Darwinian Selection in Prostate Cancer and Medical Treatment

Organisms evolved into different species to adapt to the environment according to the laws of Darwinian evolution. In a single life, prostate cancer cells can also evolve into tumor stem cells to adapt to the microenvironment, such as different chemotherapeutic drugs. These cancer cells become an unrestricted growth group relatively independent of the individual. The present review attempts to establish evidence that prostate cancer cells may survive by hormonotherapy and chemotherapy by gene amplification, mutation, and alternative splicing. Simultaneously, novel treatment strategies have been cited and evaluated, avoiding the resistance mechanisms.


Introduction
In Europe and the USA, prostate cancer (PCa) is one of the most common malignant tumors and the second leading cause of cancer-related deaths in men.
PCa is primarily an androgen-dependent tumor that is treated using androgen deprivation therapy (ADT).The androgen receptor (AR) is a key driver molecule leading to the occurrence and progression of PCa, even in the stage of castration-resistant PCa (CRPC).The AR is a multi-domain, ligand-inducible nuclear transcription factor encoded at Xq11-Xq12.It is encoded by eight exons and has four structural domains: the N-terminal transcriptional activation domain (exon 1), the DNA-binding domain (DBD) (exons 2 -3), a hinge region (exons 3 -4), and the C-terminal ligand-binding domain (LBD) (exons 4 -8).
The AR may be activated by androgen through binding to LBD of the receptor , then the AR is internalized to the nucleus and interacts with target genes through its conserved DBD binding to androgen-responsive elements (ARE).

C. J. Song
Moreover, AR complex regulates the transcription of its target genes with the help of coactivator and corepressor proteins.
The AR signaling axis (Figure 1, from WikiPathways) is the core of all stages of the PCa pathophysiology and serves as the primary target for endocrine-based therapy [1].The current treatments for PCa involve inhibitors of androgen production and antiandrogens blocking the interaction between ligands with AR-LBD [2].

Darwinian Selection in the Therapies for Prostate Cancer
"Natural selection, survival of the fittest" dogma states that species evolve and develop from junior to senior, from simple to complex, from less to more through heredity, mutation, and natural selection.The principal mechanism of evolution, natural selection, has been confirmed by several observations and experiments.Natural selection is an adaptive evolution, i.e., if the variation of species is suitable for the changing environment, then the species will emerge victorious in the struggle for survival; however, if the variation of species is unsuitable for the survival conditions, then it tends to perish.
Darwin's natural selection mainly refers to organisms in the natural world.
Nevertheless, the survival mechanism also exists in an independent individual, and similar to Darwin's theory, it constantly affects human health and life, for example, the disposition between PCa and clinical treatments.Although ADT is originally effective in the most PCa patients, the effectiveness is short-lived.Most PCas will progress to the lethal castration-resistant and metastatic phenotype.Genomic alteration, clonal selection, and evolution of the tumor microenvironment will contribute towards unique physiological characteristics under selection pressure.These pressures contain the chemotherapeutic drugs applied in the clinical setting, and the exposures of PCa cells to hormonal stimuli in the tumor microenvironment.AR is a key driver molecule to take part in PCa development and progression, response to initial ADT, and subsequent resistance to chemotherapeutics.These malignant cells undergo several adaptive changes to ensure persistent androgen signaling.
For a long time, docetaxel was the only drug to treat CRPC.Recently, new drugs for CRPC based on different mechanisms have become available, including CYP17A1 inhibitors reducing androgen production and antiandrogens targeting AR signaling.Scientists have found two types of antiandrogens, including the steroidal antiandrogens and the non-steroidal antiandrogens.Among them, the steroidal antiandrogens are limited in clinical use because of their severe side effects and low efficacy.While the non-steroidal antiandrogens include the firstgeneration antiandrogens: nilutamide, bicalutamide, flutamide, and the secondgeneration: enzalutamide and ARN509, etc.These non-steroidal antiandrogens have been widely used clinically due to avoiding the constraints of the steroidal antiandrogens.However, after an initial effective response, approximately 50% of the PCa patients recurred under the treatment of antiandrogens [3].
Several major resistance pathways focus on androgen signaling, including AR overexpression and amplification, expression of AR mutants inducing promiscuity, response to non-androgen ligands, and constitutively active AR splice variants (AR-Vs) with ligand-independent activity.Intratumoral de novo biosynthesis of androgen and conversion of weak adrenal androgen into strong dihydrotestosterone (DHT) may also contribute to ligand-dependent AR activation.

