Flower Characteristics and Pollination Behavior of Euryale ferox ( Salisb . )

The Euryale ferox (Salisb.) or gorgon or makhana is one of the most important non cereal food crops of commerce from wetland ecosystem in India. Flower is cleistogamous (CLS) and predominantly self-pollinated. The variations in floral characters were observed in 10 types of germplasm viz., Manipur-2, Manipur-4, Manipur-7, Manipur-9, Selection-17, Selection-23, Selection-27, Selection-28, Superior Selection-1 and cv. Swarna Vaidehi. In our present study, the number of flowers varied from 8.33 (Manipur-9) to 16.33 (Superior Selection-1) per plant and flowering period was about 40 days. However, peak pollination was observed between 60 70 days after transplanting. The weather of August and September were ideal for pollination and fruit set. The temperature and humidity of this period were 29 ̊C 31 ̊C and 79% 81%, respectively. Besides cleistogamy (CL), chasmogamy (CH) is also observed after July flowering in Euryale, when crop gets matured, water level considerably goes down, and flowers are generally opened in air. There were rare chances for cross pollination by insect. In later stage, chasmogamous (CHS) flower increases up to 22.50% in October. Seed formation of the CHS flower was very less and seed number varies from July (11.25/fruit) to September (28.33/fruit). Artificial hybridization can be performed in CHS flower. The complete flower development was noticed within 72 96 hrs from floral initiation. Therefore, getting of CHS flower outside water is very less. There were strong correlations between number of embryos (r = 0.762), ovary area (longitudinal) (r = 0.681) with the yield of the Euryale plant.

China.However, wild forms of plants in wetland ecosystem were also found in Korea and Japan, as well as parts of eastern Russia [1].During spring, it grows in water and produces bright purple flowers during May.It is a photo insensitive crop and can be grown successfully from March to September in economic way in India.Floating leaves are prickly on petioles and along veins and the underside of the leaf is purplish, while the upper surface is green.All the plant parts are thorny outside.It thrives best in ponds, wetlands, lakes and lowland in tropical and subtropical regions of India.Field cultivation of Euryale is also recommended where plenty of water is available.Euryale is famous for its [2] high valued seeds marketed in India, China, and Japan [3].In India particularly in Bihar, more than 96,000 hectares were under cultivation in 1990-1991 [2].But in present condition it is cultivated about 11,000 ha only [4].According to [5] it is now becoming under extinction due to rapid urbanization and profitable fish production in wetland area instead of Euryale, which is labor intensive.In Mithila culture of Darbhanga, Euryale or makhana is known for its auspicious ingredient used in offerings to the Goddesses during "Durga Puja" festivals.Evidence from archaeobotany indicated that Euryale was a frequently collected as wild food source during the Neolithic period in the Yangtze region [6].The earliest record showed that use of Euryale ferox to date was found in Gesher Benot Ya'aqov, Israel, among artifacts of the Acheulean culture 790 -750,000 years ago [7].Edible seeds of Euryale are used in traditional Chinese medicine, where they are often cooked in soups along with other ingredients [8].Though it is an eastern and south Asian species, it is widespread and recorded from the Russian Federation, India (Assam, Bihar, Jammu and Kashmir, Manipur, Meghalaya, West Bengal), Japan (Honshu, Kyushu), Republic of Korea, Taiwan (Province of Thailand), Bangladesh and Myanmar [9] [10].This plant has edible and medicinal uses.Makhana kernel powder is said to have control of cardiovascular diseases [11].It is taken internally in the treatment of chronic diarrhoea, vaginal discharge and kidney weakness [12] [13].The genera Euryale of east Asia, and Victoria from far South America, are closely related despite their geographic distance [14] [15] [16].Flower is epigynous with more than 40 corolla [17], ovary 7 -16 chambered with 6 -8 seeds/locule [5].
The objectives of this study were to understand about the flower structure,

Materials and Methods
The experiment was carried out at ICAR-RCER, Research Centre for Makhana, Darbhanga, Bihar, India, during 2017.Makhana was cultivated from April onwards to October.Flowering period covered from Mid May to November.In our American Journal of Plant Sciences To obtained hybrid seeds from hybridization, a few precautions were taken as it was wetland aquatic crop and after artificial hybridization, hybrid fruit generally submerged again to mature the seeds after 7 -10 days of pollination.Emasculation and hybridization were performed safely.Water level was maintained 10 -15 cm during hybridization period so that pollinated flowers did not damage.

