Study of Bacteriological and Parasitological Management Quality of Water in the Villages of Senegal

A survey on the parasitological and bacteriological management was conducted in two villages in southeastern Senegal. The objective of this study was to evaluate the water management modalities in the two villages with ethnic differences and possibly detect factors that could cause waterborne diseases. A questionnaire on water management methods was submitted to one hun-dred one women drawn in the villages of Dielmo and Ndiop. Samples of well water and water stored in canaries were subjected to both parasitological and bacteriological analysis. In both villages, there was a difference in water management with respect to household configuration (monogamous, polygamous). The containers used for the drawing and storage of water were the same in their two villages. No helminth eggs were found in the drinking water, but bacterial germs, especially faecal coliforms (an indicator of human contamination) and Enterocoques (an indicator of animal contamination). These waters do not comply with WHO standards. So it is important to disinfect them before consumption although no cases of gastroenteritis have been reported.


Introduction
The microbiological quality of drinking water is a major concern around the tion of drinking water by excreta (feces) of human and animal origin. According to the World Health Organization, more than 80 infectious diseases can be transmitted by water [5]. Among the pathogens responsible for these diseases we can mention bacteria (Salmonella, Shigella), viruses (hepatitis A and hepatitis E) and parasites (Entamoeba histolytica, Giardia lamblia, Crytosporidium vum ...) [2] [6] [7]. For WHO, in 2017, 71% of the world's population (5.3 billion people) used a safe drinking water service; 90% of the world's population (6.8 billion people) had access to at least one basic service. 785 million people did not even have a basic water supply and 144 million of them had to use surface water. Worldwide, 2 billion people use faecally contaminated water points [8]. Diarrhea remains the second leading cause of death, especially among children under 5 years of age [9] [10] [11] [12]. The main factors favoring these diseases are the lack of hygiene, drinking water and sanitation, but also poor management of water resources, which favors the proliferation of bacterial germs. In Senegal, drinking water supply programs have been in existence for more than half a century and the infrastructure is quite well developed. Yet waterborne diseases persist as elsewhere in the ranking of causes of morbidity. Few studies have been conducted in Senegal on the safety of drinking water in rural areas; those conducted in the outlying districts of Dakar reveal that households do not have adequate sanitation facilities: solid and liquid domestic waste is discharged directly onto the ground and latrines are defective, causing coliform pollution of the water table fecal matter [13]. In the present work, we propose to study the management of drinking water in two villages in dry zones with ethnic and hydrographic differences, to detect if possible the factors that can lead to waterborne diseases (diarrhea) and to serve as a basis for supporting a health strategy.

Study Area
The water quality management took place in two villages: Dielmo and Ndiop.

People Selection
Concessions that are part of the survey protocol on the malaria put in place since ten years were selected in two villages.

Water Sample
We have taken Water samples were collected in all 12 wells of both villages with sterile buckets attached to sterile rope. For parasitological analysis, 5 liters of water were collected in the sterile jerrican and 500 ml of water were collected in the sterile glass bottles with screw cap for the bacteriological analysis. In addition, a sample of water was collected and stored 500 ml of water stored in the canaries with selected with sterile big spoon.

Parasitological Analysis of Water
For helminth eggs numeration in wells water, used the protocol of the EPA were (Environment Protection Agency), procedures 388, 389, 390, 391, 392, 393 Helminth eggs (11-25-91). We modified the quantity of water to analyze, we took 5 liters instead of 20 liters. The validation method used for helminth eggs numeration in water has been made by suspending stools (whose quantity of eggs has been determined by the method of Kato) [16]. In 20 ml of water filtered, then making dilutions of this suspension to evaluate the method sensitivity.

