Bredemeyera floribunda Wild Elemental Content and Comparison with Nutritional Recommendation for Adults: Medicinal Plant Used by the Brazilian Rural Population to Treat Fatigue and Exhaustion

Bredemeyera floribunda Wild (Polygalaceae), also known as pau-de-ovo is a medicinal plant used to treat snakebites, as tonic and fortifiers to treat fatigue and exhaustion. The aim of the present study was to document the knowledge of herbal sellers in the preparation of the so-called “B. floribunda fortifier” and to compare the plant root content with the recommended nutritional values and the permitted limit for medicinal plants. The study revealed that, unlike other regions of Brazil, in the Midwest region, the root of the plant is used as food to treat fatigue and exhaustion. The contents in B. floribunda root decreased in the following order: Ca > K > P > Na > Fe > Mg > Si > Mn > Zn > Cu. Daily intake of 30 grams of B. floribunda roots are considered as an excellent source of Cr, Cu, Mn and Se for people 19 to 50 years of age according to the RDA. In and large amounts consumed daily. The Brazilian legislation does not estab-lish a permissible limit of macro- and micro-elements in medicinal plants; thus, the results presented in this study will contribute to the Brazilian public health policy on medicinal plants.


Introduction
The use of traditional medicine and the remedies employed on it has been encouraged by World Health Organization, since their secular use could confirm their efficacy as medicine [1]. In recent years, traditional medicine has drawn attention from both scientists and general population for various reasons, such as the high price of allopathic medicine and the side effects that they could lead, the lack of access to medical clinics and hospitals by the rural population, as well as the realization of the effectiveness of traditional medicine and its treatments [2]. Moreover, the medicinal plants represent over than 50% of all drugs in clinical use in the world [3].
Brazil is considered to be the rich country in terms to plant diversity known to science [4]. Bredemeyera floribunda Wild is a Polygalaceae, popular known as raiz-de-cobra (Portuguese for snakeroot) e pau-de-ovo (Portuguse, Brazil) [5].
Nowadays, the most popular use to B. floribunda is still against snake venom [6], but rural population also uses it to treat amoebic dysentery, rheumatism and as diuretic [7], and as a tonic [5], its use is indicated against dermatitis and insect bites [8]. The fortifying effect is also much sought after by rural population, who uses shaved roots added to water to provide energy and boost immunity.
The effects to treat snake venom of B. floribunda have been supported by the findings of Pereira et al. (1996) that isolated a saponin (Bredemeyroside D) with this effect [9]. The use as diuretic and as hypotensive agent was proved by Bevevino et al. (1994), who linked these results to the presence of triterpenoid saponins [7].
Some macronutrients and micronutrients are nutritionally essential elements, while some heavy metals are relatively harmful. Potassium [10], magnesium [11], iron [12], calcium [13], phosphorous [14], sodium [15], zinc [16] and selenium [17] are important for human health, however, high intake or lack of these elements can be dangerous [10]- [17]. Ingestion of foods with high levels of elements such as chromium, copper and manganese can cause neurological disorders and liver disease [18]. Nickel intake comes from nickel in drinking water, food and polluted environments, which causes a variety of pathological effects [19]. Another element absorbed by humans due to food intake is aluminum, according to studies; the presence of this metal in the body causes neu-Data on macroelements and microelements present in the B. floribunda roots were quantified by ICP OES [28]; however, to date, the nutritional role or toxicity potential of this plant have not been elucidated according safety standards for the consumption of human beings. In addition, we have not found studies in the literature on how to prepare plant root fortifier. Therefore, documentation of medicinal plants used for the treatment and management of human disease by some rural and indigenous communities is important for public health [29]. In fact, the permissible limits of macroelements and microelements for medicinal plants have not yet been set by Brazil and others countries. Although many studies have been conducted towards knowing B. floribunda molecular structure, none has described its mineral content, nor linked its medicinal uses to it. The knowledge on elemental composition of B. floribunda is very important, once these plant is used to treat ailments and as a fortifier. It is important to avoid possible intoxications in acute or chronic consumption due to ingestion of medicinal plants.
The main objective of this study was 1) to report the method of preparation of the so-called "B. floribunda fortifier" and its dosage, 2) to compare the contents of B. floribunda roots detected in [28] with tolerable maximum intake levels established by Dietary Reference Intakes (RDI) for males, females and lactating aged 19 -50 years [30], and 3) to compare the values with those recommended by World Health Organization (WHO) and other countries for medicinal plants.
Macro-and micronutrients requirements vary depending on age and sex [30], as well as, level of physical activity, dietary habits and pregnancy are also important factors [31]. According to studies, breastfeeding women have higher nutritional needs due to their increased physiological body needs to breastfeed the baby [32]. Hence, to the best of our knowledge, this researches is the first to describe the nutritional role of B. floribunda roots, and the preparation of the so-called "B. floribunda fortifier". Here, we investigated aspects of the collection this medicinal plant as well as the modes of preparation and dosage used in folk medicine.

