Climate Change Favors Grapevine Production in Temperate Zones

Wine production has a long-standing history in Palatinate (Southwestern Germany), dating back to Roman times. Especially “Riesling”, but also several “Pinot” varieties gained major significance. Red wine varieties gained prominence over the last 20 years only, which may be a consequence of climate change. Our objective was to review temperature and rainfall data over the last 40 years, measured “on-winery” in Hainfeld and correlate these data with grapevine growth parameters: the development of bud break, flowering, veraison and harvest dates, yields and grape sugar concentrations of “Pinot Gris”, “Pinot Noir”, “Riesling”, “Silvaner” and “Müller-Thurgau”. Since the 1970s, bud break, flowering and veraison are 11 15, 18 22 and 16 22 days earlier; harvest dates are 25 40 days earlier. Sugar concentrations increased significantly, but yields decreased. Annual rainfall has not significantly risen, while the mean annual minimum and maximum air-temperatures rose by ~0.9 ̊C, and by ~3.4 ̊C, respectively, resulting in an average increase of the mean annual temperature of ~2.1 ̊C. Remarkably, both mean monthly minimum and maximum temperatures rose especially in springtime, which should have been the driver for earlier bud break and flowering. A change in the climatic conditions, therefore, appears to be one of the key reasons for more favorable grapevine production in Palatinate, especially for “Pinot Noir”, which showed the highest increase in sugar concentrations. The Huglin-index, a measure for the suitability of growing specific grapevine varieties in given environments, increased from 1685 to 2063. According to this index, the climate change may be favorable already for growing grape varieties so far rarely grown in temperate zones, such as “Cabernet Sauvignon”, “Syrah” and “Tempranillo” that are more suited to warmer, Mediterranean climates.


Introduction
Climate change will undoubtedly affect global crop production in terms of productivity, as well as the relative distribution, and suitability, of particular crops and varieties of these crops, including grapevine and wine production.The changes in temperatures and rainfall are key drivers in this respect, which directly impact on crop development and productivity, but also in respect to changes in pest and pathogen spectra [1] [2] [3] [4] [5].Wine production in the regions of southwestern Germany, northeastern France and northern Switzerland, along the Rhine River and its tributaries, such as the Aare, Thur-Ill, Neckar, Nahe, Main, Saar-Mosel and Ahr, has been a long-standing tradition, dating back to the Roman times.Traditional white grapevine varieties, such as "Riesling" and "Silvaner" or several "Pinot" varieties, are characteristic of these wine growing areas, and the wines produced here have achieved worldwide recognition for their quality and distinction [6].Some more recent introductions, as "Müller-Thurgau", also referred to as "Rivaner" or "Riesling × Silvaner", which was crossed in Geisenheim (Germany) between "Riesling" & "Chasselas" and propagated first in Wädenswil (Switzerland) [7], or "Dornfelder", developed in Weinsberg [8] [9], have also achieved major recognition from the region.In Southern Palatinate, represented by the German Wine Road, the traditional and more recently introduced varieties have provided significant success in the global arena for winegrowers [6].
General observations indicate that in Palatinate, over the past few decades, harvest dates appear to have gradually shifted, becoming earlier, while the proportionate area of production for red wine has increased, including an expanded range of red wine varieties.This includes the new variety "Dornfelder", but also traditional varieties more associated with warmer wine growing areas, such as "Merlot" and, more rarely, also "Cabernet Sauvignon" from Bordeaux (Southwest of France) or "Syrah" from Côte du Rhone, Languedoc, Toscany and Sicily (Italy).These observations indicate that, among other factors, such as new cultivation and management techniques, and/or improved wine-growing skills, changes in climatic conditions may be contributing to the shift from more traditional to more "exotic" grape varieties [10].
In the current study, we assessed 40-years' worth of comprehensive field data and measurements on climate, phenology and crop growth parameters recorded by the principal author in Hainfeld, Southern Palatinate, Germany.The main objective was to correlate the climatic data with the phenological and harvest data for five major grapevine varieties of the region, assess the implication of the results to current regional crop growing practices and the influence of climate change to future challenges on a local, regional and global scale.

