Neutron Activation Analyses Used to Study Elemental Accumulation in Some Marine Macrophytes ( Mediterranean Sea Coast of Egypt )

The concentrations of Na, Mg, Al, S, Cl, K, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Zn, As, Se Br, Rb, Sr, Zr, Mo, Ag, Sb, I, Cs, Ba, La, Sm, Eu, Tb, Yb, Hf, Ta, W, Au, Th and U in 6 types of marine macrophytes (algae and seagrass): Gelidium pusillum (Stackhouse) Le Jolis, Ulva intestinalis Linnaeus, Amphiroa rigida J.V. Lamouroux, Hypnea sp., Cystoseira sp. and Posidonia oceanica (L.) Delile (seagrass) collected from 3 stations along the Mediterranean Sea coast of Egypt were determined using instrumental neutron activation analysis. The contents of elements in marine macrophytes indicated that they accumulated elements at different levels depending on their type of species (brown, red, green and seagrass) and the ambient water conditions. However, the concentrations of Cr, Co, Ni, Se, Zr, Mo, Ag, Cs, La, Sm, Eu, Yb, Hf, Ta, Au and U were very similar in all samples. In general the levels of classically investigated elements, particularly Mn, Fe, Co, Ni and Zn determined in the macrophytes in the present study are lower or within the wide range of values previously reported for species of these genera sampled along the Egyptian Mediterranean coast.


Introduction
With an estimated population of 132 million inhabitants that increases sharply How to cite this paper: Nassar, N., Kravtsova, A., Frontasyeva, M. and Sherif, M.M. (2017) Neutron Activation Analyses Used to Study Elemental Accumulation in Some Marine Macrophytes (Mediterranean Sea during the summer tourist season, the Mediterranean coast, including its Egyptian part, is particularly subject to strong human pressure [1].The western part of the Nile Delta Coast (Abu Qir Bay), which is polluted by industrial wastes from 22 different factories including food processing and canning, paper industry, fertilizer industry and textile manufacturing, is considered as hot spot area [2] [3].Pollution of the marine environment with trace elements (TEs) remains a topical subject because some of these elements, not frequently monitored up to now (e.g.Sb, Mo, etc.) can be considered as environmental pollutants of ''emerging concern'' [4].The monitoring of marine pollution using different bioindicator species provides several advantages [5] [6] when compared with chemical analysis of environmental matrices (i.e.water and sediments).A wide variety of species have been shown to be relevant indicators to assess the contamination status of marine ecosystems, where different species of seagrass and macroalgae are among the most used in the Mediterranean [4] [7].
During the last decades numerous studies have been carried out concerning trace element accumulation in marine macrophytes sampled along the Mediterranean coast of Egypt.However, data are mostly available for species of a few genera such as Ulva, and only a small number of elements, mainly Cd, Cu, Cr, Mn, Co, Ni, Pb and [2] [3] [8] [9] [10].References concerning widespread macroalgae that could potentially be used as biomonitors and levels of the majority of elements are scarce.To extend the list of potential biomonitors in the study area and add data about accumulation of a wide range of elements, including major and rare earth elements, the aim of this work was to evaluate the concentrations of up to 39 elements in 6 species of macrophytes sampled in the coastal waters of Egypt (Mediterranean Sea) using neutron activation analysis (NAA).

Study Area
The samples were collected during summer 2015 in the coastal waters of the city of Alexandria and Marsa Matrouh city which are both situated on the Mediterranean coast of Egypt and are considered the main two cities and industrial zones on the north coast of Egypt.The sampling sites are presented in Figure 1.
The first sampling site is located at Cleopatra beach, Marsa Matrouh city (31˚22'29.0''N,27˚11'23.0''E).It is characterized by big anthropogenic loading during summer period when the number of visitors to Marsa Matrouh may reach more than one million [10].The second and third sampling sites are adjacent to Alexandria, the second largest city in Egypt and a major economic center, extending about 32 km to the north west of Nile Delta stretching over 70 km along the coast [11].Alexandria's environmental problems have grown in severity as its population and associated urban and industrial development have increased since the beginning of the 20th century [12].The second sampling site is located at Citadel beach (31˚12'49.6''N,29˚53'01.1''E),about 4 km from Alexandria port, which is considered the main port in Egypt.Alexandria Port receives many types of containers (e.g., cement, coal, fertilizers, grain and flour, oil and oil

