A Study of the Relationship between the Taste Sensitivity of Phenylthiocarbamide ( PTC ) and Blood Pressure ( Random Sample from the Students of Qurna College / Basrah-Iraq ) *

The present study aimed to detect the relationship between taste sensitivity of phenylthiocarbamide (PTC) and pressure (systolic and diastolic) among a group of 138 individuals of both sexes (64 males and 74 females), representing a random sample of students and some professors of, aged (20 50) years. The study also included the relationship between eating salted foods and their effect on pressure among males and females of tasters and non-tasters of (PTC) substance. The results showed an increase in the proportion of the phenotypic style of tasters compared to non-tasters among males (75%, 25%) and females (77.03%, 22.97%) respectively. The average of systolic pressure was (117.91 mm/Hg, 107.06 mm/Hg) and diastolic (78.22 mm/Hg, 68.71 mm/Hg) among tasters and on-tasters for both sexes respectively. The results showed significant differences in systolic pressure among non-tasters female compared to their counterpart tasters [X (DF = 1) 5.783, P ≤ 0.05]. Such an effect doesn’t appear among males. The results showed an increase of non-tasters (66.58%) among those with abnormal blood pressure and (68.42%) of those who consumed salted foods.


Introduction
Diseases of blood pressure are complex diseases.They are a result of genetic or *A study of the relationship between the taste sensitivity of phenylthiocarbamide.
How to cite this paper: Mohaus, H.A. and Ayied, A.Y. (2018) A Study of the Relationship between the Taste Sensitivity of Phenylthiocarbamide (PTC) and Blood Pressure (Random Sample from the Students epigenetic and environmental factors (lifestyle, physical activities, food habits), consumption of salty food represents the main environmental factor [1].It was found that consumption of high level of salty food associated with hypertension and an increase in the danger of heart disease and stroke [2] [3] [4] [5].As well as excessive obesity, consumption of high level of fat in the food is another factor to increase the rate of hypertension [5].
Therapeutics with the assistance of food regulation becomes one of the recent features to prevent many diseases especially chronic ones [6].Taste is one of the most important factors to determine the amount and quality of food [7].There is increasing interest day by day in the study of taste and its impact on the nutritional behavior of individuals [8] [9].It was found that the importance of taste is not limited to its role before eating, but found that taste cells have other roles after eating [10].
The ability to taste phenylthiocarbamide PTC (containing N-C=S, which is among the many chemicals that possess the same bitter taste and biological efficacy as 6-n-Propylthiouracil) is an important genetic trait that can play a role in the variation of individuals in the behavior of food to taste sweet and salty substances in addition to a group of vegefoods, fruits and fatty foods [9] [11] [12].
Perhaps the most important is the vegefoods belonging to the Cruciferous family of medical importance in the fight against a number of diseases due to its rich contents in a number of compounds with biological effectiveness and antioxidants [13] [14].
Phenotypically PTC is a simple Mendelian trait under the control of one somatic gene with complete dominance [15] [16].At the molecular level, it was found that the inheritance of this character belongs to the presence of a major gene site controlling the observed variation in the sensitivity of the taste of this substance situated on the seventh chromosome VII (7q34-35) called tas2r38 [17].It is one of the types of cellular receptors to taste bitter substances.The percentage of those who taste this substance is very different among Asian-American, African and European [13] [18] [19] [20].The classification of individuals to tasters and non-tasters is generally associated with their sensitivity of taste for all types of taste: salty, sour and sweet [8] [21] [22].The taste of salt, especially NaCl, is done through epithelial Na channels (EnaC) and these channels in the kidney play an important role in maintaining electrolytes , stability in the whole body [23] [24].Genetic modifications that affect the regulatory mechanism that leads to high blood pressure associated with the sensitivity of tasting salts are still unclear [24].
Understanding the role of genes in regulating blood pressure in the kidney may lead to the development of antihypertensive therapies build on individual's genetic makeup [24].Many factors, including hormones such as insulin, may play a role in regulating taste in general and taste of table salt specifically through sodium ion channels in taste receptor cells (TCR) [25].The individuals were divided into three phenotypes for tasting of PTC substance depending on their We assumed that the non-tasters of PTC who are also less sensitive to the taste of salts especially NaCl may encourage the consumption of more salt which may lead to the possibility of high pressure disease compared to the taster and therefore we seen in present study to detect the accuracy of this conclusion by first, knowing the proportions of the tasters and non-tasters of PTC among a random sample of the students of Qurna Education College/University of Basrah; diastolic and systolic pressure was observed and recorded to detect any differences between them.In addition to measuring the BMI (as a measure of obesity), to determine the extent of eating salted food on blood pressure, we present a questionnaire.Note that this study is conducted for the first time.

