Functional and Structural Arterial Vessel Features of Female Patients with Stable Dermatomyositis and Antisynthetase Syndrome

Introduction: Dermatomyositis (DM) and antisynthetase syndrome (ASS) show a high frequency of metabolic syndrome, which can be preceded by endothelial dysfunction and arterial stiffness. To date, only one study has evaluated these vessel parameters in DM, and no study of ASS exists. Therefore, the aim of the study was to assess the structural and functional arterial of arterial vessels in DM and ASS. Methods: This cross-sectional study enrolled 21 adult female patients (14 DM and 7 ASS) who were age-, gender- and eth-nicity-matched to 12 healthy individuals. Patients using lipid lowering agents or prednisone at doses ≥ 0.25 mg/kg/day, and patients with uncontrolled systemic arterial hypertension, diabetes mellitus, cardiac insufficiency, and disease activity were excluded. Arterial stiffness was evaluated using caro-tid-femoral pulse wave velocity (PWV), and endothelial function was evaluated using dependent flow-mediated dilatation (FMD) of the brachial ble DM and ASS had FMD and PWV values comparable to those of the control group.

Physiopathologically, atherosclerosis is characterized by early endothelial dysfunction and increased arterial stiffness [4] [5]. In this context, flow-mediated vasodilatation (FMD) and pulse wave velocity (PWV) assessments have been used extensively in clinical practice as non-invasive, validated and reproducible techniques for subclinical atherosclerosis assessment [4] [5].
In the only available study in the literature, Vincze et al. [7] demonstrated the impairment of dependent FMD and increased arterial stiffness in patients with DM. However, the authors did not provide details about the patients' age, gender, clinical and laboratory features or treatment. Because these parameters can interfere with FMD and arterial stiffness values, this study's results should be analyzed with caution. Finally, to the best our knowledge, no study has assessed FMD and PWV in patients with ASS.
Therefore, the goal of the present study was to assess the structural and functional behavior of arterial vessels in patients with stable forms of DM or ASS, as compared with age-, gender-and ethnicity-matched to healthy individuals. College of Rheumatology (EULAR/ACR 2017) classification criteria for SAMs [11]. Definition of ASS was based on the study of Connors et al. [3].

Exclusion Criteria
Patients with clinical and laboratory disease activity, overlapped syndromes, diabetes mellitus, uncontrolled systemic arterial hypertension, or cardiac insufficiency were excluded, as were patients using lipid-lowering agent or prednisone at doses of ≥0.25 mg/kg/day in the last 3 consecutive months.

Patients' Data
The following data were obtained from the patients: 1) Demographic data: current age, gender and body mass index (BMI) (kg/m 2 ).
3) Disease parameters: disease duration and current disease status assessed using the International Myositis Assessment & Clinical Studies Group (IMACS) set scores: Manual Muscle Testing (MMT)-8, Myositis Disease Activity Assessment Visual Analogue Scales (MYOACT), global assessment of disease by physician and patient through the Visual Analogue Scales (VAS), Health Assessment Questionnaire (HAQ), and serum levels of the muscle enzymes [12]- [17].
4) Treatment: current treatments, including glucocorticoid and immunosuppressive drugs. 5) Comorbidities: systemic arterial hypertension and dyslipidemia: total cholesterol > 200 mg/dL, high density lipoprotein (HDL)-cholesterol < 40 mg/dL and/or low density lipoprotein (LDL)-cholesterol > 130 mg/dL [18]. 6) Endothelial function assessment: The test was performed in the morning, after the patients had fasted for 8 hours. They were tested and on the right arm. The patients were instructed to remain fasting and without vasoactive medications for 12 h before evaluation. The environment of the examination was silent, with low luminosity and maintained temperature between 20˚C and 25˚C. Images of the right brachial artery located 7 cm above the medial humeral epicondyle were recorded for measurements of blood flow velocity and arterial resting diameter. For FMD (endothelium-dependent) register, a sphygmomanometer was placed on the forearm and inflated to a pressure of at least 50 mmHg above systemic pressure for 5 min, inducing reactive hyperemia maneuver. Images were captured during 3 min after cuff release. The diameter of the artery was through ultrasound equipment (Sequoia Echocardiography System ® , version 6.0, Siemens, CA, USA). The different phase of the examination was recorded on computer for later analysis. FMD% (endothelium-dependent) was calculated according to the formula below: FMD% = Diameter after hyperemia − Resting Open Journal of Rheumatology and Autoimmune Diseases diameter × 100/Resting diameter. Moreover, four healthy subjects were recruited to measure inter-observer reproducibility, which were evaluated at different times of the day by the same evaluator. 7) Aortic stiffness assessment: Before the evaluation, the patients performed 5 minutes of rest in dorsal decubitus in silence. Later, their systolic blood pressure (SBP) and the diastolic blood pressure (DBP) values were assessed using automatic non-invasive PWV (m/s) measurements by the Complior ® device (Colson, Gargesles Gonesses, France). Two typing scanners (TY 306, Tokyo, Japan) were positioned simultaneously in the carotid and femoral arteries, located at a previously measured distance. These sensors contain membranes which are successively deformed by the shock of the pulse wave, and this deformation is initially transformed into an electrical signal and transmitted to a computerized calculation program.
PWV was analyzed by means of the carotid-femoral trajectory. Each pulsating wave appears real time on the computer screen, and the apparatus determines through the tangent to the initial ascending phase of the pulse wave, the beginning of the wave at the two locations and deduces, as a function of the distance between the two points, measured with tape measure, the pulse wave velocity, i.e., PWV = L/dL to the time delay between the pulse waves. To obtain the PWV value, 10 curves were considered and the mean was calculated. The curves were acquired with the patients in horizontal dorsal decubitus and analyzed by an experienced and independent evaluator.
The control (CTR) group was composed of employees from our tertiary center, who were age-, gender-and ethnicity-matched to patients with DM and patients with ASS.
Statistical Analysis The normality of the data was verified by Shapiro Wilk adherence test with described level of α = 0.5. ANOVA or Kruskal Wallis test was utilized for comparison between DM, ASS and CTR group. Whereas, Student's t-test or Mann Whitney test were used for comparison between independent means between the DM and ASS groups. To verify the reproducibility of FMD measurement the intra-class correlation coefficient was used. Fisher's exact test and chi-square test were used for the categorical variables of two independent groups. P value ≤ 0.05 was considered statistically significant. The software used was the SPSS 22 (Chicago, IL, USA).

