Yield Compounds and Nutrient Elements of Carnation ( Dianthus caryophyllus L . ) under Different Growing Media

Production of cut flowers is very prevalent in Iran. Carnation is most famous for its use as a cut flower. This study was carried out in the research greenhouse of Islamic Azad University, Isfahan (Khorasgan) Branch, to explore the effect of different growing media on some vegetative and reproductive traits of carnation. The experimental design was randomized complete, using 50% perlite and different growing media including: mineral soil and coco peat in 6 proportions (50% coco peat, 10% soil + 40% coco peat, 20% soil + 30% coco peat, 30% soil + 20% coco peat, 40% soil + 10% coco peat, 50% soil). During growth period, irrigation rate, humidity and temperature were similar. The growth characteristics of carnation were determined at the end of growth period. The results indicated that although there were no significant differences between studied growing substrates in chlorophyll a, b. Statistical analysis showed fresh weight of flower was far greater in coco peat 10% + soil 40% and soil 50% compared to other growing media. Fresh and dry weight of root and shoot in soil 50% were higher than other growing media. The results showed that highest concentration of Fe and Zn was observed in coco peat 40% + soil 10%. The concentration of Mn in coco peat 10% + soil 40% was higher than other growing media. The results showed that the highest Ca concentration was observed in soil 50% and the lowest concentration of this element was obtained in coco peat 50%. According to the obtained results, mineral soil can be used as a suitable alternative to cocopeat for cultivation and growth of carnation in greenhouse.


Introduction
Carnation (Dianthus caryophyllus L.) has high ornamental value and easy technology for production of cut flower.It is the main floriculture crop in many countries.Carnation is most famous for its use as a cut flower.The carnation flower is a wonderful accent to bouquets and carnation home floral arrangements.An essential oil is obtained from carnation flowers, which is applied in perfumery where 500 Kg of flower produce 100 gm of oil.Also, the flower heads are dried and used in sachats and cosmetic.Carnation flowers are considered to be antispasmodic, cardiotonic, alexiteric, nervine, and diaphoretic [1].According to previous studies, suitable growing media are necessary for gas exchange between plant roots and atmosphere, supply of nutrients and water quality flower production as well as sufficient anchorage of plant [2].Porosity of growing media is an index for root media aeration.When root media aeration is suitable, supplement of nutrient elements and water for growth of plant is easily [3].Nutrient availability is one of the important factors influencing the suitability of organic substrates for plant growth and development [4].However, different growing substrates have several materials which could have effects on plant production.Thus, selecting the best substrate among the various materials is imperative to the plant productivity [5].
In previous studies such as Omidbaigi and Arjmandi [6] and Baranauskiene et al. [7], the application of chemical fertilizers for improve in crop yield is well confirmed.Also, few works showed that organic fertilizers resulted in increases in dry plant yield [8] [9].Samiei et al. [10] examined the impacts of date-palm and peat moss wastes on growth traits of Aglaonema.They found both growing substrates were similar in some plant properties.Agriculture production needs sustainable and environmentally fertilizer management practices.Soil fertility is the most substantial and controlled factor affecting the nutritional content of plant.The production of organic food has been demonstrated to inhibit chemical fertilizers, herbicides, and pesticides and centralize on natural material and methods of controlling the plant growth, crop yield and plant diseases [11].The organic fertilizers may serve as alternative practice to chemical fertilizers [12] for improving soil microbial activity [13] and soil structure [14].With attention to rapid development of greenhouse cultivations for production of cut flowers such as carnation, the selection of a suitable substrate for cultivation and growth of carnation is required.Therefore, the aim of this study was to investigate the influence of these culture media on some vegetative and reproductive traits of carnation.

Materials and Methods
A greenhouse experiment was conducted at the research greenhouse of Isfahan (Khorasgan) branch during 8 months at 2013-2014 to explore the effect of different growing media on some vegetative and reproductive traits of carnation (Nuska).The experimental design was a completely randomized design replicated five times.The treatments were 50% perlite and different growing media including: mineral soil and coco peat in 6 proportions (50% coco peat, 10% soil + 40% coco peat, 20% soil + 30% coco peat, 30% soil + 20% coco peat, 40% soil + 10% coco peat, 50% soil).Plant saplings were planted in pots (4 L) filled with the above growing substrate.During growth period, humidity was similar.Average temperature of day and night were 25˚C ± 2˚C and 17˚C ± 2˚C, respectively during growth period.The pots were irrigated three and two times in the spring-summer and autumn-winter seasons, respectively.Salt leeching by water was used to inhibition of salt accumulation.The concentration of nutrient elements in modified Hoagland for cultivation of carnation is listed in Table 1.