Preparation of Gradually Solutions and PTC Sensitivity Test Papers
A 0.13 g of PTC was weighed with a sensitive digital balance, and then was dissolved in 100 ml of distilled water to prepare solution No.1, which represents the highest concentration, and attended a series of gradual dilution [16].A boiling distill water was considered as control.Ashly [29] method to prepare PTC test paper was followed (Table 1).

PTC Sensitivity Test
Individuals were asked to wash their mouths with fresh water to avoid any bitter or other taste.Then, they started to taste filter paper (control) followed by PTC test paper with grade 13 (lowest PTC concentration) towards the highest concentration (sol.No.1 PTC concentration) till they feel the bitter taste when the degree of PTC sensitive test was recorded, each person repeated this practice three times.The grade recorded when each person had the same grade twice or more times.

Critical PTC Test Value (Threshold) and Tasting of Salting Foods
PTC critical test value was recorded for each individual.Phenotypic classes of PTC test trait were determined depending on critical binomial distribution.The anti-mode point was used to the classified individual as tasters and non-tasters [30].In the case of eating salted foods, the questionnaire method was used to identify them.

Biometric Measurements
Systolic (SBP) and diastolic (DBP) pressures were measured for each individual using the mercury scale.High blood pressure was determined as 140 mmHg SBP or higher or diastolic pressure (DBP) 90 mmHg or higher [31].More or less than normal value were consider as an abnormal of blood pressure The weight, length of each person were measured and the biomass index (BMI) was calculated as weight, kg/height, m 2 .

Statistical Analysis
The frequency of each PTC sensitive phenotypic and genotypic classes was calculated as a percent of each class to the total number of tested individuals.
Chi-square test was used to determine significant differences between different classes and groups.0.05 was assigned as a significant level.Statistical packages IBM SPSS (version 22, 2013) was used for all statistical analysis.

Results
PTC taste threshold No. 3 represented the anti-mode of the binomial distribution of PTC taster and non-taster curve (Figure 1).
Table 2 shows the mean of SBP, DBP, and BMI of males' taster and non-taster.
Tasters and non-tasters percentages were 75% and 25% respectively.Mean of SBP and DBP among males tasters were 117.91 and 78.22 mm/Hg respectively, those for non-tasters were 107.06 and 68.71 mm/Hg respectively.Mean of BMI of male tasters and non-tasters were 24.70 and 26.86 kg/m 2 respectively.Statistical analysis did not reveal significant differences between males' tasters and non-tasters for both blood pressure and BMI index.
In the case of females, the distribution of DBP appeared to be different between Journal of Biosciences and Medicines  tasters and non-tasters Table 3.It was noticed a significant differences between the two groups [X 2 (DF = 1) = 5.78, P ≤ 0.05].The value of BMI between females' tasters and non-tasters were nearly similar 22.84 and 22.801 kg/m 2 respectively.
There was a significant (P < 0.05) association between individual consumed salty food with abnormal blood pressure, especially in males (Table 6).Individuals with abnormal blood pressure and consumed salty food recorded 68.42%, other don't consume salty foods 31.58%from the total number of abnormal blood pressure individuals (total number was 19).

