Meta-Analysis of Ventilated versus Spontaneously Breathing Patients in Predicting Fluid Responsiveness by Inferior Vena Cava Variation

Purpose: Respiratory variation in inferior vena cava (ΔIVC) has been extensively studied in predicting fluid responsiveness, but the results are conflicting. We performed a systemic review and meta-analysis of studies aiming at investigating the diagnostic accuracy of ΔIVC in predicting fluid responsiveness. Methods: MEDLINE, EMBASE, Cochrane Database and Web of Science were screened for relevant original and review articles from inception to July 2016. The meta-analysis determined the pooled sensitivity, specificity, diagnostic odds ratio (DOR) and area under the ROC curve (AUROC). In addition, subgroup analyses were performed in mechanically ventilated patients and spontaneously breathing patients. Results: A total of 20 studies involving 635 patients were included. Cutoff values of ΔIVC varied from 12% to 42%, the pooled sensitivity and specificity was 0.68 (0.62 0.75) and 0.80 (0.75 0.85), respectively. The DOR was 14.2 (6.0 33.6) and the AUROC was 0.86 (0.78 0.93). Subgroup analysis showed better diagnostic performance in patients on mechanical ventilation than in spontaneously breathing patients with higher sensitivity (0.75 vs. 0.56), specificity (0.82 vs. 0.78), DOR (22.9 vs. 7.9) and AUROC (0.90 vs. 0.80). The best threshold of ΔIVC in patients on mechanical ventilation was IVC distensibility index (ΔdIVC ≥ 17% ± 4%), compared to IVC collapsibility index (ΔcIVC ≥ 33% ± 12%) in spontaneously breathing patients. Conclusion: ΔIVC is not an accurate predictor of fluid responsiveness in patients with acute circulatory failure. In patients on mechanical ventilation, the predicting ability of ΔIVC was moderate with acceptable sensitivity and specificity; in spontaneously breathing patients, the specificity remains acceptable but its sensitivity is poor. How to cite this paper: Si, X., Cao, D.Y., Xu, H.L. and Guan, X.D. (2018) Meta-Analysis of Ventilated versus Spontaneously Breathing Patients in Predicting Fluid Responsiveness by Inferior Vena Cava Variation. International Journal of Clinical Medicine, 9, 760-777. https://doi.org/10.4236/ijcm.2018.910063 Received: October 10, 2018 Accepted: October 23, 2018 Published: October 26, 2018 Copyright © 2018 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access


Introduction
Hypovolemia is a very frequent clinical situation in the intensive care unit (ICU) and is primarily treated with volume expansion (VE).The only goal of VE is to improve the cardiac output (CO) of the patients especially those with acute circulatory failure [1].However, multiple studies have demonstrated that only approximately 50% of hemodynamically unstable patients respond to VE in the ICU [2].It is therefore essential to have reliable tools to predict the efficacy of VE and ultimately distinguish patients who may benefit from VE from those who are unlikely to respond.Recently, many studies have focused on the prediction of fluid responsiveness.Static hemodynamic indices have been of little value in predicting fluid responsiveness [3] [4].In contrast, dynamic indices, based on analysis of preload dependence, have been validated as factors that can help predict fluid responsiveness [3] [5] [6] [7].However, because of invasiveness and high cost, the application of these indices is of limited use in emergency rooms and general wards.
Bedside point-of-care ultrasonography has gained considerable attention because of noninvasiveness, rapid diagnosis and low cost [8].The diameter of the inferior vena cava (IVC) is easily recorded by transthoracic echocardiography (TTE) in a subcostal view.Because of the heart-lung interactions, the maximum IVC diameter (IVCmax) and minimum IVC diameter (IVCmin) can be measured during thecycle of breath.Then, a term named respiratory variation in IVC diameter (ΔIVC) can be calculated.In recent years, intensivist had increasing interesting on ΔIVC for predicting fluid responsiveness.
Following the first study demonstrating the accuracy of the ΔIVC, it has been extensively investigated for its usefulness.In 2014, a meta-analysis pooling eight studies published at that time confirmed that ΔIVC is of great value in predicting fluid responsiveness [9].However, since this meta-analysis, conflicting findings on its accuracy have been reported in a number of publications.
In order to clarify these mixed results and assess the ability of ΔIVC to predict fluid responsiveness, we conducted a systemic review of all these studies and performed a meta-analysis, with hypothesis that ΔIVC performs well in predicting fluid responsiveness.