Materials and Methods
Traditional knowledge of herb sellers is acquired through the learning and teaching of older people, usually family members or restricted communities that use plants for disease treatment. Although we use the term "herb sellers", in Brazil they are popularly called "raizeiros" [33] [34], that is, most of these people have little formal education, and have no knowledge about the chemical composition and active principles of plants. Thus, in the following four subsections, an American Journal of Plant Sciences ethnopharmacological survey was conducted in the city of Campo Grande, Brazil in order to document the herb sellers' knowledge about the B. floribunda plant. The last sub-sections describe the criteria for quantification and comparing of results with others ones.

Research Area
The study to evaluate traditional knowledge of the herb sellers about the use and preparation of medicinal plant B. floribunda was carried out in Campo Grande, largest city of Mato Grosso do Sul state, Midwest region of Brazil. Figure 1 shows the map of Brazil with the coordinates of Campo Grande city (Latitude: −20˚26'34.01''S; Longitude: −54˚38'47.00''W). It occupies a total surface area of 8,096.051 km 2 and an urban area of 154.45 km 2 [35]. The estimated population in 2018 in the state is 2,748,023 people. Campo Grande is located near the Brazilian border with Paraguay and Bolivia. It has semi-humid, hot summers, and notably seasonal with a dry winter season from June through September [36]. In addition to local commerce, livestock and family farming are essential practices for the economic sustainability of this city. The City of Campo Grande is divided into 2 districts, called Anhandui and Rochedinho.
Cattle ranching and agriculture has long been the single economic activities of Mato Grosso do Sul. The vegetation around Campo Grande is a tropical savannah called "Cerrado" (a biodiversity tropical savanna with sandy-soil and low-shrub vegetation). The Cerrado is the second largest biome in Brazil and is rich in traditional medicine [37] [38]. In Cerrado, medicinal plants are collected and marketed by herbal sellers and used by indigenous. Studies on medicinal plants from herbal sellers operating in the center of Campo Grande [33] [34], as well as plants used by indigenous people in Mato Grosso do Sul, Brazil, have been conducted in recent years [39]. However, there is no published information on the "fortifying" made from B. Floribunda root in Midwest Brazil.