Soil and Coco Peat Analysis
The electrical conductivity (EC) in saturated extracts and soil pH was determined in the soil saturation paste.However, pH and EC of coco peat were measured in water extracts (1:5 v/v).In soil and coco peat, total nitrogen (the Kejldahl method), available K (with ammonium acetate) available P (the Olsen's method) and cation exchange capacity (CEC) with NH4OAc method were determined via procedures described in Baruah and [15].Also, the physical characteristics of soil and coco peat including water holding capacity [16], bulk density [17] and porosity [15], were measured before planting.Soil texture was performed using the hydrometer method [18].Some chemical and physical properties of mineral soil and coco peat are listed in Table 2.

Plant Analysis
Some growth indices including fresh and dry weight of flower, shoot and root were determined at the end of growth period.Chlorophyll (Chl) was estimated by extracting the leaf material in 80% acetone.Absorbance was recorded at 663 and 645 nm.Chl (a) and Chl (b) were calculated as described by Arnon [19].The contents of nutrient elements in plant were determined by drying samples at 70˚C and then ashed at 500˚C under a gradual increase in temperature.Then, 10 mL hydrochloric acid 6 M (1 + 1) was added and the solution was evaporated in water bath to dryness.The residue was dissolved in 50 mL distill water.The concentrations of P and K were measured by colorimetrically and flame photometry methods, respectively.Also, the concentrations of Ca, Mg Fe, Mn and Zn were measured either by atomic absorption spectrophotometry (Perkin Elmer Analyst 800).

Statistical Analysis
One-way ANOVA was used to assess the effects of different growing media on all analyzed plant properties.Means were compared by Duncan test (p < 0.05).Statistical procedures were carried out using the software package SAS 9.1 for Windows.

Flower Weight
As shown at Table 3, the analysis of variance revealed that the effect of substrate on fresh and dry weight of flower was significant (p < 0.01).Statistical analysis showed fresh weight of flower was far greater in coco peat 10% + soil 40% and soil 50% compared to other growing media (Table 3).However, dry weight of flower was far higher in soil 50% compared to other growing media.Reference to fresh and dry weight of flower, the differences between coco peat 50% with coco peat 20% + soil 30%, coco peat 30% + soil 20% and coco peat 40% + soil 10% were not statistically different at 5% probability level, according to the Duncan test (Table 3).

Root Weight
There were significant (p < 0.01) differences between culture media in fresh and dry weight of root.These properties in soil 50% were higher than other growing media, however the differences between other growing media were not statistically different at 5% probability level, according to the Duncan test (Table 3).

Shoot Weight
As shown at Table 3, the analysis of variance showed that the effect of substrate on fresh and dry weight of shoot was significant (p < 0.01).Statistical analysis showed fresh weight of shoot was far greater in soil 50% compared to other growing media (Table 3).Also, lowest fresh weight of shoot was obtained in coco peat 50% and coco peat 40% + soil 10%.The results showed that highest dry weight of shoot was observed in soil 50% compared to other growing media.Also, lowest dry weight of shoot was obtained in coco peat 50%, although the difference between coco peat 50%, with coco peat 10% + soil 40% was statistically different at 5% probability level, according to the Duncan test (Table 3).

Nutrient Elements in Plant
Table 4 presents the effect of different growing media on nutrient elements in plant.There was no significant effect of treatment on concentration of P and ranged from 0.066% to 0.072%.As shown at Table 4, the analysis of variance showed that the effect of substrate on concentration of K was significant (p < 0.01).The concentration of K in coco peat 40% + soil 10% and soil 50% were lower than other growing media, although in some cases, the differences were not significant (Table 4).There were significant (p < 0.01) differences between culture media in Ca concentration.The results showed that highest Ca concentration was observed in soil 50% and lowest concentration of this element was obtained in coco peat 50%.As shown at Table 4, the analysis of variance showed that the effect of substrate on concentration of Fe and Zn was significant (p < 0.01).The results showed that highest concentration of Fe and Zn was observed in coco peat 40% + soil 10% and lowest concentration of theses element was obtained in coco peat 20% + soil 30%.There were significant (p < 0.01) differences between culture media in concentration of Mn.The concentration of Mn in coco peat 10% + soil 40% was higher than other growing media, however, lowest concentration of Mn was obtained in coco peat 50% and coco peat 20% + soil 30% (Table 4).Note: S.O.V: source of variation, df: degrees of freedom, C.V: coefficient of variation, ** p < 0.01.In each column, the values followed by at least one common character are not statistically different at 5% probability level, according to the Duncan test.

