Fixation Effects on Forward and Backward Recall in a Spatial Working Memory Task

This study was designed to investigate whether fixation on the items to be remembered provided a beneficial effect on spatial working memory. A spatial working memory task based on the Corsi blocks task was assigned to 24 participants (12 men, 12 women; M age = 21.5 yr., SD = 1.3, range = 18 24) who were later asked to recall a sequence of targets in forward and backward order. When participants were asked to memorize the sequence while maintaining fixation on each target, both beneficial and disruptive effects were found in the recall performance for backward recall, but not for forward recall. Results suggest that some memory processes that proceed without fixation on the presented items are more important for backward recall.


Introduction
The capability for temporal storage and processing of spatial information, which is necessary for daily life, has been widely studied in the framework of working memory.Spatial working memory is the ability to remember a location where something is perceived and the ability to recall a series of visited locations (Vandierendonck & Szmalec, 2011).During the last few decades, a great deal of research has specifically examined spatial working memory.
A commonly used task to investigate spatial working memory is the Corsi blocks task (Milner, 1971), which is used originally as a neuropsychological assessment and which is now used also in empirical studies.In the Corsi blocks task, the experimenter taps an array of blocks sequentially.Then, the participant is asked to tap the same blocks in the same order as presented.Instead of using actual blocks, modern researchers use a compute-rized version of the task with some modification (e.g., Fischer, 2001;Smyth & Scholey, 1994).In addition, spatial working memory is often measured using the task in forward and backward recall directions (e.g., Isaacs & Vargha-Khadem, 1989;Kessels, van den Berg, Ruis, & Brands, 2008).
Several lines of research suggest that control processes involved in eye movements play a key role in maintaining spatial information in working memory (e.g., Baddeley, 1986;Postle, Idzikowski, Della Sala, Logie, & Baddeley, 2006).Most studies show that task-irrelevant eye movements interfere with spatial working memory (e.g., Lawrence, Myerson, & Abrams, 2004;Pearson & Sahraie, 2003).On the other hand, a beneficial effect of eye movement on spatial working memory was also reported.Saint-Aubin, Tremblay, & Jalbert (2007) investigated the effect of fixation duration on recall performance for forward order in a spatial working memory task.A main outcome of the study was the finding that the fixation requirement, not the fixation duration, on the items to be remembered increased recall performance across all serial positions.Furthermore, the beneficial effect of fixation was found for location recall, but not for order recall.
As described above, several lines of research report the effects of eye movement on spatial working memory.Nevertheless, the relation between spatial working memory and eye movement remains unclear.In addition, studies investigating the beneficial effects of fixation on spatial working memory have been less available.A need exists to investigate the role of eye movement on spatial working memory with various factors related to eye movement, e.g., fixation (Tremblay, Saint-Aubin, & Jalbert, 2006).Therefore, this study was conducted to examine the relation between fixation and spatial working memory; particularly, whether requiring participants to engage in fixating on the items to be remembered enhanced recall performance in a spatial working memory task.This study investigated the effects of fixation on spatial working memory not only for forward but also for backward recall.

Participants
Twenty-four undergraduate and graduate students (12 men, 12 women; M age = 21.5 yr., SD = 1.3, range = 18 -24) volunteered to participate in the experiment.All had normal or corrected-to-normal vision.The experimental protocol was administered in accordance with the guidelines of the Declaration of Helsinki and was approved by the institutional review board.Informed consent was obtained from all participants before the assessment session.No participant showed any hesitation in performing the tasks.