Clinical Research Question
The clinical research question was: What is the sensitivity and specificity of the ΔIVC when using it to predict fluid responsiveness?X. Si

PICO Statement [10]
The PICO statement is as the following: P-patient, problem or population: patients with acute circulatory failure in whom the effect of volume expansion (VE) is unknown and needs to be predicted.
I-intervention: Inferior vena cava (IVC) diameter was examined subcostally and measured in M-mode or 2D mode, 2 cm before the IVC joined the right atrium.The IVC respiratory variation (ΔIVC) was calculate by recordingthe largest and smallest IVCdiameter at end-inspiration or end-expiration.
C-comparison, control, and comparator: Fluid responsiveness was defined as a significant increase of stroke volume (SV), cardiac output (CO) or other surrogates during a VE.
O-outcomes: Ability of the △IVC to predict fluid responsiveness.

Searching Strategy, Study Identification and Data Extraction
Our aim was to identify all studies evaluating the ability of the ΔIVC to predict fluid responsiveness compared to the increase in SV, CO or other surrogates induced by subsequent VE.
We searched the MEDLINE, EMBASE, Cochrane and Web of Science databases for relative studies published in English from inception to July 2016.The key words we used consist of term related to IVC ("inferior vena cava", "caval index", "collapsibility" and "distensibility") and terms related to volume status ("fluid or volume or preload responsiveness", "fluid or preload challenge", "preload dependence or independence or dependency or independency", "functional haemodynamic monitoring" and "fluid therapy or management").These key words were searched separately by two groups using different combination strategy.We also looked for relevant articles cited in review articles, commentaries and editorials.The search was performed repeatedly until no new studies could be found.
Study identification was performed in two steps.
Step 1 comprised screening for titles and abstracts, and step 2, review of full texts of studies obtained in step 1.We only included studies investigating the accuracy of the △IVC that were published in full text or accepted for publication in indexed journals.Excluded criteria were 1) studies using central venous pressure or right atrial pressure as the reference standard, because these static parameters cannot predict fluid responsiveness accurately; 2) studies measuring IVC with techniques other than ultrasonography; 3) studies involving animals and healthy volunteers.Two reviewers process searching independently, disagreement was settled by a third opinion.The quality of the included studies was evaluated by using the QUADAS-2 scale [11].The meta-analysis was performed according to the PRISMA statement.
Important information was extracted from the included articles using a standardized data form by two reviewers.Extracted data include the name of the first

QUADAS-2 Quality Assessment in Included Studies
Included studies were assessed for their quality based on the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) protocol.QUADAS-2 scale [11] was made up of 4 domains: patient selection, index test, reference standard, and flow and timing.Each domain is assessed in terms of risk of bias, and the first 3 domains are also assessed in terms of concerns regarding applicability.For the ''patient selection'' domain, we examined whether patients were consecutively included and whether inappropriate exclusions were avoided.For the ''index test'' domain, we examined whether the threshold used to define volume responsiveness was pre-specified.For the ''reference standard'' domain, we examined whether the result of VE on SV, CO or surrogates was assessed without knowledge of ΔIVC result.Finally, for the ''flow and timing'' domain, the authors examined whether there was an appropriate interval between IVC measurement and VE, whether patients received the same VE and whether all patients were included in the analysis.For each criterion, the risk was judged as high, low and unclear.

