Impact of Therapeutic Education on the Viral Load of HIV Infected Children and Adolescents on Antiretroviral Therapy at the Douala Laquintinie Hospital , Cameroon

Introduction: One of the biggest challenges for HIV-infected adolescents on antiretroviral therapy (ART) is the long-term maintenance of viral suppression, which is the third 90% goal of UNAIDS. Therapeutic Education (TE), process of acquiring abilities and skills that help the patient to live optimally with his illness is one of the strategies that contribute to the achievement of viral suppression through the therapeutic adhesion contract and the followup of the patient. The aim of this study was to evaluate the impact of TE on the virologic response of children and adolescents aged 8 19 under ART and followed up at the Laquintinie Hospital of Douala (LHD). Method: A crosssectional study was conducted at the Pediatric Unit of the HIV/AIDS Accredited Treatment Center (ATC) at LHD from February to May 2016. Children and adolescents aged 8 to 19 years on ART, followed in ATC/LHD whose parents had agreed to participate in the study, and who had achieved at least one viral load before and after initiation of TE, were recruited consecutively during routine medical follow-up. Data were collected from patients’ medical How to cite this paper: Penda, C.I., Bissek, A.-C.Z.-K., Bilong, S.C., Boupda, L.-A., Okala, C., Ndongo, F.A., Ngondi, G.D., Eboumbou, E.C.M., Njock, L.R. and Ndombo, O.K. (2019) Impact of Therapeutic Education on the Viral Load of HIV Infected Children and Adolescents on Antiretroviral Therapy at the Douala Laquintinie Hospital, Cameroon. International Journal of Clinical Medicine, 10, 109-121. https://doi.org/10.4236/ijcm.2019.103011 Received: January 31, 2019 Accepted: March 4, 2019 Published: March 7, 2019


Introduction
Infection with pediatric Human Immunodeficiency Virus (HIV) remains a public health priority.Of 1.8 million children under the age of 15 living with HIV worldwide in 2016, almost 90% lived in sub-Saharan Africa [1].With antiretroviral therapy, an increasing number of children infected with HIV through vertical transmission in Africa are living longer and reaching adolescence [2] [3] [4] [5].One of the biggest challenges in this adolescent population is the long-term maintenance of therapeutic compliance to achieve viral suppression.
Cameroon has adhered to the strategic Objectives 90-90-90 of United Nations Program on HIV/AIDS (UNAIDS) [6], which aims to increase HIV testing to 90%, achieve ART coverage of 90% and 90% of people on antiretroviral therapy (ART) achieve viral suppression by 2020.Therapeutic Education (TE), process of acquiring abilities and skills that help the patient to live optimally with his illness, is one of the strategies proposed by achieve viral suppression [7].For HIV infected children and adolescents, it consists in transmitting to the patient on ART the skills that help him to live optimally with his illness and to improve the therapeutic observance.It integrates the process of announcing HIV status in children and adolescents [7].In children, the disclosure of HIV status is a progressive and delicate process considering their cognitive level and their emotional state [8].The beneficial effects of TE on mental health and adherence [9] [10] may help to mitigate the low retention of care seen in children and adolescents on ART [11] [12].However, few studies have been conducted to evaluate the efficacy of TE on the therapeutic response of children and adolescents on ART in Since 2011, "the therapeutic school" has been set up at the Accredited HIV/ AIDS Treatment Center (ATC) of LHD to transmit therapeutic self-management skills to children and adolescents on ART.The aim of this study was to evaluate the impact of TE on the virologic response of children and adolescents aged 8 -19 under ART and monitored at the ATC/LHD.

Study Design
A cross-sectional study was conducted at the pediatric unit of the ATC/LHD during the period from February to May 2016 (4 months).It is a center of excellence for pediatric HIV care covering an active line of 452 children and adolescents at the time of the study.

Study Population
Children and adolescents aged 8 to 19 on ART, followed at ATC/LHD whose parents accepted the study and had at least one viral load before and after the start of participation in the study.Therapeutic School were recruited consecutively during routine medical consultations.

Establishment of the Therapeutic School at Laquintinie
Hospital in Douala

Definition of Operational Terms
The assessment of the scale of knowledge for the acquisition of the notions transmitted to the therapeutic education was defined according to the age based on a score graduated from 0 to 10.
Was considered as patient with a good knowledge, patients who obtained: • a score of 8 to 10 for children aged 8 to 10; • a score of 11 to 15 for children aged 11 to 14; • a score of 16 and 20 for children aged 15 to 19.

For average knowledge:
• a score of 5 to 7 for children aged 8 to 10; • a score of 7 to 10 for children aged 11 to 14; • a score of 11 and 15 for children aged 15 to 19.

For bad knowledge:
• a score of 0 to 4 for children aged 8 to 10; • a score of 0 to 6 for children aged 11 to 14; • a score of 0 and 10 for children aged 15 to 19.

