The Effects of Aerobic Exercise Training on Basal Metabolism and Physical Fitness in Sedentary Women

Objective: Aerobic exercises are the basic activity on fight against obesity. And obesity is related with metabolic rate. So our study is aimed to investigate the effects of 8 weeks aerobic exercise on basal metabolic rate and physical parameters. Methods: Sedentary women between the ages of 35 45 (n = 40) were randomized into control group (CG) (n = 20) and exercise group (EG) (n = 20). EG underwent 8 weeks of aerobic-run-walk exercise training: 3 days a week, 1 hour sessions. The CG was not trained. Basal metabolic rate (BMR), body mass index (BMI), waist-hip ratio (WHR), body fat percentage (BFP), body fat mass (FM) and lean body mass (LBM) were measured for all of the subjects before and after the training program. Results: Mean BMR decreased from 1386 ± 213.6 kcal to 1327 ± 253.7 in CG, and raised from 1308 ± 201.8 to 1409 ± 218.3 kcal in EG. While BMI raised from 31.39 ± 6.15 kg/m2 to 31.51 ± 6.09 kg/m2 in CG, it decreased from 29.62 ± 3.78 kg/m2 to 28.47 ± 3.74 kg/m2 in EG. There was also statistically significant difference in parameters of WHR, BFP, FM and LBM in favour of EG (p < 0.05). Conclusion: After 8 weeks aerobic exercise training program, there was a statistically significant difference in favour of EG in BMR, BMI, BFP, FM, LBM, WHR and weight parameters.


Introduction
The importance of exercise in daily life has increased due to developing technology and studies focusing on this subject.Obesity which is closely associated with energy metabolism is a very common health problem in nearly all societies and becoming a global epidemic [1].Obesity is common in the United States and is a major public health problem because of its association with considerable morbidity, including hypertension, diabetes mellitus, and coronary artery disease [2] [3].Sedentary lifestyle is a major factor causing obesity.The appearance of increase in the risk of hypertension, metabolic syndrome and diabetes in adults if sedentary lifestyle becomes more widespread in the future, emphasizes the need of focus to health and coronary heart disease on society in terms of protection [4].Lately though, as a result of the emphasis on this requirement, a partly awareness of sport has begun to occur.Due to this awakening, new sports options have been consisting.For example, the number of people who have been deailng with Pilates in the United States exceeded 5 million at this point [5].Exercises which everyone will find easier and to do by one's own such as cycling, walking and running, maintain their own importance and popularity from the past to the present.Latey (2001) pointed out that this increase in people's demand on doing sports occured due to protection from injuries and wellness status [6].Fundamental elements of being healthy are balanced body composition, physical fitness and to be able to keep it well [7].Various techniques like BMI, skinfold, circumference measurements and bioelectrical impedance analysis (BIA) are used to determine physical fitness [8].
Metabolism and its speed are also effective factors on physical well-being.Anabolic and catabolic processes in the organism are always in equilibrium.Almost all the body's cells are renewed permanently, they are synthesized apart again.Also, metabolic activity can be described as generating new molecules from one to another [8].Return of surplus proteins and carbonhydrates to fat in the organism is basically related with this metabolism [9] [10].BMR, which is an important parameter of energy metabolism, is the lowest amount of energy, necessary for the vital functions of the person [11].Basal metabolism comprises 60% to 75% of the daily energy expenditure [12].Activities of the brain, heart, kidneys and other organs constitute a significant portion of BMR, the main cause of differences between people are amount of skeletal muscles, the fat mass and body size [13].Therefore metabolic rate is lower in women compared with men [2].In people whose LBM is more, their BMR is higher too.Between the ages of 20 -40, for each m 2 of body surface area men spend 38 kcal, while women spend 35 kcal average [14] [15].Metabolic activity of adipose tissue, and therefore energy consumption is lower than in the muscle tissue.The changes of body combine which occur in adulthood, so agerelated increase in fat mass and decrease in lean mass explains the decrement of 2% -3% of BMR in adult men and women decennially [12].
The effects of exercise on basal metabolism is the subject of researches for many years.Studies obtained different results are available on this topic [16]- [19].There is no consensus regarding the long-term effects of regularly done physical activity on basal metabolism.This disagreement raises the necessity of new researches.Further studies are needed to define the role of energy metabolism on determining exercise approaches to treat obesity.Therefore the aim of this study is to examine the effect of 8-week aerobic exercise on basal metabolic rate and physical parameters in premenopausal sedentary women.Individuals with a history of chronic disease, surgery, smoking, having or had on a diet or exercise program for last one year, or in a pregnancy or breastfeeding period were excluded.Individuals were selected by criteria on to be sedentary women in premenopausal period and between the ages of 35 -45.

