Kudzu Response to Foliar Applied Herbicides

Chemical control is presently the most cost-effective means to control kudzu; however, some of the herbicides labeled for kudzu control have substantial non-target toxicity, poor selectivity, high cost, long soil persistence, high soil mobility and/or high use rates. The present study evaluated other herbicides for efficacy in suppressing aboveground kudzu biomass in replicated field trials at three sites over two years. A single application of aminopyralid, triclopyr or metsulfuron resulted in at least 90% kudzu suppression in the following season at two locations. After a second year of treatment those herbicides and fluroxypyr produced at least 90% kudzu suppression, and 100% kudzu control was reached on some test plots. Glyphosate, glufosinate and mesotrione were less effective in controlling kudzu. Given the rapid growth potential of kudzu, complete eradication should be pursued. None of the herbicides evaluated in the present study could reliably achieve eradication of mature kudzu with two applications, so additional control efforts would be required.


Introduction
Kudzu (Pueraria montana var. lobata) is a perennial vine that is exceptionally well adapted to the southeastern United States due to several unique physiological traits, the ability to fix atmospheric nitrogen, and the lack of significant indigenous pathogens or parasites. Recognizing this fitness, kudzu was extensively planted in the early 1900s by conservationists, farmers, homeowners and government agencies. Today, kudzu is among the worst invasive weeds in the world. It infests 3 million hectares in the United States, and more significantly, it is colonizing an additional 50,000 hectares annually. Throughout kudzu's present and expanding range, it is devastating natural ecosystems. Kudzu is an expensive nuisance to managers of utility lines. It is also a host of Asian Soybean Rust and exacerbates forest fires [1]- [3]. Just within the state of Mississippi, an estimated $54 million dollars are lost annually as a result of kudzu invasion of timber plantations [4].
Sustained overgrazing, solarization or mechanical removal can suppress kudzu, although each of these approaches presents significant challenges of its own [1] [5]. There has been a significant and ongoing search for an effective exotic [6] [7] or indigenous [8] biological control agent, but no satisfactory agent has yet been widely deployed. Thus, herbicidal control is the most commonly used management strategy.
Picloram and tebuthiuron containing herbicides are commonly recommended for kudu control. The scientific basis for these recommendations comes, in a large part, from a series of papers by Miller in the 1980s [9]- [11] and these guidelines are reflected in more recent publications (e.g., [12] [13]). Everest et al. [1] in 1999 also recommended picloram and tebuthiuron containing herbicides, but cautioned that retreatments might be necessary for up to 10 years. After evaluating 25 herbicides over 8 years, Miller [14] acknowledged that other herbicides might be warranted in some site-specific cases, but that the products other than Tordon [picloram] were less effective. Picloram, however, has several significant limitations. It is a restricted use product and requires specialized training for commercial applicators. Picloram, which is persistent in soil, is mobile in groundwater [15] and has significant non-target toxicity. Tebuthiuron is also persistent in the environment and is so non-selective that it is labeled for "total control of brush and weeds". More recently, aminocyclopyrachlor and aminopyralid have become commercially available and have been evaluated for management of kudzu [16]. Aminopyralid is especially interesting, as this herbicide is highly selective and may facilitate plant community succession to more desirable vegetation. Milestone, one commercial formulation of aminopyralid, is labeled for use on land enrolled in Conservation Reserve Program (CRP), natural areas and wildlife clearings, where few other products are explicitly permitted. The southeastern state of Mississippi, Alabama and Georgia have more than 500,000 ha of CRP [17] land and many natural or minimally managed areas that are susceptible to kudzu invasion. Other herbicides with much lower use rates, fewer use restrictions, greater selectivity and compatibility with other management approaches would be of great value in a more integrated and environmentally responsible kudzu eradication program [18].
The objective of this research was to assess the level of control of kudzu by several commercially available, selective, foliar applied, non-restricted use herbicides in controlled, replicated field trials within naturally occurring stands of kudzu.