Data Collection Procedure
An informed consent form was provided to the parent or legal guardian of eligible children and teens for the study, as well as a consent form for adolescents over the age of 14 in the waiting room for consultations, medical or pharmaceutical, during the dispensing of drugs.For unaccompanied children and adolescents, an informed consent form was given to them to give to their parent or legal guardian.In addition, a telephone conversation was sometimes necessary to provide them with additional information for a better understanding of the study.After obtaining the parent's consent and, if applicable, the adolescent's consent, an age-appropriate assessment skills questionnaire was provided to the participant at a time.Monthly session of the therapeutic school or in the waiting room for medical consultations or during the dispensing of medicines from the pharmacy.The participant completed the questionnaire alone for a maximum of 15 minutes.At the same time, the other study data were collected in the participant's medical record.

Variables
The main variable of interest was the change in viral load (VL), measured before and after the initiation of TE sessions, defined as a binary qualitative variable of modalities.VL detectable after initiation of the TE or VL undetectable after init- The main explanatory variables included the type of TE (individual-only, or individual and collective) and the number of TE sessions administered (≤10 sessions or >10 sessions).
Secondary explanatory variables related to characteristics of the child or adolescent (age (≤14 years or >14 years), degree of HIV status announcement (partial or complete), level of education (illiterate/primary or secondary/superior)) and the caregiver of the child or adolescent (type of relationship with the child or adolescent (father/mother or guardian), HIV status (negative or positive)).

Statistical Analysis
The descriptive analysis consisted in estimating the size of the categories of the qualitative variables and the average of the quantitative variables associated with the standard deviation.
The association between the explanatory variables and the evolution of the viral load in children or adolescents who initiated TE was studied by univariate and multivariate analyzes using logistic regression models.These analyses were performed separately in groups 1 (undetectable viral load before initiation of TE) and 2 (detectable viral load after initiation of TE).Odds ratios between the categories of exposure variables were compared using the Score test.The level of education, the degree of announcement of HIV status, the type and number of therapeutic education sessions administered were included in all multivariate models (a priori exposure factors

Skills Acquired during TE Sessions
At the time of this study, the knowledge of the importance of taking medicines was acquired by 86.1% of children 8 -10 years and the effect of drowsiness of the virus by taking the drug by 75.0%(Table 2).Respectively 95.4% and 97.3% of adolescents aged 11 -14 years and 15 -19 respectively knew the importance of taking medication schedules.About three-quarters (74.7%) of adolescents aged 15 -19 were able to interpret the result of their viral load while a smaller proportion of them (58.7%)knew how to interpret the result of the CD4 count (Table 2).

Significant Association between the Evolution of Viral Load and the Lower Number of FTE Learning Sessions
In group 1 (undetectable viral load prior to TE initiation), the only exposure factor significantly associated with maintaining undetectable viral load after initiation of TE in the univariate analysis was receiving less than 10 TE sessions (OR = 2.6 (1.1-5.9),p = 0.02) (Table 3).In multivariate analysis, having less than International Journal of Clinical Medicine    ), p = 0.03) while a non-significant association was noted in children and adolescents living with their father or mother (OR = 2.2 (0.9 -5.2), p = 0.07).There was no association between the maintenance of an undetectable viral load after initiation of TE and the HIV status of the person in charge of child or adolescent care, the type of TE administered, the level of education, the degree of announcement of HIV status and the age of the child or adolescent.
In group 2 (detectable load prior to TE initiation), no exposure factor was associated with changes in viral load after initiation of TE (Table 4).

Discussion
This study evaluated the impact of therapeutic education on the virologic response in 198 children and adolescents aged 8 to 19 years on ART and monitored at LHD.In this study population, 86.1% of children aged 8 -10 years had acquired knowledge of the importance of taking medications, 95.4% and 97.3% of adolescents in the 11 -14-year-old and 15 -19-year-old age groups respectively of medication schedules.These results are comparable to those of another study conducted among Cameroonian children [13].Among children and adolescents with undetectable viral load prior to initiation of TE, 76.5% maintained an undetectable viral load after initiation of TE.In addition, 72.3% of those whose viral load was detectable before initiation of TE had acquired an undetectable or decreasing viral load after initiation of TE.These results show the beneficial effect of TE on achieving or maintaining an undetectable viral load.Indeed, therapeutic education had positively influenced therapeutic compliance in a cohort of Romanian children and adolescents [10].
The only exposure factor significantly associated with maintaining undetectable viral load after initiation of TE was having less than 10 TE sessions.This result seems to reflect a better virologic response in subjects who acquire skills faster through TE and therefore require fewer learning sessions to adapt.In addition, the nonsignificant association between maintaining undetectable viral load and living with his father or mother may be due to a lack of power in our study.A larger population of children with undetectable viral loads prior to TE initiation would result in a statistically significant association.In a Ugandan study [14], motherless children did not have adequate physical, mental, and social support at home.This situation may hinder the effectiveness of TE in children living with a guardian.In addition, the death of the parents during the TE process may promote an alteration of therapeutic compliance and partially justify the proportion of subjects who were unable to maintain their viral load at an undetectable level after initiation of the treatment.In this study, parental mortality was not described throughout the TE process.
In our study, the degree of disclosure was not associated with changes in viral load, contrasting with a Romanian study [10] that showed a significant associa- HIV infected children on ART.The difference in these results could be explained by the fact that in our study population, all subjects had already received a partial or total announcement of their status according to their level of cognitive maturity and their emotional state.
The lack of association between undetectable viral load after initiation of TE and different risk factors in the group of children and adolescents with detectable viral load prior to initiation of TE could be explained by a lack of statistical power.Indeed, the size of this group (N = 65) was relatively small.