Materials
For the experiment, participants performed a computerized spatial working memory task based on the Corsi blocks task.At the start of each trial, a black fixation cross was presented in the center of the screen (29.27˚ × 16.71˚; 65 cm viewing distance) for 2000 msec.Subsequently, seven black squares of 1.87˚ were presented for 1000 msec, one at a time, at 500 msec intervals.Locations of the squares were selected quasi-randomly within an invisible 9 × 16 grid into which the screen was divided, with the restriction that no target area was able to overlap any other target area.For each square, the target area was a 3 × 3 grid square centered at the square.The target area was set up for the scoring of participants' responses.In addition, because previous studies reported that the number of path crossings and the spatial length of sequence affect recall performance (Parmentier & Andrés, 2006;Parmentier, Elford, & Maybery, 2005), any sequence contained no path crossings and a total of distances between subsequent squares was controlled.

Apparatus
Stimulus displays were presented on a 17-inch color monitor (1280 × 720 pixel resolution) by a core i3-based computer running SuperLab (Cedrus Corp.).Eye movements were recorded using an eye tracker (TM3; Eye-Tech Digital Systems Inc.) with QG-PLUS application (Ditect Co. Ltd.) at a sampling rate of 55 Hz.This eye tracker has an infrared camera and two infrared light sources.The accuracy for recording the eye movements was 5˚ or less.Both pupils of each participant's eyes were tracked in this study.

Procedure
Participants were tested individually in a private room.They were seated at a desk with their head rested on a chinrest, located 65 cm distant from the monitor.
After the nine-point calibration procedure, the memory task was initiated.Participants were asked to recall a sequence of seven squares in the forward or the backward order.The presentation of all target squares was completed, followed by a black screen for 500 msec.Then on a blank white screen, participants indicated the location of the targets in order using a mouse.Participants were instructed to respond as quickly and accurately as possible.Every time a participant pressed the left-hand mouse button to indicate the location, the experimenter counted aloud the number of the responses, from one to seven.No other feedback was given to the response.In each recall direction, two conditions related to eye movements were given.In the fixation condition, the participants were instructed to memorize the locations and their sequence while maintaining fixation on each target from its appearance to its disappearance.In the free condition, participants were instructed to memorize the locations and their sequence without restriction of their eye movements.Participants were tested in four blocks (2 conditions × 2 recall directions) in the memory task.In addition, in all the blocks, participants were instructed to keep their hands stationary while a sequence of targets was being presented.Two practice trials and five experimental trials were administered in each block.The order of the blocks was counterbalanced among participants.

Analysis
Participants' responses in the memory task were classified into any one of the three categories: correct recall, location error, or order error.Correct recall was a response that was within the target area at the correct serial position.A response that was outside of any target area was counted as a location error.A response was recorded as an order error if it was within a target area but at a wrong serial position.A 2 (condition: fixation and free condition) × 7 (serial position: 1 -7) repeated measures analysis of variance (ANOVA) on each category was conducted separately for forward recall and backward recall.Greenhouse-Geisser correction was applied if Mauchly's test of sphericity indicated that the sphericity assumption was violated.
Eye movements scored in the memory task were analyzed for the fixation duration.When the eye position stayed within a circle with a radius of 30 pixels over 100 msec, it was regarded as a fixation on the area.Fixation durations were computed by summing up the durations of all fixations on each target area while each target was being presented.A 2 (condition: fixation and free condition) × 2 (recall direction: forward and backward) × 7 (serial position: 1 -7) repeated measures ANOVA on fixation duration was conducted.As with described above, the Greenhouse-Geisser correction was applied if Mauchly's test of sphericity indicated that the assumption of sphericity was violated.