AR Amplification, Mutants, and Variants
One of the most common events observed in PCa progressing from hormone-sensitive to castration-resistant form is AR gene amplification, occurring in 45% [4].In addition, a correlation between elevated expression of full-length AR or AR copy number gains resistant to second-generation antiandrogens has been documented [5].This could be explained by an adaptive pharmacokinetic change, in which, the androgen is unable to achieve sufficient concentrations within the tumor, and thus, the increased intratumoral steroidogenesis overcomes the inhibitory effects of antiandrogens [6].
The mutations of AR are rare in untreated PCa patients and take place in approximately 15% of CRPC patients.Notably, the mutations of AR in PCa under the therapeutic pressure are responsible for such phenomena that conversion of the antiandrogens into AR agonists and progress of the early PCa to its lethal state, CRPC.Table 1 shows the confirmed AR mutants and the related drug resistance.
Over the last few years, along with AR mutations, constitutively active AR splice variants also promote the progression of PCa.As shown in Figure 2, Lu et al. decoded the AR splice variant transcripts [7].Among them, two most com- mon AR-Vs, AR-V7 and AR-v567es, were detected in 67.53% and 29.87% of CRPC samples, respectively [8].AR-V7 lacking the AR LBD still retains the ability to activate transcriptions of its target genes.Antonarakis et al. found that AR-V7 conferred resistance to abiraterone and enzalutamide in metastatic CRPC, not to taxane [4] and cabazitaxel [9].Constant expression of AR-v567es in the benign prostate tissues may induce epithelial hyperplasia and invasive adenocarcinoma [10].

Tumor Microenvironment
In hormone-sensitive, localized PCa, intraprostatic androgen has been found to categorize by ~75% after the initial treatment, with residual androgen sufficient to drive the transcriptions of AR target genes [11].However, in castration-resistant tumors, the elevated tumor androgen is characterized by the capability to activate the expression of AR and its target genes, by steroid synthesis, enzyme alterations that may increase de novo androgen level, or by the absorption of circulating hormone substrates and adrenal androgens into the tumor microenvironment [4] [12].

New Drugs
AR is encoded by the most complex gene in the nuclear steroid receptors, consisting of 4 domains (NTD, DBD, a hinge region, and LBD).An unsophisticated model of AR signaling includes 6 steps: 1) interaction between androgen and AR LBD; 2) dissociation of heat shock proteins from AR; 3) dimerization of AR and nuclear transport; 4) combination of AR and ARE located in the promoters of its target genes; 5) recruitment of AR co-activators to form AR complex; and 6) transcription and expression of AR target genes.In addition, AR phosphorylation, degradation, and interaction with co-regulators also play crucial roles in modulating the AR signaling pathway.Given the above criteria,  investigators have been pursuing effective therapies for PCa, some already successful in the clinical application inducing drug resistance, which in turn necessitates the development of new drugs and clinical trials.

Specific Drugs Binding with Different AR Domains
The antiandrogens: enzalutamide, bicalutamide, and flutamide, primarily target the LBD of AR [13].However, the LBD is non-conservative and susceptible to mutations; PCa cells might survive as AR signaling could be activated after mutation.The novel chemotherapeutic drugs targeting the conservative domains of AR, combat PCa cells that alter to integrate with the tumor microenvironment.