Conclusion
The level of acquisition of skills and knowledge through TE was satisfactory among children and adolescents on antiretroviral therapy in this study.In addition, the TE had a favorable influence on maintaining or achieving undetectable viral load.As a result, TE effectively contributes to achieving the third 90% goal of UNAIDS and to improving pediatric HIV care in Cameroon.

Contributions of Authors
• CIP, ACZKB, SCB, OKN and LRN concepted and designed the study.
• All authors approved of the final version.
This study was conducted in accordance with ethics regulations for research on humans in Cameroon.An ethical clearance was obtained from the Institutional review Board of the University of Douala prior to the start of the study (N˚/CEI-UDo/740/01/2017/T) and administrative authorization (N˚0010/AR/ MINSANTE/DHL/CM) was obtained from the LHD.Before enrolment and the administration of questionnaire, parents or legal guardians were informed on the purpose and process of the investigation (background, goals, methodology, International Journal of Clinical Medicine study constraints, respect of privacy and data confidentiality, and rights to opt out from the study), and a signed informed assent was obtained from all parents or legal guardians for inclusion and use of anonymous data of their children in publication and conference presentations.Participation was voluntary, anonymous and without compensation.

C
. I. Penda et al.DOI: 10.4236/ijcm.2019.103011119 International Journal of Clinical Medicine tion between the lack of disclosure of HIV status and the increase in viral load in A total of 198 children and adolescents were included in this study at an average age of 14 years (±3).The 8 -10, 11 -14 and 15 -19 age groups accounted for 17.2%, 44.9% and 37.9% of the study population respectively (Table1).Of these children and adolescents, just under half (48.0%) were male, the vast majority (91.9%) resided in the city of Douala, almost two-thirds (69.7%) were at a level of secondary education.More than half (56.1%) had already received the full announcement of their HIV status.Children with both parents living accounted for 37.9% and 40.4% were fatherless or motherless.The person in charge of child or adolescent care was the mother in 48.5% of the cases and her HIV status was positive in (54.5%) of the cases.

Table 1 .
Sociodemographic characteristics of children and adolescents before therapeutic education (LHD, Cameroon, February-May 2016).
LHD: Laquintinie Hospital of Douala; n: Number of children or adolescents in the approved category of the variable; %: percentage; N: Total number of children/adolescents. International Journal of Clinical Medicine

Table 2 .
Knowledge and skills acquired by children and adolescents during TE sessions (LHD, Cameroon, February-May 2016).
TE: Therapeutic Education; LHD: Laquintinie Hospital of Douala; n: Number of children/adolescents in the approved category of variable; %: percentage; N: Total number of children/adolescents.

Table 3 .
Evolution of viral load among children and adolescents with undetectable viral load before initiation of therapeutic education (LHD, Cameroon, February-May 2016), N = 133.: Therapeutic Education, LHD: Laquintinie Hospital of Douala; Group 1: Children and adolescents with undetectable viral load before TE; Group 2: Children and adolescents with detectable viral load before TE; VL: Viral load; n: Number of children or adolescents belong to the category considered of the variable; %: percentage; N: Total number of children or adolescents; OR: Odds Ratio; 95% CI: 95% Confidence Interval; p: Chi-2 test. TE

Table 4 .
Evolution of viral load amongst chidren or adolescents with detectable viral load before initiation of therapeutic education (LHD, Cameroon, February-May 2016), N = 65.
Children and adolescents with undetectable viral load before TE; Group 2: Children and adolescents with detectable viral load before TE; VL: Viral load; n: Number of children or adolescents belong to the category considered of the variable; %: percentage; N: Total number of children or adolescents; OR: Odds Ratio; 95% CI: 95% Confidence Interval; p: Chi-2 test.International Journal of Clinical Medicine 10 sessions of TE remained significantly associated with maintaining an undetectable viral load after initiation of TE (OR = 3.1 (1.1 -8.7 TE: Therapeutic Education; LHD: Laquintinie Hospital of Douala; Group 1: