Thiol-Disulphide Balance : Could Be a New Marker for Thyroid Cancer ?

Objectives. Cancer is a very widespread disorder known in world wide since long, but its biochemical features remain unclear. Thyroid carcinomas are the most common endocrine cancer and its frequency continues to escalate. There is evidence that the serum concentration of TSH is an unreliant predictor for the diagnosis of thyroid cancer. The formation of the plasma thiol pool from low and large molecular weight proteins suggests that thiol/disulfide balance is important in cancerous cases. The aim of this study was to investigate an oxidative stress marker (thiol/disulphide homeostasis) and IMA (Ischemia modified albumin), Albumin, CEA (Carcinoembryonic antigen), TSH (Thyroid stimulate hormone), thyroxine (T4), free thyroxine (FT4), triiodothyronine (T3) and free triiodothyronine (FT3) in patients with thyroid cancer and compare the results with healthy controls for the first time in literature. Materials-Methods: A total of 43 participants including 23 patients with thyroid cancer and 20 healthy individuals were included in the study. Serum levels of TSH, T4, FT4, T3 and FT3 have been measured during treatment and follow-up of patients with thyroid carcinoma. Serum levels of TSH, T4, FT4, T3 and FT3, IMA, Albumin, CEA, Native thiol (-SH), disulfide (-S-S) and total thiol (TT) as well as disulphide/native thiol and disulphide/total thiol ratios were compared between the groups. Native thiol, disulfide and total thiol concentrations were measured with a novel automated method (Roche, cobas 501, Mannheim, Germany). Results and conclusion: This paper discusses an oxidative stress marker (thiol/disulphide homeostasis) and tumor markers IMA, Albumin, CEA, TSH, T4, FT4, T3 and FT3 in patients with thyroid cancer and compares the results with healthy controls. Mean age at participant was 41.73 years for thyroid cancer patients (21 females/2 males). A control group of 20 participants was included the study (19 females/1 male, mean age 51.75). How to cite this paper: Özdemir, A. and Erel, Ö. (2018) Thiol-Disulphide Balance: Could Be a New Marker for Thyroid Cancer? Journal of Cancer Therapy, 9, 598-604. https://doi.org/10.4236/jct.2018.98051 Received: June 7, 2018 Accepted: August 13, 2018 Published: August 16, 2018 Copyright © 2018 by author s and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/


Introduction
Many pathologies and diseases have been linked to oxidative stress (aging, cancer, cardiovascular disease, respiratory diseases such as asthma and obstructive sleep apnea; diabetes mellitus; rheumatic diseases such as rheumatoid arthritis; urological diseases such as prostate cancer and benign prostatic hyperplasia; neurological diseases such as Parkinson's and Alzheimer's) [1].Thyroid carcinomas are very common and divided into papillary, follicular, medullary, and anaplastic carcinomas.Thyroid carcinomas are malign disease of the thyroid tissue, typically doesn't cause any symptoms early in the disease.But as thyroid cancer grows, may cause symptoms such as a lump in the neck, swelling in the neck, pain in the front of the neck, hoarseness or other voice changes that do not go away, trouble swallowing and breathing, a constant cough that is not due to a cold [2] [3].
Thiols are sulphur analogs of alcohols, taking the suffix "-thiol".They are also called "mercaptans" due to their holding mercury.Sulfides are sulphuric analogs of ethers.Thiol is an organic component consisting sulfid which has a critical role in preventing the formation of any oxidative stress condition in cells [4].
Total thiol in the body is made up of free form of thiol tied to proteins or created by plummeting glutation.Albumin contributes to forming the large part of the thiol tied to proteins.Cells are protected against oxidative damage through thiol and sistein storage found in active parts of proteins such as catalase, superoxyde dismutase,tioredocsin and peroredocsin with thiol glutation at low molecule weight by activating gluation peroxidase and glutation S transferase enzyms.
Cells turn into reversible disulfide bands by oxidizing ROS, cousing oxidative damage, with thiol groups in the medium [4] [5].Thiol and disulfid have roles in stabilization of protein structures (static), regulation of protein and enzym functions, in receptors, carriers, Na-K channel and transcription [4] [6].The dynamic balance between thiol and disulfid play a great role in antioxident defence, detoxification, apoptosize, regulation of enzym activities, transcription and in mechanism of cellular signal transmission.While a large part of plasmatic thiol pool is consisted of albümin and other proteins, a little part of it is made up of low molecule weight thiols such as cystein, cysteinly glycine, glutation, homocystein and γ-glutemilcystein.Abnormal thiol/disulfid balance levels are found in patogenesis of various diseases such as diabetes, mellitus, cardiovascular diseases, cancer, rheumatoid arthritis, chronic renal impairment, Parkinson, Alzheimer, multiple sclerosis and liver diseases [7].Of the low molecule weight thiols, S-glutation (GSH) is searched as a potential biomarker of oxidative stress in some disecises such as renal cancer and diabete, and it is seen at increased level in uremia of the patients with peritoneum dialysis in Type 1 and Type 2 diabet [5] [8].A study argued that thiol-disulfit balance may be a prognostic biomarker in lung cancer; moreover, an ongoing study also claimed that native thiol lowness may have a role in ischemic stroke patogenesis [7].Thiol-Disultif homeostasis has a important role in many disorders.Oxidative stress has been one of the questioned factors in the pathogenesis of cancer in several studies, but its exact [12].The incidence of thyroid cancer is thought to have increased in recent years (from 3.6/100,000 in 1973 to 8.7/100,000 in 2002.In this study show that in patients with thyroid cancer undergoing thyroid surgery were analysed retrospectively in 321 of total 2210 cases [13].When compared to two consecutive time-frame, an increase in incidence of thyroid cancer was observed [14].
Previous studies researched thiol/disulphide homeostasis and oxidative damage in several diseases as a cancer [7]

