Structural Factors of Annonaceous Acetogenins and Their Semisynthetic Analogues Related with the Toxicity on Spodoptera frugiperda

Toxic and nutritional effects of annonaceous acetogenins and their semisynthetic analogues on Spodoptera frugiperda were evaluated. Structural modification of the natural ACG, blocking the OH flanking THF with MOM, allowed us to suggest the mode of action of ACG in the membrane. Our study emphasizes the role of the flanking OH and acetyl groups of THF with the membrane hydrophilic polar head groups. They are essential structural factors in the ACG that facilitate the intermolecular interaction that dehydrates the membrane and makes it potentially toxic against Spodoptera frugiperda.


Introduction
Annonaceous acetogenins (ACG) are a large group of natural products exclusively isolated from the Annona-ceae family.They are characterized by a common skeleton usually formed by 35 to 37 carbon atoms, which have a long alkyl chain terminal end that usually exhibits a γ-methyl-γ-lactone, α, β-unsaturated.The hydrocarbon chain attached to position 2 lactone may have one, two, or more rarely, three-ring tetrahydrofuran (THF) and oxygenated functions and double bonds.
Synthetic analogues, such as the THF central rings of ACG and several structural modifications have been the subject of various studies in the literature [1] [2].
The most important effects of ACG have been described on cancer cell lines, especially those resistant to chemotherapy.Their cytotoxicity is due to the fact that they inhibit ATP synthesis at the mitochondrial complex I. Apparently, inhibition of the respiratory chain would be the most important mechanism through which ACG cause insect larval and pupal mortality.On other hand, Di Toto Blessing et al. (2012) suggested that mitochondrial Complex I inhibition was not the only mode of action of annonaceous acetogenins, since insecticidal action would be by the destabilization that occurred in the membrane due to dehydration around the phosphate groups caused by interaction with ACG and their synthetic analogues [3] [4].
In addition, the insecticidal properties of acetogenins against several key crop pests in different parts of the world have repeatedly been described [5].Spodoptera frugiperda (J.E.Smith) is a polyphagous lepidopteran commonly called fall armyworm, a major pest in corn fields where it feeds on leaves, tassels and ears of corn [6]- [8].It has become one of the most serious problems for corn crops in tropical and subtropical regions of Latin America for the important damages it produces.S. frugiperda displays a very wide host range with over 80 plants recorded, though grasses are preferred.Severe damages are particularly caused during its last two larval stages [9]- [12].
Previous results from our laboratory indicated that the natural ACG had larvicidal and pupal mortality effects on the corn pest, S. frugiperda (Lepidoptera: Noctuidae) [13]- [17].Additionaly, treatment with R. occidentalis acetogenin fraction is environmentally selective and shows an excellent degree of selectivity towards beneficial insects minimizing the detrimental effects of pesticides on natural enemies, allowing their survival and sustainable control of pests [18].Especially the seeds are a promising source of ACG that can be used as a prototype model and/or a biorational insecticide for the control of S. frugiperda.Furthermore, toxic effects of ACG have been reported for several species of insects, S. littoralis, Leptinotarsa decemlineata, Mizus persicae [5].
The aim of this work was to find a relationship of the insecticidal properties on S. frugiperda between natural ACG and their semisynthetic analogues.We first carried out the isolation of the mono and bis-THF ACG from Annona montana and A. cherimolia, and prepared their acetylated (OAc) and methoxy methylated (MOM) derivatives by chemical methods.ACG analogues were synthesized and characterized by spectroscopic techniques (IR, 1 H-NMR, 13 C-NMR, and MS) [1].
We evaluated the toxic effects produced by ACG and their semisynthetic analogues on S. frugiperda.Additionally, Consumption Index (CI), Growth (GR), and Efficiency in the Consumption Index (ECI) were also assessed.

Experimental Analysis
Plant material: Annona cherimolia seeds from "chirimoya" fruits were collected in Tucumán, Argentina.A. montana seeds from "sinini" fruits were collected in Santa Cruz de la Sierra, Bolivia.
Structurally modified ACG: Acetylated analogues, annonacin (3OAc) (2), annonacin (4OAc) (3), rolliniastatin-2 (3OAc) (5), and itrabin (3OAc) (7), were obtained by chemical acetylation with acetic anhydride at room temperature under a nitrogen atmosphere and with the addition of triethylamine.The residue obtained after solvent evaporation was chromatographed on silica gel.The compounds of interest were purified by column chromatography and identified by spectroscopic methods of high resolution NMR and MS.
Methoxy methylated derivative, itrabin (3MOM) (8) was obtained by adding N,N-diisopropylethylamine and methoxymethyl chloride to a solution of ACG 6 in dichloromethane under a nitrogen atmosphere.The reaction was followed by TLC until the formation of products.The residue obtained after solvent evaporation was chromatographed by flash column.The identification of the bis-THF ACG methoxy methylated 8, was achieved by comparison of spectroscopic data 1 H-NMR and 13 C-NMR with the sample of the original ACG 6.