Study Sites
Three kudu-infested sites within Mississippi, USA were selected for field research. The Eden site (33˚0'16.73"N 90˚15'56.11"W) occurs on the slopes near the edge of the Mississippi Alluvial Delta. The Mound Bayou site (33˚52'53.79"N 90˚36'44.71"W) is on a river terrace on the west bank of the Sunflower river. The Grenada (33˚55'2.00"N 89˚44'42.17"W) site is in the Holly Springs National Forest, in the northern right-of-way of Highway 7. The three plots together span a distance of about 110 km. The Eden and Mound Bayou experimental sites were initiated in 2007, and work began at the Grenada site in 2008. For consistency and reproducibility, all plots were established on flat to minor slopes with little or no competition from surrounding trees within mature kudzu monocultures. Mowed borders, 2 m wide, were maintained around each plot, which measured 2 m by approximately 15 m. These borders were mowed every 7 to 14 days during the growing season, as needed, to minimize above ground spread of kudzu between plots. Meteorological observations were collected from local weather stations and are available at http://www.DeltaWeather.MsState.Edu/.

Herbicide Application
The herbicides evaluated in this study, their commercial formulations, and application rates are listed in Table 1.
Because some products recommend the addition of spray adjuvants, Silwet L-77 was added to all products at a rate of 0.25% (v: v). All applications were made with an ATV-mounted boomless, single-nozzle system (TeeJet TFW-12) calibrated to deliver a total volume of 374 L•ha −1 via two overlapping, counter-directional passes. Each treatment was replicated on 3 plots at each location and arrayed in a randomized complete block design.

Measurement of Herbicide Efficacy
All green kudzu biomass was collected in pre-weighed bags from a 0.3 m 2 area, arbitrarily selected within each plot. In plots with very good control, multiple sampling grid placements were necessary to avoid false reporting of 100% control. The biomass was air-dried and the level of control was evaluated based on comparison to the biomass from surfactant-only control treatments. Evaluations were made at 14 days, 28 days and 11 months after the initial treatments and 11 months after the applications in the second year of treatment. Determination of the herbicidal efficacy and level of significance was via the proc mixed function of SAS v9.1 (SAS Institute, Cary, NC, USA) using a least significant difference of 0.05.

Eden Test Results
The Eden experimental location was very nearly a pure monoculture of kudzu during midsummer of 2007, as is typical of mature kudzu infestations. Fourteen days after the initial application, all of the tested herbicides produced greater that 60% brown-out (Figure 1). Greater differences emerged between treatments at 28 days after treatment. After producing some moderate initial "bleaching" symptoms, the effect of mesotrione diminished. In contrast, aminopyralid, fluroxypyr, triclopyr and metsulfuron approached 100% aboveground suppression of kudzu, with a few plots containing zero green tissue. Nearly one year after the initial treatment, those same treatments were still providing strong kudzu suppression while the effect of glufosinate was less than 10% reduction while glyphosate and mesotrione providing slightly better results. The selectivity of the herbicides was evidenced by the strong emergence of other vegetation, especially little barley (Hordeum pusillum Nutt.) and Italian ryegrass (Lolium perenne L. ssp. multiflorum (Lam.) Husnot) (data not shown). The aforementioned herbicides, with the exceptions of mesotrione and glufosinate, were reapplied to the same plots in 2008. Clopyralid was also introduced in the study. The level of control noted the following season (summer, 2009) was generally less than observed after the first season of treatment but still over 80% suppression. Clopyralid provided slightly

Percent Reduction of Green Kudzu Biomass
less reduction in kudzu aboveground biomass in its first season of application. Some possible reasons for the reduced control from the 2008 application compared to the 2007 application are year to year weather differences or that the competing vegetation in the second year prevented through herbicide spray coverage.

Mound Bayou Results
Several differences in the control of kudzu were observed at the Mound Bayou site relative to the Eden site (Figure 2). First, mesotrione and glufosinate were even less effective at Mound Bayou than the weak control they provided at Eden, with mesotrione having zero measureable effect the year following application. Also, while the level of brown-out 28 days after treatment was greater than 80 % for six of the herbicides, including glyphosate, and four individual plots had zero observed green kudzu at that time, none of the treatments averaged over 65% control the following year. During 2008 substantial competing vegetation emerged in the herbicide-treated plots, particularly Johnsongrass (Sorghum halepense) (data not shown). After the second year of herbicide application the level of control improved and the same four treatments that were most effective at Eden produced greater than 90% kudzu reduction at Mound Bayou. The plots treated with the triclopyr and fluroxypyr mixture in the first year and clopyralid in the second year had statistically similar results to the other effective treatments. Glyphosate was less effective, giving only 64% suppression after two years of application.