Selection of Herb Sellers and Data Collection
The present study was conducted following the guidelines of the Code of Brazilian law, this research was registered with the National Genetic Resource Management System and Associated Traditional Knowledge (SisGen, No. A7716EC). Selection of the herb sellers was based on studies published in [33] [34] [39] and preliminary investigation unpublished of the last author, which found that herbal sellers in the city of Campo Grande possess traditional knowledge about the use and availability of B. Floribunda.
Only fifteen herb sellers from the City of Campo Grande were approached through recommendations. Subsequently, detailed interviews for purposes of information collection were carried out with the two herb sellers that agreed to participate in the study and have signed an individual written prior informed consent (certificate of access compliance -administrative act by which the responsible agency declares that access to genetic heritage or associated traditional knowledge complied with the requirements of the Law n˚ 13,123, dated May 20, 2015). For data collection, this manuscript was based on the article published in [29]. Thus, data were collected from the following questions: 1) Which B. Floribunda parts are frequently used? 2) Which diseases are commonly treated with the plant? 3) Which place and climate are considered important during harvesting of plants? 4) What are the methods of preparation and routes of administration of the B. floribunda? Bredemeyera floribunda Willd (Polygalaceae) is a shrub, reaching up to 2 -5 m, with elliptic leaves, flowers with 6.5 -7.5 mm length, cream color to yellow. This plant grows in Venezuela, Peru, Paraguay and Brazil [40]. This plant has a shrub or lianas shrub with cylindrical, glabrescent, purebulous or villous branches with an indentation of sharp hair. The leaves are simple, alternate, petiolate or subseable. The roots of this plant have almost spongy, bitter and foamy bark when shaken with water [41].

Bredemeyera Floribunda Wild
The medicinal use of B. floribunda backs to reports of the botanic George Gardner (1812-1849), who travelled around the country studying the local flora.
In the time, B. floribunda roots rasped down and given in cachaça (cane spirit) were used to treat snakebites [42]. In addition to the paper published in [5], we find in the bibliographic review that only Barbosa Rodrigues (1891) cites the species of the genus Bredemeyera as tonic [43].

Specimen Collection and Plant Identification
Plant specimens containing flowers and roots were collected in a rural region

Sample Preparation by Microwave Digestion
All solvents and reagents were of the highest commercially available purity grade. The details of the digestion procedure have been published earlier [28]. in three successive steps as in [28]. Digestions were performed on a focused microwave system (Speedwave four, Berghof, Eningen, BW, Germany). After digestion step, the samples were analyzed by ICP OES.

Elemental Analysis by ICP-OES Technique
Data on the contents of macroelements (Na, K, Ca, P) and microelements (Fe,

Comparative Study
In this paper, the values of microelements and macroelements detected in the roots of B. floribunda were compared and evaluate to the limit specification of the DRI values: RDA, AI and UL for adult male (19 -50 y), female (19 -50 y) and lactation (19 -50 y).
The DRIs are reference that help to guide health professionals in planning and assessing for the nutrient needs of individuals and groups of people, estimating nutrient intakes, without posing health risks, and for other dietary components to promote health and reduce the risk of chronic disease [30]. Nutritional claims based on FDA, which proposes that foods with 10% -19% of the daily value per portion are "good source of" nutrition, while foods that contain 20% or more of the daily values per portion are considered "excellent sources of" nutrition [44].
However, if a food contains less than 5% of the percent daily value (DV), it is considered low in that nutrient [44].
In addition, the content value of each element in B. floribunda was compared with the values established by WHO and others countries for medicinal plants [45]. Table 1 below shows the data collected about the herbal sellers interviewed. One of the interviewees owns a small farm cultivate their own medicinal plants. It is a local distributor of medicinal herbs to other sellers and homeopathic pharmacies. Table 2 shows the information arranged according to information on the plant part used in the treatment and type of diseases or food, mode of preparation, common route of administration and dosage. According to the herb sellers, the location of the plant in primary production of crops and livestock should be avoided, it is advisable to look for the plant in Cerrados. Climate is not a factor to consider for root collection. The plant leaves are not used as food or to treat diseases. On the other hand, the bark/roots are used to treat diseases such as hypertension, fatigue and exhaustion; however, these parts of the plant are not used as food. What herbal sellers in folk medicine call "fortifying" is mixing the root or bark of the plant with water. According to herb sellers, the root of the plant can be used to treat hypertension, tiredness and exhaustion. In the infusion process it is recommended to use 30 -50 grams of root or bark for each 500 ml of boiling water for tea preparation. On the other hand, the roots can be used in food, which is considered as fortifier, the preparation method is performed as follows: an amount of 15 -30 grams root scraped or crushed, when mixed with 500 ml water, should be well shaken until it becomes a smooth/creamy paste. In our laboratory we made the creamy paste using 7 grams of scraped root plus 120 ml of water (see Figure 4). According to herbal sellers, the root of the plant "fortifying" when used as creamy paste food can be consumed by breastfeeding women, children and adults.

