Clinical Pathological Analysis in 104 Ductal Breast Cancer Cases

Ductal breast cancer is a clinically heterogeneous disease; we investigate the correlation between tumor size, comedo necrosis, number of cell mitoses and axillary lymph node metastasis in ductal breast cancer. In this study, 104 ductal breast cancer specimens were collected and divided into 3 groups: T1 group (tumor size ≤ 2 cm), T2 group (2 < tumor size ≤ 5 cm) and T3 group (tumor size > 5.0 cm). Among those specimens, 95 cases were diagnosed with invasive ducted carcinoma, and 9 cases were ductal carcinoma in-situ (DCIS). Results show that T3 group has a higher rate of axillary lymph node metastasis than T2 group and T1 group; T2 group has a higher rate of lymph node metastasis than T1 group. The patients with the number of cell mitoses (≥10) were also has a higher rate of axillary lymph node metastasis (P = 0.0139) than the patients with the number of cell mitoses (<10). No significance was found between comedo necrosis lesions and axillary lymph node metastasis, though the frequency of comedo necrosis lesions in patients with axillary lymph node metastasis was higher than those in non-metastatic patients. It is concluded that the tumor size and the number of cell mitoses were risk factors for axillary lymph node involvement in ductal breast cancer.


Introduction
Breast cancer is the most common malignant tumor among women, most of which are confirmed by pathological examination. In this study, 104 cases of ductal breast cancer specimens were collected. We analyzed and discussed the 3. Methods

Hematoxylin and Eosin Staining
The original tumours have been fixed in 4% formaldehyde for 24 h, and then embedded in paraffin. Paraffin blocks were made of 3 -4 μm thick sections, after routine hematoxylin and eosin staining; the pathologist observed the histopathological changes and classified the grade under optical microscope.

Classification of Samples
The largest diameter size of gross tumor as the measure standards, the samples were divided into three groups: T1 group (tumor size ≤ 2 cm), T2 group (2 < tumor size ≤ 5 cm) and T3 group (tumor size > 5.0 cm). According to Bloom-Richardson histological grading system, we divided infiltrative breast cancer into I, II, and III grade.

Statistical Analyses
Analysis was performed using GraphPad software, statistical analysis was performed by Fisher's exact test, P < 0.05 was considered to indicate a statistically significant difference.
The relationship between comedo necrosis, lymph node metastasis and stage of breast cancer. In the 50 breast cancer cases who had lymph node metastasis, there were 39 cases accompanied comedo necrosis, while 21 cases with comedo necrosis among 53 cases with no lymph node metastasis. Although the rate of lymph node metastasis patients accompanied comedo necrosis (39/103) was higher than the rate of without lymph node metastatic rate (21/103), there was no significant difference (P > 0.05). Results also shown that, with the increase of tumor clinical grade, the number of samples with comedo necrosis also gradually increased, but there was no significant difference ( Table 2).
The relationship between the number of cell mitoses and axillary lymph node metastasis. Results are shown in table 3. In the high-power fields (×400), we count the number of cell mitoses. 104 cases of resected specimens with invasive breast cancer were divided into two groups: 0 -9 mitoses in high-power fields as a group I, ≥10 mitoses as a group II. We analyzed the difference between those node metastasis in group II is more than that of group I. The difference was statistically significant (P < 0.05).