Materials and Methods
Before starting the research participants were informed about the content of the study, its purpose, application and the potential risks.Voluntary consent forms were distributed and were signed to all participants.Consent was obtained from "Malatya Clinical Research Ethics Committee" for this research (decision no: 2011-32).Subsequently, CG had not undergone exercise program; EG did aerobic-run-walk exercise 1 hour a day, 3 days a week for 8 weeks.BMR, BMI, WHR, BFP, FM and LBM were measured for all of the subjects before and after the training program.

Study Design
At the weeks before the beginning and immediately after the 8 weeks of aerobic exercise, volunteers were invited to the laboratory for measurements.BMR measurement which was to be done on resting condition and after 12 hours of fasting, performed between 8:00 to 10:00 am.According to the admission criteria, from the day of measurements subjects were required to avoid from smoking environment.
Temperature of room which measurements will be performed in, fixed with air conditioning to 22˚C.Also necessary isolation provided for not to change of humidity, light and sound.Light dose was isolated to be slightly dark and quiet room.All measurements were performed by an experienced person using the same equipment for each subject.
The measurements were made in the following order: • Height and weight values required for the BMR measurement was measured and recorded.
• For BMR measurement each participant was allowed to rest on supine position 30 minutes (min.)prior of measurement.
• BMR measurements performed for 30 min.after resting period.
• BMR measurement ending subjects were taken into BIA measurements.
• Subsequently circumference measurements were performed.

Height and Weight Measurement
Height measurement is powered by a Soehnle brand gauge working with ultrasound method [20].
Tanita brand bioelectrical impedance analyzer "Tanita Body Composition Analyser BC-418" was used for weight measurement.Weight of subject's clothes were allowed to be deducted from the weight of subject.After all reading from the device's LCD screen values have been saved [20].

BMR Measurement
Fit Mate ® BMR measurement device of Cosmed firm was used.The subjects was measured after 30 min. of rest in the supine position.Sensor mask of device was used separately for each participant.Subjects were measured while normal breathing for 30 min.Subjects warned about not to move during the measurement.At the end of measurement automatic output was received and the BMR value (kcal) recorded [21].

Bioelectrical Impedance Analysis (BIA)
"Tanita Body Composition Analyzer BC-418" was used for BIA analysis.Height (cm), age (years) and subject's clothes weigt (kg) values were entered to divice.With this device LBM, BFP, FM parameters were calculated and automatic output received.BMI value was calculated using the formula weight (kg)/height (m) 2 [22].

Circumference Measurements
Circumference measurements were performed using standard tape measure (Gullick strip).For determining of waist-to-hip ratio, 2 region of body measured (waist, hips).The measurement region of subjects was allowed to be naked.Circumference measurements were repeated three times to each point and taking the mean value of these three measurements was recorded.
Waist: waist measured at the narrowest area parallel to the ground during normal breathing.Hips: maximum protrusion of the buttocks muscle (gluteus maximus) were measured in parallel position over the place [8].WHR value obtained by dividing waist circumference to hip circumference (WHR = waist circumference/hip circumference) [23].

8 Weeks Exercise Protocol
Run-walk exercises are made in the 1700-meters jogging track located on the Inonu University campus.Heart rate was controlled with portable polar device during exercise.Exercise intensity was determined with Karvonen Method [24] and the number of heart beats were calculated for each subject separately [11].Exercise group did run-walk exercises in the intensity of 60% target heart rate 1 hour a day, 3 days a week for 8 weeks.Cool-Down 5

-8
Same with firts week 60 6 3 Warm-up Period (10 min.):• Light tempo 5 min.jog, direction-changing walks, • Warm-up and stretching exercises for the muscle groups to be used in the exercise [25].

Statistical Analysis
Mann-Whitney U test was used to determine whether there are differences in age, height and weight parameters between exercise and control groups in this study.Normal distribution of data was examined by the Kolmogorov-Smirnov test.Two-way analysis of variance (ANOVA) was used for comparison of pre-test -post test results.All statistical analyzes were performed using SPSS program and the significance level was set at 0.05.