Study Population
This study was conducted General Surgery Clinic and between January 2018 and April 2018 in Turkey.All of the participants are over the age of 18. Patients that were diagnosed with thyroid cancer.The healthy control group consisted of healthy volunteers, who have without a chronic disease (kidney failure,diabetes mellitus, liver disease, cardiovascular and cerebrovascular disease) and drug use (smoking, alcohol consumption).Venous blood samples (For thiol/disulphide hemostasis tests) were obtained from all participants in the hospital.To separate serum from cells, collected blood samples were centrifuged at 1500 rpm for 10 min.Serum total IMA, Albumin, CEA, TSH, T 4 , FT 4 , T 3 and FT 3 were determined using an automated clinical biochemistry analyzer with original reagents.
Remaining serum samples were stored at −80˚C until all samples were collected.
Native thiol and total thiol were measured by using a new and fully automatic system, disulphide and ratios of disulphide/native thiol, disulphide/total thiol and native thiol/total thiol were calculated (Erel Neselioglu) [6].The study was conducted in accordance with the Declaration of Helsinki 2013 Brasil version and was approved by the Kütahya Ethics Research Committee.All participants provided written informed consent prior to participation in the study.tT 3 , tT 4 , fT 3 , fT 4 , TSH parameters of the participants were their routine parameters at the time they were included in the study and those were recorded from patient files.

Results
Ninety-three patients ( changes in the thiol-disulphide homeostasis may not interact with thyroid hormone values.In this study, the evidence regarding the association between serum thiol/disulphide parameters and T 3 , T 4 , TSH hormon is discussed placing these new findings into context.

Discussion
Oxidative disease.We believe that dynamic thiol-disulphide homeostasis may be a good biochemical marker in following a patient with thyroid cancer.
Our investigation indicated that although thiol/disulphide homeostasis is a relatively cheap, easily available and optionally manual spectrophotometric analysis, this test could not be biochemical marker in thyroid cancer patients.
Our study involves some limitations such as a relatively small number (23 patients and 20 controls) of patients who admitted to a single center.
In this study, we investigated dynamic thiol-disulphide homeostasis status in patients with thyroid cancer for the first time.However, It is required further research on a larger patient population.

Table 1 .
21females and 2 males) with thyroid cancer and 20 healthy controls (19 females and 1 males) were included in the study.The mean age was 41.73 years in the thyroid cancer patient group and 51.75 years in the control group.Clinical characteristics of the patients were summarized in The mean fT 4 level was higher in thyroid cancer patients when compared to the controls (1.22 mg/dL vs 0.98 mg/dL, p = 0.011).There were no differences for the levels of serum IMA, CEA, and TSH between the study and the control 2.00 mg/dL) were higher in patients with thyroid cancer patients (Table2).There were no correlations between serum thiol/disulphide parameters and T 3 , T 4 and TSH hormon in patients with thyroid cancer.It could be said that

Table 1 .
Clinical characteristic of two groups.

Table 2 .
Clinical parameters of the thyroid cancer patients and the control group.
*p value < 0.05 considered significant.Results are given as mean ± SD.