Dietary Fiber Content of Waterleaf ( Talinum triangulare ( Jacq . ) Willd ) Cultivated with Organic and Conventional Fertilization in Different Seasons

Waterleaf (Talinum triangulare (Jacq.) Willd has long been eaten in Indonesia as vegetable and the main parts consumed are leaves and young shoots. Waterleaf is sticky presumably due to its pectin content which is associated to dietary fiber. The dietary fiber which was analyzed in the present study was influenced by cultivation practices. The aim of this research was to study the effect of organic and conventional fertilization as well as the seasonal changes to the level of TDF (total dietary fiber), IDF (insoluble dietary fiber), SDF (soluble dietary fiber), and pectic substances in waterleaf. This research was conducted in four phases: sample cultivation, sample preparation, chemical analysis, and data analysis. This research used five samples cultivated with 5 different compositions of organic fertilizers and vice versa for conventional fertilizers. The cultivation was done in the experimental field of University Farm, Bogor, Indonesia during rainy season and dry season. Samples were harvested 8 weeks after planted, dried using drying oven for 17 hours at 60 ̊C, ground and filtered to 40 mesh; and kept at −10 ̊C until analysis. The overall result showed that the conventional samples contain higher dietary fiber than the organically fertilized samples, except the pectic substances of plant in dry season. Although its IDF content is higher than the SDF, the SDF content of waterleaf is relatively high compared to other vegetables, especially in dry season. N. Andarwulan et al. 335


Introduction
Waterleaf (Talinum triangulare (Jacq.)Willd) has long been eaten in Indonesia as vegetable and the main parts consumed are leaves and young shoots.It contains a sticky substance that is presumably due to its pectin content which is associated to dietary fiber.Waterleaf is also an important source of phenolic antioxidants [1].The dietary fiber which was analyzed in the present study as influenced by cultivation practices.
Fertilization may be applied in organic and conventional practices which have been reported to influence a broad range of chemical constituents.Several studies have shown higher polyphenol content [2]- [5] and antioxidant activity [6] in organic plants compared to conventional grown plants.Furthermore, trends of higher content of vitamin C, iron, magnesium, and phosphorus [7] and sugar [8] and lower levels of nitrates [2] [9] have also been reported.As plants grow, nutrients in soil are reduced, therefore the addition of fertilizer in the form of organic or conventional is important to maintain the soil fertility.
Another cultivation practice that will affect plant growth and chemical constituents is the selection of the growing season, such as a rainy or dry season.For example, dietary fiber content in plants has been associated to seasonal changes [10] [11] while the synthesis of secondary metabolites has been associated to environmental changes, including temperature changes between day and night, rainfall, drought, and sunlight intensity [12]- [14].Reference [14] reported that waterleaf dry season crops showed high content of phenolic compound but low level of PAL (phenylalanine ammonia lyase) and low level of chlorophyl.This suggest that phenolic compounds were synthesized through the malonic acid pathway rather than the phenilpropanoid.This pathway uses acetyl coenzyme A as a precursor, causing substrate competition with the chlorophyl synthesis pathway, since both are using the same precursor.As the chlorophyl level decreased in the dry season, the photosynthesis process was affected decreasing the products of photosynthesis and affecting the synthesis of other compounds, such as dietary fiber.
Thus, in the present study, we characterized the dietary fiber constituents of waterleaf as influenced by cultivation practices including a comparison between organic and conventional fertilization as well as the influence of a rainy and dry growing season conditions.

Sample Cultivation
Samples used in this research included 5 samples cultivated with organic fertilizer (Table 1) and 5 samples cultivated with conventional fertilizer (Table 2).The plants were cultivated in the different seasons, i.e. rainy season (February-April) and dry season (May-July).The plants were harvested after 8 weeks, it was taken three replication for dietary fiber and pectic substances analysis to obtain representative data.
Dung contains N level of 1.29% with 71% of moisture content in wet basis.Guano contains P level of 10.43% in the form of P 2 O 5 with 8.69% of moisture content, and husk ash contains K level of 1.10% in the form of K 2 O.The dose of each element (N, P, and K) was obtained by multiplying the amount of fertilizer (kg/ha) in each treatment with the percentage of each element, except for the n element, there was a slightly different calculation because the fertilizer has moisture vontent of 71%.Example of calculations in organic treatment 1: • dose of N element: (100 − 71)% × 1.29% × 6.1 ton/ha = 22.82 kg/ha; • dose of P element (in the form of P 2 O 5 ): (100 − 8.69)% × 10.43% × 75.6 kg/ha = 7.20 kg/ha;

