Ceria-Based Mixed Oxide Supported CuO: An Efficient Heterogeneous Catalyst for Conversion of Cellulose to Sorbitol

A series of CeO2-Al2O3, CeO2-TiO2, CeO2-ZrO2, and CeO2-SiO2 mixed-oxide supported copper catalysts were prepared by a modified deposition-precipitation method from ultra dilute aqueous solutions and were investigated for hydrogenolysis of cellulose in aqueous medium, in the presence of hydrogen to produce sorbitol as major product. Among all the catalysts tested in the present work, CuO/CeO2-ZrO2 catalyst proved to be the most promising with high conversion (92%) and excellent selectivity (sorbitol 99.1%), at an intermediate reaction temperature of 245 ̊C in a neutral aqueous solution without an aid of liquid phase acid. The catalyst was recyclable in repeated runs and no deactivation was observed even after five reaction cycles. CuO/CeO2-ZrO2 has been characterized by XRD, SEM, TPR and BET surface area techniques.


Introduction
The rapid depletion of petroleum reserves and growing concerns about global climate change have attracted immense attention towards alternative fuel production pathways based on renewable resources, such as biomassderived carbohydrates [1].Cellulose is one of the most abundant sources of biomass and is a polymer of D-glucose with glucose units joined together through β-1,4-glycosidic bonds.The abundant functional groups (OH groups) of cellulose make it an ideal feedstock for sustainable production of chemicals [2].Nevertheless, the extensive hydrogen-bonding networks and the high crystallinity of cellulose make it resistant to chemical and enzymatic degradation.Great efforts have been devoted to the cellulose transformation in various ways, such as fermentation with enzyme, photochemical conversion, hydrolysis with acid catalysts, pyrolysis at high temperatures, and etherification with alcohols [3]- [10].All these conventional methods for conversion of cellulose to value added products have some serious drawbacks such as slow reaction rate, difficult separation of products and catalysts, corrosion hazards, harsh conditions and generation of large amount of neutralisation waste [11].Heterogeneous catalysis has advantages to overcome these drawbacks.Conversion of cellulose with solid catalysts (heterogeneous catalysts) under hydrothermal conditions is a green and efficient process for the sustainable production of chemicals, in which several consecutive reactions proceed in one reactor and cooperate well.Hydrogenolysis of cellulose resulting in C-C and C-O cleavage is a promising technology for the direct conversion of biomass into commodity chemicals and therewith, an entry point for future biorefinery models [12]- [14].Surprisingly, few studies on hydrogenolysis of cellulose exist and were carried out at rather high reaction temperatures.Current investigations aim at the development of solid heterogeneous catalysts to overcome the need for costly homogeneous catalysts for conversion of cellulose to commodity chemicals [15].Hydrolysis of cellulose to glucose followed by hydrogenation of glucose to sorbitol in single pot using a bifuntional heterogeneous catalyst seems an advanced method.
Sorbitol is used as a sweetener in diet foods and pharmaceutical tablets because of its low calorie value.It is a potential raw material for the synthesis of variety of value-added chemicals such as isosorbide, 1,4-sorbitan, glycols, glycerol, lactic acid and L-sorbose [16].Isosorbide is used to increase the glass transition point of polyethylene terephthalate (PET) which helps to store hot drinks in PET bottles.L-sorbose is a precursor to vitamin C [17].Dumesic and co-workers have reported that hydrogen for fuel cell could be produced from sorbitol over supported metal catalysts with high selectivity compared with glucose [18].The C5-C6 hydrocarbons are also formed from sorbitol [19].Sorbitol can also be fermented to ethanol via enzymatic dehydrogenation [20].Thus, sorbitol is selected as one of the 12 top value-added products from biomass in the US DOE report [21].
In continuation of our work on environment friendly chemical transformations [21]- [26], we herein, report copper supported on ceria-zirconia as a highly stable catalyst for direct and selective hydrogenolysis of cellulose to sorbitol.To the best of our knowledge, this catalytic system has not been reported yet for the hydrgenolysis of cellulose and is one of the best performances of heterogeneous catalysts ever reported.
CeO 2 found extensive applications in catalysis, in particular due to its reducible character, and improved surface metals dispersion [27].The redox chemistry (Ce 3+ /Ce 4+ ) of doped cerium oxides is sensitive to crystal structure defects [28].The replacement of cerium ions by cations of different size and/or charge modifies ionic mobility within the lattice resulting in the formation of a defective fluorite structured solid solution [29].The acidity of ceria mixed oxides also increases with the addition of cations like Zr 4+ thus helps in the breakdown of cellulose to its monomers [30].This concept was used to prepare different ceria based mixed oxide supports for copper catalyst for one hydrogenolysis (hydrolysis followed by hydrogenation).The reaction scheme of cellulose hydrogenolysis is illustrated in Scheme 1.