Treatment Formulations
Test solution: Natural ACG and their semisynthetic analogues solutions were prepared at 100 μg/mL, all under the same conditions [7].
Toxicity test: The assays were recorded for treatments with all acetogenins (100 µg/mL) and control experiments.The nutritional indices were determinated by the Consumption Index (CI), Growth Rate (GR), and Efficiency in the Consumption Index (ECI).For comparison purposes, rates are expressed as a relationship between treatment and control; the latter are considered as 100%.Values are expressed as (GR T /GR C ) 100%, (CI T /CI C ) 100% and (ECI T /ECI C ) 100% in the tables [16].Toxicity was determined by evaluating S. frugiperda larval mortality and emergency adults.The assays had 20 replicates for each product tested and the control.

Statistical Analysis
Results are reported as Mean ± SD.Differences in the mean values were evaluated by analysis of variance (ANOVA).The Tukey test was used for all pair wise multiple comparisons of groups.In all statistical analysis P values > 0.05 were considered not significant.
The addition of 100 μg/g of natural ACG bis-THF 4 and 6, and their derivative analogues 5, 7 and 8 to the artificial larval diet for S. frugiperda, indicated that there were no significant toxic effects and changes in nutritional index values under assay conditions.Treatment with 9 and 10 caused significant larval mortality in the early stages (100% and 80%, respectively).Compounds 11 and 12 showed low larval mortality (20% and 15%, respectively) and high adult emergence (80% and 75%, respectively).The addition of compound 10 to the larval diet showed significant difference in consumption (CI T /CI C = 53%), decrease in larval growth rate (GR T /GR C = 33%) and inefficiency in converting the absorbed nutrients into biomass (ECI T /ECI C = 62%).The results for 11 and 12 were less significant compared to control.Natural products like these, that exert their toxicity in early instar larvae before major damage has been produced, are important.Short time ago, we investigated the mutagenic effects displayed by itrabin, as well as the phytotoxic and genotoxic action of squamocin and itrabin.Both compounds displayed slight phytotoxic and genotoxic effects on roots of Allium cepa at 2.5 µg/mL though no mutagenic effects were detected at 0.25, 0.5 and 2.5 µg/mL on Salmonella typhimurium strains TA98 and TA100 [15].
Recently, we observed the behavior in artificial membrane of POPC for mono THF ACG 1, 2 and 3, which could explain their insecticidal power on S. frugiperda, being the triacetylated 2 ACG the most toxic.The position and the amount of acetyl groups along the hydrocarbon chain had a significant effect on the activity, being three acetyl the optimal number [22].The results obtained for natural and acetylated mono THF ACG 1, 2 and 3, showed that the dehydration that occurred in the membrane by their interaction led to destabilization and this would be the main cause by which the ACG produce the mortality of S. frugiperda [17].
Structural modification of the natural ACG, blocking the OH flanking THF, allowed us to suggest the mode of action of the ACG in the membrane.In this sense, we suggest that the capacity-donor hydrogen bonding of the hydroxy group acts as a hydrophilic anchor on the surface of the liposomal membrane and this result would explain the biological activity that ACG display.We conclude that the OH groups and/or OCOCH 3 flanking the THF are essential structural factors for the intermolecular interaction with the hydrophilic polar head groups of the membrane.We suggest that this intermolecular interaction is the mechanism by which the ACG produce dehydration of the membrane and may then enhance bioactivity by restricting the location, conformation and orientation of the ACG.
These results enhance the understanding of the action mechanism of acetogenins in the membrane environment and the essential structural factors in the ACG, which makes it potentially toxic against Spodoptera frugiperda.

Table 1 .
Toxic effects and nutritional indices of ACG and their analogues on S. frugiperda.
a CI T /CI C (Consumption Index); b GR T /GR C (Growth Rate); c ECI T /ECI C (Efficiency in the Consumption Index).For comparison purposes, rates of nutritional indices are expressed as a relationship between treatment and control.ND: Not determined.