Discussion
Ductal breast cancer is a clinically heterogeneous disease. About 10% to 15% of breast cancer patients have distant metastases within 2 years, which significantly reduced the 5 year survival rate [1]. Staging, Tumor size and lymph node metastasis are important prognostic factors for breast cancer metastasis, and in those factors, lymph node metastasis is the most important effect on ductal breast cancer prognosis.
We analyzed the pathological diagnoses on 104 cases of ductal breast cancer.
The results were shown in Figure 2. The peak of the age on breast cancer is still more than 40 years old. But it is worthy of mentioning that within 12 patients less than 40 years old, 7 (58.33%) had axillary lymph node metastases.
The youngest was 24 years old, who was diagnosed as invasive ductal carcinoma, axillary lymph node metastasis is positive. Therefore, it is suggested that in any age group of women, as long as the breast tumor is found, it should be pay great attention to do a physical check, so to improve the early diagnosis rate, and do the best measures on improving the survival of breast cancer. Table 1 shows the relationship between the breast cancer tumor size and axillary lymph node metastasis, the larger the tumor size the higher rate of axillary lymph node metastasis.
Breast cancer axillary lymph node metastasis rate was 25.7% in T1 Group, 51.8% in T2 group, and in T3 group, the axillary lymph node metastasis rate rose to 92.3%. There were statistically significant differences between different groups compassion. Recently, study confirmed, monitoring the prognosis marker on breast cancer metastasis has positive correlation with the tumor size [2]. For example, there is correction between high serum concentration (more than or equal to 2 mu/ml) ck19-2g2 and the tumor size (more than or equal to 2 cm).
But in the early stage of breast cancer, tumors often cannot be checked out. So, research on relative tumor marker has a great value of diagnosing breast cancer.
Reports have revealed comedo necrosis is a valuable predictive factor of breast tumor recurrence risk factors [3]. Comedo necrosis also could be seen in small breast infiltrating cancer such as ductal carcinoma in situ, which has high risk of axillary lymph node metastasis [4]. And the prognosis of comedo necrosis ductal carcinoma in situ was more poor than the non-comedo necrosis ductal carcinoma in situ [5] [6]. Within the 104 cases of breast cancer tissues, we got 9 cases of patients with carcinoma in situ. Therefore, we analyze the relationship among the comedo necrosis, stage and axillary lymph node metastasis. Our results shown there was no statistically significant difference between comedo necrosis and lymph node metastasis ( Table 2), but there still was 39/103 of lymph node metastasis cases with comedo necrosis, the ratio was higher than no lymph node metastasis cases (21/103). Then we analyzed comedo necrosis combined with lymph node metastasis cases in different breast cancer stage. In stage I there were 13.3% cases had comedo necrosis combined with lymph node metastasis, while in stage II and III, the ratio was34.4% and 45.8% respectively (Table 3).
Data revealed that perhaps higher tumor stage in ductal breast cancer has higher ratio of comedo necrosis lesions and lymph node metastasis, however, there is no significant difference between the statistical results, which is most likely due to the number of limited cases.
Some scholars believe that cell mitoses represent the biological behavior of the tumor and can make greater value and clinical significance [7]. With the application of computer automatic image analysis technology in the detection of prognostic markers in breast cancer [8] [9] [10] [11] [12], the counting method of nuclear fission image now becomes more and more accurately. In this study, we watched the relationship between number of cell mitoses and axillary lymph node metastasis. Data shown that the group (≥10 cell mitoses) showed significantly higher patient with axillary lymph node metastasis compared to the group (0 -9 cell mitoses) (P = 0.0100).
Every 100 thousand women in the United States from 25 to 39 years of age, the incidence of breast cancer increased from 1.53% in 2009 to 2.90% in 1976 [13]. In recent years, the incidence of female breast cancer in China is also rising, and the disease incidence showed younger trend. Lymph node metastasis may reflect the biologic behavior of the tumor, to analysis the pathological features of breast cancer, and to explore the related factors of axillary lymph node metastasis, have important significance on the treatment and prognosis of breast cancer. If breast cancer recurrence, the main causes of death were axillary lymph node metastases [14]. The scope and number of the lymph node dissection can affect the treatment and prognosis of patients [15]. Through initial analysis of breast n: number of cases. The number of patients with axillary lymph node metastasis in group II was more than that of group I; the difference was statistically significant (P = 0.0139; P < 0.05).
cancer pathological type, we believe that breast cancer with axillary lymph node metastasis is closely related to the tumor size, and the number of cell mitoses is closely related to axillary lymph node metastasis too. Therefore, more attention should be paid to comedo necrosis during clinical process.