Data on Selection of Herb Sellers and Data Collection
There is no upper limit on daily intake established by age, sex and weight.
However, according to animal studies, at higher doses the extract of the dried roots of B. floribunda Wild leads to bradycardia and death [7].  In Figure 4, there are saponification processes of B. floribunda in water. In fact, according to data published in [28] the extract data of the B. floribunda roots presented an emulsifying potential and can be inserted as a natural ingredient in order to make products healthier.

Data on Comparative Study
In Table 3, values of mineral concentrations determined in B. floribunda roots are presented in mg/30 g of root used in the smooth/creamy paste preparation method ( Table 2). In addition, Table 3 shows the daily intake values stipulated by RDA/AI and calculation of the percentage of each element in relation to RDA/AI, as well as maximum tolerance limit (UL). The concentration of macroelements and microelements in the roots decreases in the order: Ca > K > P > Na and Fe > Mg > Si > Mn > Zn > Cu. The discussion of each element is presented as shown in the sequence of Table 3.
Potassium, K: In Table 3, the content of K observed in roots of the B. floribunda (16.50 ± 0.504 mg/30 g) correspond to 0.48% ± 0.014% of the AI for males (3400 mg/day), 0.634% ± 0.019% for females (2600 mg/day) and 0.589% ± 0.018% of the AI for lactating women [30]. After comparison of the concentration of K in roots of B. Floribunda with those proposed by the AI, it can be concluded that B. floribunda has low K content for males, females and lactating women aged 19 to 50 years old.
The WHO limit for potassium in medicinal plant has not been established yet [45]. In addition, there are yet no established limits for K tolerable upper intake levels (UL). Until date, there have been no reports of toxicity of potassium from consumption in food. In addition, gastrointestinal symptoms were observed in healthy subjects due to ingestion of potassium in supplements [46]. According to FDA, diets containing foods that are a good source of potassium and that are low in sodium may reduce the risk of high blood pressure [47]. American Journal of Plant Sciences Note: ND = Not determined; a Data published in [28]; b Recommended Dietary Allowances (RDA) [30], *The value for AI is used when there are no calculated values for the RDA. **The ULs for magnesium represent intake from a pharmacological agent only and do not include intake from food and water.
Magnesium, Mg: According to data in Table 3, the content of Mg in the root of B. Floribunda was 9.55 ± 0.277 mg/30 g, which correspond to 2.273% ± 0.065% of the RDA for males aged 19 -50 years (420 mg/day), 2.984% ± 0.008% of RDA for females 19 -50 years (320 mg/day), and 3.080% ± 0.08% -2.984% ± 0.008% of the RDA for lactating women (310 -320 mg/day) [30]. From this comparison on the concentration of Mg in roots of B. Floribunda with those proposed by RDA, it can be concluded that B. Floribunda has low Mg content for males, females and lactating women.
The limit set by WHO for Mg in medicinal plant has not been established yet [45]. For all life stage group presented in Table 3, Mg values were lower than UL values for Mg intake for females, males, children and lactating women (350 mg/day) [30]. Therefore, the consumption of one portion of roots dos not con-  [48], ingestion of foods with high magnesium concentration does not pose a health risk to healthy individuals because the kidneys eliminate excessive amounts of urine [49].
Calcium, Ca: In Table 3, the concentration of Calcium in roots of B. Floribunda was 85.317 ± 2.76 mg/30 g, which correspond to 8.531% ± 0.276% of RDA for males, females and lactating women aged 19 -50 years (1000 mg/day) [30]. The value of Ca concentration in B. Floribunda roots compared to those proposed by the RDA showed that this plant has Ca concentration required for the nutrition of males, females and lactating women aged 19 to 50 years old.
For medicinal plant the WHO limits not yet been established for Ca. The permissible limit set by UL for male, female and lactation was 2500 mg/day. After comparison, concentration of Ca in roots of the B. floribunda with those proposed by UL, it is found that the plant has Ca low this limit. Daily and long-term ingestion of foods or supplements containing large amounts of calcium can cause health damage. According studies, people who consume high amounts of calcium might have increased risks of prostate cancer and others diseases [50]. Although there is a dilemma in calcium supplementation, this element plays an essential role in health [51].
Phosphorus, P: The phosphorus concentration found in B. floribunda was 16.398 ± 0.345 mg/30 g, which corresponds to 2.342% ± 0.049% of the RDA (700 mg/day) for males, females and lactating women aged 19 to 50 years old. Considering the RDA (Table 3) for P, the B. floribunda has contents low of P for males, females and lactation. The concentration of P in the root of B. floribunda is lower than the UL limit (4000 mg/day) [30]. Therefore, this concentration of P in the roots of B. Floribunda does not represent a risk of adverse health effects for males, females and lactation. Phosphorus is an essential nutrient for living organisms. It's a key part of ribonucleic acid (RNA) which plays a role in protein synthesis involving mRNA, rRNA and tRNA [52]. However, it is suggested for sick people to adjust their phosphorus intake [53].
Nickel, Ni: There is no established RDA and AI for nickel. In Table 3, the nickel content of B. floribunda root (0.024 ± 0.0006 mg/30 g) is lower than the permitted plant Ni limits (10 mg/Kg) established by WHO [45], as well as lower than the tolerable upper intake level (1.0 mg/day) [30]. Food is a major source of exposure for most people. The Environmental Protection Agency (EPA) estimates that an average adult consumes 170 µg of nickel per day [54]. After comparison, contents in the B. floribunda root with those proposed by the EPA, it is found that plant accumulate Ni above this limit. It is observed that the B. floribunda root has nickel below the value established by UL, but the value of the content above the consumption estimated by EPA. The effects of exposure to any hazardous substance depend on the dose and duration.