Statistical Analysis
We performed a meta-analysis in order to determine the pool sensitivity, specificity and diagnostic odds ratio (DOR).In addition, the pooled area under the ROC curve (AUROC) and threshold for ΔIVC as a predictor of fluid responsiveness was also evaluated.To investigate a threshold effect, we calculated the Spearman correlation coefficient between sensitivity and specificity.Homogeneity between studies was tested by the Chi squared test and I 2 index.According to heterogeneity, we adopted a random effect model by using the method of Der-Simonian-Laird from the Mantel-Haenszel model.We compared studies with ICU setting versus non-ICU setting making the hypothesis that ΔIVC could be more reliable in ICU patients.We compared studies with adults versus children making the hypothesis that ΔIVC could be more reliable in adults.We compared studies with different devices for measuring IVC making the hypothesis that one device is better than the others.We compared studies with three different formulas for the calculation of ΔIVC making the hypothesis that one formula is better than the others.We compared studies with patients on mechanical ventilation versus studies with spontaneously breathing patients, testing the hypothesis that the reliability of ΔIVC is better in patients on mechanical ventilation.We compared studies where fluid responsiveness was defined by an increase in SV, International Journal of Clinical Medicine CO or surrogate ≥ 15% versus studies with other definitions of fluid responsiveness, testing the hypothesis that the reliability of ΔIVC is better when fluid responsiveness is defined by a larger increase.We compared studies where SV, CO or surrogate were measured by echocardiography versus studies where they were measured by other methods, testing the hypothesis that the reliability of ΔIVC is better when SV, CO or surrogate were measured by echocardiography.Finally, we compared studies where VE was performed with versus studies where it was performed by colloids, testing the hypothesis that the reliability of ΔIVC is better when VE is performed with colloids.Causes of heterogeneity were also investigated by meta-regression based on the Littenberg and Mose linear model.
Results are expressed as mean (95% confidence interval) or as mean ± standard deviation.The meta analysis was performed with Meta-Disc v.1.4(Universidad Complutense, Madrid, Spain).The additional statistical analysis was performed with MedCal 15.2.2 (MedCal Software, Mariakerke, Belgium).A two-tailed p < 0.05 was considered to statistical significance.

Characteristics of Included Studies
A flow chart of the study selection is provided in Figure 1.Our initial search identified 399 citations.379 of them were excluded: 320 for not relating to the subject, 49 for being reviews, letters, guidelines, case reports and editorials, 3 for not writing in English, 4 for not using proper reference standard, 3 for being animal experiments.Finally, a total of 20 studies [12]- [31] reported the ability of △IVC to predict fluid responsiveness were included in our analysis.
Characteristics of included studies are listed in Table

Prediction of Fluid Responsiveness by ΔIVC
The diagnostic performance of ΔIVC in each study is showed in Table 2.The