Correct Recall
Figure 1 shows the mean probability of correct recall: the left panel shows forward recall; the right panel shows backward recall.For forward recall, the 2 (condition) × 7 (serial position) ANOVA showed a significant effect of serial position (F 6, 138 = 7.02, p < .001;η p 2 = .23),but not of condition (F 1, 23 = 0.27, ns; η p 2 = .01),nor of interaction (F 6, 138 = 0.74, ns; η p 2 = .03).Post hoc Bonferroni tests revealed that the differences between serial position 1 and serial positions 4, 5, and 6 and between serial position 2 and serial position 5 were significant (p < .05).For backward recall, the analysis indicated a significant effect of serial position (F 4.15, 95.49 = 56.53,p < .001;η p 2 = .71),but not of condition (F 1, 23 = 4.05, ns; η p 2 = .15).However, the interaction was significant (F 4.47, 102.91 = 3.14, p < .05;η p 2 = .12).Simple main effect tests revealed significantly reduced correct recall at serial positions 1 and 3, and better recall at serial position 6 in the fixation condition relative to the free condition (p < .05).The differences among serial positions in both fixation and free conditions were also significant (p < .001).In sum, contrary to expectations, fixation on the items to be remembered did not provide a beneficial effect on the overall recall performance.In contrast, recall performance was impaired by fixating on the items to be remembered at serial positions 1 and 3 for backward recall.

Order Error
Figure 3 shows the mean proportion of order error: the left panel is for forward recall; the right panel is for backward recall.For forward recall, the ANOVA indicated a significant effect of serial position (F 6, 138 = 2.91, p < .05;η p 2 = .11),but not of condition (F 1, 23 = 0.20, ns; η p 2 = .01),nor of interaction (F 6, 138 = 1.18, ns; η p 2 = .05).Post hoc Bonferroni tests indicated that the difference between serial position 1 and serial position 5 was significant (p < .05).For backward recall, the analysis indicated a significant main effect of serial position (F 3.96, 91.14 = 28.69,p < .001;η p 2 = .56),but not of condition (F 1, 23 = 4.18, ns; η p 2 = .15).However, the interaction was significant (F 3.48, 80.09 = 2.68, p < .05;η p 2 = .10).Simple main effect tests indicated significantly increased order errors at serial positions 1 and 2 in the fixation condition relative to the free condition (p < .05).The differences among serial positions in both fixation and free condition were also significant (p < .001).The analysis indicated that the memory impairment due to fixating the items to be remembered resulted from the increment of order error.

Fixation Duration
Figure 4 shows the mean ratio of fixation duration: the left panel is for forward recall; the right panel is for backward recall.The 2 (condition) × 2 (recall direction) × 7 (serial position) ANOVA indicated significant effects of condition (F 1, 23 = 80.76, p < .001;η p 2 = .78),recall direction (F 1, 23 = 2.71, ns; η p 2 = .11),and serial position (F 3.16, 72.73 = 39.35,p < .001;η p 2 = .63),and a significant interaction between condition and serial position (F 4.64, 106.72 = 7.43, p < .001;η p 2 = .24).Simple main effect tests indicated significantly increased fixation durations at all serial positions in the fixation condition relative to the free condition.In addition, a simple main effect of serial position was significant for both fixation and free condition (p < .001),indicating that fixation durations decreased as targets were presented.This was the case even in the fixation condition.