Sample Preparation
Edible portion of the sample, which is about 15 cm from the top was obtained from the harvested 8 weeks old plants.Then, samples selection was performed as an initial step to obtain representative and uniform samples.The sample were cleaned and divided into wet samples and dried samples.The moisture content of wet samples was then analyzed.Most of the other samples were then dried with drying oven for 17 hours at 60˚C.Dried samples was ground to 40 mesh to obtain powder leaves.Water content, dietary fiber, and pectic substances of the dried sample were then analyzed.Moisture content of fresh and dried samples are determined by oven method [15].

Dietary Fiber Analysis
Total dietary fiber content analysis was determined using the gravimetric method [16].All procedures are also performed on the blank to see if there are deposits of non-fibers derived from reagents or enzymes remaining in the residue and can be counted as dietary fiber.About 0.5 mg of sample was weighed in 200 ml of beaker glass.Then, 25 ml of phosphate buffer pH 6.0 was inserted into the beaker glass, pH value was adjusted to 6.0 ± 0.2.After that, 0.05 ml of termamyl added.Then, the beaker glass was closed using aluminum foil paper (alufo) and was placed in boiling water for 15 minutes, shaken slowly in every 5 minutes.The heating time can be added until 30 minutes to reach an internal temperature between 95˚C -100˚C.Subsequently, the solution was cooled at room temperature, pH value was adjusted to 7.5 ± 0.2 with NaOH 0.275 N.Then, 2.5 mg of protease was added into the sample.Protease can also be used in the form of solution (50 mg in 1 ml phosphate buffer), was pipetted as much as 0.05 ml and was put into the sample just before used.
Recovered sample was then incubated for 30 minutes at 60˚C with continuous agitation.The sample was cooled and was added with 5 ml HCl 0.325 N, pH value was measured to 4.0 -4.6.If the pH value has not reached, more acid can be added.A 0.15 ml of amyloglucosidase enzyme (AMG) was added, and then the sample was recovered with alufo, was incubated for 30 minutes at 60˚C with continuous agitation.A 140 ml of ethanol 95% which had previously been heated to 60˚C (volume was measured after heating) was added to form the precipitation.Sample was left at room temperature for 60 minutes.The precipitate was then filtered quantitatively through the crucible.Previously, the crucible and its celite were weighed to the accuracy close to 0.1 mg.
The residue was washed with 3 × 5 ml of ethanol 78%, 2 × 5 ml of ethanol 95%, and 2 × 5 ml of acetone, respectively.Some samples can form resin. Filtration may be assisted by stirring using a spatula.The time needed for washing and filtration can be varied from 0.1 to 6 hours, the average time taken is 20 minutes per sample.The length of time can be reduced by using vacuum suction during filtration.Crucible containing the residue was then dried overnight in a drying oven at 105˚C, and then it was cooled in desiccators and was weighed to the accuracy reached 0.05 mg.To obtain residue weight, subtract with the weight of crucible and celite.
Residue analysis of one replicate was then used for protein analysis using the Kjeldahl method.The conversion factor used is N × 6.25.Another sample test was then burned for 5 hours at 475˚C to obtain ash weight, and then cooled in desiccators and was weighed to the accuracy close to 0.1 mg.Subtract of crucible and celite to obtain ash weight.
The procedure performed for insoluble dietary fiber analysis [17] was closely similar with total dietary fiber analysis until quantitatively sample filtration step into the crucible.Subsequently, the residue was washed with 2 × 5 ml of water (to dissolve SDF), 2 × 5 ml of ethanol 95%, and 2 × 5 ml acetone, respectively.The steps for drying crucible until the final stage of the procedure are similar to the total dietary fiber.Determination of soluble dietary fiber was done by subtracting the content of total dietary fiber with the content of insoluble dietary fiber.

Pectic Substances Analysis
Pectic substances is calculated based on colorimetric method of [18] which has been modified by [19].Anhydrogalacturonic obtained from the hydrolysis of the substances together with o-hydroxydiphenyl will produce color that can be measured at 520 nm.

