Results of Combined Chemotherapy and External Beam Radiotherapy for Unilateral Intra-Orbital Retinoblastoma — A Multi-Institutional Study from Mali

Retinoblastoma (RB) treatment aims at saving the life and preserving useful vision. In most low-income countries, because of delays in diagnosis, advanced disease presentation is quite common. This prospective study aimed at evaluating the treatment results of orbital RB with regards to overall survival rate of the patients treated with chemotherapy and radiotherapy. The study was performed from 01 November 2011 to 31 December 2015 in the paediatric oncology unit of Gabriel Touré Teaching Hospital, Bamako, Mali and The Institute of Tropical Ophthalmology of Africa (IOTA), Bamako, Mali. All intra-orbital non-metastatic RB cases not previously treated by chemotherapy or radiotherapy were included in this study. Fourteen patients were included into the study. Median age was 2 years, and sex ratio 2.5 (M = 10; F = 4). Right eye (n = 12, 85.7%) was more often affected than left eye (n = 2; 14.3%). Chemotherapy toxicities were mainly haematological including grade 3 anemia (n = 2; 7%) and grade 4 neutropenia (n = 3; 11%). Twelve patients (86%) were enucleated after neoadjuvant chemotherapy. Two patients (14%) abandoned treatment before enucleation. The IRSS pathology staging was: stage IIIa in 6 patients (50%), and stage II in six patients (50%). Six children (43%) received orbital radiotherapy at total dose of 45 Gy; Six children (43%) achieved complete remission; Overall survival rate was 48% at 4 years (95% How to cite this paper: Togo, B., Traore, F., Diabaté, K., Sylla, F., Tall, A., Simaga, A., Kamaté, B., Diall, H., Togo, P., Doumbia, A.K., Bey, P. and Eshun, F. (2019) Results of Combined Chemotherapy and External Beam Radiotherapy for Unilateral Intra-Orbital Retinoblastoma—A Multi-Institutional Study from Mali. Open Journal of Pediatrics, 9, 39-46. https://doi.org/10.4236/ojped.2019.91005 Received: December 26, 2018 Accepted: January 26, 2019 Published: January 29, 2019 Copyright © 2019 by author(s) and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access


Introduction
Retinoblastoma (RB) is the most common intra ocular malignant tumor of infancy and childhood, and represents 3% of all childhood malignancies.The majority of cases are diagnosed before 2 years of age, and 95% before 5 years.RB is considered a rare tumor in developed countries, and it represents 3% of infant cancers [1].The overall incidence of retinoblastoma is constant worldwide at one case per 15,000 -20,000 live births, but it is higher in developing countries and as a result, it represents the most common pediatric tumor in the developing world [2] [3].Neglected or untreated RB can lead to extraocular dissemination mainly to the optic nerve and sclera (Figure 1).Treatment of RB aims at saving the life and preserving useful vision.In most low-income countries, delays in

Objectives
This prospective study aimed at evaluating the treatment outcomes including overall survival of orbital intraocular non-metastatic RB.

Patients and Methods
The study was performed prospectively from 01 November 2011 to 31 December 2015 (4 years and 2 months duration), in the paediatric oncology unit of Gabriel Touré teaching hospital and in IOTA.
Inclusion criteria were the following: All intra-orbital non-metastatic RB cases, not previously treated by chemotherapy or radiotherapy; patients having no contraindication for study's treatments; patients for whom long-term monitoring is possible and informed consent of parents or legal represent has been obtained.
Geographical representation (urban or rural area) of the patients was documented.At enrolment, all the patients had detailed ophthalmological examination including fundus eye exam under general anaesthesia.Orbito-frontal brain computed tomography (CT scan) and bone marrow and cerebrospinal fluid exam were also performed in all the patients.The international retinoblastoma staging system (IRSS) was used for the classification of patients [7].All the patients underwent a complete clinical oncology evaluation and routine blood check.

Treatment Strategies
Therapeutic guidelines of French-African Paediatric Oncology Group (GFAOP), were utilized Figure 2.

Radiation Therapy
Radiotherapy was done under a Philips 16-bar scan device.For immobilization of the patient, a three-point thermoformed mask was used, aligned with the laser system.General anaesthesia was performed during simulation and during irradiation.Predictive dosimetry was practiced for the choice of energy and ballistics of irradiation beams with the Xio Software.The technic used was the combination of anterior beam and oblique bundles.The dose delivered was 45 Gy in in 1.8 Gy/fraction.

Response Criteria
Complete remission was defined as disappearance of all clinical symptoms and biological signs with medical imaging and ophthalmological exam showing no sign of tumor recurrence.Relapse was defined as any evidence of tumour recurrence regardless of the recurrence site.