Aluminium, Al: The content of Al in roots of B. Floribunda was 7.149 ± 0.234 mg/30 g (Table 3), however, there are no daily intake limits set by the RDA, AI and UL for males, females and lactating women. On the other hand, B. floribunda root content values are close to the oral intake value for humans stipu-American Journal of Plant Sciences lated by some countries which range from 2.5 to 13 mg/day [55]. For medicinal plants the WHO limit not yet been established for Al.
The intake of foods with aluminum is low compared with the amount of aluminium consumed when taking aluminum-containing medication [56]. Daily intakes of aluminium were estimated based on the Food and Drug Administration's (FDA) Total Diet Study dietary exposure model. Thus, estimates of aluminium intakes ranged from 8 -9 and 7 mg/day for adult men and women [57].
After comparing, levels of Al in the roots of B. Floribunda with those proposed by the FDA, it is found this plant accumulates aluminum close to this value. To some extent, exceeding these FDA values over the long term does not mean that there is a serious health hazard. Therefore, the consumption of one portion of root of the B, floribunda does not confer a risk of adverse effects for males, females and lactation woman aged 19 to 50 years old.
Chromium, Cr: The content of Cr observed in root of B. floribunda was 0.081 ± 0.0012 mg/30 g (Table 3), which correspond to 231.42% ± 3.42% of the AI for males aged 19 -59 years (0.035 mg/day), 324.0% ± 4.80% of the AI for females aged 19 -50 years (0.025 mg/day), and 180.0% ± 2.66% of the AI for lactation aged 19 -50 years (0.045 mg/day) [30]. After comparison, concentration of Cr in roots of B. floribunda with those proposed by the AI, it is concluded that roots of this plant are an excellent source off Cr for males, females and lactation woman aged 19 to 50 years old.
The limit has not yet been established by UL of Cr for these populations in study. On the other hand, the concentration of Cr in the B. floribunda is low, when compared with the value established by WHO in medicinal plants (1.3 mg/day) [45]. According to studies conducted in Greece on the concentration of heavy metals and trace elements in soils, waters and vegetables, such factors do not denote health risk [58]. There is little clinical and scientific evidence that chromium III is toxic to humans due to ingestion of food or supplements. Although there not a higher safe intake level for Cr (UL), the Food and Nutrition Board acknowledged a potential for adverse effects of high intakes of supplemental trivalent Cr and advised caution [59].
Cooper, Cu: The content of Cooper (Cu) in Table 3  In Table 3, the content of Cu in root of the B. floribunda is below the values stipulated by UL for this group aged 19 -50 years (10 mg/day). Therefore, the consumption of one portion (30 g) of roots of this plant not represents a risk of adverse health effects.
The WHO limit has not yet been established for Cu in medicinal plants [45]. However, in countries as China and Singapore, the limit for cooper in medicinal plants at 20 and 150 mg/Kg, respectively [60]. The values obtained in our study to 50 years old.
The maximum tolerable intake level (UL) of iron for men, women and lactation was 45 mg/day [30]. Therefore, the result of Table 3 for Fe is below UL values for the study group. The consumption of one portion of Fe in root of B. Floribunda does not confer a risk of adverse effects. There are no data on limitation of the concentration iron in medicinal plants established by WHO [45]. On the other hand, in Egypt it ranges between 261 mg/Kg to 1239 mg/Kg limit is set for iron in medicinal plants [64]. The value in Table 3 for Fe in units mg per Kg (387.07 ± 9.50 mg/Kg) are within of the values stipulated by Egypt. However, sufficient data to define a sage lower limit for toxic iron ingestions are not available.
Iron is pivotal for the formation of oxygen-carrying proteins, and for the enzymes involved in energy production. In fact, one of the most important biological functions of iron in living systems is its role in energetic metabolism, due to its capacity to donate and receive electrons [65].
Manganese, Mn: In the roots B. Floribunda the concentration of Mn was found to be 0.624 ± 0.015 mg/30 g (Table 3). These concentrations correspond to 27.13% ± 0.652% of the AI for male age 19 -50 years (2.3 mg/day), 34.66% ± 0.388% of the AI for females aged 19 -50 years (1.8 mg/day), and 24.0% ± 0.570% of the AI for lactation woman (2.6 mg/day) [30]. From this comparison, values of the concentration of the root B. Floribunda with those proposed by AI, it is concluded that root B. Floribunda is an excellent source of Mn for male, females and lactation women aged 19 to 50 years old. The tolerable upper intake level (UL) at which no risk of adverse health effects to males, female and lactationg women is 11 mg/day. After comparison of the concentration of Mn in the studied plant with those proposed by UL, it is found that the plant accumulated Mn below this limit. In addition, for medicinal plants the WHO limits not yet been established for Mn. However, the permissible limit set by Egypt [66] for medicinal plants (446 -338 mg/Kg) is higher than that found in B. Floribunda roots (20.80 ± 0.50 mg/Kg).
Manganese is essential to normal immune function, blood sugar regulation, cell energy, reproduction, digestion and carbohydrate metabolism, bone growth American Journal of Plant Sciences and helps in defense mechanisms against free radicals [67].
Sodium, Na: The content of Na observed in root of B. floribunda was 5.332 ± 0.192 mg/30 g (Table 3), which correspond to 0.355% ± 0.012% of the AI for males, female and lactating women aged 19 -59 years (1500 mg/day) [30]. After comparison, concentration of Na roots of B. floribunda with those proposed by the AI, it is concluded that roots of this plant have below concentration of Na for males, females and lactation women aged 19 to 50 years old. Table 3 (5.332 ± 0.192 mg/30 g) was below the UL values for consuming Na in male, females and lactating women (2300 mg/day). Therefore, one portion of 30 g of roots not represents a risk of adverse health effects. There are not permissible limit of Na in medicinal plant stipulated by WHO. Foods with low sodium concentrations are nutritionally important, as excessive dietary salt intake is a major health risk worldwide [68].

