Clinical Correlation between Plasma Homocysteine Level and Coronary Artery Disease in Indian Patients

Objective: The aim of this study was to evaluate correlation between plasma homocysteine and coronary artery disease (CAD) in Indian patients. Methods: This study included 150 patients, 100 subjects in study group with angiographically diagnosed CAD and 50 subjects in control group with a normal coronary angiogram. In the study group, patients were divided into three subgroups viz.: CAD only, CAD with hypertension and CAD with type 2 diabetes mellitus. Plasma homocysteine, lipid profile and other risk factors were compared. Results: Mean homocysteine levels in study group (38.34 ± 15.25 μmol/L) were significantly higher (p < 0.01) than control group (9.41 ± 4.22 μmol/L). No association was found between homocysteine level and conventional risk factors. Furthermore, no significant correlation was found between plasma homocysteine and lipid components in different groups of patients. Conclusion: The study demonstrated that increased levels of homocysteine are independently related to CAD. However, further studies involving a larger sample size will be required to substantiate the findings of the current study.


Introduction
Coronary artery disease (CAD) has become a major public health problem in many countries.According to the World Health Organization, CAD is the most common cause of death throughout the world [1].India has been reported to have the highest prevalence of CAD [2].The major cardiovascular risk factors are high plasma LDL (low-density lipoprotein), low plasma HDL (high-density World Journal of Cardiovascular Diseases lipoprotein), smoking, hypertension, diabetes, obesity and physical inactivity.
An increased level of LDL is related to the development of atherosclerotic cardiovascular disease, which is the primary pathological basis of CAD [3] [4].So, the role of newer emerging risk factors is being recognized especially homocysteine, fibrinogen, and lipoprotein.
Homocysteine is a nonprotein amino acid derived from methionine metabolism.Homocysteine converts to cysteine as a result of trans-sulfuration pathway; each pathway depends on series of biochemical enzymes such as cystathionine β synthase and methylene tetrahydrofolate reductase (MTHFR) as well as on vitamin B12 and folic acid [5].The formation of methylene-tetrahydrofolate is catalyzed by MTHFR enzyme which has an effect on the remethylation of homocysteine and these actions depend on vitamin B12 [6].Molecular deficiency in each of these enzymes can cause hyperhomocysteinemia. Several studies discovered that homocysteine levels are associated with a novel risk factor for CAD and premature atherosclerosis [7].However, the mechanism of atherosclerosis plaques correlated with hyperhomocysteinemia is not clearly stated.Some studies reported that the effect of hyperhomocysteinemia is associated with increased thrombogenicity, an increase of platelet aggregation, reduction of protein C activation, oxidative damage of LDL, and endothelial dysfunction [8].
Hyperhomocysteinemia may lead to enhancement adverse effects of risk factors, lipoprotein metabolism, and development of inflammation [9].The factors which influence the level of homocysteine may vary significantly in a population with age, genetics, and nutrition [10].Elevated homocysteine levels are associated with some factor including increasing age, male sex, smoking, coffee consumption, high blood pressure, lipid profile, high creatinine and improper diet [7].Low folate and vitamin B12 are related with elevated homocysteine levels [11].The incidence of homocysteine levels is altered in ethnic groups due to different genetic conditions, nutritional factors (vitamin deficiency and folic acid deficiency) and lifestyle behaviors [12].Therefore, the present study was undertaken to examine a possible relationship between plasma homocysteine level and coronary artery disease in Indian patients.

Study Design
The present study was conducted for a period of 1 year from October 2015 and October 2016 in the cardiology department, District hospital Palakkad, Kerala.
The patients who subsequently underwent first elective coronary angiography and plasma homocysteine assessments were considered in this study.The present study included 150 patients, 100 subjects in study group with angiographically diagnosed CAD and 50 subjects in control with a normal coronary angiogram.Subjects in the study group were divided into three subgroups viz.: CAD only, CAD with hypertension and CAD with type 2 diabetes mellitus (DM).
Elective coronary angiograms were done at least two weeks following an acute World Journal of Cardiovascular Diseases coronary event.Indications for coronary angiography were: 1) evaluation of ischemic heart disease or cardiomyopathy and 2) before coronary artery bypass grafting 3) aortic or valvular heart disease surgery or further preoperative investigation in patients with family history of CAD and positive noninvasive test results.Written informed consent was procured from every patient.Angiographic SYNTAX scorings were performed by observers blinded to plasma homocysteine measurements.The inclusion criteria for the study group were: 100 subject having ≥50% luminal narrowing in arteries, having ≥1.5 mm width in coronary artery or its major branch.50 subjects in control were matched by age and sex individuals who had a normal coronary angiogram.Although the inclusion criteria included all age group, patients were aged above 37 years, absence of any acute disease and informed consent granted.
Patients having hypertensive emergencies, hepatic disease, renal disease, stroke, hypothyroidism, pregnancy and taking any other drugs like methotrexate, carbamaze-pine, phenytoin, theophylline or any form of vitamin supplementation were excluded from this study.All patients undergoing coronary angiography immediately following acute coronary events and all hemodynamically unstable patients were excluded from the study.
After obtaining informed consent, selected cases were subjected to detailed history, laboratory test and imaging studies like ECG, fundoscopy, Echocardiography, blood sugar, hemoglobin A 1 c, lipid profile, complete blood count and other routine examinations.

