Telomeres and Telomerase: Molecular Views and Perspectives

Telomere, the nucleoprotein structure at the end of eukaryotic linear chromosomes is indispensable for maintaining the genome stability. Telomeric DNA loss is apparent with each cell division, which marks an endpoint to the indefinite replication of the cell by causing replicative senescence that may lead to the programmed cell death. The loss of telomere is normal in cell division and as such after 20 40 divisions, telomere becomes too short to facilitate the capping function. Telomere uncapping or chromosomal free end causes a potential threat to the genomic stability and thus leads to the accumulation of chromosomal abnormalities that have been known to play a role in aging and cancer. Telomerase, the ribonucleoprotein complex, and its accessory proteins are required to maintain the telomere sequence. Telomerase plays a key role in maintaining the length of telomere by adding G-rich repeat sequences. Its activity has been found to be quite high in the gametes, stem cells and most importantly tumor cells. Almost 85% of tumor cells compensate for telomere loss aided by telomerase-associated protein complex and shelter in complex or telosome. However, 5% 10% of the cells undergo telomerase-independent mechanism. This review presents the molecular view of the telomere and telomerase along with its associated complex structures. It also discusses its contrasting role in causing cellular senescence and promoting tumorigenesis.


Introduction
Telomeres are chromatin structures that cap and protect the end of chromosomes.It is the end of eukaryote linear chromosomes, consisting of tandem ar-DOI: 10.4236/aar.2018.75008 92 Advances in Aging Research rays of G-rich repeats that protect the genome from degradation.A major part of the vertebrate telomere is packaged in closely spaced nucleosomes [1].In mammals, telomeres comprise of thousands of tandem repeats of oligonucleotide sequence TTAGGG, associated with various specific proteins.Telomeric repeat sequences vary considerably between and within species [2].In the case of humans, the length of telomeric repeat sequences has been reported up to 15 kb [3].Telomere plays an indispensable role in protecting the chromosomal ends from fusion and degradation.During cell division, telomere compensates for DNA loss and consequently for telomere shortening [4].
Telomere plays a crucial role in aging.In human somatic cells, shortening of telomeres occurs with each cell division [5].Since DNA polymerases are unable to completely replicate linear chromosomes, telomeres lose telomeric repeats progressively [2].Progressive telomere shortening affects the chromosomal cap, which further leads to chromosomal instability, end-to-end fusion and ultimately cell death [1] [6].The end-to-end fusion of chromosomes due to gradual telomere shortening may lead to the formation of dicentric chromosomes, which mark a potential threat to cellular viability.Chromosomal instability caused due to telomere loss is directly linked with apoptosis (Programmed cell death) as illustrated in Figure 1.Normally, the telomere is eroded after each cell division that results in the loss of about 50 -200 bps.Gradually, due to the telomeric erosion, telomeric length becomes too short for the chromosome to be capped and provides a platform for nucleoprotein complex formation.Further, the chromosome is no longer able to bind shelterin or associated proteins, and thus telomere uncapping occurs.The uncapped telomere affects the genome integrity and paves the way for the cell towards programmed cell death [7].According to Hayflick (1998), cell division stops after a certain number of cycles after which they enter into a stage of irreversible cell arrest or senescence [8].Senescence of the cells is triggered through telomere signaling pathway, which involves p53 and RB protein factor [9].Studies carried out on p53 and RB-deficient primary cell lines demonstrate the occurrence of a crisis in those cells, a period when such cells continue to grow, divide and cross the Hayflick's limit and end up with extreme shortening of telomere which further leads to genetic instability and finally massive apoptosis occurs [10].Thus, erosion of telomere is one of the important mechanisms which limit the proliferative potential of individual cells [8] [11].
The gradual loss of telomeric repeats at the chromosomal ends affects cell function during aging.Typically, human chromosome ends have 2 -10 kilobase pairs of detectable telomere repeats depending on the type of tissue, the age of the donor and the replicative history of the cells.Human chromosomal ends show marked variation in the average length of telomeres, for example, chromosome 17p has one of the shortest arrays of repeats [2].The dramatic effect of the telomere loss is often conspicuous in cancer cells wherein gradual telomere loss is considered as DSBs (Double-stranded breaks).This situation results from the loss of tumor suppressor alleles and leads the cells towards a state of immortality [12].Studies done on vascular endothelium cells showed an inverse relationship between telomeric length and age [13].For human peripheral blood cells, telomere length appears to be a predictive marker of age-dependent mortality [14].In order to compensate the telomere loss, telomerase and a complex network of telomere-associated proteins are needed.One remarkable feature of nucleoprotein structure located at the end of the chromosomes is to protect the chromosomal ends and prevent them from being considered as double-stranded breaks (DSBs).