Sodium level in
Selenium, Se: High selenium concentration was found in the root of B. floribunda (13.767 ± 0.475 mg/30 g). The obtained results showed that the selenium concentration found in the root of the plant is above 25,030.90% ± 863.63% of RDA for the group of people considered in our study (0.055 mg/day), and is higher than the UL limit (0.4 mg/day) [30]. There are not limit of selenium concentration in medicinal plant in Brazil and World Health organization (WHO). However, manifestation of toxicity symptoms will occur with high Cr intakes in supplements. In fact, according studies, Selenium can have toxic effects at high doses [69]. Thus, daily and continuous ingestion of high selenium plants may cause toxicity in humans.
Zinc, Zn: In Table 3, the content of Zn in roots of the B. floribunda (0.2439 ± 0.048 mg/30 g) correspond to 2.21% ± 0.043% of the RDA for males (11 mg/day), 3.048% ± 0.060% for females (8 mg/day) and 2.032% ± 0.040% of the RDA for lactationg women (12 mg/day). After comparing the concentration of Zn in roots of B. Floribunda with those proposed by the RDA [30], it can be concluded that B. Floribunda has low Zn content for males, females and lactating women. The recommended nutrient intakes for zinc differ depending on whether a mow-, moderate-, or high-bioavailability diet is consumed [70]. Acute zinc toxicity from excessive food ingestion is uncommon.
For medicinal plant the WHO limits not yet been established for Zn. The permissible limit set by UL for male, female and lactationg was 40 mg/day. After comparison, concentration of Zn in roots of the B. floribunda with those proposed by UL, it is found that the plant has Zn low this limit.
Zinc is an essential trace metal for human health; its deficiency is associated in various degenerative diseases of the nervous system. Zinc plays important roles as an antioxidant to combat and suppress oxidative stress [71].