Estimation of Plasma Homocysteine
Plasma homocysteine was estimated using ADVIA Centaur Homocysteine Assay (ADVIA Centaur XP Immunoassay System) which is a one-step competitive immunoassay employing direct Chemiluminescence to measure total homocysteine in EDTA plasma or serum quantitatively.

Statistical Analysis
Statistical analysis was performed using Student's t-test for paired samples using SPSS v.21.0.Values were expressed as a mean ± standard deviation or as percentages.A p value < 0.05 was considered statistically significant.

Results
The age of the patients ranges between 37 and 77 years.Among 150 patients, 105 (70%) were males, and 45 (30%) were females.In the study group, 75 cases (75%) were males and 25 cases (25%) were females (Table 1).Plasma homocysteine levels were significantly higher in females than males in both the study and control groups (p < 0.001).Plasma homocysteine level in study group (38.34 ± 15.25) was significantly higher than a control group (9.The lipid profile changes in control and subgroups of patients are shown Table 4.The levels of total cholesterol, triglyceride, and LDL cholesterol were significantly higher in group-II (p < 0.001) as compared with control group.
Moreover, group-II patients had significantly decreased (p < 0.05) HDL cholesterol level than control group.Group-I showed significant variation (p < 0.05) in the levels of total cholesterol, and LDL cholesterol as compared with control.
Also, group-III showed a significant increase (p < 0.05) in triglyceride level as compared with control.The correlations between homocysteine and lipid profile parameters are shown "r" (coefficient of correlation) and "p" value in different groups of patients (Table 5).There was no significant correlation between plasma homocysteine and lipid components in different group patients.The plasma homocysteine levels are represented in Figure 1.

Discussion
Homocysteine has been projected as a novel risk factor for CAD [13].Some researchers embarked upon the mission to show plasma homocysteine as an independent risk factor for CAD.Some studies reported that correlation between high plasma homocysteine and atherothrombotic vascular disease in patients [14] [15] [16].In this study, we demonstrated the relationship between plasma World Journal of Cardiovascular Diseases homocysteine level and CAD in Indian patients.Most prospective and retrospective studies have demonstrated that high homocysteine is an independent risk factor for CAD [7].Previous studies have shown that high rates of CAD in Asian Indians are associated by high prevalence of conventional risk factors such as hypercholesterolemia, hypertension, and smoking [4].Furthermore, the find- In a current study, we found no significant correlation between plasma homocysteine level and other conventional risk factors of CAD (hypertension, diabetes mellitus, smoking, and alcohol consumers).These results were similar to those reported in Deepa et al. study [24].In our study, total serum cholesterol, triglycerides, LDL were significantly higher (p < 0.001) and HDL level significantly lower in the study group compared to control and specifically in group II (CAD with hypertension).We calculated Pearson' correlation coefficient and World Journal of Cardiovascular Diseases "p" value in different groups for plasma homocysteine levels with lipid profile.
Furthermore, there was no significant association between plasma homocysteine and lipid profile.A similar finding has been reported from Puri et al. [4].This variation may be due to small sample size confounding factors in our study.
Higher plasma homocysteine values in our study group indicate that our population subgroups are deficient in folate levels and require folic acid supplementation.This intervention may decrease the premature incidence of CAD and reduce the mortality rate of the population.

Conclusion
This study indicates that elevated plasma homocysteine is an independent risk factor associated with CAD.Further studies involving a larger sample size will be required to investigate the benefits from vitamin administration in patients with increased homocysteine levels to prevent the premature incidence of CAD.

Figure 1 .
Figure 1.Plasma homocysteine levels in study and control group.

Table 2 .
Comparison of plasma homocysteine level in different study and control group.

Table 3 .
Characteristics of homocysteine in patients with conventional risk factors.
thermore, the mean homocysteine level of alcoholics and non-alcoholics are 39.327 ± 11.72 and 40.860 ± 15.28, respectively.There was no significant correlation between the average of two groups (p = 0.21).

Table 4 .
Lipid profile parameters in different study and control group.

Table 5 .
Correlation between homocysteine and lipid profile parameters