Data Analysis
Data are presented as the mean ± standard deviation of at least triplicate determinations.Statistical significance was by one-way ANOVA and student t-test, with p value ≤ 0.05 considered significant.

Dietary Fiber Content and Growing Season Effects
In conventional fertilized waterleaf, TDF showed distinct ranges between the rainy and dry season crops ranging in values of ~74 -81 and 39 -44 g/100g dw, respectively.Similar trend was observed for IDF between rainy and dry season crops with range values of ~69 -76 and 26 -28 g/100g dw, respectively, however, this trend was reversed in SDF showing range values of ~4.6 -5.8 and 13 -17 g/100g dw, respectively.In general there was no clear trend between the different doses used in conventional fertilization and the observed values corresponding to TDF, IDF and SDF (Table 3).
When comparing the average values of all conventional fertilized treatments we observe that values for TDF and IDF in rainy season crop > dry season crops (p < 0.05).However, for SDF values results indicate that rainy season crops < dry season crops (p < 0.05) (Figure 1).
On the other hand, in organic fertilized waterleaf, TDF also showed distinct ranges between the rainy and dry season crops ranging in values of ~63 -79 and 36 -42 g/100g dw, respectively.Similar trend was observed as well for IDF between rainy and dry season crops with range values of ~59 -74 and 25 -28 g/100g dw, respecttively, however, once again this trend was reversed in SDF showing range values of ~4.4 -4.7 and 11 -15 g/100g dw, respectively.Like in conventional fertilization there was no clear trend between different doses used in organic fertilization and the observed values corresponding to TDF, IDF and SDF (Table 3).
When comparing the average values of all organic fertilized treatments we observe that values for TDF and

Pectic Substances Content and Growing Season Effects
In conventional fertilized waterleaf, pectin substances showed distinct ranges between the rainy and dry season crops ranging in values of ~3.6 -5 and 5.4 -6.2 g/100g dw, respectively.Similar trend was observed in organic fertilized waterleaf between rainy and dry season crops with range values of ~3.1 -4 and 5.7 -7.4 g/100g dw, respectively.In general there was no clear trend between different doses used in conventional or organic fertilization and the observed values corresponding to pectin substances (Table 4).When comparing the average values of all conventional and organic fertilized treatments we observe that values for pectin substances in rainy season crop < dry season crops (p < 0.05) (Figure 1).
In general, the average moisture content of Talinum triangulare (Jacq.)Willd in the present study was 90.39%, which is within the range of 90% -92% previously reported for waterleaf (Rifai, 1994;Mensah et al., 2008).Since waterleaf is consumed fresh, we present the values for TDF, IDF, SDF (Table 5) and pectin substances on fresh weight basis as well, which show similar trend as those described above for conventional and organic fertilization based on dry basis (Table 4).

Comparison of Dietary Fiber and Pectin Substances Content in Organic and Conventional Fertilized Waterleaf
When comparing the average values of all conventional and organic fertilized treatments we observe that for TDF, IDF and SDF, the values in conventional crops > organic crops (p < 0.05).The only exception is IDF in dry season crops where there was no difference between both methods production (p > 0.05) (Figure 1).When comparing the average values of all conventional and organic fertilized treatments for pectin substances, we observe that values in conventional crops > organic crops (p < 0.05) in rainy season while the opposite trend in dry season (p < 0.05) (Figure 1).