Sampling and Sample Preparation
The samples that were found attached to rocks were collected at 3 station (1-st -Cystoseira sp. and Posidonia oceanica; 2-nd-Gelidium pusillum; 3-d-Hypnea sp., Ulva intestinalis, Amphiroa rigida) by hand on the depth 0.5 to 1.5 m, rinsed with ambient water and cleaned from epiphytes, then kept in a polyethylene bags and transferred to the laboratory in an ice-box.In the laboratory samples were rinsed with distilled water [7] and dried till constant weight at 40˚C during 24 hours then manually homogenized in agate mortar [15].

Analysis
Neutron activation analysis was performed in the radioanalytical laboratory at the pulsed fast reactor IBR-2 of the Frank Laboratory of Neutron Physics, JINR, Dubna, Russia [16].For short irradiation samples of about 0.3 g were heat-sealed in polyethylene bags.For long irradiation samples of the same weight (about 0.3 g) were packed in aluminium cups.To determine the short lived isotopes (Al, S, Cl, Ca, Ti, V, Mn, Mg, and I) conventional irradiation channel was used.Sam-ples were irradiated for 3 min and measured twice after 2 -3 min and the second one for 20 min after 9 -10 min of decay.Long-lived isotopes were determined using epithermal neutrons in cadmium-screened irradiation channel with neutron flux density Φepi.= 3.6 × 10 12 n/(cm 2 •s).Samples were irradiated for 5 d, repacked and then measured twice after 4 -6 and 20 d of decay, respectively.
Measuring time varied from 1 to 5 h.To process gamma spectra and to calculate concentrations of elements in the samples, software was used that was developed at FLNP JINR [17].The errors in the determined concentrations were in the range of 5% -15% and 30% or greater for those elements (e.g.Zr, Mo, Ag, Au) which concentrations in the samples were at the level of detection.Quality control was ensured by simultaneous analysis of the examined samples and standard reference materials SRM 1632c (trace elements in coal, National Institute of Standard and Technology (NIST)), SRM 1633b (constituent elements in coal fly ash, NIST), 1547 (peach leaves, NIST), 690CC (calcareous soil, Food and Agriculture Organization of the United Nations), 1573a (tomato leaves, NIST), SRM 433 (marine sediments, International Atomic Energy Agency) and BCR 667 (estuarine sediment, Institute for Reference Materials and Measurements) irradiated in the same conditions together with the samples under investigation.The NAA data and certfied values of reference materials are given in Table 1.