Vineyard Site Details and Grapevine Varieties
The winery Kastanienberg in Hainfeld (Südliche Weinstrasse, Palatinate, Ger-many; 49˚15'N; 8˚06'O) is located directly on the "German Wine Road", within the wine growing region Palatinate.This route, as with the "Route du Vins d'Alsace", which is approximately 100 km further South, extends for over 80 km along the Rhine valley on gentle hills, between Triassic "Bundsandstein" mountains and the river, comprising approximately 229 km 2 of winelands.
For this study, four varieties of white wine ("Riesling", "Silvaner", "Müller-Thurgau", "Pinot Gris") and the red wine variety "Pinot Noir" were selected from the winery to investigate phenological and first annual harvest dates, grapevine juice concentrations (measured on-winery as juice density in ˚Oechsle) and the annual juice quantities in hL per hectare since 1975.The varieties differ, among other characters like sugar and acid formation or suitability in specific soil types, also in dates of ripening: "Müller-Thurgau" has been counted as a early ripening variety in Germany, while traditionally "Silvaner" were attributed as "middle late", and "Riesling", "Pinot Gris" and "Pinot Noir" as "late ripening".In this way, we covered the whole range of traditionally grown, important grapevine varieties with respect to early or late ripening and harvest dates.
For each grapevine variety a single vineyard was selected, planted in the 1970's, or vineyards where old rootstocks had been replaced by rootstocks of the same variety and, if possible the same clone of the same variety.The "Pinot Gris" was planted in 1975 and replanted in 2014.The "Riesling" was planted in 1968 (old "Bürklin" clone) and replanted with in 2005.The "Silvaner" (clone 26/93) was planted in 1968, and replanted in 2006.The data of "Müller-Thurgau" are from four vineyards in total, planted in the 1970-1980's, but in different years.
The "Pinot Noir" vineyard was planted in 1989, which provided a first harvest in 1991.
In Hainfeld, the soils are predominantly silty to loamy Luvisols and sandy to clayey Cambisols, generally with a moderate to high soil fertility and nutrient availability.Geologically located in the depression zone of the Rhine valley between Basel, Strasbourg and Mainz, the soils have developed on a wide range of bedrocks from the Triassic period and are partly covered by sandy-alluvial or silty-Loess material from the Quaternary.Thus, they cover a diverse range of sandy to clay soils, of quite different pH and water holding capacity, representing very well a manifold Terroir [11] of Palatinate on a very local scale.

Phenological and Harvest Data
The dates for bud break, flowering (onset) and veraison (= onset of ripening) were recorded annually since 1974 for the five cultivated varieties grown in Hainfeld.Since 1974, also the harvest dates, harvest quantities (hL grapevine juice per ha) and grapevine sugar concentrations (measured as juice densities in ˚Oechsle) were documented annually since 1975, starting from the first harvest for each grown variety.

Climatic Data Obtained at Winery
Daily rainfall was measured by a standard rain gauge all year long since 1970.
Since 1975, daily minimum and maximum temperatures were measured daily, throughout the year.Mean annual temperature was calculated by the average of the mean annual maximum and mean annual minimum temperature.Daily minimum and maximum temperatures between April and September are the base to calculate the "Huglin index", a specific climatic index for wine growing areas.It was calculated according to Huglin [12] using the following equation: where K is in this formula a latitude dependent coefficient (here K = 1.05825 for latitude 49˚15'N), T min is the daily minimum temperature, T max the daily maximum temperature, and T average the daily average temperature, calculated for daily between 1 st April and 30 th September of each year.For this study, we calculated the Huglin index annually between 1975 and 2015 for the village Hainfeld, based on our data from the winery.

Statistical Analyses
Linear regressions were applied to data to assess for date changes in bud break, flowering and harvest, annual sugar concentrations and harvest quantities per grapevine variety.We calculated the mean monthly and annual precipitation , and the mean monthly and annual minimum and maximum temperatures, and, as a consequence of the two later data sets, also the daily, monthly and annual average temperatures and the Huglin index (see above) for the village Hainfeld (Pfalz).Beginning 1975, we summarized data into 10-year blocs (1975-1984, 1985-1994, 1995-2004, and 2005-2014) and conducted ANOVA followed by Fisher's Least Significant differences (LSD), and-without the blocs-linear regressions, to determine monthly and annual rainfall and temperature changes over time.

