Monitoring the Treatment of Hepatitis C Viral Infection by Molecular Techniques

The prevalence of Hepatitis C viral infection in Egypt is the highest rate in the world. It is a major public health problem in Egypt. The objective of this study was to detect the clearance of HCV-RNA in Egyptian patients who were recommended for combination therapy of pyglated interferon α 2a and ribavirin (PEG-interferon/Ribavirin). Ninety-five positive HCV-IgG cases were tested for HCV-RNA at baseline and at weeks 4, 12, 24, 48 and 72 of treatment with PEG-interferon α 2a/Ribavirin. The correlations between the viral parameters during and after treatment were evaluated. Patients recommended receiving PEG-interferon 2a/ribavirin combination therapy showed high response rate determined as 71.9% achieved Sustained Virological Response (SVR) from total patients, and 71.4% from patients who received 48 weeks therapy. In general, the employment of TMA provided us the accuracy of results with confidence in our work. There was a significant relation (p < 0.001) between results of Transcription mediated amplification (TMA) and response to therapy that indicate to positive correlation. The development of sensitive accurate assays for HCV-RNA detection and quantification is necessary to improve not only the assessment of the response to antiviral therapy but also our understanding of the mechanism underlying antiviral resistance.


Journal of Biosciences and Medicines
tion is a serious medical problem in Egypt and it has a devastating impact on the Egyptian economy. It is estimated that over 18% of Egyptians are infected by the virus [1] [2]. So Egypt considered as one of the world's highest prevalence of HCV with a majority of genotype-4 infection [3] [4]. Nucleic acid tests (NATs) directly detect the presence of HCV RNA using a combination of amplification and detection techniques. NATs are classifies into qualitative and quantitative tests to detect HCV RNA. In deed the response to the therapy and its successfulness are very important to be detected using more sensitive and specific qualitative and quantitative tests. Transcription mediated amplification (TMA) is the most sensitive qualitative HCV RNA assay, of detection limit that start from 5 IU/ml and sensitivity more than 98% to confirm viremia and to detect clearance of viremia during and after the completion of the treatment [5]. The branched DNA (bDNA) is the first Food and Drug administration (FDA) approved technique to quantify HCV RNA. The method differ from RT-PCR tests, in that the detection signals is amplified rather than target RNA. The third generation assay (bDNA 3.0) has a high dynamic range of lower detection limit of 615 IU/ml and an upper range of 7.7 million IU/ml. It is highly reproducible and the specificity range from 96% to 98% [6]. So the previously mentioned nucleic acid tests (NATs) are very important combined with each other's to monitor clearance of HCV-RNA in therapeutic treated patients with a global TMA technology Confirmation. This study aimed to investigate the clearance of HCV-RNA in Egyptian patients who were recommended for combination therapy of pyglated interferon α 2a and ribavirin (PEG-interferon/Ribavirin). This enables us to evaluate the different responses to the combination therapy of PEG-interferon/Ribavirin as a good used regimen in treatment of chronic HCV in Egyptian patients.

Study Population
This study (approved by the Ethical Committee of Ain Shams University) included 85 HCV-Ab positive cases collected from Cairo, Egypt (El Hussein and Sayed Galal hospitals), Al Menia (El Menia hospital) and Al Daqahlya (Al Mansoura general hospital) from October 2017 to March 2019. Ethics approval was obtained for the study and informed consent forms were signed by patients. The 85 cases included 45 females and 40 males between 18 -65 years of age. The subjects were divided into two groups: First group were positive HCV-Ab and titre of HCV-RNA is less than 615 IU/ml (n = 28) and Second group were positive HCV-Ab and titre of HCV-RNA is more than 615 IU/ml (n = 57). Blood samples were drawn from all study participants and serum samples were separated and stored at −80˚C until further testing.

Study Population Characteristics
Eighty-five Cases were considered newly identified HCV-Ab Positive.14.1% of whom were between 18 -40 years old, 47.1% were between 41 -56 years old, and 35.1% 56 years or more Table 1. According to the results obtained by b-DNA assay based on the baseline value of 615 IU/ml of HCV-RNA, the study population was divided into 2 groups. The first group, representing (33%) of the total, included 28 with positive HCV-Ab and HCV-RNA < 615 IU/ml (11 females and 17 males), whose age range was between 18 -50 years, with a mean age of 36.1 ± 8.2 years. Those patients were defined as viral self-clearers. Accordingly, those patients of first group were also excluded from our study. Since they showed undetectable titre of HCV-RNA < 615 IU/ml by using b-DNA and also they gave negative results with TMA technique (the basis of TMA detection limit 5 IU/ml) ( Figure 1). The Second group, representing (67%) of the total, included 57 with positive HCV-Ab and HCV-RNA > 615 IU/ml (34 females and 23 males), whose age range was between 25 -65 years, with a mean age of 42.03 ± 11.1 years. All cases of group two were recommended for treatment PEG-interferon α 2a therapy (180 ug) for the most majority of the patients and (160 ug) for the minority of the patients, once a week dose combined with ribavirin (1000 to 1200 mg) for all patients daily by their physicians.
All patients of group two were subjected to quantitative assay (b-DNA) and qualitative assay (TMA) according to the following protocol:

Correlation between HCV-RNA Quantitative by b-DNA and Final Sustained Response to Therapy (SVR)
The relation between patients' response to therapy and HCV-RNA quantification value at week 4, 12 was statistically significant (P-value < 0.05). At

Correlation between Qualitative Determination of HCV-RNA by TMA and Sustained Final Response to Therapy (SVR)
The relation between patients' response to therapy and qualitative detection of HCV-RNA by TMA method at weeks 24, 48 and 72 was statistically significant  (Table 3). In positive HCV-RNA patients, the sustained response to therapy could not be reported.