Inhibitors of AR Transcriptional Activity, Nuclear Translocation, and Degradation
PCa cells survive from various therapies through AR amplification, mutation, splice variants, and elevated androgen concentration in the microenvironment.
Scientists continually strive to find advanced treatments for PCa; for example, blocking AR transcription, interfering with AR nuclear localization, and promoting AR degradation that blocks the initiation of AR signaling pathways.
miRNA binding sites are found within the AR 3'-untranslated region (UTR) or coding regions.The miR-30 family members are direct AR inhibitors [19].
miR-124, as a tumor suppressor, limits the growth of PCa by targeting the AR transcripts and directly downregulating AR-V4 and AR-V7 [20].miR-212 downregulates hnRNPH1 expression which decreases the expression of AR and AR-V7 in PCa cells [21].PCGEM1 is a long non-coding RNA (lncRNA) that is often upregulated in PCa.Rescue experiments demonstrate that the re-expression of PCGEM1 lead to the upregulation of AR-V3 that is responsible for castration resistance [22].Lin28 enhances the expression of AR-V7 by upregulating splicing factor hnRNPA1, and promotes the development of drug resistance [23].
The taxanes, cabazitaxel, and docetaxel, function at 3 aspects : stabilizing the microtubules, inhibiting the cell mitosis, and inducing apoptosis.They inhibit AR activity by interfering with AR nuclear translocation [24].Taxanes effectively lessen the transcriptional activity of wild-type AR, but not those of AR-Vs lacking the microtubule-binding domain.In addition, ICRF187 and ICRF193, DNA topoisomerase II (Topo II) inhibitors, decrease transcription of the wild-type AR, mutant ARs (W741C and F876L), and AR-V7, reduced the AR recruitment to promoters of its target genes, and obstruct the nuclear localization [25].
CH5137291, an AR antagonist, inhibited the nuclear translocation of the wildtype AR, mutant ARs (W741C and T877A) [26].Moreover, ODM-201, a nonsteroidal oral AR inhibitor, potently inhibits the interaction between androgen and AR LBD, and nuclear localization of AR.Importantly, ODM-201 is an effective antagonist for AR F876Lresistant to enzalutamide and ARN-509, and AR mutants (W741L, F876L, and T877A) resistant to bicalutamide and hydroxyflutamide.ODM-201 is currently in a phase 3 trial in CRPC [27].The Src-Abl dual kinase inhibitor, PD180970, decreases AR-V7 expression, nuclear translocation, and androgen-independent transcription of target genes [28].A thiohydantoin derivative, BAY 1024767, exhibits strong repressive activity to the wild-type AR and nine AR mutants by promoting nuclear localization in the absence of R1881 [29].
Galeterone, an AR antagonist, may inhibit CYP17 lyase activity and ubiquitylate AR protein.Galeterone selectively enhances the degradation of AR T878A.
At low micromolar concentrations, it enhances AR protein degradation, whereas, at high concentrations, it could induce an endoplasmic reticulum (ER) stress response and may decrease AR translation.Based on results of the phase I/II trial, a phase III study on galeterone in CRPC is planned [30].Niclosamide, an anti-helminthic drug, potentially suppresses the AR-V7 expression by enhancing its degradation, a phase I trial testing enzalutamide plus niclosamide in CRPC is being pursued [4] [31].In addition, C-4 heteroaryl 13-cis-retinamides inhibit the AR transcriptional activity through degradation of the wild-type AR and AR-V7 in PCa cells [32].ASC-J9, an AR degradation enhancer, may degrade both the wild-type AR and AR-V3 [33].

AR Signaling Inhibitors
In addition to truncating the initiation of AR signaling pathways, the scientists also design the other drugs targeting multiple key sites upstream and downstream of AR signaling, in order to further to reduce the AR-dependent signaling pathways for the development of PCa.

Co-Activator Inhibitors
Transcriptional machinery is complicated, at first, AR recruits RNA polymerase II and several co-activators, then AR complex binds to the trans-acting factors of its target genes to initiate transcriptional program [34].Downregulation of histone deacetylase 1 or 3 (HDAC1 or HDAC3) may prevent the expression of AR target genes by interfering with the assembly of the AR complex.Unfortunately, a small phase II study about the HDAC inhibitor romidepsin failed to demonstrate sufficient activity in CRPC patients [35].

AR Phosphorylation Inhibitors
AR protein may be phosphorylated at tyrosine and serine/threonine residues.
Etk/BMX tyrosine kinase and Src kinase may phosphorylate AR at Tyr-534, inducing AR nuclear localization, AR recruitment to the promoters, and tumor growth.AR Tyr-223 may be phosphorylated by Fer tyrosine kinase.Ack1 kinase phosphorylates AR at Tyr-267 and Tyr-363, promoting the recruitment of AR to trans-acting regions.Mutant ARs (Y267F and Y363F) inhibit Ack1-mediated AR transactivation and chromatin binding.In addition, cyclin-dependent kinase 1 or 9 (CDK1 or 9)-mediated phosphorylation of AR at Ser-81 associated with the AR-chromatin interaction [36].JNK and p38 kinases phosphorylate AR at Ser-650, regulating the nuclear export of AR [37], whereas dephosphorylation by protein phosphatase 1 regulates the AR transcriptional activity and nuclear localization [38] [39].

