Analysis and Prediction of Collateral Channels from Left Superior Vena Cava after Bilateral Bidirectional Cavopulmonary Anastomosis

Objectives: To analyze and predict collateral channels in bilateral bidirectional cavopulmonary anastomosis focused on that from left superior vena cava. Methods: 41 consecutive patients who all underwent bilateral bidirectional cavopulmonary anastomosis were enrolled the study. The number, shape, size, origin and entry, and course of collateral vessels from left superior vena cava were referred for evaluation based on 41 cardiac angiographies. The influential factors on the formation of collateral vessels were identified by binary logistic regression analysis. Results: The number of the left superior vena cava with collateral vessels was larger than right one, 22 versus 8 (X = 10.303, P = 0.001). The medium-distal parts of left systemic vena were prone to development of collateral channel. The logic function with respect to the probability of left systemic venous collateral channel could be expressed as the linear expression of left systemic venous pressure x: 0.418x − 7.111 (X = 23.095, P < 0.001). Setting 17.0 mmHg of left systemic venous pressure as prediction cut-point to discriminate the occurrence of left collateral vessels, the predictive accuracy reached 85.7%. Conclusions: The left systemic venous pressure was an independent factor associated with left systemic venous collateral channel after bilateral bidirectional cavopulmonary shunt. It might be practically feasible to predict the development of collateral vessels according to systemic venous pressure.


Introduction
Bidirectional cavopulmonary anastomosis, which acted as an intermediate stage for the final complete Fontan procedure or as final palliation in high-risk patients with uninventricular physiology, can validly improve the systemic oxygen saturation of patients and reduce the risk of one-stage total cavopulmonary connection.But, bilateral bidirectional cavopulmonary anastomosis should be considered if persistent left superior vena cava was found in patients.That is, persistent left superior vena cava was connected with left pulmonary instead of being ligated up especially when it communicated with superior vena cava insufficiently.
Bidirectional cavopulmonary anastomosis usually leads to the development of collateral channel between the superior vena cava with higher pressure and the inferior vena cava with the lower pressure for decompression of systemic venous pressure [1].In some patients, systemic venous collaterals would cause lethal postoperative desaturation and must be remedied [2].
However, the researches on development of collateral vessels arising from left superior vena cava in bilateral bidirectional cavopulmonary anastomosis were seldom documented in literatures due to the low incidence of the deformity.The study intended to investigate the occurrence regularity and risk factor associated with systemic venous collateral vessels based on the early follow-up results on 41 patients, focusing on collaterals from left superior vena cava in bilateral bidirectional cavopulmonary anastomosis.

Subjects
The present study was approved by Internal Research Ethics Board Committee of our hospital.Patients' parents had signed a series of consent forms in term of the test.Consecutive 41 patients who underwent one-stage bilateral bidirectional cavopulmonary anastomosis were enrolled the study, 20 males and 21 females, median age 7.0 (interquartile range 4.0 to 9.5) years.The criteria were established to select cases with a) functional single ventricle or hypoplastic left heart syndrome, b) the data of postoperative cardiac angiography and catheterization, c) azygos veins ligated during operation and d) additional pulmonary blood flow which was maintained in the repaired main pulmonary with 5 mm of diameter of pulmonary valve orifice.The other clinical characteristics of patients were listed in Table 1.

Data Collection Methods
On Allura CV20 unit (Philips Healthcare Andover, Massachusetts, USA) with St-smart imaging workstation (Crealife, Peking, China), total 41 times of cardiac catheterizations were performed.The static pressure of aorta, pulmonary artery and right ventricle were acquired by right heart technique while the static pressure of bilateral superior vena cava was monitored through jugular vein cannula.The resistance and the volume of pulmonary circulation were calculated from data of pressure monitoring and blood gas analysis based on thermodilution theory.
The morphological observation for superior vena cava, cavopulmonary anastomosis and collateral vessels was undertaken by employing two experienced senior physicians with blinding to test objective.Main assessment aspects involved: the patency of systemic vena and cavopulmonary anastomosis; the number, shape, size, origin and entry, and course of collateral vessels.Each systemic vena was artificially divided into three parts.Two equidistantsegments of systemic vena between anastomotic stoma and originating site of subclavian vein were assumed to be proximal and medium vena cava, while the remainder was regarded as distal part extending cranially.The systemic vena < 80% of the local pulmonary in diameter was defined as vascular stenosis while >120% as vascular World Journal of Cardiovascular Diseases dilatation, with or without distortion.The presence of collateral vessels was confirmed if reported concurrently by two observers.With respect to originating site and drainage destination of collateral vessels, definite agreement was reached by consultation.
Clinical data were collected conventionally.

