Integrated Nutrient Management of Safflower ( Carthamus tinctorius L . ) under Rainfed Conditions

Optimistic and sustainable supply of soil available nutrients to crop plants enhances productivity. Integrated nutrient management (INM) approach can improve soil fertility on long term basis. The present study was conducted to determine effects of INM on quantitative and qualitative characters of two Safflower (Carthamus tinctorius L.) cultivars “Thori-78 and Leed-00”. Five treatments using different composition of poultry litter, farm yard manure, nitrogen and phosphorous fertilizers with recommended dose as a control measure were replicated thrice in randomized complete design. The results of field trial depicted maximum plant height (174.6 cm), number of heads plant (42.67), number of seeds head (59.0), thousand seed weight (42.26 g), biological yield (3089 Kg∙ha) and seed yield (455.2 Kg∙ha) recorded from combined application of FYM @ 2 t∙haand Half (N-P) (soil application) (T5) in Genotype “Leed-00” which was statistically different from all other treatments. Thori-78 also showed increase in yield and yield components under the same treatment (T5) i.e.2 t∙ha FYM and Half (N-P).No effect of INM was found on fatty acid composition of safflower cultivars. The correlation coefficients illustrated positive and significant association of seed yield with plant height (0.89), number of heads pod (0.86) and number of seeds head (0.83) as a result of application of selected treatment. These results demonstrated the significance of INM in safflower yield improvement under rainfed conditions.


Introduction
Safflower can be cultivated as an oil seed crop under poor environmental conditions to increase the edible oil sources [1].Safflower seed contains 35% oil contents and occupies a unique position among oil seed crops due to the high linoleic acid content of its seed oil [2].
Safflower is multipurpose crop with unexplored potential and worldwide adaptability.Safflower acreage and production has been witnessing wide fluctuations around the world.Low productivity of safflower is a serious concern for sustainable crop development.A negative growth rate in terms of area, production and productivity has been recorded around the world, especially in Pakistan.Safflower is a high value crop due to its immense potential for use in domestic, medicinal and commercial use [3].
Safflower oil contains unsaturated fatty acids including linoleic, oleic and saturated fatty acids as palmitic and stearic acid.It is one of the richest sources of linoleic acid among the commercial oils [4].Safflower seed oil contains about 71% -75% linoleic acid, 16% -20% oleic acid, 6% -8% palmitic acid, and 2% -3% stearic acid [5].This low level stearic and palmitic acids has madesaf flower oil appealing to consumers, initially as quick-drying oil, more recently as edible oil with the highest linoleic acid.
Plant nutrients in soil whether naturally endowed or artificially maintained are major determinants of the success or failure of a crop production system.Proper soil fertility management is of prime importance to increase crop production.Post-green revolution period has seen a tremendous rise in the use of fertilizers for enhancing productivity of field crops.This has resulted not only the deterioration of land resources but also contaminating the environment at the same time raising the cost of production [6].
Integrated nutrient management is an emerging method for sustainable agricultural production that poses the least threat to the environment disruption.
Blending of inorganic fertilizer is a vital component of integrated nutrient management, yet it is often not available to farmers in developing nations [7].Use of organic manures, apart from improving physical and biological properties of soil, facilitates efficient utilization of chemical fertilizers [8].Combined use of organic and synthetic fertilizers in appropriate ratio is advantageous in escalating the crop yield and sustaining soil health [9].Morphological and physiological performance of crop plants in response to INM can help in evaluating the suitability of the particular INM technique as no systematic study has been conducted on this particular crop.The present investigation was therefore undertaken to observe the effects of different integrated nutrient management combinations on performance of yield and oil quality of safflower cultivars under rainfed conditions.

Site Description
The response of safflower to integrated nutrient management combinations were studied at Research Farm, Arid Agriculture University, Chakwal Road (32.93˚N, 72.86˚E) during the Rabi season, 2010-2011 under rainfed conditions.
Previously fallowed field was prepared by ploughing three times with tractor mounted cultivator and planking with last ploughing.Sowing was done with hand drill on November 22, 2010 with the seed rate of 15 kg•ha −1 .The plot size was 5 × 3.6 m 2 .Row to row distance was 60 cm and plant to plant distance was maintained at 10 cm.Climatic data during the experimental period at the experimental farm is presented in Figure 1.