Results
After randomisation of 40 healthy female volunteers into two groups; control group (n = 20) and exercise group (n = 20) Mann-Whitney U test was used for comparison of age, height, weight, WHR, BMI, BFP, FM, LBM and BMR between two groups before the intervention.There was no statistically significant difference between CG and EG (p > 0.05).As shown in Table 2 waist circumference values remained unchanged after the final test in the control group, a statistically significant reduction is detected in the exercise group (p < 0.05).While hip circumference increased in the control group, decrease was observed in the exercise group and the difference was statistically significant (p < 0.05).Waist-hip ratio remained unchanged in the control group, decreased in the exercise group and according to the latest test scores this difference was found statistically significant (p < 0.05).While BMI raised from 31.39 ± 6.15 kg/m 2 to 31.51 ± 6:09 kg/m 2 (0.38%) in CG, it was decreased from 29.62 ± 3.78 kg/m 2 to 28.47 ± 3.74 kg/m 2 (3.11%) in EG.While LBM was raised from 46.63 ± 4.68 kg to 46.69 ± 5.03 in CG, it was increased from 50.62 ± 5.41 kg to 51.55 ± 5.95 kg in the exercise group.BFP was decreased 3.02% in the control group, 6.76% in the exercise group.FM was decreased 4.84% in the control group, 9.42% in the exercise group and there was a statistically significant difference between groups according to the last test results (p < 0.05).BMR value remained unchanged in the control group, increased in the exercise group and according to the test scores this difference was found statistically significant (p < 0.05).Figure 1 presents the change of BMR value in CG and EG.

Discussion
There are several studies about the aerobic exercises and their effects.Our study has been focused on aerobic exercise too and carried out to investigate the effects of 8 weeks of aerobic-run-walk exercise training on BMR and physical fitness parameters in sedentary women between the ages of 35 -45.After 8 weeks aerobic run-walk exercise program there was a statistically significant difference between CG and EG in the parameters of BMR, BMI, BFP, FM, LBM, WHR and weight (p < 0.05) (Table 3).In this respect, Amano et al. have reported that obese men and women who underwent aerobic exercise training for 30 minutes, three times a week, over 12 weeks had significant reduction in body weight, BMI, BFP, FM and LBM in their study [1].Again like that as, William et al. were randomly assigned sedentary women to one of three groups that either 1) performed 25 min.step aerobic exercise only; 2) performed a combination of 25 min. of step aerobic exercise and a multiple-set upper and lower body resistance exercise program; 3) performed 40 min of step aerobic exercise only to investigate the effects of different exercise groups to physical and physiologic performance parameters and observed significant reduction in BFP (5% -6%) in all training groups after training [26].
In a randomized controlled trial, 60 patients were randomly divided into 3 groups: control group, group with aerobic exercise, and group with resistance exercise in a study.After 12 weeks of exercise, statistically significant decreases in BMI and FM were noted in groups with aerobic and resistance exercises [27].Struggling with obesity gained further importance nowadays and thus easily accessible and inexpensive methods like exercise and results of the studies about the exercise gained importance [17] [27]- [29].
Our study showed that positive effects can be achieved with only an 8-week aerobic exercise program.The results of the study are valuable in the fight against obesity.The role of aerobic exercise in increasing basal metabolism is trying to be identified with studies.At this point, the results obtained from our study appears to provide a significant contribution.
In a randomized controlled intervention trial, Geliebter et al. assigned sixty-five (25 men and 40 women) moderately obese subjects (aged 19 -48 y) to one of three groups: diet plus strength training, diet plus aerobic training, or diet only.Statistically significant differences were found in BMR in all of the groups [30].Douglas and Poehlman investigated 82 young women who separated into three groups: sedentary (n = 48), aerobically trained (n = 21), and resistance trained (n = 13); only aerobic exercise group had statistically significant difference in BMR than the other two groups [31].These studies show aerobic exercises are more effective on the basal metabolism when compared to resistance exercise but our study provides answers to the questions of how often, with how much load and how long these exercises should be done.In our study, BMR was found to decrease from 1386 ± 213.6 kcal to 1327 ± 253.7 in CG, raised from 1308 ± 201.8 to 1409 ± 218.3 kcal in EG and there was a statistically significant difference between EG and CG (p < 0.05) (Table 4).After 8 weeks of aerobic-run-walk exercise training: 3 days a week, 1 hour sessions there was a statistically significant BMR difference in our study.
Studies concerning how metabolic activity is influenced by exercise in obese, gain more importance day by day.In our study, 40 subjects with the mean BMI 31.45 and defined as obese according to obesity index by the WHO were evaluated and effects of the exercise on obesity parameters were investigated [22].This study's limitation was to be worked with a small number of objects.So there is need of new studies including large number of subjects to define the role of aerobic exercise on basal metabolism and physical fitness.

Conclusion
BMR value increased significantly in the exercise group and patients with "obesity" status got in the "overweight" category.Consequently, 8 weeks of aerobic exercise is effective in increasing basal metabolic rate and normalising physical fitness in sedentary women due to our study results.

Note
This study was presented as a poster presentation at the International Gender and Sports Symposium, Ankara-2014.

Figure 1 .
Figure 1.Change of BMR in EG and CG.

Table 1
shows the training program.

Table 1 .
Applied training program.

Table 2 .
Descriptive statistics of circumference measurements.

Table 3 .
Change of the physical fitness parameters.

Table 4 .
Change of BMR values.
* There was a statistically significant difference (p < 0.05).