Comparative Analysis on the Haematological Parameters and Antioxidant Levels of Parboiled and Un-Parboiled Dichlorvos (DDVP)-Treated Beans

This study comparatively evaluates the haematological and antioxidant effects of differently prepared (parboiled and un-parboiled) beans treated with sniper (i.e. a dichlorvos insecticide) and fed to wistar albino rats. Thirty (30) male wistar albino rats of known body weight were assigned into six (6) groups of 5 each. The groups received: 70 g and 30 g of un-parboiled beans (groups 1 & 2), 70 g and 30 g (groups 3 & 4) of parboiled beans compounded in the pellets as feed stock, beans only and standard feed for a period of 30 days. The rats were euthanized, and blood samples were collected after the termination of the study. Dichlorvos significantly decreased (P < 0.05) the concentration of the antioxidant enzymes (GSH, GPx, SOD, CAT) in the groups fed with un-parboiled beans unlike the groups fed with parboiled beans which were non-significantly decreased. There was significant increase in the malondialdehyde concentration of the groups fed with un-parboiled beans. Dose dependent variations were seen in the packed cell volume (PCV), white blood cell (WBC), haemoglobin (Hb) and platelet. But a reduced concentration of red blood cell count (RBC) for the un-parboiled groups and an increase in the parboiled group were seen, although both were not significant (P < 0.05).

considered to improve nutrition in food, and its use is assumed an economic, labor-saving, and efficient tool for pest management [1]. They are poisons; they are produced because they are toxic to one pest or the other [2]. Pesticides are an important management tool in agricultural enterprise; they increase yields and increase protection against insects at post-harvest and storage, and it has continued to be the bedrock of agriculture in modern times because of its unquantifiable benefits one of which include enhancement of shelf life of stored agricultural products [3]. Furthermore, pesticide is believed to improve competitive advantage in agriculture [4]. This is because pesticide use is deemed essential for retaining current production and yield levels, as well as maintaining a high-quality standard of life [4]. Organic phosphate is the most widely used pesticide worldwide and their metabolites are wide spread across different populations [5].
In Nigeria, cowpea is known by names such as, "akidi" in Igbo language, "wake" in Hausa language and "ewa" in Yoruba language. It makes a major part of Nigerian food. Vigna unguiculata is an important crop which plays a significant role in the diets of Nigerians serving as a major source of protein in the absence of sufficient animal protein for the population. It is rich in the amino acid lysine and tryptophan, as compared to cereal grains. Cowpeas are consumed either alone, or in combination with cereals to enhance the protein value. It could be cooked, made into soup or milled mixed with pepper, onions, groundnut oil, fried and taken as akara or it is wrapped with leaves and taken as "moin-moin" [6]; [7] but in all these methods of preparation, some people parboil beans while others do not parboil which is also dependent on the type of food they want to make from beans.
Dichlorvos or 2,2-dichlorovinyl dimethyl phosphate (commonly abbreviated as an DDVP) is an organophosphate, widely used as an insecticide to control household pests, in public health, and protecting stored product from insects [8]. It is used for the protection of greenhouse plants, fruits and vegetables against mushroom flies, aphids, spiders, mites, caterpillars and white flies. In Nigeria, DDVP is widely used by farmers for agricultural pest control [9]. The routes of exposure to DDVP include ingestion, inhalation and dermal exposure [10]. It is known that organophosphorus compounds, including DDVP, elicit neurotoxicity in mammals by inhibiting acetylcholinesterase activity [11]. Significant contamination of dried beans has been reported in Nigeria [9]. Beans are among the most versatile and commonly eaten foods throughout the world, and many varieties are grown in the US. Because of their nutritional composition, these economical foods have the potential to improve the diet quality and long-term health of those who consume beans regularly [12].
Oxidative stress has been identified as an important molecular mechanism of toxicity of DDVP [13]. It is defined as an imbalance between the production of free radicals and the antioxidant system of the body [14]. Enzymatic and nonenzymatic antioxidants scavenge free radicals and reactive oxygen species (ROS) in biological systems [15]. Journal of Biosciences and Medicines Several studies have been conducted to evaluate the effect of dichlorvos insecticide on blood and purpose of this study is to comparatively determine whether various preparations of contaminated beans could protect male Wistar rats from the adverse effects of sub-acute dichlorvos toxicity. Also evaluate the toxicity of DDVP with common local beans preparation on oxidative stress which has been identified as one of the important molecular mechanisms of DDVP toxicity. DDVP toxicity should be explored because the residues of DDVP have been detected in food stuffs in several countries including Nigeria [16].