Sugar Concentrations and Harvest Quantities in the Grapevine Varieties Since 1975
Juice densities were slightly elevated in the varieties "Riesling", "Silvaner" and "Müller-Thurgau" since the 1970's, but with relatively low r 2 -values of 0.14 -0.18 (p = 0.001 -0.01; Figure 1 for these varieties (Table 1).Sugar concentrations did not increase in "Pinot Gris" (r 2 = 0.01; p = 0.63), which had the highest sugar concentrations of the white wine varieties since 1975 without exception in any year.Remarkably, for  obtained for all five varieties (r 2 = 0.38; p = 0.001; Figure 1(a)).

Grapevine Harvest Dates Since 1975
The grapevine harvest dates clearly changed for all five varieties investigated (Figure 1(c)).The r 2 -values were high for all white wine varieties (0.50 -0.72), and lower for "Pinot Noir" (0.36), which had not been grown on the winery prior to 1988.Initial harvesting dates switched from early/mid October to mid September for "Müller-Thurgau", and from end October/early November to early October/end September for "Riesling", "Silvaner" and "Pinot Gris" (Figure 1(c)).This corresponds to an approximate 26 days earlier harvesting date for "Müller-Thurgau", 27 days for "Riesling", 29 days for "Silvaner", and 37 days for "Pinot Gris" between the decades 1975-1984 to 2005-2015 (Table 2).For "Pinot Noir", no data existed for 1975-1990, but a similar trend was recognized as for "Pinot Gris" between 1991 and 2015 with harvests conducted approximately 10 days earlier over the period of study (Figure 1(c)).When data on the date of harvest

Bud Break, Flowering and Ripening Since 1975
Bud break, flowering and ripening ("veraison") dates clearly changed for all five varieties over the period of study (Figure 2).Following linear regression, bud break across varieties became 11 -15 days earlier since 1975, flowering 18 -22 days and onset of ripening 16 -22 days.The r 2 -values were 0.27 -0.43 (p < 8.6*10 −4 ), except for "Pinot Noir" for bud break, which was planted later (r 2 = 0.17; p = 0.028).The bud break dates generally switched from end April/early May to end/middle April, flowering dates from late June/early July to early/middle June, and veraison from early/middle September to middle/end August (Figure 2).

Annual and Monthly Rainfall
Linear regression did not reveal any significant change in annual precipitation between 1970 and 2015 (Figure 3), although there was relatively lower precipitation in the first decade (573 mm).The mean annual precipitations recorded were 643, 700, 722 and 686 mm within periods of 1975-1984, 1985-1994, 1995-2004 and 2005-2014, respectively (Table 3).Linear regressions did not reveal any change in monthly rainfall over the 45 years for any of the twelve months (data not shown).However, remarkably, ANOVA showed clear differences in rainfall between the months and a bimodal precipitation curve throughout the year, with   increased rainfall during late spring to early summer (May to July) and especially at the end of autumn to early winter (October to January, Figure 4).Since 1970, rainfall was lowest in April, August and September (41, 47 and 50 mm), and highest in November and December (65 and 74 mm, respectively).