Telomere-Associated Structures
Telomere protects the chromosomal ends by forming various higher order structures.The telomere-associated high order structures pose serious challenges for the maintenance of fidelity during DNA replication, repair and recombination which may cause potential threats to telomere stability [3].Telomere capping at the end of the chromosome helps to avoid unwanted end-to-end fusion and thus maintains cellular viability.T-loop is one of the highest order complex structures that telomeres attain to perform a remarkable function of protecting the chromosome by sequestering its free end Figure 2(a).Being sequestered, the chromosomal free ends are not recognized as DNA breaks and thus the occurrence of unwanted DNA damage responses get restricted [7].A multisubunit protein complex called shelterin (also called as telosome) promotes formation of T-loop.Shelterin is a six protein subunit complex and includes TRF1 and TRF2 (telomeric repeat binding factors), POT1 (protection of telomeres), RAP1 (transcription repressor/activator protein 1), TIN2 (TRF1 interacting protein 2) and TPP1 (POT1 and TIN2-organizing protein) [16].TRF1 and TRF2 are telomere-associated dimers reported to restrict telomere elongation by telomerase and thus termed as negative regulators of telomeric length [16] [17].Shelterin binds specifically to telomeric DNA and provides a protective capping to the chromosomal end.Telomeric repeat sequences prepare a ground for the specificity of Shelterin towards telomeric DNA.TRF1, TRF2 and POT1 are the first three components of Shelterin to identify TTAGGG repeat sequences and bind specifically to it.TRF1 and TRF2 bind to the double-stranded telomeric DNA whereas POT1 binds to single stranded 3' overhang as well as the D-loop [16].D-loop is formed as a result of G-rich 3'overhang strand invasion and followed by its hybridization with proximally lying c-rich complementary sequences [17].Studies done so far reveal quite contrasting features of the Shelterin complex, TRF1 and TRF2.
Of the components of shelterin complex, TRF1 and TRF2 share some common  (2002) in an in vitro study demonstrated the affinity of p53 towards the 3'overhang region which was not sequence specific [24].Most interestingly, it was also observed that the efficiency of T-loop formation doubled when catalyzed by TRF2 in the presence of p53, which clearly depicts the unique role of p53 in T-loop formation [24].However, the detailed molecular mechanism associated with T-loop, its dynamics and the way it persists throughout the cell cycle is not yet clear.
The detailed formation of Shelterin protein complex is illustrated in Figure 2(b).Apart from shelterin complex, mammalian telomeres have also been In human, single-stranded telomere with 3' G-overhang causes the formation of other higher order structure G-quadruplexes [26].The G-quadruplexes are highly stable secondary coplanar structures and forms anywhere along the long G-rich strand.The coplanar structure of G-quadruplexes involves the presence of four guanine bases interconnected with the pairs of hoogsteen hydrogen bonds and stacked with each other to form G-tetrad structure [18].The presence of electronegative O-6 guanine carbonyl group towards the center of each tetrad provides the site for the interaction of small ions like Na + ions or K + ions which invokes high stability to the G-quadruplex structures [27].In vitro study on Oxytrich nova's telomerase suggests that the stability of the structure is inferred by the binding of K + ion [28].K + ion coordinates between the adjacent strands and "G-bases" in G-quadruplexes, serves as both donor and acceptor for hydrogen bond formation and thus G-quadruplexes includes intra-or intermolecular interaction of G-rich single-stranded telomeric ends.In humans, the functional roles of G-quadruplexes include telomere protection, suppression of recombination, gene expression, and inhibition of telomerase-dependent telomere extension and telomere maintenance [29] [30].Formation of the G-quadruplex structure causes the unavailability of free 3' telomeric end region to bind with the RNA template of telomerase and thus hinders the catalytic activity of the enzyme complex [28].The G-quadruplex formation is also one of the several potential obstacles telomeres pose to DNA during replication.Therefore, unfolding of G-quadruplex is required for the attachment of telomerase and executing its catalytic activity [31] [32].Thus, it could be said that stabilization of G-quadruplex inhibits the action of telomerase.The factors like small molecules, which favor the stability, prove to be a selective target of immortalized cells wherein active telomerase is found to be quite high.The concerned approach has led to widespread development of anti-cancer therapeutic agents viz., BRACO-19, RHPS4, telomestatin and tetra substituted naphthalenediamide which are proved to be effective candidates against cancer cells by altering the telomere maintenance [33] [34] [35] [36].A schematic model representing the formation of G-quadruplex is shown in Figure 3.

