Failure to Recover from Proactive Semantic Interference Differentiates Amnestic Mild Cognitive Impairment and PreMCI from Normal Aging after Adjusting for Initial Learning Ability

Background: There is increasing evidence that the failure to recover from proactive semantic interference (frPSI) may be an early cognitive marker of preclinical Alzheimer’s disease (AD). However, it is unclear whether frPSI effects reflect deficiencies in an individual’s initial learning capacity versus the actual inability to learn new semantically related targets. Objective: The current study was designed to adjust for learning capacity and then to examine the extent to which frPSI, proactive semantic interference (PSI) and retroac-tive semantic interference (RSI) effects could differentiate between older adults who were cognitively normal (CN), and those diagnosed with either Pre-Mild Cognitive Impairment (PreMCI) or amnestic MCI (aMCI). Me-thods: We employed the LASSI-L cognitive stress test to examine frPSI, PSI and RSI effects while simultaneously controlling for the participant’s initial learning capacity among 50 CN, 35 aMCI, and 16 PreMCI participants who received an extensive diagnostic work-up. Results: aMCI and PreMCI participants showed greater frPSI deficits (50% and 43.8% respectively) compared


Introduction
There is a growing concern about the efficacy of traditional paradigms used to diagnose the subtle cognitive changes that emerge during the pre-symptomatic stages of Alzheimer's disease (AD).Once an individual develops cognitive deficits, notably impairment in their memory or thinking abilities, significant brain compromise has already occurred, which hinders treatment effectiveness.To that end, efforts have been made to develop preventive and early targeted interventions for individuals at risk of developing AD.Similarly, the field now confronts the need to develop novel cognitive assessment paradigms that exploit vulnerabilities in persons with incipient AD to aid in early diagnosis and serve as clinically meaningful endpoints from which to establish treatment efficacy.
Ideally, these tests would employ methodologies that are even more cognitively challenging, and are sufficiently sensitive and specific to capture early cognitive changes associated with AD.
A major limitation of most existing memory measurement paradigms such as list-learning tests is that learning is relatively passive and they do not employ controlled learning paradigms during initial acquisition, such as category cues.
Such controlled learning minimizes individual differences in initial learning strategies and the effects of cognitive reserve [1] [2].While there have been attempts to assess proactive and retroactive interference, AD is denoted by specific deficits in semantic memory, and the inability to adequately access semantic lexicon [3].While some traditional list-learning measures attempt to investigate proactive interference effects, most do not emphasize proactive semantic interference (PSI), and more importantly, none investigate recovery from PSI effects.
Recently, we developed a novel semantic interference paradigm the Loewenstein-Acevedo Scales for Semantic Interference and Learning (LASSI-L) [4] [5], which employs controlled learning and semantic cues at both acquisition and retrieval to reduce variability in individual learning strategies, and to maximize the storage and consolidation of 15 to-be-remembered targets in a wordlist (List A).These same cues are employed for learning 15 additional words (List B) belonging to the identical semantic categories as the initial target list (List A).Inefficient learning of the new wordlist may be due to PSI effects belonging to the shared semantic categories.A second cued recall trial of List B targets affords the opportunity to recover from the effects of PSI.The inability to recover from PSI effects after two learning trials is referred to as the failure to recover from PSI (frPSI).FrPSI effects are able to differentiate between aMCI and cognitively Advances in Alzheimer's Disease normal groups [4] [6], which are associated with MRI volumetric reductions in AD prone regions in aMCI adults [7], and regional amyloid load in cognitively normal elders reside in the community [8].
One potential criticism of these previous investigations is that failure to recover from proactive semantic interference, as measured by performance on the second cued recall trial (List B) on the LASSI-L, might be affected by other variables such as poor initial learning capacity or general retrieval deficits.In the present investigation, we employed different statistical approaches to the assessment of PSI, frPSI, and RSI by adjusting for an individual's own initial learning ability, and determined the ability of these corrected PSI, frPSI and RSI values to discriminate between CN, aMCI and PreMCI groups.It was hypothesized that even after adjusting for the strength of initial memory performance, PreMCI and aMCI participants would evidence more difficulties with frPSI as compared to normal controls.No increased difficulties with RSI were hypothesized to occur across groups.

