals detected during both fall of 2014 and 2015 included Methyl palmitate, an anti-inflammatory and anti-fibrotic agent; Thiazolidine, a compound in the drug Thiazolidinedione (pioglitazone) which is used for the treatment of type II diabetes; and Thiram, a fungicide or industrial insecticide was detected as well. Trimethoprim which is an antibiotic drug was Journal of Water Resource and Protection    Similarly, the pharmaceuticals that were detected in the winter of 2015 and 2016 are presented in Table 3.

Water Quality of the East Fork Stones River
The motivation for selecting the parameters of interest was based on the importance of these parameters (temperature, pH, turbidity, dissolved oxygen) in water quality. Therefore, in order to assess the water quality of the East Fork Stones River; in-situ monitoring of the water quality parameters was conducted and the findings are being reported below. Duplicate readings were taken at each location and used for further analysis.

River Temperature
Temperature tends to be the most commonly analyzed water quality parameter because it affects both the chemical and biological characteristics of the water.
Moreover, every organism exhibits a preferred range or tolerance for temperature. Therefore, temperature affects the ecosystem functions of a watershed. The temperature results are presented in Figure 8. As expected East Fork Stones River was warmer in the summer months. The temperature values ranged from 7˚C to 27˚C. The accuracy of the temperature sensor is ±0.1 of the reading.
There was no significant difference between the temperature of the upstream and downstream locations of the River as well as between sampling years (2014-15 and 2015-16).

River pH
pH is a measure of acidity or alkalinity over a pH range of 0 -14 in the pH scale.
Water pH is a valuable indicator of chemical water quality because pH can influence the availability of chemicals in the water. Moreover, pH also affects solubility and biological availability of nutrients and heavy metals. As a result, it affects the mobility of many pollutants in a water body. Journal of Water Resource and Protection As shown in Figure 9, the pH of the River was between 7.4 to 8.5. The accuracy of the electrode is ±0.  Figure 9, an increase in pH was observed during these sampling rotations. Even though, pH tends to be higher during the rainy period and snowstorm events. The pH is still on the higher side of expected range. This could be due to the terrain around the river and the farming practices of nearby farms. Additionally, there was no significant difference between the upstream and downstream locations of the watershed and the sampling years.

River Dissolved Oxygen
Dissolved oxygen is a very important water quality parameter. It is highly dependent on temperature. The solubility of oxygen decreases with an increase in temperature and consequently dissolved oxygen decreases. A minimum of 5 mg/L of dissolved oxygen is required to support aquatic life; however, a reading around 7 mg/L is ideal [50].
On the other hand, the values change significantly from summer to winter. Figure 10 shows the dissolved oxygen observed during sampling period. The dissolved oxygen for Stones River ranged from 7.5 mg/L to 14.5 mg/L with an average of 10 mg/L. The accuracy of the DO sensor is ±0.1 of the reading. These values indicate the overall relatively good health of the river because they remain consistent throughout the sampling period of 2014 to 2016.

River Turbidity
United States Environmental Protection Agency (US-EPA) uses turbidity to measure water clarity and as an indicator for sediment load in streams. Turbidity  is expressed by the amount of light that is scattered by material in the water when light passes through water [51]. Turbidity can be caused by several ecosystem events such as soil erosion, water discharge during urban runoff, eroding stream banks, a large number of bottom feeders, and excessive algal growth [52]. Turbidity is measured in nephelometric turbidity units (NTUs). The accuracy of the turbidity electrode is ±2% of the reading. The turbidity for East Fork Stones River is depicted in Figure 11. The turbidity was higher during rainfall events

Seasonal Variation in Water Quality of the East Fork Stones River
The seasonal variation in the water quality of Stones River was determined.
Temperature was used as an indicator of seasonal variation. As shown in Figure   12, pH had no correlation with temperature. As explained earlier, it remained close to the average value for most of the sampling time with exceptions during rainfall and snow events. Similarly, turbidity showed no interaction with temperature ( Figure 13) and the pattern was similar to pH. On the other hand, dissolved oxygen showed a significant relationship with temperature during both years. As shown in Figure 14, the dissolved oxygen tends to be lower with increase in temperature and vice-versa. It is evident from regression coefficient (R 2 = 0.77 and 0.52) that there was strong correlation between dissolved oxygen and temperature. It is especially important for the overall health of the watershed. As discussed before runoff, soil erosion, excessive bottom feeders, and harmful algal blooms can trigger higher turbidity values. In general, the increase in turbidity over a period of time can increase the temperature of the River, which in turn can cause lower dissolved oxygen in the water. Since dissolved oxygen is one of the most important water quality parameter to sustain the aquatic life in water, the changes in dissolve oxygen values can have dramatic effects on the biodiversity of the system.

Conclusion
Our study provided the incidence of pharmaceuticals in surface water of an urbanizing watershed in Middle Tennessee. It is important to note that pharmaceuticals were detected throughout the sampling period. However, some of the  pharmaceuticals were detected more often than others. Central to these pharmaceuticals were Thiazolidine a drug associated with a type II diabetes treatment drug Thiazolidinedione (pioglitazone), Methyl palmitate (an anti-inflammatory compound), DEET (an insect repellent), and Undecane (an emollient in ointments used for skin care). It is very likely that occurrence of these pharmaceuticals may be linked to their widespread use by humans. However, the manufacturers are coming up with alternatives to reduce or eliminate the release of these chemicals in the environment. One such example is the Journal of Water Resource and Protection availability of new insect repellents sold over the counter (OTC), which use natural compounds and they are DEET free. However, as mentioned previously, the organic chemicals present in these pharmaceuticals are non-biodegradable.
Therefore, when released into the environment they tend to persist for a long time until they are removed by some emerging technology. It is imperative to educate the public about the presence and potential effects of these chemicals in our environment. It has been alluded to by several studies that improper disposal of these organic chemicals is one of the pathways to the environment [1] [4] [10] [21] [22] [23]. Therefore, ensuring a proper disposal of the organic chemicals such as pharmaceuticals will certainly reduce the drug load in our aquatic environment. At the same time wastewater treatment facilities need to adopt newer technologies that can provide better removal of the organic chemicals.
Future studies can focus on examining the alternative technologies that can efficiently remove the organic chemicals from wastewater. Since, the potential effects of pharmaceuticals in non-target organisms are still not established. Further studies in this area can shed some light on the scope of the problem.