Conclusions
In this article, we document the method of preparation and use of medicinal plant B. Floribunda used by the rural population of Midwest Brazil as food to treat fatigue and exhaustion. We hope that the information from this study will For the first time, this study evaluated that a 30 g equivalent portion of B. floribunda scraped root is an excellent source of Cr, Cu, Mn and Se for people 19 to 50 years old. However, the Cu and Fe content are above the established limit for medicinal plants stipulated by other countries. K, Mg, P, Na and Zn contents in the root of the plant are below 5% RDA. In addition, this does not mean that the plant has no nutritional function for humans.
The results of evaluation in study indicate that the concentration of Ni found in the root of the plant is above the permissible limit set by Environmental Protection Agency (EPA) while the concentration of Al is above the Daily intakes Food and Drug Administration's (FDA). The concentration of Se in root of B. Floribunda is above of limit established by UL, and Cr above the permissible limit stipulated by WHO for medicinal plants.
The study revealed that there are no UL established limits for K, Mg, Ni, Al and Cr for male, females and lactation women, and also, there are no values for K, Mg, Ni, Cu, Fe, Al, Na, Se and Zn stipulated by WHO for medicinal plants.
The mineral composition in medicinal plants has an important role to define dosage of secure uses and maximum health benefits. In this way, the use of B. floribunda in this quantity of 30 g is safe for the consumption for some of the determined elements.
The popular use of B. floribunda, to enhance immunity and as a fortifier, to boost energy is supported by the elemental content of the plant, rich in copper, manganese and iron, elements which are linked to cell energy and immune defense.
Although no toxicity could be seen regarding the adult population, B. floribunda use by Brazilian population is far extensive, so, further studies should be performed in order to secure children and pregnant women.
Thus, the comparative data of our studies have relevant information for the use of medicinal plants that can be included in the data on the plant species included in the Renisus (National Relation of Medicinal Plants of Interest to SUS).