Discussion
This study examined whether requiring participants to engage in fixating on the items to be remembered in a spatial working memory task contributed to recall performance, particularly accuracy for location.The results showed that the benefit by fixation was limited on serial position 6 for backward recall, where participants were able to recall immediately.No benefit was apparent by fixation for forward recall.Rather, for backward recall, recall performance was impaired at serial positions 1 and 3, or the latter part of recall, by requiring fixation on each target.Examination of details of the impairment revealed that order errors were increased.In sum, contrary to the literature, fixation in this study provided no apparent beneficial effect.
This inconsistent result is assumed to be attributable to the methodology in this study.In this study, participants were asked to fixate on each target from its appearance to its disappearance.Therefore, they were expected not to fixate on any other area, but the location in which the target was being presented.Their overt attention should have been allocated only to the location in which the target is presented.This restriction of overt attentional shift might disrupt memory processes, including encoding and retention.In fact, several results of studies suggest that overt or covert attentional shifts serve as a rehearsal mechanism for spatial working memory (e.g., Godijn & Theeuwes, 2012;Postle et al., 2006).In accordance with the findings, the results might reflect that fixation required in this study prevented a rehearsal among items.If a rehearsal was prevented by forced fixation, then recall performance in forward order should have become worse equally at all serial positions (Tremblay et al., 2006).Such was not the case, but if requiring fixation on each target enhanced overall recall performance to the degree described in the literature (Saint-Aubin et al., 2007), it would explain everything: because the benefit for recall performance by fixation was counterbalanced by preventing a rehearsal, it might apparently disappear for forward recall.The beneficial effect attributable to fixation is given only on location recall in the literature.Because rehearsal plays a role in maintaining order information (Smyth, 1996) as well as location information (Awh, Jonides, & Reuter-Lorenz, 1998), if a rehearsal is prevented, then order errors should have increased.However, this was also not the case, perhaps because a sequence of targets prepared in this study contain nocrossing.In such a sequence, it is less likely to confound near targets, so maintaining order information may become easier even in the fixation condition.
More importantly, for backward recall, the beneficial effect by fixation was observed at the former part of recall, but also caused impairment at the latter part of recall.This impairment suggests that the recall performance at the latter part of recall is more sensitive to a rehearsal, because the need exists to maintain information much longer to recall at the latter part of recall for backward recall.In addition, the fact that fixation on each target particularly impairs order recall suggests that sequence information is more easily influenced by the absence of a rehearsal.
However, this impaired order recall is explainable more specifically in terms of encoding.Results of some studies have suggested that the Corsi blocks task involves different memory processes for forward and backward recall: sequential processes for forward and spatial-simultaneous processes for backward recall (Cornoldi & Mammarella, 2008;Mammarella & Cornoldi, 2005).With those suggestions, spatial-simultaneous processes for backward recall may be disrupted by requiring fixation in this study because it is likely that maintaining fixation on each target leads to sequential processing of information.This sequential processing might be reflected on the typical serial position curve observed in the fixation condition for forward recall.If participants process information sequentially, not spatial-simultaneously or visually in the fixation condition for backward recall, then the results are well explained along with the finding that a binding of location and order information visually is important in the backward Corsi blocks task (Vandierendonck & Szmalec, 2004).Actually, the order error might increase because location and order information were not bound for sequential processes.
Regarding eye movement data, fixation durations during encoding in the free condition decreased as targets were presented.This result accords with those reported in the literature (Godijn & Theeuwes, 2012;Saint-Aubin et al., 2007).However, contrary to expectations, this tendency remained even in the fixation condition despite the instruction of fixation.Combined with the result that fixation durations increase at all serial positions in the fixation condition, the results suggest that some memory processes that proceed without fixation on the target presented are so important that participants are forced to sacrifice fixation durations somewhat against the instruction.To determine what memory processes indeed go in spatial working memory, it is necessary to select more appropriate paradigms and to analyze eye movements during a memory task more specifically.It would also be useful to analyze the results of saccadic eye movements, which could be not examined in this study.
In this study, a beneficial effect on recall performance by fixation on the items to be remembered was shown to a limited degree.However, if the methodological problem is eliminated, then the benefit of fixation can be demonstrated.Furthermore, the results suggest that some memory processes which proceed without fixation on the items being presented are relied upon to a greater degree for backward recall.

Figure 1 .
Figure 1.Mean probability of correct recall for forward recall (left panel) and backward recall (right panel) as a function of the serial position and condition.Error bars represent the standard error of means.

Figure 2 .
Figure 2. Mean proportion of location error for forward recall (left panel) and backward recall (right panel) as a function of the serial position and condition.Error bars represent the standard error of means.

Figure 3 .
Figure 3. Mean proportion of order error for forward recall (left panel) and backward recall (right panel) as a function of the serial position and condition.Error bars represent the standard error of means.

Figure 4 .
Figure 4. Mean fixation duration on targets for forward recall (left panel) and backward recall (right panel) as a function of the serial position and condition.Error bars represent the standard error of means.