Upstream Inhibitors of AR Signaling
The transcription factor GATA-binding protein 2 (GATA2) colocalizes with AR on chromatin to increase the recruitment of co-activators and formation of the AR complex.GATA2 directly promotes the expression of both the wild-type AR and AR-Vs; in turn, the expression of GATA2 is suppressed by AR and its ligands, suggesting a negative feedback regulatory loop [40].
Prostate cancer gene expression marker 1 (PCGEM1) interacts with splicing factors, hnRNP A1 and U2AF65, suggesting a role for PCGEM1 in alternative splicing.Androgen deprivation induces the expression of PCGEM1 and causes its accumulation in nuclear speckles.PCGEM1 regulates the competition between hnRNP A1 and U2AF65 for AR pre-mRNA.While the interaction of PCGEM1 with hnRNP A1 suppresses AR-V3 by exon skipping, its interaction with U2AF65 promotes AR-V3 by exonization [41].

Downstream Inhibitors of AR Signaling
The bromodomain and extra terminal (BET) inhibitor, ABBV-075, inhibited the androgen-stimulated transcription of AR target genes without significant effect on AR protein expression.ABBV-075 displayed a potent anti-proliferative activity in multiple models of resistance to second-generation antiandrogens and inhibited the activity of AR-V7 and AR F877L/L702H.This provides a promising therapeutic option for CRPC patients who have developed resistance to secondgeneration anti-androgens [42].
Transcriptomic profiling analysis of AR mutant (W741L) reveals 3 important genes, including RASD1 (Ras dexamethasone-induced 1), TIPARP (TCDD-inducible poly (ADP-ribose) polymerase), and SGK1 (serum-and glucocorticoid-regulated kinase 1).SGK1 is upregulated in the KUCaPcells and a CRPC patient tissue containing AR mutant resistant to bicalutamide.GSK650394, a SGK1 inhibitor, may reduce the tumor growth induced by AR W741L.These results indicate that SGK1 is a key downstream protein of AR W741L, and SGK1 may be a new and efficient therapy for CRPC patients [43] [44].

Perspective
Previously, when scientists developed a treatment for PCa, it was commonly expected to cure all the PCa patients.However, the accumulation of clinical experiences and advancement in medical knowledge led to the realization that a ma-jority of PCa patients were successfully treated initially; however, recurrence to incurable CRPC with malignant phenotypes occurred after several years.We have to accept the phenomena mentioned above that PCa can evolve to drug-resistant variant cells under the selection pressure.In order to effectively treat PCa, it is essential to completely understand the mechanisms of PCa progression, and to develop novel drugs to counter resistance.This theory potentially explicates the smallest possibility of curing cancer, rendering an endless struggle with the disease.
Nowadays, investigators are attempting to develop new treatments effective against PCa in patients demonstrating resistance to clinical therapies.Additionally, a cocktail of various drugs and key enzyme treatments may increase the curative effect.The inhibitors targeting crucial enzymes or signaling pathway proteins may be more effective because the mutations of key enzymes or proteins induced by drug pressure are mortal to tumor or normal cells.Nevertheless, the side effects of these inhibitors are yet to be verified by several studies.
These may be the developing direction of the PCa treatment in the near future.
Moreover, all these therapies that have been successfully used in the clinic or ongoing trials do not consist of natural ingredients.The treatments cause an extra stimulus to the human body, which might not be an appropriate way to eliminate PCa.Thus, if a therapeutic method cannot bring extra stimulus, simultaneously remove the tumor, or make the body to eliminate the tumor actively, it will be the most yearned treatment.It will be the ultimate goal of developing PCa treatment.
This work was supported by Zhejiang Provincial Science Technology Program of China (2013C33101), Zhejiang medical platform program (2015RCA023).

Table 1 .
AR mutants and drug-resistance.