Statistical Analyses
Retrospective statistical analyses on the data of cardiac catheterization were performed in SPSS 19.0 (SPSS, Chicago, Illinois, USA).Sample size depended on existing cases.The data in normal distribution were reported as mean ± one standard deviation, or else as median with interquartile range.Categorical data were summarized as contingency table, and their relevance to occurrence of systemic venous channel was determined by Pearson's Chi-square test.Being prescreened on the basis of univariate hypothesis test, the influential factors on the formation of collateral vessels were identified by using binary logistic regression analysis.Forward method in the building of a multivariable logistic regression model was adopted by setting entry and removal criterion as 0.05 of probability in software.Receiver operating characteristic curve was employed for evaluating the fitness of binary logistic regression equation.Statistical significance was determined in all process at a two-tailed P value of less than 0.05.

Results
The occurrence of bilateral anatomic abnormality was statistically significant difference (X 2 = 5.959, P = 0.015).24 out of 41 left caval veins presented structure abnormality with not only vascular stenosis (12/41) but also dilatation (12/41).On the contrary, only vascular dilatation (13/41) was found on right caval veins.All the vascular stenosis appeared adjacent to anastomotic stoma, and all the distention of vessel occurred at proximal part of systemic venous above cavopulmonary anastomosis.An example imaging was showed in Figure 1.
The number of the left superior vena cava with collateral vessels was larger than right one, 22 versus 8 (X 2 = 10.303,P = 0.001).27 out of total 35 originating site of systemic venous channel occurred on left superior vena cava.The frequency distribution of systemic venous collaterals is statistically significant different on various parts of systemic vena (X 2 = 33.497,P < 0.001), and them edium-distal part of left systemic vena were prone to development of collateral channel (Table 2, also Figure 1).Preliminary analysis for influential factor associated with the occurrence of systemic venous collaterals by univariate logistic regression methods summarized in Table 3.
The logic function with respect to the probability of left collateral vessels occurrence could be expressed as the linear regression expression of left systemic venous pressure: ln 0.418 7.111 1 with statistical significance on regression model fitness (X 2 = 23.095,P < 0.001), where P in the expression was the probability of left collateral occurrence and x denoted the pressure of left superior vena cava.Left superior vena cava pressure was the major influencing factors on collateral angiogenesis with 1.519 of odds ratio (95% CI: 1.167 to 1.977).Setting the cut-point of 0.5 as prediction probability which was corresponding to 17.0 mmHg of left systemic venous pressure, the predictive accuracy reached 85.7% when discriminated whether the left collateral vessels would occur.The predictive accuracy of the test was investigated by receiver operating characteristic analysis in Figure 2.

Discussion
The incidence of persistent left superior vena cava in congenital heart disease was approximatively 3% -5% [3].Persistent left superior vena cava mainly drains into the right atrium through a dilated coronary sinus [4].However, if it drains directly into pulmonary veins or left atrium, a right-to-left shunt would be occurred [5].It was crucial to recognize this anomaly when Glenn shunt or Fontan procedure is adopted for correcting functional single ventricle.For patients with persistent left superior vena cava, if only right superior vena cava was anastomosed with pulmonary artery, the notable blood from the upper body drained back to the atrium through left superior vena cava, especially when the communication between bilateral superior vena cava was poor.Without blood-oxygen exchange, this part of blood would result in a decrease of effective volume of pulmonary circulation and an increase of ventricular volume load.To   The study showed that the occurrence of anatomic abnormality of left superior vena cava and cavopulmonary anastomosis was higher than that in right structure, and collateral vessels tended to occur on left systemic vein.Several possible reasons accounted for these results.First of all, the most vital thing that should be mentioned was about leaving additional pulmonary blood flow in Glenn shunt.It could inevitably increase the pressure in superior vena cava [6].
Anwar and his colleagues had reported that additional pulmonary blood flow through the main pulmonary trunk was inclined to flow into the left lung, which aggravated the burden on left lung [7].Besides, pulmonary vascular bed was smaller in the left lung of two lobes than in the right lung of three lobes.The left lung presented with more weaken compensatory ability to adapt to sudden change of the vessel reconstruction by surgery with relatively higher pulmonary resistance.Furthermore, when persistent left superior vena cava developed insufficiently compared with superior vena cava, it was mismatched with the left pulmonary artery both in diameter and impedance [8].As reasons mentioned above, it could be deduced that the flow in left superior vena cava would be slower.Based on Bernoulli equation, the slower flow can contribute to increasing hydrostatic pressure which might be an independent risk factor related to the presence of collateral vessels.
Our results showed that the occurrence of systemic venous collaterals following bilateral cavopulmonary anastomosis (65.9%) was significantly higher than that in the priorstudy either on cavopulmonary anastomosis (33%) or on Fontan procedure (20.2%) [9] [10].It implied the difference in circulatory physiology between cavopulmonary anastomosis with and without left super vena cava.Maybe, some other fluid dynamics mechanism not involved in the study could better explain it.Some impact on collaterals development, such as unbalanced pulmonary blood distribution, "liver factor" and weaken-pulsatile flow in superior vena cava, should be further investigated intensively by large samples study in future.
For patients who suffered from functional single ventricle, Salim and his associates reported that the proportion of the superior vena cava blood accounted for total cardiac output was about 55% -60% when 2 -3 years of age, then gradually decreased to 35% up to about 6 years old [11].Thus, on Glenn shunt, reduced blood in superior vena cava directly leaded to a reduction of effective pulmonary volume with age until pulmonary circulation resistance tended to be stable.pressure was easy obtained in care unit, it must be practically feasible to predict the development of collateral vessels according systemic venous pressure.However, we did not obtain similar relationship between right systemic venous pressure and right collaterals occurrence.It might be supposed that systemic venous pressure was not higher enough to be a risk factor of collaterals formation.
Several limitations would weaken the accuracy of the results as we anticipated.
Firstly, potential confounding factors were not mentioned in the study, such as severity of illness, physical state of patients, etc.Moreover, small samples in the study did not completely cover all the type of collaterals drainage channel.It would seriously restrict the variables selection in binary logistic regression analysis.Another limitation was high operation age of patient cohort in study.
However, it was commonly opinion that bidirectional cavopulmonary anastomosis should be performed early in life in order to provide early volume unloading [13].Last but not least, the work did not investigate the long-term effect of systemic venous collaterals flow on patients because of retrospective design.
The associations between potential risk factors and the development of collaterals were required to be illustrated in future.

