Secondary Syphilis in Man : Prozone Effect

Introduction: Syphilis consists in a systemic infect contagious pathology with a chronic character. The etiological agent consists of an anaerobic spirochete bacterium, Treponema pallidum. Laboratory diagnosis can be made through a direct investigation of the etiologic agent or non-treponemal (VDRL) and treponemal (FTA-Abs) serological tests. False-negative results are rare. Objectives: The present report presents a rare case of a man with a secondary syphilis syndrome who was initially undiagnosed due to the prozone effect, since he presented VDRL in low titers, and was later confirmed with a new diluted serum sample and VDRL and FTA-Abs, which were positive. Conclusion: A close look should be given to patients who, although the clinical condition is compatible, complementary exams may not be, and clinical cases should be carefully evaluated so that the patient is not treated late.


Introduction
Syphilis is a systemic infectious disease with chronic evolution, in which is susceptible to outbreaks of syphilitic disease and to periods of restlessness, latency.
The major route of transmission is sexual intercourse, acquired, but it can occur vertically or congenitally, and, in rarer cases, atypical transmission by accident with contaminated material or blood transfusions.When a pregnant woman acquires the infection, there is a potential risk of fetal involvement, which can lead to severe pictures and marked sequelae.During its evolution it develops symptomatic episodes of active disease interspersed with latency periods.Also The disease can affect the organism in a serious and irreversible way, as well as greatly increase the patient's risk of infection with the human immunodeficiency virus (HIV), where its penetration is facilitated by syphilitic lesions.The individual infected with HIV virus presents a more rapid evolution to the acquired immunodeficiency syndrome (AIDS) when there is a concomitant infection with Treponema pallidum [10] [11] [12] [13].
Laboratory diagnosis can be made through the direct etiologic agent investigation in material collected by scraping the lesions or by puncture of lymph nodes.This can be done by dark field microscopy, which is the gold standard for the diagnosis of primary syphilis, or by direct immunofluorescence [14] [15] [16].
Non-treponemal tests (VDRL-Venereal Diseases Research Laboratory) are performed to track infection, but are not as specific and not absolute for this, as they may be positive in other diseases, leading to false positive results, such as In Systemic Lupus Erythematosus, Antiphospholipid Syndrome, Mononucleosis, Malaria, Chagas Disease, Leptospirosis and Tuberculosis.Due to its specificity in congenital syphilis, high sensitivity in adult syphilis, low cost and easy to perform, VDRL is widely used in Brazil, also being a method to evaluate the serological cure [7] [17].
False-negative results are rare, 1% -2%, which makes VDRL a test of high sensitivity and low specificity.This reaction may occur in cases of very recent syphilis infections or because of the prozone effect, which represents an excessive immune response in the formation of antibodies resulting from the infection, with no visualization of the antigen and antibody agglutination.Because of this, an erroneous reading of the VDRL values occurs.To avoid this effect, it is important to always dilute the analyzed serum.Non-treponemal tests are quantified by progressive dilutions, so that the higher the dilution, the higher the antibody concentration.The title values may decrease even without treatment, but only negatives after specific therapy or may remain positive after treatment [7] [17] [18].Journal of Biosciences and Medicines The treponemal tests (FTA-Abs-Fluorescent Treponemal Antibody absorption.MHA-TP-Microhemaglutination Assay for Treponema pallidum. ELISA-Enzyme-Linked Immunosorbent Assay.EQL-Electro chemiluminescence), detect antibodies to Treponema pallidum and thus can establish the diagnosis.These are the first tests that are positive, on average from the first to the second week after infection, and are used to confirm cases of positive VDRL or in cases where it is negative, or in situations where the patient presents characteristic clinical features [19].After initially discarding the dengue diagnosis, hospital admission was requested for investigation, where the patient reported only night chills.During this period laboratory tests were performed, which are represented in Table 1.

Case Report
After the tests collection, the suspicion of prozone effect was raised and confirmed after the serum dilution in a new sample with a result of VDRL 1:32 and FTA-Abs positive, so that the pathology propaedeutic was approached after closure of the diagnosis and the patient being cured from its initial condition.
It is worth noting that the patient was duly explained about his pathological condition and was willing to publish this report, signing the Informed Consent Term, which made it possible to publish it.

Discussion
There is an increase in syphilis incidence, mostly in young men, and especially in those with homo-affective behaviors.One of the reasons for this increase is the change in risk behavior, mainly due to a reduction in the practices of safe sex between them [20].Thus, syphilis appears to be regaining its importance in the landscape of infectious diseases and is currently one of the main predictors of non-use of condoms.It is known that in recent years, the increase in sexual Journal of Biosciences and Medicines promiscuity of both genders, makes syphilis regain its importance even after so long the discovery of penicillin, which effectively treats the infected individual.
In the report there was no previous history of homo-affective habits, but it should be remembered that the unprotected sexual practice transmits the bacterium regardless of gender or age.
Testing should be performed whenever syphilis is suspected at any stage, for all patients with STDs, at risk groups, and in the prenatal routine [21].Although it is not the most effective method for diagnosis, the VDRL is the examination of choice for screening, possibly due to its low cost and high availability.
The interaction between syphilis and AIDS virus is of great importance in world scenario.Both are transmitted mainly through the sexual route and affect populations with similar characteristics.HIV can be transported by secretions and transferred by genital ulcers [22] [23].Serological tests for HIV have been requested in the patient in question, since complete screening is essential in individuals infected with any sexually transmitted disease, especially when it is known that an infection is a door to others, since they have the same means of dissemination.
The diagnosis of syphilis is simple, depends on a good anamnesis, with emphasis on sexual history and analysis of the lesion material to identify the treponema and/or serological tests.Darkfield microscopy is the primary method for diagnosing the primary disease stage, whereas serological tests are the methods of choice for diagnosing secondary, latent and tertiary syphilis, but are not suffi- J.A.S.N., male, 56 years old, white, married, father of three, treasurer, natural and resident of Valença-RJ.He sought care at the Luiz Gioseffi Jannuzzi School Hospital (in Portuguese-HELGJ) of the Faculty of Medicine of Valença, in May 2017.Patient reports that for two months he started with generalized malaise, prostration, adynamia, weight loss of 3 kg from the beginning of the condition and diaphoresis at night.As a history of the current disease, he claimed that for the last twenty days he had started with macular exanthema on the trunk and face, which later spread to the upper and lower limbs with digit oppression disappearance (Figures1-4).

Figure 1 .
Figure 1.Presence of erythematous-macular rash in the thoracic region and abdomen.

Figure 2 .
Figure 2. Presence of erythematous macular rash in the thoracic and dorsal regions.

Figure 3 .
Figure 3. Presence of syphilitic roseola characterized by oval erythematous macules in the plantar region.

Figure 4 .
Figure 4. Presence of syphilitic roseola characterized by oval erythematous macules in the palmar region.