Telomerase-A Molecular Insight
Human telomerase is a ribonucleoprotein holoenzyme complex which functions to extend telomeric DNA and acts to compensate telomere loss [6].Telomerase comprises of a catalytic protein subunit which belongs to the family of reverse  The telomere is thought to be an internal clock which terminates a cell's life [41].Telomerase compensates the loss of telomere end sequences during cell division by adding specific DNA repeats at the chromosomal 3' ends [42].It synthesizes and maintains telomeric units to counter the end replication problem by adding repeated oligomer sequence to the 3' terminus of chromosomal DNA [43].The process of telomere elongation involves various stages as represented in Figure 5.The template sequence of 11 complementary nucleotides located inside the RNA domain of the telomerase ribonucleoprotein complex gets hybridized with the complementary two telomeric repeats found at the 3' extreme of Normally, telomerase activity is observed in those cell types that replicate successfully and most importantly those which exhibits regenerative activity, such as sperm cells [44], stem cells [45] and activated lymphocytes [46].During S-phase in most somatic cells, some telomerase activity is transiently expressed [47] and is critical for cell cycle transit and for preventing or delaying cellular senescence.Telomerase supports the replicative potential of proliferating cells.
Telomeric length is frequently being used a marker of somatic cells replicative history.Several studies done so far suggest that telomeric length can be used as surrogate marker for various other tissues based on the investigated measurement of telomere length in white blood cells [48].In a recent study, it has been shown that two core components of telomerase viz., hTERT and hTERC undergo a gradual reduction in expression with increased age [49].As far as the con-

TERC and TERT Genes
hTERT gene is located on chromosome 5p15.33and encodes a protein called hTERT which consists of 1,132 amino acids (Human Reverse Transcriptase) and forms the catalytic subunit of the telomerase.hTERC gene that is located on chromosome 3q21-q28, encodes telomerase RNA and consists of 451 nucleotides [51].It serves as an RNA template that associates with the Reverse transcriptase family and forms telomerase ribonu cleoprotein complex [52].Human hTERT has been found to express only in telomerase-positive cells [44] [53].In human, hTERC has been shown to be ubiquitously expressed in germ cell lines and stem cells [37].Moreover, it has been reported that in most human tumors, chromosomal gains and gene amplification involving chromosome arm 3q (TERT and TERC telomerase subunit genes) has been found to be more frequent [54] [55].
Studies suggest that mutations in hTERC gene give rise to an array of human diseases, for instance, autosomal dominant dyskeratosis congenital disease in humans [56].Chromosomal instability and accelerated cellular senescence have been investigated in patients affected by dyskeratosis congenital and idiopathic pulmonary fibrosis, especially in tissues with a high proliferative activity [57].

hTERT and hTERC-Components of Telomerase
Telomerase activity requires catalytic subunit hTERT and the RNA subunit hTERC for reverse transcription [58] [59].Studies done so far to understand the structure and function of TERT reveal that homologs of TERT share a common domain structure.The catalytic subunit of the TERT (127 kda), is found in the C-terminal region of the protein [16].TERT, like other polymerases, contains a triad of absolutely conserved aspartic acid (Asp) residues and the conserved aspartic acid residue is required for metal binding and enzyme chemistry [59].
The human telomerase reverse transcriptase is a catalytically active component of the telomerase complex and plays a crucial role in catalyzing the telomerase elongation process, genome stability and enhanced DNA-repair [60] [61].
hTERT in association with an RNA template molecule (hTERC) and a number of telomerase-associated proteins form the active enzyme complex and execute its canonical function of telomerase protection [37] [62] [63] [64].Studies suggest that there is a steady decrease in the expression level of hTERC and hTERT gene during the human gestational age of 6 -11 weeks [65].
The activity of the telomerase depends particularly on hTERT, the catalytic component of telomerase and is considered to be the rate-limiting component of telomerase activity [66] [67] [68].Studies done on mice and cultured cells, in the absence of telomerase enzymatic activity, revealed novel functions of telomerase and its functional subunit TERT apart from its canonical role in telomere maintenance.Telomerase in association with TERT has been found to execute some telomere-independent activity.Telomere-independent activity of telomerase in-cludes enhancement of cellular proliferation, providing resistance to cellular senescence [69].It has also a potential role in DNA repair mechanisms [70], signal amplification through wnt pathway [71], RNA-dependent RNA polymerase function [72].Moreover, TERT also plays a crucial role in mitochondrial ROS (Reactive Oxygen Species) production and takes an active part in mitochondrial metabolism as well [73].
Experimental studies suggest an active role of TERT in cancer cells.Since telomere maintenance is one of the key events in cancer, it causes activation of the hTERT gene by chromatin remodeling [74] [75].Reste et al. (2014) implicates in their study that in order to initiate the upregulation of telomerase gene, the combined contribution of several conditions viz., a specific mixture of regulating cytokines and a certain confirmation of telomeric ends is required simultaneously [41].Upregulation of telomerase activity is partly independent of hTERT mRNA expression as in some studies the later was demonstrated to remain unaffected [76] [77].It has been proposed that excess of TERC gene dose is detrimental and can play a role in promoting cancer [54] [78] [79].TERT activity has been extensively studied during the last decade but many questions still remain unanswered.Activation of the hTERT gene regulation is still unclear.