Results and Discussion
For all collected samples the concentrations of Na, Mg, Al, S, Cl, K, Ca, Sc, V, Mn, Fe, Co, Ni, Zn, As, Se Br, Rb, Sr, Ag, Sb, I, Cs, Ba, Ta, Th, and U were determined while the content of Ti, Cr, Zr, Mo, La, Sm, Eu, Tb, Yb, Hf, W and Au in some species was below the level of detection (Table 2).
The concentration of major elements varied in a wide range depending on the species of macrophytes (from 1635 mg/kg for K in Posidonia oceanica to 246,500 mg/kg for Ca in Amphiroa rigida).The highest concentration of Na and S were found in Hypnea sp., while the maximum values of Cl and Ca were determined in Ulva intestinalis and Amphiroa rigida, respectively.The concentration of Mg varied in a more narrow range, and their minimum and maximum contents differ in the samples by up to eight times.The results obtained for some trace elements varied in a wide range depending on the species of analyzed macrophyte (from 0.002 mg/kg for Tb in Hypnea sp. to 2100 mg/kg for Sr in Amphiroa rigida while the concentrations of Cr, Co, Ni, Se, Zr, Mo, Ag, Cs, La, Sm, Eu, Yb, Hf, Ta, Au and U were very similar in all samples (Figure 2).
The concentration of elements in the samples collected at the same station (Abo-Qir bay) are varied greatly too.Thus the content of Na, S, Cl, Ca and Sr in Hypnea sp., Ulva intestinalis and Amphiroa rigida differ by more than 10 times, Mg, Ba, Tb and Th differ by up to 10 times.Nevertheless, the levels of such elements as Sc, Ti, V, Cr, Fe, Co, Ni, Zn, Se, Zr, Mo, Ag, Sb, I, Cs, La, Sm, Hf, Ta, Au and U are very similar in Hypnea sp., Ulva intestinalis and Amphiroa rigida and differ by up to 3 times.The accumulation rate of major and trace elements    Our levels of Mn in Ulva intestinalis are 2 -2.5 times higher than the data of other authors, while the content of Fe, Co, Ni and Zn is up to 10 times lower than the concentrations reported for the species of Ulva collected along the Mediterranean coast of Egypt (Table 3).
Gelidium pussilum is characterized by the highest levels of Ni, Br and Rb and the lowest values of Al, Ca and Sc.The contents of Na (21,000 µg/g), Ni, and Zn (Table 3) reported by Shams El Din and El-Sherif (2012) [18] for Gelidium corneum sampled along the western coast of Alexandria are similar to our data, while the concentrations of K (2700 µg/g) and Ca (26,000 µg/g) are an order of magnitude lower and higher, respectively, than our results.
Hypnea sp. is characterized by the highest contents of Na and S and the lowest levels of Cr, Sr, La, Ta, and Tb.Our results obtained for major elements (except for Ca) are 2 -3 times higher than the data reported by El-Said (2013) [9] for Hypnea musciformis collected from Abo-Quir bay, Egypt.The determined concentrations of Fe and As in Hypnea sp. are in agreement with the reported data for species of Mediterranean Hypnea, while in most cases our contents of Co, Ni, and Zn are one order of magnitude lower than the results of other authors (Table 3).
Amphiroa rigida is characterized by the highest levels of Mg, Al, Ca, Ti, Mn, Sr, La and Sm and the lowest values of Na, S, Cl, Ni, As, Br, Mo and Sb.The results obtained for Na and K are in agreement with the data reported by El-Said (2013) [9] for the species of the same family Corallinaceae (Jania rubens ) collected from Abo-Quir bay, while our levels of Mg and Ca are 10 and 100 times higher, respectively, than the data of the same author.The content of Mn, Fe and Zn are similar to the values reported by other authors for Jania rubens sampled along the Mediterranean coast of Egypt.Our levels of Co and Ni are up to one order of magnitude lower than the literature data (Table 3).
The maximum values of K, V, Zn, As, Mo and Ba and the lowest contents of Mn and Fe are found in Cystoseira sp.our concentrations of Na and Ca are in agreement with the data reported by Shams El Din and El-Sherif (2012) [18] for Cystoseira spinosa sampled along the western coast of Alexandria, Egypt, while our value for K is 23 times higher.Also our levels of major elements (Na, Mg, Cl, K, Ca, Sr) and halogens (Br, I) are similar to the published results for Cystoseira sp.collected along the Syrian coast of the Mediterranean Sea [7] differing only by a factor of 1.5 -2.0 Level of Fe detected in this survey is in good agreement with the data reported for the species of Cystoseira sampled along the Mediterranean coast of Egypt, while our content of Ni is about 5 times lower.
Posidonia oceanica is characterized by the highest level of Sc, Cr, Se, Sb, I, Hf, Th and U and the lowest values of K, Ti, V, Zn and Rb.Our concentration of Na, K and Ca are 2 -3 times lower than the data reported by Shams El Din and El-Sherif (2012) [18] for Posidonia oceanica collected along the western Egyptian Mediterranean coast.The levels of Fe and Ni determined in this survey are in agreement with the results of Khaled (2014) [10] published for Posidonia oceanica collected near Marsa Matruh, Egypt, Mediterranean Sea.
In general the levels of Mn, Fe, Co, Ni and Zn observed in Ulva intestinalis, Gelidium pussilum, Amphiroa rigida, Hypnea sp., Cystoseira sp. and Posidonia oceanica in the present study are within the wide range of those previously re- ported for species of these genera sampled along the Egyptian Mediterranean coast (see Table 3).References concerning other trace and major elements in studied seaweeds are too scarce to establish comparisons.
The present results indicate that the accumulation of several elements in seaweeds is largely related to the phylogenetic origin of the species, which determines the biochemical composition of macroalgae.Thus, brown algae (in our study-Cystoseira sp.) usually accumulate more As, Sr and U than other taxonomic groups.High levels of as in brown algae are due to high phosphate concentrations in these macroalgae [21], as seaweeds take up and bioaccumulate arsenate from seawater as a phosphorus analogue [22].High Sr concentrations in brown macroalgae are related to the cell wall polysaccharide alginate (constitute about 10% -40% of brown algae dry weight), as the main accumulation mechanism for Sr in brown algae is an ion exchange between seawater and alginate [20].High concentration of Ca (about 25% of dry weight) in Amphiroa rigida could be explained by calcareous deposits contained within the cell walls which are typical for all algae of the order Corallinales.Elevated levels of Sr in Amphiroa rigida when compared to other studied macrophytes are related to high con- tent of Ca which geochemical and biochemical characteristics are similar to those of Sr [23].
However, high concentrations of some trace elements in macrophytes may also be explained by their elevated level in the water area of sampling.Thus the maximum contents of V, Zn, Mo in Cystoseira sp. and Cr and Ni in Gelidium pussilum are likely due to relatively high level of pollution of sampling sites.