Catalyst Preparation
The catalyst was prepared in two steps.
The first step involves preparation of catalyst supports and to accomplish this reported method was followed [25] [26].A solution containing Ce(NO 3 ) 3 The second step involves impregnation of copper oxide on to the support for the preparation of the catalysts like Cu/CS, Cu/CZ, Cu/CT and Cu/CA.To impregnate the copper oxide (10 wt%), adequate amounts of aqueous Cu (NO 3 ) 2 •3H 2 O solutions were used.To this aqueous solution, the finely powdered mixed oxide (support) was separately added.The excess water was gradually evaporated on a hot-water bath under constant mechanical agitation and subsequently dried at 110˚C for 12 h.Finally, the obtained materials were calcined at 500˚C for 5 h in a closed electrical furnace.

Catalytic Performance Tests
Avicil cellulose (500 mg), 10 wt% Cu/CZ (200 mg), and distilled water (50 mL) were charged in a 1 L stainless steel Parr 4521M (Moline, IL) pressure reactor controlled with a Parr 4843 temperature controller equipped with an overhead stirrer and a pressure transducer.It was pressurized with H 2 gas of 750 PSI.The reactor was heated to 245˚C and maintained at this temperature with constant stirring at 1000 rpm for 240 min.After completion of the reaction, the reactor was cooled to room temperature, and the liquid part was separated by centrifugation, and then filtered through whatman filter paper.The solution containing the water-soluble products was analyzed by HPLC with Hypersil NH 2 5 μm column at 25˚C, Shimadzu LC-20AT pump, and Shimadzu RID-10A detectors at 40˚C.Acetonitrile/water solution (75/25 V/V) was used as mobile phase at 0.8 mL/min.Sorbitol and manitol were characterized by comparing their HPLC profile with the standard samples and their percentage concentration in each sample was calculated from the peak area distribution (HPLC graph) using external standard method.The conversion of cellulose was determined from the weight difference of the solid residue after the reaction.After finishing the reaction with the fresh catalyst, the reaction mixture was centrifuged and the residue was separated from the liquid part.The residue, which contained the recovered catalyst and the un-reacted cellulose, was used for the next reaction cycle without any treatment.

Catalyst Characterization
The X-ray diffraction spectrum (XRD) of the catalyst (Cu/CZ) was taken on a Rigaku Miniflex diffractometer, using Ni-filtered Cu Ka (0.15418 nm) radiation source.The sample was scanned over the range 2.00 -79.99 on 2θ scale with steps 0.011˚ and step time 13.6 s.SEM of the sample was carried out using SEM.Hitachi S-520 Japan instrument.The reducibility and BET surface area of catalyst was determined by CHEMBET-3000 TPR/ TPD/TPO instrument, containing a quartz reactor (i.d. 4 mm) and a TCD detector.XRD patterns of the Cu/CZ in Figure 1 shows small diffraction peaks at 2θ = 35.6˚,38.9˚ and 61.5˚ are attributed to the crystalline copper oxide (tenorite copper oxide, ICDD PDF 45-0937) [31].The diffraction lines at 2θ = 28.9˚,33.5˚, 47.9˚, 57.3˚, 69.8˚ and 77.9˚ correspond to Ceria.Zirconia shows humps at 33.9˚, 38.8˚, 58.2˚ and 71.0˚ merged with diffraction peaks of Ceria which can occur because of incorporation of zirconium cations into the ceria lattice leading to shrinkage of the lattice [32]- [34].
To study the morphology SEM of sample (Figure 2) was carried out using SEM.Hitachi S-520 Japan instrument.The SEM image is a confirmation for coarse surface (thus elevated surface area), which is able to adsorb substrate and or reagent to a high extent.It is observed that CuO particles are randomly distributed over the support surface.It should also be noted that CuO over the support constitutes several aggregates and not a continuous film.The surface morphology of CuO supported on CeO 2 -ZrO 2 shows that no separate crystallites of the bulk phase of CuO were found in the supported sample.
CuO distribution on supported samples surface was analysed by ICPMS method.The analysis was repeated on different samples in order to ensure the reproducibility of the obtained results.Microanalytcal data of ICPMS analysis show that the amount of CuO deposited on the support is 8.9 wt%.
To assess the reducibility of the dispersed oxide and the interaction between the supported metal and the support, TPR was performed.This is an important technique for understanding the reduction behavior and thermal   3).The H 2 consumption patterns reveal that the dispersed copper oxide over the support reduces at a relatively lower temperature than pure CuO.When copper oxide reduces to a certain extent, it tends to dissociate H 2 much more easily and then reduces at a higher rate, which is a potential source for the TPR peak at 160˚C.As noted from previous reports, it is not easy to resolve the sequential reduction peaks of copper ( 22