Results and Discussion
A close perusal of the Table 1 revealed that the number of flower/plant varies from 8.33 (Manipur-9) to 16.33 (Superior Selection-1).These results were in conformity of the study conducted by [19].The maximum floral parts were also observed in Superior Selection-1 (calyx + corolla + androecia = 4 + 42 + 127).
The minimum floral parts were found in Selection 17 (calyx + corolla + androecia = 4 + 22 + 68).There were positive correlations with different floral parts to yield.On the contrary, Manipur-2 also produced little flower but its yield capacity was higher due to production of increased number of flower/plant.Flower size ranged from 23.16 cm 2 in Selection 17 to 33.79 cm 2 in Superior Selection-1.
Flower stalk length varies significantly among the germplasm whereas as flower stalk girth did not.Zhuang [5] also reported that a flower of Euryale was about 5.0 cm diameter and outer skins of the flowers are densely pricked.Data pertaining to Table 2 enable us to explain that the highest innerovary area was noticed in Superior Selection-1 (6.62 cm 2 ) followed by Swarna Vaidehi (6.24 cm 2 ).
The germplasm Selection-17 had less number of colored corollas (Violet-10) whereas Superior Selection-1 had the maximum (Pink-22).According to [17], the total corolla was greater than 40, which was further confirmed by [7].In the present study, we found that minimum locule 11 and embryos 4.8 which were found in Selection 17 whereas Superior Selection-1 had the maximum locule of 17 with 8 embryos/locule.[17] stated that multicarpellary, syncarpous inferior ovary which had 7 -12 locules were present in Euryale.According to [5], in Euryale ovary was 7 -16-loculed, each locule with 6 -8 or more ovules.Flowers of Euryale were epigynous, petals outer purple, violet or pink fading to inner white and gradually smaller (oblong-lanceolate) and tapering large calyx kept the flower air tight in water.This result about flower structure was confirmed by

Euryale germplasm.
All floral characters were correlated with yield, which is calculated from number of fruit, number of seed and average weight of seed.It has been found that besides flower stalk, girth of stalk and number of calyx, all the quantitative characters were highly correlated with yield.There was a strong correlation between number of embryos (r = 0.762) and ovary area (longitudinal) (r = 0.681) with yield (Figure 5).According to [25] among 14 traits studied, 100-seed weight was observed to have highest contribution (57.30%) towards genetic divergence followed by seeds/fruit (18.25%) and fruit diameter (17.46%).Stigma was sessile and stigmatic color varied from light yellow (004D) to orange red (032A).The flowers having deeply colored stigma produced less yield as compared to light colored once.

Conclusion
From the present study, we concluded that CLS flowers are appropriate for commercial cultivation.Hybridization can be possible through CHS flower as these flowers are produced at later stage of plant growth and development and opened in air before dehiscence of anther.These flowers are small in size and produce less seeds in their fruits.For hybridization program water label of the field must be maintained 10 -15 cm.After hybridization flowers were tagged and kept in air for 7 -10 days for healing after emasculation injury.After that again water level was increased to submerge the fruits to get mature hybrid seeds.Flowers having a greater number of floral parts i.e. calyx, corolla and androecia produced the higher yield.The flower size and inner ovary area had the positive correlation to yield.

B
. R. Jana DOI: 10.4236/ajps.2018.94057723 American Journal of Plant Sciences uatic medicinal plants native to south east or eastern Asia, particularly India and germplasm block, 28 germplasm of different morphological structures collected from different parts of India were the basic materials for study.We have selected 10 outstanding selections with differences in floral structures and pollination behaviors were studied.The floral characters like number of calyx, number of corolla, androecia, sessile ovary with different stigmatic color and ovaries with different chambers and their ovules were studied thoroughly.Complete Randomized Design (CRD) was followed to analyze the data statistically for flower characters.Fisher protected LSD (P > 0.05) was adapted to analyze the data on pollination % and duration of pollination and seed formation (number) in different types of pollination.Plant yield performance and yield correlation with flower morphological characters were delineated statistically by using Karl Pearson Correlation coefficient.Stigmatic color was recorded by UPOV color chart[18].Favorite pollination periods with temperature range were recorded for better flowering and fruiting.To study the chasmogamy, air opened flowers before dehiscence of anthers were tagged from July to Nov and number of flowers to total numbers of flowers also calculated including their seed.Pollination % and different flower percent was calculated by the following sequence:

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[21] [22].After transplanting flowering needs and 45 days and flowering periods last for 40 days and peak flowering was observed between 60 -70 days (Figure1and Plates 1-4).Arrangement of calyx and corolla was so nicely designed it paves the way to CL, assured self pollination before opening of the flower and in Euryale self pollination happened when flowers remain closed and with in water.By destructive method, we found that flower bud emerging out of the bract and after 3 -4 days, self pollinated, i.e.CL occurred.CH i.e. self pollination in open flowers happened after July onwards when the water level of the field drastically reduced and in terminal 4 th -5 th flowers CH occurred.The terminal 1-3 rd flowers were
) American Journal of Plant Sciences

Figure 4 .
Figure 4. Euryale flowers and pollination in relation to a particular weather [CRD with 3 replications for characters pollination % and duration of pollination].
time and per cent pollination of Euryale in relation to weather.Dif-

Table 1 .
Physical characteristics of flowers of Euryale in wetland ecosystem in north Bihar-2017.

Table 2 .
Dissected flowers of Euryale and their characters under wetland ecosystem of north Bihar-2017.