Mean Annual and Monthly Temperatures and Huglin Index
Mean annual temperatures generally increased between 1975-2015 (Figure 5).
Mean maximum temperatures increased from 14.1˚C in 1975-1984 and 1985-1994 to 16.8˚C in 2005-2014, and the mean annual minimum temperatures increased from 6.9˚C to 7.6˚C in the same period (Table 3).This resulted in an increase in the average annual temperatures, from 10.5˚C to 12.2˚C between 1975-1994 and 2005-2014 (Table 3).Linear regressions of the mean annual   Analysis of the data in our study shows that the mean annual temperatures in Hainfeld have increased by 1.7˚C -2.5˚C over the last 40 years, a figure substantially higher than the global land-surface air temperature rise of approximately 0.18˚C per decade since 1950 [19].Both mean monthly minimum and maximum temperatures show that the temperature increases especially during spring (April and May), which relate to bud break and the beginning of flowering.Furthermore, the mean annual maximum temperatures have increased throughout the year, which would tend to relate to a faster plant development and to higher rates of photosynthesis, and thus sugar production of the grapes during the vegetation period, as evidenced in the study.Also, the Huglin index demonstrates an increased suitability for production of grapevine varieties that are more suited to warmer Mediterranean climates, especially for red wine production.The increasing mean annual temperatures since 1975 and the overall climate change in Hainfeld strongly correlate with the significantly earlier bud break, onset of flowering and veraison.Our findings similarly correspond with trends for "Riesling" shown by Duchêne & Schneider [13] obtained for the period of 1972-2002 in Colmar (Southern Alsace, France).
Four of the five grapevine varieties appear to have responded to this increase in temperature by producing higher juice sugar concentration, and by consequence, a higher wine quality, while for the fifth variety, "Pinot Gris", harvest has become five to six weeks earlier than 40 years ago.However, in some of the warmer and dryer years a decrease of natural grapevine acids, especially in white wines, has led to complaints [15] [20], resulting in governmental permission to artificially correct for such acid deficiencies in Germany for specific years.In response to this change, efforts have been initiated to identify in particular new "Riesling" and other clones of traditional white wines that will be more suited to the warmer climate, than the traditionally used clones [21] [22].In the past one of the challenges in the North of the Alps was to find clones of traditional white B. Koch, F. Oehl DOI: 10.4236/as.2018.93019259 Agricultural Sciences wine varieties or new varieties that would ripen earlier [7].Nowadays, it has become more favorable to look for or recover those clones/varieties that ripen later [23].According to Schultz & Jones [18], the projected temperature rise will alter wine composition and wine styles, and combined with the predicted changes in rainfall volume and timing, will further challenge viticulture in the future.Similarly, the same and other authors [18] [24] expect that such changes in the climate will result in a shift in grape growing boundaries.For our situation in Hainfeld, we can summarize that over the last four decades temperature has already changed significantly, while rainfall has remained relatively consistent.Further temperature increases of 1˚C -2˚C within the next 20 -50 years, as can be expected, will certainly affect the traditional white wine production of southwestern Germany, and lead to a further increase in the red wine production, through more favorable conditions.The shift from white to red wine production in Palatinate will likely be based on temperature increases in the region.The Huglin indices measured during the last decade also indicates that red wine varieties previously considered "exotic", such as "Syrah" and "Tempranillo", can now be grown successfully in Palatinate and neighbored regions [25] [26].It is likely that with further changes in climate, varieties such as "Merlot" and "Cabernet Sauvignon", will gain further prominence in the region and compete successfully with traditional varieties, such as "Portugieser", "Pinot Noir" and "Dornfelder" that are more adapted to the so far "cold grapevine climates" of approximately 10˚C mean annual temperatures.Such low mean annual temperatures have become increasingly rare in grapevine growing southwestern Germany within the last twenty years.
An increasingly warmer climate will certainly not only affect the range of grapevine varieties and the wine quality in colder climates, such as in Germany and neighboring countries, but also the occurrence of grapevine pests and pathogens originating from warmer climates [4].Some are already being detected increasingly in Palatinate or can be expected soon, such as the American grapevine leafhopper, or flavescence dorée disease, which has already been detected in western Switzerland [27] [28].Other, more traditional pests and pathogens may reduce in importance, as temperatures increase e.g.downy mildew [29] [30].However, there is no escaping the fact that since the introduction of viticulture into the Rhine Valley Region by the Romans, wine production has been a continuous challenge, with repeated climate changes since that time [2].Nevertheless, it seems that current changes are at least partly "man-made" and that rates of change and consequent challenges may have accelerated and/or multiplied.
Simulation of the impact of climate change on the distribution of the most important European wine regions has demonstrated the potential for a dramatic change in the landscape for wine production in Europe and worldwide due to changes in climate, especially in warmer grapevine growing areas [3] [31].Other studies, however, argued that changes in climate will not dramatically affect the suitability of viticulture in the main wine-growing regions before 2050 [32] [33], or for other important fruits, such as olives [34].Impacts on photosynthesis, fruit and wine quality, do not necessarily depend only on climatic conditions [35], but may regionally and locally be affected for instance by soil characteristics [36], or water availability.One major constraint for the future of grapevine and wine production certainly concerns water status [37].With increasing temperatures, the need to irrigate vineyards may become more crucial, especially where water is a limiting factor.There are certainly several extreme examples within the world, where wine production systems are almost 100% dependent on irrigation or on efficient nocturnal condensation, such as in the semi-arid, sub-tropics and tropics.Moreover, extreme conditions in hot wine-making regions is likely to have an overall negative impact on wine quality [24] [38], e.g. by altering the composition of organic compounds in the juices and wines, leading to unfavourable ratios of sugar and acid concentrations, and the amount and composition of phenolic compounds [39].
Comparing our data with those of Duchêne & Schneider [12], we can conclude that in the Rhine valley between Basel, Freiburg, Colmar, Strasbourg, Landau, Neustadt and Geisenheim, at least local and regional warming has already reached or even passed 2.0˚C within the last few decades.It can be further concluded that global warming could accelerate over the next 20 -50 years.We therefore assume that grapevine and wine production can dramatically change further within this century, especially in warm and arid wine growing regions [3].However, not only grapevine, or olive production face such problems, but across the agricultural spectrum climate change will variously affect production and quality, especially in semi-arid to arid, warmer climates, where thousands of hectares of productive agricultural land have already been lost due to severe drought, desertification, salinization, heavy rain events, flooding or erosion.