Measurements
Data were recorded for quantitative characters of safflower like plant height (cm), number of heads plant −1 , number of seeds head −1 , thousand seed weight (g), biological yield (Kg/ha) and seed yield (Kg/ha) at harvesting, done on 26 th  April 2011.Data recorded for different parameters were subjected to statistical analysis using R software by computing analysis of variance.

Fatty Acid Composition
Fatty acid composition of seed samples (stearic, palmitic, oleicand linoleic) was determined through gas chromatograph by methylating the fatty acyl-esters in the oil.The composition and identification of fatty acid was carried out by the relative retention times of the standards [12] [13].
The means were analyzed and compared at p ≤ 0.05 by F-protected least significant difference test (LSD).The simple correlation coefficients (r) of grain yield with other yield components were also worked out according to [14].

Plant Height (cm)
The mean data regarding plant height of safflower (Table 2) showed that it was significantly affected by different treatments.Maximum plant height (170.0 cm) was recorded with [Half (N-P) + FYM @2 t•ha −1 (T 5 )] followed by T 3 (166.9cm) and T 4 (166.0cm) while minimum plant height (142.6 cm) was observed in T 1 (Control).The plants with maximum height (174.6 cm) were observed for cultivar Leed-00, whereas the minimum height (134.0 cm) was recorded for local cultivar Thori-78.Significant variation in plant height might be due to genetic factor as the cultivars were of different origin.Similar results were obtained by [15] who concluded that combined application of organic and inorganic fertilizer significantly increased the plant height.These results are in conformity with [16] [17] who stated that differences among the plant height might be due to nutrient status and availability thus plants responded to the treatments according to the nutrient supply.among the interaction of cultivars and treatments.These results are also in line with the findings of [15] [18] who reported that organic fertilizers ensures better availability of nutrients which produced better crop development and also number of pods per plant.

Number of Seeds Head −1
There were significant differences among the treatments for number of seeds per head (Table 2).The maximum (48.67) seeds were recorded when FYM was applied in combination with NP, whereas, the minimum (28.33) seeds per head were observed in T 1 (control), rest of treatments were statistically different with each other.Leed-00 produced maximum (59.0) and Thori-78 minimum (38.33).Maximum number of seeds per head for Leed-00 andThori-78 were recorded (59.00, 38.33) in T5 followed by T3.Tested cultivars were statistically different for number of seeds per head which might be due to their different genetic makeup and their response to available soil nutrients [19] [20] also stated significant increase in the number of seeds in safflower as result of INM.The results are also in the line with [20] [21] who reported integrated use of organic and inorganic fertilizers significantly increased the number of seeds per head.

Thousand Seed Weight (g)
The data regarding 1000-seed weight (Table 2) showed maximum value (42.26 g) from treatment (half (N-P) + FYM @ 2 ton•ha −1 ) (T 5 ) followed by T 3 (40.32g) and T 4 (40.04 g), while the minimum seed weight (32.55 g) was under T 1 (control), furthermore, T 2 and T 6 remained non-significant.Interactive effects for treatments verses cultivars were statistically significant for thousand seed weight.The maximum 1000-seed weight (42.26 g) was observed in Leed-00 by T 5 .whileThori-78 showed (42.08 g) 1000-seed weight by the same treatment (T 5 ).Superior performance of safflower cultivars could be attributed to the more availability of nutrients from farm yard manure applied in combination with inorganic fertilizers [22] (Kubsad et al., 2008).Similar trend was found in the findings of [15] [23] who observed significant differences for 1000 seed weight in safflower by the combined application of synthetic and farm yard manure fertilizers.

Biological Yield (Kg/ha)
Biological yield is a combination of seed yield and straw yield and is direct index of photosynthetic machinery.It becomes more important for multipurpose crop like safflower as the high biological yield will not only provide good seed yield but also more dry matter can be used for fodder purpose.The safflower biological yield increased significantly as a result of addition of chemical fertilizers and organic manure compared to control (Table 2).Maximum biological yield (3034 Kg•ha −1 ) was produced by T 5 .Data in (Table 3) showed that cultivar Leed-00 produced the maximum (3089 kg/ha) biological yield with the combination of FYM and half (N-P) fertilizers.However, cultivar Thori-78 produced maximum (2978 kg/ha) biological yield by treatment T 5 which was at par with T 4 and T 3 .Maximum biological yields with integration of organic and inorganic fertilizers in safflower might be due to the increased availability of nutrients.Our results are in agreement with [24].Moreover, [25] were of the opinion that balanced application of organic and inorganic fertilizer application has significant impact on stalk yield of safflower