Discussion
Our study proceeded by selection of 85 positive HCV-Ab patients. HCV-RNA quantitative by b-DNA was performed firstly for all positive HCV-Ab patients, followed by TMA qualitative assay. The later technique has applied to confirm viremia more accurately [5] [7]. The results were obtained as <615 IU/ml below the detection limit in 28/85 patients (First group) and >615 IU/ml in the rest majority (57/85) patients (Second group). In the first group, the results were negative for HCV-RNA by TMA. The results on the basis of TMA detection limit 5 IU/ml makes it the most promising tool indicating the clearance of viremia with confidence [3] [8]. The patients of group one, they defined as viral selfclearers, representing 32.95%. This fall in agreement with studies, Seeff (2002) [9], Wong and Lee (2006) [10] and Ponziani et al. [11] reported that 15% -50% clear the infection. The mechanism of clearance of viremia in those patients may be attributed to their immune response against infection and may be explained due a strong multi-specific CD4+ T cell response produced early in infection that is associated with viral clearance and disease resolution, Whereas, a narrowly focused and delayed response is associated with chronic infection [12] [13]. Also, a strong multi specific CD8+ T cell response produced early in infection is associated with viral clearance [14]. In this study, the majority of the patients (57/85) were recommended to receive combination therapy of peglated interferon plus ribavirin (PEG-interferon α 2a/RBV). The current impression that the patients infected with HCV genotype 4 are difficult to treat and that they respond poorly to interferon therapy was made based on earlier studies where conventional interferon-alpha (INF-α) was used alone [15]. On the other hand, studies that used PEG-interferon/RBV on genotype 4 patients showed better results [16]. Those authors recommended treatment for patients with HCV genotype 1 and 4 with PEG-interferon plus RBV for 48 weeks. The data of this study were monitored at baseline and at weeks 4, 12, 24, 48, and 72 of follow up According to the study that carried out by Al Ashgar et al. [16]. In studying of virological response; the viral load was determined quantitatively by b-DNA as the main technique to determine rapid virological reponse (RVR) and early virological response (EVR) at weeks 4 and 12 from therapy respectively. The primary response to the therapy represented by significant decline in viral load [17]. At week 24 all patients who previously achieved EVR (Responders), those patients were recommended to assess the presence of HCV-RNA with a sensitive technique in which the lower detection limit: 50 IU/ml or less at week 24 from therapy [17]. So, TMA qualitative assay was applied as the best detector for HCV-RNA, also it was used as qualitative technique at end of treatment at 48 week, and at week 72 to determine patients achieved end of treatment response (ETR) and sustained virological response (SVR), respectively [3] [18] [19]. In weeks 24, 48 and 72, b-DNA was applied to non-responders for its minor role with non-significant value in treatment intervals of treatment. It just applied to detect the variation in HCV-RNA titers.
In the results of this study, the rapid virological response (RVR) was found in 24/57 patients (42.1%). These results differ from the study by Martin-Carbonero et al. [20] who reported 26.1% RVR in all HCV patients. The patients achieved RVR (24 patients) could achieve SVR (21 patients) by 87.5%. The data of this study similar to that reported by Martin-Carbonero et al. [20] who stated that patients achieved RVR could achieve SVR by 83% for HCV cases.
Early viral response (EVR) in this study was shown in 53/57 patients 93% similarly, Al Ashgar et al. [16] found EVR in 75.3% in their patients. In other studies carried out on the effect of INF plus RBV, other study found that EVR was achieved by 50% and 43% respectively [21]. These revealed the superiority of PEG-interferon/RBV than the conventional interferon during therapy. In this study, the patients achieved EVR (53 patients) could achieve SVR (41 patients) by 76%. Treatment must be continued when there is a 2-log drop in HCV-RNA Journal of Biosciences and Medicines level, or when HCV RNA is undetectable at week 12, in these patients HCV-RNA detected at week 24 by the most sensitive qualitative technique [17].
This study reveals that there were 3 patients recommended to stop therapy at week 24; 2 patients as non-responders and one patient as responder without compromising SVR characterized by low baseline viral load 36.780 IU/ml, undetectable viremia at weeks 4 and 12, and negative HCV RNA by TMA at week 24, indicating the actual clearance of viremia [20].
Sustained virological response (SVR) was achieved in this study by 40/57 patients (71.42%) that vary from results reported by Martin-Carbonero et al. [20] who showed 32.6% SVR, and Wong and Lee [10] summarized results of randomized controlled trials by several authors Lindsay et al. [22] and Derbala et al. [15] which gave also a range of 33% -77% SVR. Also, this result was in agreement with previous studied by Zekri et al. [23] as their patients with genotype 4 showed SVR rates of 68% when treated with PEG-interferon/Ribavirin combination treatment for 48 weeks.