Study of Cofactors Associated with Precancerous High-Grade Cervical Lesions at the Teaching Hospital Gabriel Touré, 2010 to 2015

Introduction: High grade dysplasia of the cervix has a high incidence and can progress to cervical cancer. The aim was to study cofactors associated with high-grade cervical dysplasia. Methodology: This was a retrospective case-control study without matching. Women with high grade dysplasia were the cases while those with a normal screening test represented the controls. The study took place at the Gabriel Touré University Hospital Center in Bamako. We included 351 cases and 420 controls. The capture and analysis were performed using the SPSS 20 software. A univariate and multivariate logistic regression analysis was performed for the analysis of risk cofactors. The statistical tests used were the odds ratio and its confidence interval and the statistical significance threshold was set at p < 0.05. Results: In univariate analysis, the co-factors statistically significantly associated with the occurrence of high-grade dysplasia were parity 0.6 (0.5 - 0.9), gestational 0.7 (0.5 - 0.9), smoking of the spouse 3.4 (1.1 - 11.3), the non-schooling 1.4 (1.2 - 2.1). In multivariate analysis after adjusting for confounding factors, two co-factors have significantly increased the risk of high-grade dysplasia: lack of schooling 1.4 (1.2 - 2.0) and polygamy 1.5 (1.4 - 2.5). Conclusion: At the end of this study, polygamy and lack of schooling were the main risk factors. The prevention of cervical cancer will go through the education of girls and women as well as communication for behavioral change and social change.


Introduction
Precancerous high-grade lesions or high-grade dysplasia of the cervix constitute the disorganization of the architecture of the squamous epithelium with loss of normal maturation and stratification, cytonuclear atypia and mitosis reaching the middle third and superior to the epithelium and respect the basement membrane [1] [2]. High-grade dysplasia consists of intraepithelial neoplasia of the second-degree cervix (CIN2) and intraepithelial neoplasia of the third-degree cervix. (CIN3). Low grade dysplasia is composed of first-degree cervical intraepithelial neoplasia (CIN1). High grade dysplasia and cervical cancer are non-transmissible pathology of infectious origin [3] [4]. Persistent infection of high-risk human papillomavirus (HPV) oncogene is considered the main but not sufficient cause alone for causing high-grade dysplasia and cervical cancer [3] [5]. These diseases have been recognized by the World Health Organization (WHO) as being 100% attributable to HPV infection [6]. The natural history of cervical cancer occurs along the continuum of precancerous histological lesions or cervical intraepithelial neoplasia (CIN). These follow the persistence of infection with high-risk oncogenic HPV for each grade of the precancerous cervical lesion (CIN1, CIN2, CIN3), there is a probability of regression that varies by 57%; 43%; 32%. In addition, the probability of persistence of CIN1, CIN2, CIN3 is 32%; 35%; 56%. In fact, the probability of progression of CIN1, CIN2, CIN3 towards invasive cancer is also 1%; 5%; and greater than 12% [3] [7] [8]. High grade dysplasia and cervical cancer pose a major public health problem because of their high incidence and high mortality from cervical cancer. The specific role of cofactors in the development of high-grade dysplasia and cervical cancer is not fully understood [3] [5]. This scientific deficit in the carcinogenesis of cervical cancer motivated us to undertake the present study in Mali where resources are limited and the burden of cervical cancer is enormous [6]. Previous studies in Mali on cervical cancer are mainly descriptive because of the scarce resources allocated to research and the poor quality of existing data in our structure's health. It is in this context that we initiated this work to study cofactors associated with precancerous high-grade cervical lesions.
The specific objectives of this study were: • describe the general characteristics of cases and controls, • compare the characteristics of participants with high grade dysplasia to those with no dysplasia or cervical cancer. To clarify the influence of different cofactors we asked the following research question: Are women with high grade dysplasia more risk cofactors than those free of dysplasia?
The objectives of this study will be achieved by verifying the following research hypothesis: Cofactors increase the risk of high-grade cervical dysplasia.

Study Framework
This study was conducted at Gabriel Touré Hospital Center in Bamako in the

Type and Period of Study
We performed an unpaired case-control study. Data collected included women who visited between January 1, 2010 and October 31, 2015.