Conclusion
The concentrations of more than 30 elements that were rarely or never studied as well as the levels of classically investigated Mn, Fe, Co, Ni and Zn in 6 species of marine macrophytes sampled along the Egyptian coast of the Mediterranean Sea were determined using neutron activation analysis.The results obtained for major and some trace elements varied within a wide range depending on the species of analyzed macrophyte, while the concentrations of Cr, Co, Ni, Se, Zr, Mo, Ag, Cs, La, Sm, Eu, Yb, Hf, Ta, Au and U were very similar in all samples.
The contents of Na, S, Cl, Ca and Sr in Hypnea sp., Ulva intestinalis and Amphiroa rigida sampled at the same station (Abo-Qir bay) differ by more than 10 times; Mg, Ba, Tb and Th differ by up to 10 times indicating that the accumulation of these elements is closely related to species biochemical composition, thallus morphology, or growth strategy.Our results indicate that Cystoseira sp. is a strong accumulator of As, Sr and U, and Amphiroa rigida of Ca and Sr.The studied species of macrophytes might be regarded as potential biomonitors for the elements concerned along the Egyptian coast of the Mediterranean Sea.
Nevertheless, future investigations should be conducted in water areas with different levels of anthropogenic pollution, including relatively pristine areas, to reveal other accumulation properties of the macrophytes studied.

Figure 1 .
Figure 1.Sampling locations on the Mediterranean Sea in the north coast of Egypt.
by marine macrophytes depends on many factors, where the taxonomic identity of the plant and concentration of elements in the ambient environment are among the most important ones.The collected marine macrophytes represent three algal classes and seagrass species: Chlorophyceae (Ulva intestinalis), Rhodophyceae (Gelidium pussilum, Amphiroa rigida, Hypnea sp.), Phaeophyceae (Cystoseira sp.) and Tracheophyta (Posidonia oceanica).The maximum values of Cl and Fe and the lowest content of I are found in Ulva intestinalis.Our levels of Na, K and Ca are 2 -3 higher than the data reported by El-Said (2013) for Ulva lactuca collected from Abo-Qir bay, Egypt.

Figure 2 .
Figure 2. Concentration of trace elements in mg/kg dry weight in marine algae and seagrass collected from the Mediterranean coast of Egypt.

Table 1 .
Analysis of certified reference materials: certified and determined values, standard deviation.

Table 2 .
Concentrations of elements (mean ± standard error, in µg•g −1 dry weight) in marine macrophytes (the Mediterranean coast of Egypt).

Table 3 .
Concentration of some trace elements (in µg × g −1 dry weight) in marine macrophytes (the Mediterranean Sea).