Methods
We recruited 101 English-speaking participants (38 males and 63 females) to the DETECT-pAD longitudinal aging study at the University of Miami Leonard M.
Miller School of Medicine from March 2015 through October 2017.The study was reviewed and approved by the University of Miami Miller, School of Medicine, Institutional Review Board and all participants were formerly consented to participate in the study.All participants were administered a common clinical assessment protocol, the Clinical Dementia Rating Scale (CDR) [9], and the Mini Mental State Examination (MMSE) [10].Memory and other cognitive complaints were assessed by clinical neuropsychology faculty members (RC and DL) and postdoctoral neuropsychology fellows who were blind to the neuropsychological test results and had formal training in administering the CDR.The 101 participants were all community-dwelling older adults who were independent in their activities of daily living, had knowledgeable collateral informants, and did not meet DSM-5 criteria for Major Neurocognitive Disorder, current major depressive episode or any other neuropsychiatric disorder.In cases where there was evidence of cognitive decline by history and/or clinical examination, the clinician scored the Global CDR as 0.5 were considered a diagnosis of MCI based on their examination, pending the results of formal neuropsychological testing.Subsequently, a standard neuropsychological battery was administered and conducted independent of the clinical examination.This protocol included the Hopkins Verbal Learning Test-Revised (HVLT-R) [11], delayed recall from the Logical Memory subtest of the National Alzheimer's Coordinating Center Uniform Dataset Neuropsychological Battery [12], Controlled Oral Word Association Test (FAS) [13], Category Fluency (Animals, Fruits, and Vegetables) [13], the Block Design subtest of the Wechsler Adult Intelligence Scales-Fourth Edition [14], and Parts A and B of the Trail Making Test [15].

Criteria for Cognitively Normal (CN) Participants (n = 50)
Following an extensive clinical interview, participants in this diagnostic group evidenced the following: 1) no subjective complaints related to memory or cognitive impairment during a clinical interview with the participant and collateral informant; 2) no clinical evidence or history of cognitive decline; 3) a Global CDR score of 0 that was determined by a trained clinician; and 4) all memory and non-memory neuropsychological measures scored within normal limits relative to age and education related norms (i.e., less than 1.0 SD below normative values for all tests).

Criteria for PreMCI Participants (n = 16)
Participants in this diagnostic group evidenced the following: 1) subjective memory complaints by the participant and/or or collateral informant; 2) evidence by clinical evaluation or history of memory decline; 3) a Global CDR score of 5; 4) all memory and non-memory neuropsychological measures determined to be within normal limits relative to age and education related norms (i.e., less than 1.0 SD below normative values for all tests).

Criteria for Amnestic MCI (aMCI) Participants (n = 35)
Individuals were classified as aMCI if they evidenced the following: 1) subjective memory complaints reported by the participant and/or collateral informant; 2) evidence by clinical evaluation or history of memory decline; 3) Global CDR score of 0.5; 4) one or more memory measures 1.5 SD or below normal limits relative to age and education related norms.