1) Beans preparation
Beans were bought in Ultra-modern market, Ubani, Umuahia, Abia State, Nigeria. dichlorvos based-pesticide, Sniper, was mixed with beans to achieve 1 ml/kg and air dried for one (1) week. The contaminated beans were washed and prepared (cooked) separately where one group was parboiled for five (5 mins) minutes, the water was removed and a fresh water was added and cooked without the addition of any spices while the other group was completely cooked without parboiling.

2) Feed formation
The parboiled and un-parboiled beans were sundried and grounded into flour, mixed with standard feed stock at different ratios for the study. The samples were all pelletized for easy consumption by the rats.

Chemicals
Commercial grade dichlorvos (DDVP, 100% solution containing 100 g per liter of 2,3-dichlorovinyldimethylphosphate) marketed as Sniper® was purchased from an agrochemical company in Umuahia, Nigeria. DDVP was reconstituted in soya oil (SO, Grand Cereals and Oil Mills Limited, Jos, Nigeria) to a 5% stock solution.

Experimental Animals
The male Wistar rats used for this study were obtained from Department of Zo-
At the end of the experimental weeks, the animals were euthanized and blood samples collected through cardiac puncture using 2 ml syringes. Blood samples for hematological parameters were collected in EDTA-containing sample bottles and used for the tests.

Determination of MDA Concentration
The concentration of malondialdehyde (MDA) was evaluated in the serum. The method described by Wallin et al. (1993) [17] was used. The principle of the method was based on the spectrophotometric measurement of the colour developed during the reaction of thiobarbituric acid with MDA. The solutions were cooled under tap water and the absorbance was measured with an ultraviolet (UV) spectrophotometer (T80 + UV/Visible Spectrophotometer, PG Instruments Ltd., Leicestershire, United Kingdom) at 532 nm. The concentration of MDA in the samples was calculated by using the absorbance coefficient, MDA-TBA complex 1.56 × 10 5 cm −1 •M −1 .

Assays of the Activities of Serum Antioxidant Enzymes
Superoxide dismutase activity was assayed by the method of Arthur and Boyne [18] as contained in Randox kit. Catalase activity was assayed with the randox kit according to the method described by Sinha [19]. Glutathione peroxidase (GPx) was measured according to the method of Paglia and Valentine [20], while the reduced glutathione level was determined by the method of Exner et al.

Hematological Evaluations
The total white blood cell count was determined by haemocytometry following

Data Analysis and Statistical Procedures
Statistical analysis of the data was carried out with SPSS version 22.0 using One Way Analysis of Variance (ANOVA). The statistically analyzed data were reported as Mean ± SD. Significant difference was accepted at 95% confidence level of probability i.e. if P < 0.05.

Effects of Consumption on Haematological Parameters
The result of the effect of the pesticide on haematological indices is presented in Table 1 below. The WBC result showed non-significant increase for groups 1 and group 3 i.e. groups with high concentration of DDVP and a non-significant decrease for groups 2 and group 4 i.e. groups with low concentration of DDVP.

Effects of Consumption on Antioxidant Balance
The glutathione (GSH) significantly decreased in the un-parboiled groups (i.e. group 3 and group 4) but non-significantly decreased in the parboiled groups (group 1 and group 2) when compared to the control as shown in Figure 1, catalase (CAT) activity decreased significantly in the un-parboiled groups (i.e. group 3 and group 4) but non-significantly decreased in the parboiled groups (group 1 and group 2) when compared to the control (Figure 2). The glutathione peroxidase (GPx) significantly decreased in the un-parboiled groups (i.e. group 3 and group 4) but non-significantly decreased in the parboiled groups (group 1 and group 2) when compared to the control as seen in Figure 3. The superoxide dismutase (SOD) showed a significant decrease in group 1, group 3 and group 4 but a non-significant decrease in group 2 when compared the positive control, group 6 as seen in Figure 4. The malondealdehyde (MDA) levels increased non-significantly in group 1 and group 2 i.e. the parboiled groups but significantly increased for group 3 and group 4 when compared to the positive control as shown in Figure 5. Figure 1. Serum glutathione activity in Wistar rats of different groups. From the chart above, there is significant (P < 0.05) increase when group 6 (9.87 ± 0.46) is compared to group 3 (5.97 ± 0.49) and group 4 (6.3 ± 0.36) but a non-significant (P < 0.05) increase is seen when group 6 is compared to group 1 (8.28 ± 0.38), group 2 (7.21 ± 0.28) and group 5 (8.52 ± 0.62).   Figure, the comparisons of group 6 (4.30 ± 0.12) to group 5 (3.80 ± 0.17), group 2 (3.15 ± 0.25) and group 1 (3.34 ± 0.14) show a non-significant (P < 0.05) decrease but when group 6 is compared to group 3 (1.52 ± 0.12) and group 4 (2.20 ± 0.16), it shows a significant (P < 0.05) decrease. Figure 4. Effect of dichlorvos on serum superoxidedismutase activity in Wistar rats. From the chart above, the comparison of group 6 (11.02 ± 0.04) to group 1 (10.69 ± 0.04), group 3 (10.43 ± 0.05) and group 4 (10.59 ± 0.05) shows a significant (P < 0.05) increase but shows a non-significant (P < 0.05) increase when compared to group 2 (10.79 ± 0.05) and group 5 (10.99 ± 0.03). Figure 5. Effect of dichlorvos on serum totalmalondialdehyde concentrationin Wistar rats. From the chart above, there is non-significant (P < 0.05) decrease in concentration of malondialdehyde when group 6 (0.62 ± 0.07) is compared to group 1 (0.85 ± 0.05), group 2 (0.87 ± 0.06) and group 5 (0.80 ± 0.05). But a significant decrease (P < 0.05) is seen when group 6 is compared to group 3 (1.49 ± 0.03) and group 4 (1.08 ± 0.04).