Discussion
During a dry season, plants experience water stress due to the limited rainfall and high light intensity.Mualim   [14] reported that waterleaf in dry season have significantly lower chlorophyll content than the rainy season possibly due to a low supply of nutrients (including N) and substrate competition.Thus, the substrate for complex compound like dietary fiber may decrease causing low levels of dietary fiber such as TDF and IDF in the dry season waterleaf as observed in the present study.Another adaptation mechanism of plants in response to abiotic stress is by undergoing osmotic adjustment.This is done by accumulating compatible solutes, such as sucrose, amino acid (proline and glycine betaine), sorbitol, mannitol, and inositol and its derivatives [20]- [22].The transgenic tobacco (Nicotina tabacum) that accumulates D-ononitol showed less photosynthesis inhibition in salinity and water stress condition [22].Inositol, D-ononitol, and D-pinitol protect the plant by protecting the cell structure againts radical oxygen species (ROS) and controlling the cell turgor pressure [22].Inositol is a precursor of dietary fibers.Inositol will be oxydized into UDP-D-galacturonic and other sugar UDP, like UDP-glucuronic and UDP-L-arabinose [21].The glucuronic compound is a precursor of many soluble dietary fiber like hemicellulose, gum, mucilage, and pectin.The inositol accumulation will possibly lead the plants to synthesize more SDF, including pectic substances, in dry season waterleaf compared to rainy season crops as observed in the present study.The proportion of dietary fiber varies among many vegetables, affected by some factors including level of maturation, part of plant to be consumed, and cultivation practices [23].Compared to other common vegetables in Indonesia, TDF and IDF contents of waterleaf dry season crops are relatively low (Table 6) whereas TDF and IDF contents in waterleaf rainy season crops are above most vegetables.When consumed regularly, waterleaf may contribute with a significant amount of dietary fiber to the diet.FDA recommends a daily diet containing 25 g of dietary fiber for adult women and 38 g for adult men.The present study shows that 100 g of waterleaf dry season crops will supply ~3.89 -4.09 g of dietary fiber and contribute to fulfill ~10% -16% of TDF needs and ~9% -13.9% of IDF needs per day.On the other hand, 100g of waterleaf rainy season crops, with its high TDF and IDF content, will contribute to fulfill ~17% -26% of TDF needs and 21.3% -32.6% of IDF needs per day.
In relation to SDF, waterleaf dry season crops contain the highest SDF content compared to most vegetables (Table 6).In previous reports, a meta-analysis on 67 studies focusing on SDF, showed that there was a significant reduction in serum cholesterol with increased dietary fiber intake [24].It was further reported that 2 -10 g consumtion of dietary fiber per day can reduce total serum cholesterol and LDL-cholesterol concentration.Accordingly, 200 g of waterleaf dry season crops could supply ~2.8 g of SDF, which is within the range needed to potentially reduce blood cholesterol levels.Thus, the recommended daily consumption of waterleaf is 200 g fresh weight/ day with serving size of 100 g fresh waterleaf.From dry season waterleaf, this amount will fulfill 20% -32% of TDF daily needs and 36% -55% SDF daily needs.We need to combine with other food to fulfill the dietary fiber needs completely.
In relation to pectin, waterleaf dry season crops are a rich source of pectin substances.Compared to the pectin level of many fruits and vegetables [25], the pectin level in waterleaf rainy season crops (0.58 -0.63 g/100g fw) is higher than pectin levels in longan fruit (0.34 g/100g fw), raspberry (0.34 g/100g fw), apple (0.39 -0.49g/100g fw), legumes (0.43 -0.63 g/100g fw), orange (0.57 g/100g fw), and sweet potato (0.61 g/100g fw) among others.Several studies have reported that pectin as part of SDF has the ability to reduce blood cholesterol [26]- [28].Baker [28] reported in in-vivo studies that pectin levels of 0.23 g/100g from citrus orange mixed into the diet could reduce LDL level of rats by 5% and decrease the glucose response.Thus, waterleaf due to its high pectin content could be considered a good source of pectin substances for these biological activities.

Conclusions
In the present study, we reported that season growing conditions for waterleaf had a large effect on TDF, IDF, SDF and pectin substances content.For example, waterleaf rainy season crops had larger levels of TDF, IDF than waterleaf dry season crops, while the latter had higher levels of SDF and pectin substances than the former.
On the other hand, despite that there were statistically differences between conventionally and organically fertilized waterleaf crops in relation to TDF, IDF, SDF and pectin substances, these differences were minimal and would not make a major difference in the contribution to the diet.
According to our results, diets which could include 100 -200 g of waterleaf rainy season crops would significantly contribute to the recommended levels of daily intake of TDF and IDF, while 100 -200 g of waterleaf dry season crops would significantly contribute to the recommended levels of daily intake of SDF and pectin substances.

Figure 1 .
Figure 1.Dietary fiber profile of waterleaf that were cultivated using organic and conventional fertilization at different season (a) TDF; (b) IDF; (c) SDF; and (d) pectic substances.

Table 1 .
Treatment of organic fertilizer.

Table 2 .
Treatment of conventional fertilizer.
a -fSamples with same letter in the same group of treatment indicate that they were not significantly different at α = 0.05.
a -fSamples with same letter in the same group of treatment indicate that they were not significantly different at α = 0.05.

Table 6 .
Dietary fiber content of common vegetables in Indonesia.