Discussion
This meta-analysis including 20 studies with a combined total of 635 patients concluded that ICU staff must be cautious of using ΔIVC, which was not so excellent to predict fluid responsiveness with pooled sensitivity (0.68) and specificity (0.80).In patients on mechanical ventilation, ΔIVC could predict fluid responsiveness moderately with acceptable pooled sensitivity (0.75) and specificity (0.82).The pooled AUROC was 0.90 (0.80 -0.99) and the average of threshold was ΔIVC ≥ 17% ± 4%.However, in spontaneously breathing patients, ΔIVC predict fluid responsiveness with poor sensitivity (0.56) and acceptable specificity (0.78).
Point-of-care ultrasonography is a reliable monitoring technique and is becoming increasingly popular in the ICU.The IVC diameter is easily examined from a subcostal view in a longitudinal section, varying during the respiratory cycle due to the changes in intrathoracic pressure during inspiration and expiration.This variation is expressed as the △IVC.Recent years, ΔVC has been developed to Based on the results from a large number of patients, we found that ΔIVC was not so excellent to predict fluid responsiveness with poor sensitivity (0.68) and acceptable specificity (0.80).The pooled AUROC was 0.86 but not close to each other.In addition, the threshold values for ΔIVC varied across studies, ranging from 12% to 42%, which reinforce our conclusion.
In subgroup analysis, our study indicated that in patients on mechanical ventilation, ΔIVC predict fluid responsiveness with acceptable pooled sensitivity (0.75) and specificity (0.82), which are less accurate than meta-analysis performed by Zhang et al., however.This is likely due to high PEEP and/or low tidal volume invalidating the diagnostic performance of ΔIVC.High PEEP has been demonstrated to elevate right atrial pressure (RAP) and IVC pressure, while simultaneously reducing venous return, introducing an increase IVC size and false negative of ΔIVC [34].Furthermore, the low tidal volumes less than 8 ml/kg will cause smaller variations in intrathoratic blood volume, resulting in smaller ΔIVC theoretically, irrespective of volume status.Charbonneau et al. [23] [35] shows deeper the breathing is, the larger diaphragmatic motion and ΔIVC are, regardless of volume status.This indicates that shallow breaths may minify ΔIVC and reduce its sensitivity, while inspiratory efforts may magnify ΔIVC and reduce its specificity [18].Even if in patients on ventilation, ΔIVC is not a valid measure when patients made an inspiratory effort [36].
An important point that must be paid more attention to is the formula of calculation of ΔIVC.ΔIVC is usually expressed as the difference between expiratory IVC diameter and inspiratory IVC diameter divided by the expiratory IVC diameter, multiplied by 100%.However, in spontaneous respiration or mechanical ventilation, the changes of IVC diameter are opposite because of opposite changes of intrathoracic pressure during inspiration.In patients on mechanical ventilation, ΔIVC is calculated by (IVCmax − IVCmin)/IVCmin defined as IVC distensibility index (ΔdIVC), while in spontaneously breathing patients, it is calculated by (IVCmax − IVCmin)/IVCmax defined as IVC collapsibility index (ΔcIVC).In our meta-analysis, the best threshold of ΔIVC in patients on mechanical ventilation was ΔdIVC ≥ 17% ± 4%, compared to ΔcIVC ≥ 33% ± 12% in spontaneously breathing patients.Nowadays, the clinical use of ΔIVC is in chaos regardless of its physiology, leading to misjudgment, which need to be more accurate define and recognition.
There are some limitations that should be noted for interpreting the results.
First, the heterogeneity of the included studies existed with respect to patient population, respiratory pattern, calculation formula, definition of index test and fluid responsiveness.Nevertheless, no threshold effect was detected.Furthermore, both the subgroup analyses and meta-regression were opposed to the influence of heterogeneity on the results.Second, although we performed subgroup analysis, the number of studies and sample size in each subgroup was small, the conclusion needs to be validated in future trials.Third, we did not include studies not in English, non-full-text and unpublished studies, which may increase the risk of reporting bias.

Figure 3 .
Figure 3. Pooled diagnostic accuracy of ΔIVC in whole studies.

Figure 4 .
Figure 4. Summary receiver operating characteristics curve of ΔIVC in whole studies.
et al.

Table 1 .
Characteristics of the studies included.

Table 2 .
Sensitivity and specificity of ΔIVC in predicting fluid responsiveness.

Table 3 .
Pooled diagnostic accuracy of ΔIVC in whole and subgroup studies.
sug-X.Si et al.DOI: 10.4236/ijcm.2018.910063773 International Journal of Clinical Medicine gested that ΔIVC predicted fluid responsiveness with low sensitivity (38%), and Baker et al. [21] demonstrated that ΔIVC was an inaccuracy predictor with low AUROC (0.46).The ventilation of these two studies was High PEEP > 5 cm H 2 O and low tidal volumes < 8 ml/kg.However, these two studies [21] [23] were published after the meta-analysis performed by Zhang et al.In addition, our study . Si et al.DOI: 10.4236/ijcm.2018.910063774 International Journal of Clinical Medicine dicting ability of ΔIVC was moderate in patients on mechanical ventilation, while it was poor in spontaneously breathing patients.Thus, intensivist must be cautious of using ΔIVC.