Study Population
It consisted of all sexually active women who received cervical cancer screening during the study period. These were referred or evacuated women from the dis-

Sampling
Were included in this study all women who met the following criteria: • Inclusion Criteria: Included in this study were all women who met the following criteria: -sexually active woman who has been screened for cancer of the cervix; -free from dysplasia or cervical cancer confirmed by histology; -woman with high grade dysplasia confirmed histology; -woman having a record exploitable. • Exclusion criteria -Woman having a total hysterectomy; -Virgin woman; -Woman with cervical cancer confirmed by histology; -Woman whose histology was not performed; -Woman with an unexplainable record.
• Sampling technique: Cases and controls were defined as follows: Definition of cases: it included any sexually active woman whose biopsy has shown a high-grade cervical lesion was a case.
Definition of controls: it included any sexually active woman whose histology has not confirmed any dysplasia or cervical cancer.
• Minimal height of sampling A full sampling was conducted from 2010 to 2015. We have defined certain values to make the calculation of the minimal height of the sample. The number of controls was given in a study conducted by Bayo et al. [9] in 2002 in Bamako which found 14% of large multiparty with witnesses (one case for one control). Analysis and data processing strategy: The data analysis and processing was made by SPSS version 20 software. The cases and the witnesses were separated during this process. The data were analyzed to make sure of the speed of distribution. We conducted a univariate and multivariate study.

Descriptive Epidemiology
We collected 351 women with dysplasia of high and 420 subjects free from dysplasia or cancer. Women with high grade dysplasia had a middle and median age of 37 with a minimum age of 18 and a maximum age of 73 years. Women with dysplasia or cancer had an average age of 39 years and a median age of 40 with extremes ranging from 18 to 75 years. The difference between the mean age of cases and the average age of control was significant (t = 2.034, p = 0.042).
We also specified the distribution of the bacteria found in PV in both groups (Table 2).
We screened 12,623 women among whom were collected 351 cases and 420 controls for a period of 6 years as shown in the flowchart (Figure 1).
The distribution of precancerous lesions of the cervix depending on age is shown in Figure 2.
The 30 -39 age group was more concerned with precancerous high-grade and low-grade cervical lesions. However, adolescents (age ≤ 19 years) were poorly affected by these precancerous lesions ( Figure 2). The extreme ages of precancerous

Analytical Epidemiology
Average age of women with high grade dysplasia We compared the average age of women with high grade dysplasia to those free from dysplasia or cancer. In fact, the average age of women with high grade dysplasia was 37 ± 0.5 years, while that of patients without dysplasia was 39 ± 0.5 years. The difference between these two average ages was significant (t = 2.034, p = 0.042). The analysis of the variance confirmed the results of the test. It showed that the average age of patients with high grade dysplasia was statistically different from that of free from dysplasia or cancer (Anova F = 3.330, p = 0.036).

Risk factors
The risk factors have been studied in Table 3 below.

Discussion and Comments
In the light of the work of the literature, the main results of this work have been commented upon and discussed.

Limitations of the Study
This study presented some methodological limitations. As the data collection was retrospective, not all independent variables could be fully informed. Indeed, all women with IVA and or IVL negative were excluded because it is not ethical to practice biopsy on a normal cervix. However, these women with IVA negative and or IVL negative could be real witnesses. Pathological examination was selected as a reference test for the diagnosis of high-grade dysplasia. This was an  analytical study to identify associated factors contributing to precancerous lesions. Understanding the role of co-factors would enable field health workers to better develop key messages for the population. This work attempts to explain the recent epidemiology of high-grade dysplasia and cervical cancer which is the rejuvenation of patients with these conditions. This study will prevent cancer of the cervix.

Prevalence of High-Grade Precancerous Lesions
We collected 351 cases of high-grade dysplasia on 12,623 women who had cervical cancer screening, a prevalence of high-grade dysplasia of 2.8% ( Figure 1).

Average Age of Women with High Grade Dysplasia
The average age of women with high grade dysplasia was 37 ± 0.5 years compared to 39 ± 0.5 years for free from dysplasia or cancer. These age averages are lower than those of Bayo et al. [9] who found 46 years for cases and controls. Khaoula, B. et al. [11] found an average of 46 ± 8.7 years for high grade dysplasia in a descriptive study in Morocco. The epidemiology of precancerous and cancerous lesions is being modified. Indeed, the average age of CIN was 30 years

Marital Status
In our study, polygamous women with spouses with more than two wives were  [12]. A study by Tegueté et al. [12] confirmed that the risk
The risk of developing high-grade cervical lesion by these out-of-school women is related to under-reporting and lack of cervical cancer screening. These reasons make oncogenic HPV infection become common and persistent. Low levels of knowledge and perception in the general population have been reported up to 60% of women had never heard from about cervical cancer and only 7.8% had heard about it.

Conclusion
Effective prevention of cervical cancer requires active research, screening, and, where appropriate, management of the co-wives of women with high-grade cer-