Sociodemographic Features
During this study, 101 women have been interrogated (39 at Dielmo and 62 at Ndiop). They were distributed respectively in 30 and 26 concessions averaging   At Dielmo, 73.2% of women are in the monogamous homes for only 28% at Ndiop. Mandingue women (80%) and Serer (73.3%) local ethnic groups were the most often in the monogamous households, unlike immigrant ethnic groups (Peul and Toucouleur, 50%; Wolof, 21.3%); all Bambara women are in the polygamous households. The study put in evidence the different socio-demographic aspects of Dielmo and Ndiop villages. It turns out that there are two major ethnic groups, Serer at Dielmo and Wolof at Ndiop, this is consistent with the results obtained by other authors [14]. The polygamy was more frequent at Ndiop than at Dielmo. The traditions of the local majority ethnic groups seem to be a determining factor: Serer would not have a polygamous tradition unlike Wolof; this could be explained by the fact that a beef must be slaughtered during the wedding ceremony. Breeder being more numerous at Ndiop, men of this village got married more easily. In addition to their household activity, women often have an activity outside of the concession. They are either commercial, farmers or pupils. Wolof women (72.9%), Peul (91.7%), Toucouleur and Socé (50%) are farmers. All commercial women are Wolof but few (2.08% of women of this ethnic group). All pupils women were Serer and represent 3.2% of their ethnic group. At Dielmo, most families who live in the same compound have common vegetable gardens (56.4%) and house pets (61.5%), while at Ndiop 82.3% of families manage individually their vegetable gardens and 56.5% their livestock. It was noted that in both villages, women had at least three children on average, children are considered like a wealth in rural areas since they constitute a potential free labor force also for field work as for breeding women, as manager of the home have their domestic activity in addition, of the parallel activities (trade, agriculture especially) generating of money that allows them to contribute to the home life.

Children Number
The number of children by ethnic group was also variable ( Figure 2). So, Serer women had on average 3.7 ± 0.4 children, Wolof women on average 3.4 ± 0.3, Peul women on average 3.3 ± 0.6, Mandingue women on average 3.8 ± 1.01, Toucouleur women on average 4.5 ± 0.5, Socé women on average 5.5 ± 1.5 and Bambara women, on average 1. It was noted that women in monogamous households have 3.8 ± 0.4 children on average while those of the polygamous households have 4.1 ± 0.5 children respectively for the first wives 3.6 ± 0.5 children, for the second wives, 1.7 ± 0.6 children for the third wives and 2 ± 1.2 children for the fourth wives.

The Illiteracy Rate
The illiteracy rate of women was high, (87.2% at Dielmo; 96.8% at Ndiop). The other women don't pass the primary teaching level. The ethnic group also influenced this distribution because only Serer women (16.2%) and Wolof women

Mode of Water Management
Beyond the well the water and the dish contamination depends on all the chain of management of the clean "water". We interested ourselves therefore to: that draws, when and how; how water was transported; how it was stocked and in short; how the dish herself was stocked. Wells are the only source of provision in water for the two villages; Dielmo accounted nine and Ndiop three.

Mode of Water Drawing
The two studied villages had a very different management of water (Table 1)  communal (32.3% of draw and 32.8% of storage individuals), the draw frequency also varies. At Dielmo 2 to 10 times in the morning and 1 to 5 times in the evening while at Ndiop they make it 1 -3 times the morning and once in the evening.
What can translate the easier access to well. The polygamous households, the chored water concerns generally more the youngest women: 60% of the first wives, 71.4% of second wives, 83.3% of third wives and all fourth women. It was noted that in polygamous households the percentage of women involved in water fetching was higher in the second and third wives. It was noted that in the polygamous households, the percentage of women concerned by the water chore was higher in the second, third and fourth wives. This is because these women were younger and the tradition maintains a wives hierarchy in polygamous homes, they were therefore more concerned by housework than the first wife.  However, the Bambara women cleaned their containers three times a day, others clean them once a day. Whatever the woman ranks in the household, most between them used pans for transporting water from well to the concession. The woman age, her marital statute and the type of household (mono/polygamous), statutes didn't induce significant differences in behavior across samples.

Mode of Water Storage
All families in Ndiop have containers reserved for storage against 84.6% of families in Dielmo. The main way of storage was canaries especially at Dielmo (90% of families against 72.6% at Ndiop). The simultaneous use of canaries and pans was rare: 7.7% for Dielmo and 9.7% for Ndiop. At Ndiop, some families (17.7%) also use jerricans. Containers in which water was stocked were covered with a tray or a plate. The water storage methods vary little according to the ethnic group of women. The majority of women only use these containers to stock water, the woman age doesn't influence this behavior.