Conclusion
Over the previous four decades, mean annual and mean monthly rainfall have not significantly changed in Palatinate; mean annual and mean monthly temperatures have significantly increased however, especially during the vegetation period of grapevine, significantly affecting grapevine growth, phenology and juice quality.Climate has changed and most likely will further change wine production in Palatinate, and in other traditionally cooler European grapevine growing areas, such as Alsace or Franconia, towards more favorable conditions for successful red wine production, but at the expense of the traditional highly appreciated white wines, especially "Riesling" and "Silvaner".The changes in climate will furthermore affect the pest and pathogen spectrum.In conclusion, researchers need to be cautious in many aspects to protect and develop further the grapevine production in these traditional wine growing areas north of the Alps.

Figure 2 .
Figure 2. Annual dates for bud break, flowering and veraison on-winery for five grapevine varieties in Hainfeld (SW Germany) from 1975-2015.

Figure 4 .
Figure 4. Mean monthly precipitation in Hainfeld (SW Germany) from 1970-2015.Average and standard deviation of fouty years.Nonsignificant differences between sites are indicated by identical letters above the bars and were determined by using Fisher's LSD test at the 5% level after a one-way ANOVA.

Table 1 .
Mean juice density (in ˚Oechsle) and mean juice quantity (in hL•ha −1 ) in five grapevine varieties in Hainfeld (SW Germany) during ten year periods from 1975-2014.
red wine "Pinot Noir" sugar concentrations increased since 1991, with highest r 2

Table 2 .
Mean annual first harvest dates and earlyness of harvest in five grapevine varieties in Hainfeld (SW Germany) during ten year periods from 1975-2014.The "Pinot Noir" vineyard was planted in 1989, which provided a first harvest in 1991.between1975and2015wereregressed, (Figure1(c)), similar results were obtained as from the ANOVA analyses between 1975-1984 and 2005-2014: 25 days for "Silvaner", 30 days for "Müller-Thurgau" and 34 days for "Riesling", and also for "Pinot Noir" a similar number of days was extrapolated (28 days; Table2).

Table 3 .
Mean annual rainfall, mean annual temperatures and Huglin index for Hainfeld (SW Germany) during ten year periods from1970-2014.1970-19741975-1984 1985-1994 1995-2004 2005-2014Average of ten years.*Average of 5 years.LSD denotes Least Significant Difference after one-way ANOVA.Data in the same line with same letter are not significantly different from each other.