Loewenstein-Acevedo Scales for Semantic Interference and Learning (LASSI-L)
This novel cognitive stress test uses controlled learning and cued recall to maximize storage of an initial list of to-be-remembered targets that represent three semantic categories.What is unique about the measure is the presentation of another list of to-be-remembered targets that shares the same semantic categories, which are fruits, musical instruments and articles of clothing.Shared semantic categories elicit a considerable amount of proactive semantic interference.Unlike other memory paradigms, the individual is again administered this second list of targets to measure recovery from proactive semantic interference effects.Retroactive semantic interference and delayed recall are also assessed.The specific elements of the test are described below.
The participant is first instructed to remember a list of 15 common words that are fruits, musical instruments and articles of clothing (five words per category).The person is then asked to read the words from the target list aloud, as each word is presented individually at 4-second intervals.In the unlikely event that the person cannot correctly read the word, the word is read by the examiner and the person is asked to repeat the word.If a person does not know one of the words (also unlikely), the examiner tells the person what category the word be-Advances in Alzheimer's Disease longs to (e.g., "Lime is a fruit"), and the person is asked to repeat the word.After the person has read all 15 words, they are asked to recall the words in any order.Following the free recall trial, the participant is presented with each category cue (e.g., clothing) and is asked to recall the words that belonged to that category (LASSI-L A1).The participant is then presented with the target stimuli for a second learning trial with subsequent cued recall to strengthen the acquisition and recall of the List A targets, providing maximum storage of the to-be-remembered information (LASSI-L A2).Following this trial, the participant is exposed to a semantically related list (i.e., List B) that is presented in the same manner as List A. List B consists of 15 words that are different from List A, but belong to each of the three categories also used in List A (i.e., fruits, musical instruments, and articles of clothing).Following the presentation of the List B words, the person is asked to freely recall the List B words; this assesses proactive interference effects (LASSI-L B1).Then, each category cue is given, and the participant is asked to recall each of the List B words that belonged to each of the three categories.Importantly, List B words are presented once again, followed by a second category-cued recall trial.Finally, to assess retroactive interference, the participant is cued to recall the original List A words (LASSI-L A3).This second cued recall trial for the new list allows the assessment of the ability to recover from the initial semantic interference effects (LASSI-L B2).This recovery from proactive interference is a feature of the LASSI-L that is not assessed by any existing list-learning measure [3].Test-retest reliabilities of the LASSI-L have been shown to be high in previous studies, and the accuracy of classification of aMCI patients versus cognitively normal elderly exceeded 90% [4] [5].

Statistical Analyses
Analyses of means for demographic information were conducted using a series of one-way analyses of variance (ANOVAs).Following a statistically significant test of p < 0.05, post-hoc tests of means were examined using the Tukey's Honestly Significant Difference (HSD) test.To control for initial learning effects, a PSI ratio score was developed for both free and cued recall of List B, using the formulas List B1 Free Recall/List A1 Free Recall and List B1 Cued Recall/List A1 Cued Recall.A frPSI ratio was calculated using the formula List B2 Cued Recall/List A2 Cued Recall, and an RSI ratio was conducted using the formula List A3 Cued Recall/List A2 Cued Recall.In addition to PSI, frPSI and RSI ratios, the performance of CN individuals were compared to individuals diagnosed with aMCI and PreMCI using a series of 2 Group (e.g., CN versus aMCI) X Measurement (e.g., total raw score on List A Cued1 Recall versus total raw score on List B2 cued recall).Mixed model repeated measures ANOVA.Main and interaction effects were deemed statistically significant if p < 0.05.The interaction terms in these models were also examined using ANCOVA to adjust for group differences on List A performance so as to evaluate performance on analogous List B measures.
Based on a number of previous studies [3], we defined a LASSI-L decrement

Results
As depicted in Table 1, diagnostic groups did not differ with regard to age and educational attainment.Participants with aMCI exhibited lower MMSE scores as compared to their PreMCI and CN counterparts.There were a greater number of female aMCI participants than CN participants.The aMCI group evidenced lower frPSI ratios than the other diagnostic groups, which indicates that there was a greater failure to recover from PSI even after accounting for initial learning capacity.Despite a statistically significant F-test, group means for the PSI measure did not differ when the Tukey's post-hoc test of means was applied.There were no group differences with regards to RSI ratios.As indicated in Table 2, which compared aMCI with CN participants, there were statistically significant main effects for both Diagnostic Groups and.Measure.This indicates that as a whole, aMCI participants obtained lower scores than CN on measures tapping PSI, frPSI and RSI.Most informative, however, was an inspection of the interaction term.Before statistical adjustment of initial learning capacity, only the frPSI measure obtained statistical significance indicating that greater frPSI effects were obtained for aMCI group.However, after covariate R. E. Curiel et al.Diseaseon List B2 Cued Recall relative to List A2 Cued Recall of 30% or more as indicating difficulties with frPSI.As such, we subsequently examined the extent to which different diagnostic groups scored at an impaired frPSI ratio threshold of 0.70 or less using chi-square analyses.

Table 1 .
Comparison of aMCI and PreMCI subtypes with cognitively normal elders.