Seed Yield (Kg/ha)
The maximum seed yield (435.7 Kg•ha −1 ) was obtained by T 5 , followed by T 3 (402.6Kg•ha −1 ) and T 4 (383.3Kg•ha −1 ), while, the minimum (319.8Kg•ha −1 ) seed yield was observed in T 1 (control).There were non-significant differences between the cultivars for seed yield.The overall performance of Leed-00 was better than Thori-78.The maximum (455.2Kg•ha −1 ) seed yield was observed in cultivar for Leed-00 by T 5 and maximum (416.2Kg•ha −1 ) seed yield was recorded in cultivar Thori-78 by T 5 .Premier seed yield in tested cultivars of safflower might be due to more availability of nitrogen at vegetative growth stages as combination of inorganic source of nitrogen and farm yard manure [26] [27].Flourished vegetative growth ultimately performed efficiently as stronger source and translocated more photosynthates resulting in higher seed yield.The results are in the agreement with [14] who concluded that integrated use of organic and inorganic fertilizer performed better over their sole application for the final seed yields of safflower.[28] also concluded that farm yard manure along with recommended dose of fertilizers significantly improved seed yield.

Correlation
Correlation coefficients among different pair of agronomic traits were calculated to find out relationship among various characters studied.The values of correlation coefficient are presented in (Table 4).It can be observed that seed yield plant −1 was positively correlated with plant height, number of heads plant −1 , number of seeds head −1 and 1000-grain weight that revealed importance of these characters in determining yield.Positive relationship between seed yield, plant height, number of heads plant −1 and number of seeds head −1 obtained in this study confirmed the findings of [29] [30].The height positive correlation coefficient was determined between seed yield plant −1 and plant height (r = 0.890) and seed yield and number of head plant −1 (r = 0.868).These results are in accordance with the findings of [31] who observed highly positive correlation between seed yield per plant and head (capitulum) number.Saf-flower is considered to be one of the highest quality vegetable oils and consists of mainly palmitic, stearic, oleic and linoleic acids [32].Significant difference of the contents of these fatty acids among tested safflower cultivars seems to be a genetic factor as there is no effect of integrated nutrient management was observed.[33] also stated that qualitative characters of a crop depend on seed related factors like genetic makeup.

Conclusion
The present study concluded that there is scope of increasing crop yields through use of integrated nutrient management.It is also important in maintaining soil fertility status.Exploitation of safflower as a useful nutritional, medicinal and industrial source may prove a key intervention to increase the popularity of this crop and for restoring its status as an important oilseed crop.

Figure 1 .
Figure 1.Climatic data during the experimental period at the experimental farm from November, 2010 to April, 2011.

Figure 2
Figure 2 represents the significant differences for oil contents within the treatments and between the varieties Lead and Thori-78.Fatty acids contents like palmitic, stearic, oleic and linoleic acids are very decisive in determining the

Figure 2 .
Figure 2. Effects of Integrated Nutrient Management (INM) treatments on oil yield, oil, palmitic, stearic, oleic and linoleic acids contents of safflower.

Table 1 .
(a) Soil characteristics of the experimental site prior to sowing; (b) Characteristics of Farmyard Manure and Poultry Manure used as Organic Fertilizer in the Experiment.

Table 2 .
Response of Safflower to integrated nutrient management.Data presented in Table2depicted maximum (39.50) number of heads plant −1 from T 5 followed by T 3 (32.0)and T 4 (28.67) which was statistically significant compared to T 1 (control), T 2 and T 6 where minimum (20.83, 23.00 and 24.50) heads were observed.The cultivars Leed-00 and Thori-78 recorded the maxi- mum (42.67) and (36.33) heads per plant with T 5 , while T 1 showed minimum count for number of heads per plant i.e. (21.00 and 20.67) Table3.Better availability of nutrients may result in better crop growth rate and ultimately more number of heads in safflower.Statistically significant differences were observed

Table 3 .
Response of safflower cultivars to integrated nutrient management.

Table 4 .
Correlation co-efficient for mean quantitative traits of safflower cultivars.