Results
Forty-four out of 91 patients fulfilled the present study's inclusion criteria. However, 23 patients refused to participate, due to personal motivations, work schedules, resulting in 21 female participants: 14 DM patients and 7 ASS patients. These patients were age-, gender-and ethnicity-matched to 12 individuals from the CTR group. The patients and CTR group had a comparable mean age: DM (45.4 ± 8.6 years), ASS (44.0 ± 6.1 years) and CTR (43.9 ± 7.2 years), and were predomi-Open Journal of Rheumatology and Autoimmune Diseases nantly of white ethnicity. The mean disease duration was 6.0 years (DM: 5.3 ± 3.5 years and ASS: 6.1 ± 2.8 years), as shown in Table 1. BMI varied among patients    with ASS and patients with DM, as well as in the CTR group (P = 0.039). However, patients with ASS and patients with DM had similar BMIs (P = 0.617). Patients with ASS and patients with DM had stable diseases (Table 2) according to the IMACS set scores. Moreover, only 4 patients with DM used prednisone, and the median dose was 2.5 mg/day. No ASS patients used glucocorticoids. In general, 85.5% of patients with DM and 71.4% of patients with ASS used at least one immunosuppressive, immunomodulatory or immnobiological drug (azathioprine, methotrexate, mycophenolate mofetil, leflunomide and/or rituximab). Regarding other drugs, 42.8% of patients with DM and 42.8% of patients with ASS used an anti-hypertensive drug (beta blocker or calcium channel blocker) ( Table 2).
Concerning comorbidities, 6 out of 14 DM patients, and 3 out of 7 ASS patients had systemic arterial hypertension. Dyslipidemia was identified in 9 out of 14 patients with DM, and 6 out of 7 patients with ASS.
Endothelial baseline diameter (P = 0.130), hyperemia diameter (P = 0.282), and FMD values (P = 0.451) were similar among the ASS, DM and CTR groups. Moreover, FMD values were also similar in patients with DM and patients ASS (P = 0.585) (Figure 1).
PWV values were also similar among patients in the DM, ASS and CTR groups (P = 0.253). In addition, there was no difference between patients with DM and patients with ASS (P ≥ 0.999) (Figure 2).

Discussion
In the present study, we found no differences in the structural and functional characteristics of large arterial vessels in adult females with stable forms of DM and ASS and healthy individuals.   Our patients were submitted to strict exclusion criteria to avoid any confounding factors that could interfere with the interpretation of our results. For instance, we included only patients with stable diseases, described pharmacological treatment details, and excluded patients with diabetes mellitus and uncontrolled systemic arterial hypertension. Furthermore, the patients were age-, gender-and ethnicity-matched to members of the CTR group.
In this context, there were no differences between patients with DM and patients with ASS in relation to the CTR group. Likewise, in a sub-analysis the vascular parameters were comparable between patients with DM and patients with ASS.
Our results corroborate the only one available study in the literature [19] which showed no differences in the PWV and FMD of DM patient's parameters compared to a healthy control group. However, we specify patients' clinical, laboratory, pharmacological treatment, and disease status, and the patients matched by age, gender and ethnicity variables.
In this context, several studies have identified associations between disease status and the worsening of vascular structure and function [20] [21]. However, the chronic use of glucocorticoids results in increased risk factors for cardiovascular diseases, particularly obesity, diabetes and dyslipidemia [22] [23].
In an additional analysis, that considering only patients with DM, Vincze et al. [7] observed a reduction in the patients' FMD values when compared to those of healthy individuals. However, in this additional analysis, characteristics related to the disease status and the patients' general characteristics were not described, and the study did not mention whether the patients' general characteristics were matched to the CTR group [19]. In our study, we observed in a sub-analysis that the values of DM and ASS patients' FMD and PWV values were comparable to those of the CTR group, which was analyzed separately and matched by age, gender and ethnicity. This provided reliable results.
Our study's results suggest that stable SAMs with dyslipidemia or systemic arterial hypertension do not promote eventual endothelial dysfunction or in-Open Journal of Rheumatology and Autoimmune Diseases creased arterial stiffness per se as previously reported in some studies [21].
Regarding our study's limitations, the number of patients in the present study precludes any population stratification. In addition, we did not analyze other variables related to endothelial function (i.e., menstrual cycle, retrograde and anterograde flow), so we cannot propose an overall understanding of the functional arterial parameters. Given these limitations, it is eminently necessary to carry out studies that examine such variables.
In conclusion, there are no differences in the structural and functional parameters of large arterial vessels in adult females with stable forms of DM and ASS, when compared to healthy individuals.