Grenada Results
The Grenada site has received only one herbicide application, but the results obtained to date generally support the results from the previous two locations (Figure 3). While aminopyralid and fluroxypyr were statistically less effective than metsulfuron in immediate kudzu suppression (28 DAT), all four herbicides provided 95% or better control 11 months after the initial application. While no disturbance was observed during this testing period, it is reasonable that highway maintenance crews or Holly Springs National Forest staff may have subjected this kudzu to mowing or herbicide applications or prescribed burns in previous years. These disturbances may have undermined the vigor of the kudu at this location, thus enhancing the control provided by the herbicide treatments.
Kudzu is capable of very rapid growth, and even a small, remnant, population of kudzu after treatment has the potential to negate the prior control efforts. Thus, control guidelines emphasize the importance of constant vigilance for many years after initiation of control efforts [1]. During this extended period of control efforts, it would be prudent to include products with more than one mode of action. The effective herbicides identified in the present study include an acetolactate synthase inhibiting herbicide (metsulfuron) and several pyridine carboxylic acids (aminopyralid, clopyralid, fluroxypyr and triclopyr). These classes of herbicides are presently the first and fifth highest-ranking groups of herbicides with resistant weed populations, respectively [19]. To minimize herbicide use and the concomitant likelihood of off-target pesticide movement and non-target toxicity, it might be advisable to switch to spot applications of herbicide after an initial broadcast application or including products with other modes of action. While glufosinate and mesotrione were not highly effective in controlling kudzu in the present study and are not labeled for use in any site that commonly has kudzu infestation they were included in the present study in order to consider possible novel modes of action for kudzu control. Others have evaluated combining herbicides that, while only marginally effective alone, have significant synergistic activity [20].
In addition to alternating herbicidal modes of action, herbicide resistance could also be avoided through non-herbicidal control strategies. We observed excellent kudzu suppression within the 2 m wide mowed buffers around our test plots. While the mowed buffers received reduced rates of herbicides (drift from adjacent treatments) and greater than 10 cuttings per year, we have also noted significant suppression from unsprayed areas with just a single mowing per season (data not shown). Additionally, there is an ongoing effort to develop production and application methods of the fungal pathogen, Myrothecium verrucaria [21] [22] for control of kudzu. Together, these control tactics might be integrated to increase the pressure on kudzu and reduce the time required to achieve localized eradication.
The high level of kudzu control with just a single application of metsulfuron, clopyralid and triclopyr herbicides might be somewhat surprising in the context of other published reports (e.g., [14]), which suggested that these products only provided moderate suppression. Furthermore, the effectiveness of aminopyralid and fluroxypyr has not been widely documented, and kudzu does not even appear on the list of weeds controlled by the  commercial formulation of fluroxypyr. It is possible that in the present study the local climacteric conditions were especially favorable for control; however, similar results were obtained at 3 locations over 2 years. The commercial triclopyr product evaluated in these studies, Remedy Ultra, was a new formulation, which might have improved the efficacy of this chemical. A more likely explanation, however, is the nearly ideal application conditions in the present study. In order to maximize reproducibility and control, all the plots were on fairly open, nearly level ground and all herbicides were applied with an ATV-mounted, high-volume boomless nozzle system. This methodology likely produced more complete and uniform coverage than might be routinely achieved with hand-held sprayers.

Conclusions
It can be concluded from the present study that a high level of kudzu suppression can be achieved very quickly. Although eradication of kudzu should not be expected from any of the tested herbicides with a single application and the threat from kudzu would still be present, the land could begin the transition to agriculture, forestry or natural systems. Another important conclusion is that there are several good alternatives to picloram and tebuthiuron for kudzu control, including relatively inexpensive products with greatly reduced application rates, fewer use restrictions, and greatly improved selectivity.
Mention of trade or commercial names is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USA Department of Agriculture.
Forest and the Mississippi Department of Transportation. Transline, RemedyUltra and PastureGard were provided by Dow AgroSciences. Touchdown HiTECH was provided by Syngenta.