Conclusion
The work supplements the knowledge of systemic venous collaterals arising from left superior vena cava in bilateral bidirectional cavopulmonary anastomosis.It would benefit surgeons to accurately understand and identify the presence of venous collateral channels.

Figure 1 .
Figure 1.Angiogram example of systemic venous collateral channel.(a) (b) Angiogram of left caval vein on a patient who suffered from functional single ventricle and underwent bilateral cavopulmonary anostosis at 7 years old.Two notable collateral vessels were found, which originated from dilated caval vein accompanied with anastomotic stricture.One drained blood into inferior vena cava via pericardiophrenic vein in anterior mediastinum, the other via internal thoracic vein in mediastinum medium (upper panel); (c) (d) Another case with systemic venous collateral channel after surgery at five years old.azygous venous system and paravertebral venous plexus were main drainage pathway (lower panel).

Figure 2 .
Figure 2. Logistic regression analysis.(a) Relationship between the pressure of left superior vena cava and the occurrence probability of systemic venous collateral.It results from binary logistic regression; (b) Receiver operating characteristic curve for evaluating the fitness of binary logistic regression equation.Area under the curve is 0.891, and P value is below 0.001 as null hypothesis is that true area = 0.5; (c) Available prediction cutoff of left superior vena cava pressure in receiver operating characteristic analysis.Optimal sensitivity and specificity are obtained while cutoff value is 17 mmHg.The point was closer to the top-left part of the receiver operating characteristic curve.

Table 2 .
Frequency distribution of systemic venous collaterals.
Pearson's Chi-square test is used to determine if the frequency of systemic venous collaterals is different between various originating position on caval vein, various draining pathways and various diameters.The numbers in the parentheses in 3th column are frequency of left systemic venous collaterals.P values < 0.05 were considered statistically significant.RSVC right superior vena cava, LSVC left superior vena cava.

Table 3 .
Preliminary analysis for influential factor associated with the occurrence of systemic venous collaterals by univariatelogistic regression methods.
The influential factors on the formation of collateral vessels were prescreened by using univariate logistic regression methods.The factors of P values < 0.4 in score test for regression model (marked as bold) were considered enter multivariable logistic regression analysis.
[12]ulmonary small arteries might irreversibly lead to an increase of pulmonary resistance and static pressure that promoted the formation of venous collaterals.In summary, the vena cava collaterals might increase over time especially on patients who underwent operation after 6 years old.Unfortunately, because of very short follow-up period, we had no evidence to convince the fact that the interval since surgery to study was the influential factors associated with the development of collateral channels.It was difficult for Doppler ultrasound to get a clear image of collateral vessels because echo intensity was prone to disturbance by air in lung fields and because available sound/blood angle was hard to obtain[12].We proposed tentatively simple way to predict the occurrence of collateral vessels derived from left superior vena cava in bilateral bidirectional cavopulmonary anastomosis based on left superior vena cava pressure.The predictive accuracy reached 85.7% when set 17.0 mmHg of left superior vena cava pressure to determine whether the systemic venous collaterals were present.If the predictive accuracy was verified by a mount of practical results later, this method would provide an objective basis for surgeons to prescribe further treatment on patients.Because central venous anastomosis.It implied a steady tendency of development of venous collaterals.But on the other hand, pulmonary vessels growth appears retarded, and hypoxia condition seems to be deteriorative with patients grew up.Consequently, patho-B.Si et al.DOI: 10.4236/wjcd.2018.84023245 World Journal of Cardiovascular Diseases logical changes