Discussion
Research on exposure [23], [24] and administration [25] of dichlorvos has shown that reactive oxygen species (ROS) are generated by dichlorvos thereby inducing oxidative stress. Prakasam [26] and Hsu [27] reported that excessive free radical production resulting in oxidative stress could be an important mechanism of organophosphate toxicity. From this study, glutathione which is usually the first line of defense against oxidative stress is significantly decreased when the treatment groups are compared to the control which is an indication that there is an increase in the levels of ROS generated in the cell [28] thereby leading to increased oxidative stress. It has been shown that certain environmental toxicants can elicit oxidative stress by converting the parent compound to its reactive metabolites [29]. Reduction in the GSH level may be due to indirect conjugation with excess electrophiles produced due to dichlorvos [13].
There is a significant decrease in the level of catalase (CAT) and glutathione peroxidase (GPx). The present result contradicts the reports of Kanu [24], Dwivedi [30] and [31] were the antioxidant enzymes were reported to have increased.
There was a slight increase in the Malondealdehyde (MDA) levels which is a biomarker for lipid peroxidation (LPO) although the increase was not significant in all the groups except group 3 which showed a significant increase. Pesticides are known to move from treated agricultural areas into the broader environment, and then they can reach to the non-target organisms [33] implying that its usage may lead to undesirable residues as trace contaminants of food, the environment and living tissues hence, non-significant decrease (P < 0.05) in group 4 suggests that the toxicity of dichlorvos may be dose-dependent and the parboiling of the beans may further reduce the concentration of pesticide residues deposited on the beans since the uncontaminated beans i.e. group 5 showed a significant increase when compared to the control.
The hematological parameters showed a dose dependent result which could have resulted from the levels of reactive oxygen species generated in the blood.
In the report of the research carried out by Akomas [34], it was observed that there was the development of hypochronicanaemia due to a fall in the iron content of the body resulting from oxidative stress. Exposure to dichlorvos induces oxidative stress by generation of reactive oxygen species [23]. Oxidative stress occurs when the production of harmful molecules called free radicals is beyond the protective capability of the antioxidant defenses [35]. Disruption of the activities of antioxidants suggests alteration of the oxidative state of blood cells by the pesticide [24]. Therefore, this present study suggests that the consumption of dichlorvos can disrupt the antioxidant and pro-oxidant balance.

Conclusions
In summary, owing to this research work, dichlorvos has been seen to possess some level of toxicity when the dosage is not considered since it can induce oxi-N. Chinedu, N. Darlington DOI: 10.4236/jbm.2019.711006 Journal of Biosciences and Medicines dative stress by generating reactive oxygen species. Despite its relevance in agriculture (Pre-harvest and pro-harvest) in preserving stored products, when applied, a fraction of dichlorvos reaches to target pest while the rest enters into the food chain and leads to toxicity of different organs of the body and can be lethal overtime.
Unlike the parboiled groups, un-parboiled groups showed a significant decrease in the levels of the antioxidant enzymes which suggests the need of thorough washing and parboiling of beans during its preparation before consumption. Also, there is need to sparingly air-dry our beans in order to allow some of the chemicals used in its preservation to diffuse out completely since the comparison of group 5 (normal beans), negative control showed no significant difference in the antioxidant levels when compared to group 6.
The haematological parameters showed dose-dependent differences where groups with higher concentrations of dichlorvos varied from the control. Hence, there should be appropriate restriction and monitoring of the usage of dichlorvos based pesticides to ensure the right dosage meted out since dichlorvos is electrophilic in nature. Also, there should be an appropriate consideration before the application of dichlorvos preservative since some people parboil their beans while others do not parboil theirs although this research elucidates the need to parboil beans during its preparation.