Statistical Analysis
Data from medical files of included patients were analysed on IBM SPSS version 20 (SPSS Inc., Chicago, IL).Kaplan-Meier method was used to estimate treated patients' survival, 95% confidence intervals (CI) and log-rank test to compare survival rates in treated patients according to the different forms of Rb [8].Follow-up data for each patient was measured from patient's inclusion in the study up to last data collection.To estimate overall survival (OS), all deaths were considered regardless of causes and survival times of patients who are alive were censored at the date of last contact with the patient up to the date of study closure.The patients who abandoned the treatment were censored at the time of abandonment.

Results
Fourteen patients were included into the study, from 01 November 2011 to 31 December 2015.Patient's characteristics are summarized in Table 1.Median age was 2 years, and sex ratio 2.5 (M = 10; F = 4).

Twenty-eight neoadjuvant chemotherapy courses with Etoposide/Carboplatin
Gy. Six patients did not receive radiation therapy.At the end of treatment and a median follow-up of two years, six children (43%) achieved complete remission.

Discussion
Retinoblastoma is a common ocular tumor in sub-Saharan Africa associated DOI: 10.4236/ojped.2019.9100544 Open Journal of Pediatrics with long diagnostic delays [9].The diagnostic delay in our study was 15 months.Ignorance and poverty of families are contributing factors to this delayed diagnosis.The major reason given by parents for delayed presentation was unawareness about the seriousness of the disease (95%) (Figure 1).Many countries have developed education campaigns with the goal of decreasing diagnostic delay in order to reduce the frequency of advanced disease [10] [11].In our series, the median age at diagnosis was 2 years, with a sex ratio of 2, 5.In Ghana, Essuman et al. found an average age of 25 months at diagnosis [12].Proptosis was the most frequent symptom at admission (n = 10; 75%).
Clinically advanced RB is characterized by proptosis due to orbital extension or optic nerve involvement.The presence of orbital invasion of the intraocular tumor increases the risk of systemic metastasis, associated with poor prognosis [13].Metastases work-up in our patients did not show distant metastases.
CT scan evaluation showed extra-scleral orbital extension in 60% of patients (n = 8) and optic nerve involvement in 40% (n = 6) (Table 1).Other studies in India [14], and in Senegal [15], reported a high percentage of extraocular forms, 37% and 52% respectively.The incidence of orbital RB is still very low in developed countries, due to early diagnosis [16].Treatment of RB requires a multidisciplinary approach including Ophthalmologists, Pediatric Oncologists, Radiation during enucleation [17].The IRSS staging system makes it possible to specify the histological risk factors and stratify patients for adjuvant chemotherapy.However, neoadjuvant chemotherapy affects these risk factors.The pathology staging according IRSS system was as follow: stage IIIa in 6 patients (50%), and stage II in six patients (50%).Pant G, found 63.4% of stage III in a cohort of 41 patients with extraocular RB [18].Soltani et al. in Morocco found the involvement of orbital part of optic nerve in 58.3% and extrascleral involvement in 41.6% [19].
Radiation therapy can treat residual disease after adjuvant chemotherapy.Radiation therapy can lead to growth retardation of the irradiated territory as well as endocrine sequelae due to the irradiation of the pituitary gland.Secondary sarcomas can also occur in the radiation field.Radiation exposure of young children generally increases the risk of developing secondary malignancy.This risk of second cancer is higher when there is germline mutation of the RB1 gene [20].
Patients receive a dose of 45 Gy to the target volume by two electron beams over 5 weeks, with fractions of 1.8 Gy (for children under 12 months old) to 2 Gy (for children over 12 months).Six patients (43%) did not receive radiation because of the long waiting time and unfortunately died of progressive disease.

Conclusion
The therapeutic strategy based on neoadjuvant chemotherapy followed by enucleation, adjuvant chemotherapy and external beam radiotherapy, was beneficial in patients with stage III disease of the IRSS, achieving an event-free survival rate of 48%.
diagnosis result in advanced disease, frequently extraocular.Radiation therapy of retinoblastoma remains controversial as it is fraught with complications especially in very high in infants.Despite these risks, it remains an effective local control measure for the treatment of advanced retinoblastoma.An initial study of retinoblastoma at the unit of pediatric oncology of Bamako showed a survival rate of only 50% [4].In 2011 the global alliance against cancer (AMCC) Curie Institute of Paris (a tertiary referral center in France for retinoblastoma) and the teams of Bamako (Gabriel Toure Hospital and IOTA) developed a program for early diagnosis and treatments, including conservative management and orbital rehabilitation of retinoblastoma in Mali [5] [6].

Figure 1 .
Figure 1.Buphtalmia as a mode of presentation of a unilateral retinoblastoma of the left eye.
oncologists and Radiologists.Most centers use standard doses of carboplatin and etoposide (CE) and cyclophosphamide/vincristine (CO) courses for neoadjuvant and adjuvant chemotherapy of RB.Neoadjuvant chemotherapy should reduce tumoral volume in severely buphthalmic eyes, reducing the risk of eye rupture