Medicines Use
In Dielmo nearly 82% of women admitted to having their children dewormed (of which 38.5% recently) while in Ndiop only 56% of women did (of which 46.8% a long time ago). Products used for the deworming are mainly the Flagyl and the Vermox. Wolof used more the Flagyl and the Serer more the Vermox, other ethnic groups women used the Flagyl as well that the Vermox. The frequent use of flagyl by Wolof and Vermox by Serer could be an indicator of the prevalence of different intestinal parasitosis prevalence in the different villages where these ethnic groups are the majority. Whatever the ethnic group and the village, most women used products coming from the project station. The education plays an important role in this approach to care because all women who have been to school practice the deworming of children against 64.8% of the illiterate women. Most women admitted to having their children disinfected in the previous six months which shows the positive impact of the medical assistance structures available to the population. Whatever the instruction level or the ethnic group, women obtain in majority medicines to the project station. However, it was observed that the same proportion of educated and unqualified can mention the product name used (average 60%).

Parasitological Contamination of Drinking Water
After well water analysis, no well contaminated with helminth eggs was not observed. No helminth eggs have been found in the well water of two villages, what can be explained by a strong percentage of latrine presence that decreased the anarchical defecations, no use of faecal fertilizers in agriculture decrease as well as the soils contamination [17]. These results, although related to helminth eggs were in agreement of those obtained by [18], concerning well water used for watering vegetables. These results are contrary to those obtained by [19] [20] in Nigeria, in Iran [21] and in Tunisia [22] who detected parasites in parasitic samples, mainly Protozoa. This could be explained by the fact that we used the Kato method which is specific to the search for helminth eggs while other authors used standards to search both helminth eggs and cysts of protozoa.

Bacteriological Contamination of Drinking Water
The wells water of two villages was contaminated by thermotolerant coliforms enterocoques and the sulfite-reducing anaerobic spores (Table 2). It is the same with the drinking water stocked in canaries of concessions. The contamination degree was appreciated according to criteria of the World Organization of Health [23]. The analysis showed a contamination of all wells in both villages by thermotolerant coliforms and enterocoques; this contamination degree is too high. The contamination by the sulfito-reducing anaerobic spores was also observed but only in 3 of the 9 wells of Dielmo. Water stocked in canaries was also contaminated, so at Dielmo, all waters of drink were contaminated by coliforms thermo tolerant whereas at Ndiop, 16 of the 19 canaries were contaminated. Enterocoques contaminate 10 canaries at Dielmo against 17 at Ndiop. The sulfi-Journal of Biosciences and Medicines to-reducing anaerobic spores were not present in the water of drink. The water analysis revealed the pollution of all wells by the bacterial agents of fecal origin, notably the fecal coliforms and enterocoques which is consistent with the results obtained at Yaoundé in Cameroon [24], at Yeumbeul in Senegal [13], in Niamey in Niger [25] and comparable to those found in Bolivia [26]; in the Ontario in Canada [27] who found that 93% and 20% respectively of wells were contaminated by the bacterial agents. More recent studies have confirmed the observations made previously, that of the contamination of drinking water (from wells, springs, boreholes, etc.) by microbial agents (total coliforms and fecal coliforms) rendering them unfit for the consumption [28] [29] [30]. The presence of these bacteria is contrary to WHO standards [23] regarding the bacteriological quality of the drinking water. These different bacteria indicate the contamination origin; thus, the fecal coliforms indicate a human contamination origin and the Enterocoques indicate an animal contamination origin. These different contaminations could be explained by the fact that the animals drink around the wells, deposit the dung on the ground or sometimes drag the containers used for the drawing and the water transport in the two villages. However, it was difficult for this study to predict whether this contamination concerns the whole aquifer or if, it was locally induced by the exploitation conditions. These bacterial germs are responsible for gastro-intestinal illnesses [27].

Conclusion
The present study was carried out from August 2001 to April 2002. We noted the predominance of two big ethnic groups: the Wolof (at Ndiop) and the Serer