Telomerase: Path towards Tumorigenesis
After each division of somatic cells, telomere shortening occurs due to the ina- [85].Such immortalized cells maintain the length of telomere without the aid of telomerase and are found to be more common in tumors derived from mesenchymal cells [81].ALT cells have been characterized with long and heterogeneous telomeres (2 -20 kb), long and extrachromosomal telomeric repeats and the presence of ALT-associated promyolytic leukemia bodies (ABPs) [86].
Telomerase instability paves a molecular path towards cancer.In cellular biology, the role of telomerase is of growing complexity.Telomerase activity is known to be absent in most human somatic cells, as an effect of which telomere shortening is known to occur and thus poses a barrier to tumor growth and marks a molecular path towards aging.But in the majority of malignant cells wherein telomere shortening is staved off as a result of the telomere dysfunction, telomerase activity has found to be high.These contrasting approaches are represented schematically in Figure 6.
Among most human tumors, chromosomal gain and gene amplification involving chromosome arm 3q is found to be more frequent and signifies the involvement of telomerase-associated subunits in malignancies formation [54] [55].Studies suggest that hTERT is frequently expressed during the early tumor  Being the catalytic subunit of the enzyme telomerase, its potential role in promoting tumorigenesis has been reported.In several of human cancer including glioma, melanoma, and bladder, the somatic mutations in the promoter region of TERT have been reported specifically at −124, −146 bp which lie in the upstream region of the transcription start site [90].In many of the recent mutational studies, it has been reported that mutation in the promoter region of the TERT gene is associated with a number of malignancies.1.
It has also been proposed through the studies done so far that carcinogenesis may be promoted by increasing the TERC gene dose, possibly through a different mechanism that is independent of telomerase.TERC gene copy number proves to be a prospective explanation for the increased risk of malignancies, Promoter region of TERT 6.4% [101] which are found in individuals with aneuploidy [102].A study implicates that in amniocytes, retrieved from fetuses with various aneuploidies, TERC copy number has been found to be a parameter of genetic instability [102].A study on telomerase-null mice showed a rapid decrease in the length of telomere during the early cleavage developmental process which proved to be evidence that recombination-based mechanism rather than telomerase is responsible for the lengthening of telomere the early cleavage cycles following fertilization [103].
Telomerase performs a regulatory role in cell immortalization and thus proves to be an attractive anti-cancer target.Therefore, telomere maintenance inhibitors get a considerable interest as a broad-spectrum cancer therapy agent [104].
However, TMM, as well as ALT mechanism, continues to unravel the crossroad pathways towards solving the molecular queries regarding cancer.

Conclusion
Telomere capping plays a crucial role in the maintenance of linear chromosomes, providing protection and stabilization.Telomerase activity has been found to be high in almost all malignant cells which result in telomere dysfunc- Moreover, apart from its role in controlling the cell division through apoptosis, anti-apoptotic functions of TERT need to be methodically investigated.Most importantly, the contribution of ALT cells in tumorigenesis remains to be a fascinating and significant area for further research.

Figure 1 .
Figure 1.Flowchart representing the relationship between telomere loss and apoptosis.

Figure 2 .
Figure 2. Nucleoprotein structure of telomere in mammals.(a) Schematic representation of T-loop structure and associated protein factors.The lariat structure formation is caused due to the looping back of telomeric DNA.As a result of which 3' G strand extension invades the duplex telomeric repeats and the formation of D-loop results as well; (b)The double stranded telomeric DNA is surrounded by Shelterin, a multiprotein complex which includes (TRF1, TRF2, POT1, TIN2, RAP1 and TPP2).TRF1, TRF2 and POT1 are recruited first wherein TRF1 and TRF2 bind to the double stranded region of the telomere and POT1 binds to the single strand overhang.POT1 and TPP combine to form heterodimer complex and bind to the single stranded telomeric DNA overhang.Subsequently, other three additional proteins (RAP1, TIN2 and Tpp1) are recruited to form six subunit complex formation, Shelterin.