Discussion
Our results indicate that there is a high percentage of people with abnormal blood pressures among those who are not tasters and those prefer salted foods, which are more likely to be infected The results also indicate that there is a gender impact in the incidence of abnormal blood pressures, as it rises among   non-tasters women more than males .The BMI scale did not have a role in the studied sample.This may be due to the fact that the sample studied is mostly young.Hypertension is a major cause of heart attack, which is the leading cause of death in the world, according to the World Health Organization [31] [32].
The variation in taste at the individual, ethnic or groups' level may play a role in the variability of the diseases [33] [34] [35].The taste is influenced by many factors, perhaps the most important age and sex, as women are more accurate in Journal of Biosciences and Medicines the taste of men [26] [36] [37].It has been found that non-tasters of PTC is more confused in the accuracy of taste compared to the tasters and this may lead to the infection of some diseases at sometimes [38].Also, both alleles of the taster or non-taster of the PTC can contribute to the infection and the resistance of some diseases being the non-taster are the most susceptible [39] [40].Timpson et al. [21] pointed to the possibility of non-taster infected by heart disease and stroke more than tasters, but the results did not confirm this.
Bitter substances play an important role in human life as a medicine in addition to its role as food [14].These substances and their receptors can also play a role in food digestion and metabolism [41] and homeostasis of the human body [11] [42] [43].The presence of taste receptors for bitter substances in extra oral cavity suggest that they may play important and varied roles in the rest of the body depending on where they are located; as in the respiratory tract [44] [45], along gastrointestinal [46] [47], cardiac tissue [48], thyroid [49], blood cell [50] and the lung [51].The genetic expression of the bitter taste receptors in renal epithelial cells indicates that these receptors may play roles in evolution and overall functions of kidney [52].
High blood pressure is one of the most complicated diseases and there are many common causes of this disease.Eating or consuming salts is a major cause, in addition to many other internal factors, obesity is one of the main causes of high blood pressure [53].Research results varied in relation to BMI and PTC taste, some support and others denied [34] [54] [55] PTC non tasters women may have a different mechanism of Weight regulation [56].
The genetic manipulation that affects the regulatory mechanism leads to high blood pressure associated with the sensitivity of salty taste are still unclear [24].
The discovery of the presence of the bitter taste receptors in the epithelial cells of the kidney [52] is a clear indication of the comprehensiveness and diversity of its functions in the regulation of many activities of the body, including the kidney and its functions.The most important of which is the regulation of ionic saline balance.The individuals variation of genetically modified in the forms of these receptors may be reflected negatively or positively in maintaining this balance.
Many researchers have suggested that there may be physiological differences between tasters and non-tasters, as hormones levels [23] [57].Hormones also play a role in regulating pressure [23] [25].The life of the patient with hypertension depends mainly on the quality of the food he is eating and that the sudden rise (specifically of salts) or the sudden drop causes a heart stroke, as the increase in salt intake or lack of excretion leads to a rise in the bloodstream, and the average reduction of salt can significantly reduce stress in healthy or high blood pressure people [23] [25] [58] [59] and thus heart attack [60].
The importance of taste in human life is illustrated by the effect of taste on its behavior in the choice of food and hence on its dietary habits and the identification of food or toxins [61] [62].The variation in the taste ratio of PTC is associated with the variation in food selection and is probably associated with food-related diseases among modern human societies [13] [63].PTC tasting polymorphism could be a useful biomarker in health and disease [9] [47] [64].As indicated by many recent researches, which proved that eating a variety of fresh foods from fruits and vegetables can be sufficient to treat many chronic diseases [65].Therefore, the regulation of food and lifestyle may have an effect in the treatment of hypertension [66], so this trait could be one of these strategies.

Conclusion
People that suffer from high blood pressure were mostly non-tasters, 70% in males and 63.15% in females.As well as non-tasters, females as a whole showed higher DBP than taster females.There is association between salty food and blood pressure in males only.

Table 1 .
Concentration of taste testing series of PTC solutions used in the present study.

Table 2 .
Mean of SBP, DBP, and BMI of males' taster and non-tasters.
SBP: Systolic Blood Pressure, DBP: Diastolic Blood Pressure, BMI: Body Mass Index, a Means of each trait with same subscripts are not significantly differing.

Table 3 .
Mean of SBP, DBP, and BMI of females' tasters and non-tasters.

Table 4 .
Mean of SBP, DBP, and BMI of males and females tasters and non-tasters.
BP: Systolic Blood Pressure, DBP: Diastolic Blood Pressure, BMI: Body Mass Index, a Means of each trait with same subscripts are not significantly differing.

Table 5 .
Number and percent of tasters and non-tasters with abnormal blood pressure.

Table 6 .
Association between salty food consumption and abnormal blood pressure (males and females).
a,b Percentages with different subscripts each columns different significantly at 0.05.