Plant Chlorophyll Content
Table 5 presents the effect of different growing media on plant chlorophyll (chlorophyll a, b and total chlorophyll) content.There was no significant effect of treatment on content of chlorophyll a, chlorophyll b and total chlorophyll.The content of chlorophyll a ranged from 0.38 to 0.45 mg/g and for chlorophyll b ranged from 0.42 to 0.48 mg/g.However, the content of total chlorophyll in coco peat 40% + soil 10% and soil 50% were higher than other growing media, although in some cases, the differences were not significant (Table 4).

Discussion
The type of growing media used in this study performed an important role in plant characteristics of carnation.
The results showed that in most cases, the plant growth properties were significantly higher in mineral soil than other growing media.The increase in the characteristics of carnation with application of mineral soil can be explained on the basis of increases in concentration of nutrient elements.These results are concurred with the findings of Olympios [5] and Kumar and Goh [20] who discovered that physiochemical properties of growing media affected on plant growth and yield.The nutrient elements in the solution of different growing media are unique due to the different type and origin of media.Rostami et al. [21] reported that the highest yield and growth of strawberry possessed by date palm growing media.According to findings of Safaei et al. [22] the combination of NPK and manure fertilizers could be suggested as an appropriate nutrition source for thyme and the improvement of soil structure.However, perlite substrate with very low cation exchange capacity (CEC), and good capacity of water absorption and coco peat substrate, with its high water holding capacity and nutrients can be considered as good growing media in soilless culture [23].Nowak and Strojny [24] confirmed that the total porosity, bulk density, and air capacity of the growing media had significant effects on growth of plant.Mohamadi-Ghehsareh et al. [3] found that fruit yield, shoot height and root weight of cucumber related to date-palm waste had a significant difference as compared with soil, however, this growing media had not significant difference as compared with perlite.The water holding capacity and aeration have likely an important role on plant growth and development [25].The results showed that highest concentration of Fe and Zn was observed in coco peat 40% + soil 10%.The concentration of Mn in coco peat 10% + soil 40% was higher than other growing media.The results showed that highest Ca concentration was observed in soil 50% and lowest concentration of this element was obtained in coco peat 50%.Kalantari et al. [26] reported that vermicompost increased the concentrations of P, K, Ca, and Mg in the shoot of corn.However, Stamatiadis et al. [27] stated that the concentrations N, P, K, Ca, Mg, Zn, Fe in broccoli leaves were not significant in control, compost and ammonium nitrate treatments.
There was no significant effect of treatment on content of chlorophyll a, chlorophyll b and total chlorophyll.The content of plant chlorophyll reflects the photosynthetic assimilation ability and photosynthetic rate [28].In contrast with findings obtained in present study, Doan et al. [29] found that chlorophyll content maize leaves was greater in control compared to the compost and vermicompost treatments.Also, Fernández-Luqueño et al. [30] illustrated that the chlorophyll content of the beans in the vermicompost was higher than sludge and urea.

Conclusion
The performance and suitability of different medium for the carnation (Dianthus caryophyllus L.) were studied by employing six different substrates in a greenhouse.It is evident from the results that application of mineral soil (the combination with perlite) as growing media can support better carnation growth, when compared to the coco peat.

Table 2 .
Some chemical and physical properties of mineral soil and coco peat.

Table 3 .
ANOVA model and comparison of flower, root and shoot weight (g) for different growing media.
Note: S.O.V: source of variation, df: degrees of freedom, C.V: coefficient of variation, ** p < 0.01.In each column, the values followed by at least one common character are not statistically different at 5% probability level, according to the Duncan test.

Table 4 .
ANOVA model and comparison of some nutrient elements in plant for different growing media.

Table 5 .
ANOVA model and comparison of some plant traits for different growing media.* p < 0.01.In each column, the values followed by at least one common character are not statistically different at 5% probability level, according to the Duncan test.