Figure 3 .
Figure 3. Formation of G-quadruplex: As a result of replication and addition of telomeric repeat sequences by telomerase, one of the daughter strand (lagging strand) get shortened and thus, results in the formation of 3' single-stranded G-rich overhang.

Figure 4 .
Figure 4. Schematic representation of Telomerase complex formation.(a) Dyskerin, pontin, and reptin combine to form a scaffold for the recruitment of the accessory proteins; (b) The simplified molecular structure of telomerase depicting its associated crucial components.Functional regions of hTERC RNA are also shown.Pontin and reptin dissociate after complex formation.

Figure 5 .
Figure 5. Schematic representation of the telomere maintenance by telomerase.11 complementary nucleotides at the RNA domain of the telomerase act as a template for telomere elongation.It hybridizes with the complementary two telomeric repeats found at the 3' extreme of telomeric DNA.The gap so forms at the 3'end is filled by hTERT component of the telomerase.
bility of DNA polymerase to completely replicate chromosomal ends.Cells are thus restricted to undergo a finite number of cell divisions, marking a checkpoint for creating genome instability and malignant formation[25].As explained earlier telomere prevents the misrecognition of chromosomal ends as DSB (double-strand breaks).Loss of this function is the basis of malignant cells where progressive telomere shortening generates DNA ends which could not be distinguished from strand breaks.As a consequence of which the loss of tumor suppressor alleles and the formation of fusion genes having altered function occur due to chromosomal instability and aneuploidy, ultimately lead to the molecular pathway towards tumorigenesis[3].It has been found that cells become immortalized and start to undergo uncontrolled proliferation by acquiring a telomere maintenance mechanism (TMM), which helps such cells to stave off induction of senescence.Mostly, active role of telomerase in TMM has been observed in almost all human cancer.But, about 5% -10% of immortalized cells escape crisis through a process termed ALT (Alternative Lengthening of the Telomeres)[80] [81].Studies done on telomerase-RNA deficient mice, telomerase-lacking yeast, and in mosquito (Anopheles gambiae) and fruitfly (Drosophila melanogaster) support the occurrence of ALT in such cells[82] [83][84] development and allows the clone to bypass mitotic breakdown and replicative senescence and thus causes the malignant to get immortalized[87] [88].hTERT activity is a key step in hepatocellular carcinoma (HCC) cell proliferation, apoptosis, and immortality[89].Most interestingly, recent studies on hepatocellular carcinoma (HCC) and precancerous tissues, demonstrate a negative correlation between tumor suppressor gene phosphatase and tensin homolog (PTEN) expression levels and telomerase activity[66].PTEN, a tumor suppressor gene with dual-specific phosphatase activity, is a critical signaling molecule in the upregulation of cell proliferation and apoptosis.The study also suggested that PTEN and hTERT have opposing roles in HCC development.It has been observed that expression level of hTERT protein was extremely higher in hepatocellular carcinoma (HCC) as compared with that of the corresponding precancerous tissues which signify hTERT to act as a tumor marker[66].

Figure 6 .
Figure 6.Schematic representation of telomeric effect in two contrasting aspects associated with telomere dysfunction.
tion and thus paves a molecular pathway towards the discovery of telomeres and the associated indispensable telomere maintenance factors.Telomerase related study has still miles to go as molecular dynamicity and assisting protein factors are yet to be explored.T-loop and G-quadruplexes structures which provide stability to the telomeric end may be hotspots for anticancer therapeutics.The investigation of the extra-telomeric functions of the catalytic subunit of telomerase, TERT, has offered new insights into the role of telomerase in cell biology.
of telomeric length is concerned, the studies done in leucocytes found it to be consistent with age, gender and race but the consistency of telomeric [50]th in leucocytes with other phenotypes, such as smoking, socioeconomic status and education, alcohol consumption, body mass index, lipid levels, marker of glucose metabolism, physical activity, diets, and blood pressure has not DOI: 10.4236/aar.2018.75008 100 Advances in Aging Research been found[50].
Mutation in the promoter increases the transcriptional efficiency of the TERT gene.Huang et al.

Table 1 .
Representing mutation associated with promoter region of TERT in various cancers.