Cu
Cu CuO ) [35]- [37].However, the observed different features in the TPR profile could be related to structural/ morphological variations in the copper oxide species.In this respect, the TPR profile obtained clearly reveals a higher copper heterogeneity, in agreement with other characterization results described above.
Thus, the presence of several reduction peaks for CuO/CeO 2 -ZrO 2 must be related to the presence of different copper species in the sample.Avgouropoulos [31], studying the reduction of CuO supported on ceria, attributed a peak at 170˚C to the reduction of highly dispersed CuO strongly interacting with the ceria surface and two peaks at 235˚C and 275˚C to the reduction of larger CuO particles weakly interacting with ceria.A broad reduction peak with a shoulder at low temperature may be attributed to the reduction of highly dispersed copper oxide species interacting with ceria, and a peak at higher temperature assigned to the reduction of bulk-like CuO [38].
To know the surface area of the catalyst Nitrogen adsorption and desorption was determined at −196˚C by means of an automated CHEMBET-3000 adsorption apparatus.Samples were pretreated in high vacuum at 200˚C for 2 h.The specific surface area of Cu/CZ was found to be 112.51m 2 /g.

Cellulose Hydrogenolysis
At the onset of this work, we first screened the different ceria based mixed oxide supported copper catalysts, for conversion of cellulose to sorbitol.The copper loading in all these catalysts was maintained as 10 wt%.Though the conversion of cellulose was not satisfactory (due to un-optimized conditions at the beginning), to our surprise the sorbitol was found as major product with very high selectivity in all the samples (Table 1, entry 1-4).Among the screened catalysts Cu/CZ was found to be the most active to give 18% conversion with 99.9% selectivity of sorbitol and 0.1% mannitol (Table 1, entry 4) followed by Cu/CS, Cu/CT and Cu/CA.
We proceeded with Cu/CZ for further studies and screened this catalyst with different copper loading.10 wt% loading copper on CZ was found to show the optimum results.The conversion of cellulose was found to increase with the increase of catalyst amount from 150 to 300 mg (Table 2).The selectivity of sorbitol was low with 300 mg of catalyst and maximum selectivity was obtained by using 250 mg of catalyst with a little decrease in the cellulose conversion.This decrease in sorrbitol selectivity with increase in catalyst amount may be due to the degradation of the sorbitol under excess catalyst conditions.
The influence of reaction temperature on the catalytic activity of Cu/CZ was investigated at constant reaction time to find the optimum conditions for the conversion of cellulose to sorbitol (Table 3).Normally, elevated temperature can contribute to the enhancement of reaction rate and conversion efficiency.We observed that the reaction temperature plays an important role in the reaction process.At 190˚C of reaction temperature 11% of cellulose conversion was observed with 99.991% sorbitol selectivity (Table 3, entry 1).With increase in temperature, conversion of cellulose was found to increase significantly.The optimum temperature thus found was 245˚C at which 62% conversion of cellulose was observed with 99.971% of sorbitol selectivity (Table 3, entry 3).Increasing temperature above 245˚C could not bring any major increase in cellulose conversion, e.g. at 260˚C the conversion of cellulose remained at 62% with a slight decrease in sorbitol selectivity and above this temperature decomposition of sorbitol was observed (Table 3, entry 4).
It is a well known fact that time plays a great role in chemical transformations.In some reactions the yield as well as the selectivity of the desired products increase with increasing reaction time, but sometimes prolonged reaction time leads to the decomposition of the desired product and formation of unwanted side products.At all the above optimized condition we studied the effect of time on the conversion of cellulose and the sorbitol yield.The conversion of cellulose and sorbitol yield increased quickly with increasing the reaction time from 30 min to 240 min.92% cellulose conversion with 99.081% sorbitol selectivity was obtained at reaction time of 240 min (Table 4).No further increase in the cellulose conversion was observed upon increasing the reaction time to 300 min, instead sorbitol content in the sample was found to be lowered to 96.092% with the extended reaction time (Table 4, entry 4).Hence the optimum reaction temperature and time was set to 245˚C and 240 min respectively.
Pre-reduction of the catalyst was found to have slight effect on the conversion as well as the sorbitol selectivity (Table 5, entry 2).Conversion of cellulose was found to increase by 3% with 99.992% sorbitol yield, but this activity of the reduced catalyst retained for the first cycle only and remaining cycles gave results almost similar to unreduced Cu/CZ.Since it was difficult to pre-reduce the used catalyst in each cycle, we studied the recyclability of the unreduced catalyst only.
Some of the recently reported catalyst systems and their comparison with that of our finding are illustrated in Table 6.In comparison to the reported results, this method seems much better in terms of cellulose conversion as well as selectivity.In addition to that some of these reported methods require costly catalysts (Table 6, entry 1, 3) and others give very low selectivity (Table 6, entry 2, 5).We observed the degradation of cellulose when the reaction was conducted without using a catalyst.When only CZ support material was used as catalyst, we observed 58.9% conversion of cellulose and the reaction mixture contained 34.7% of glucose along with some unidentified products.Evidently, the CZ itself presented notable catalytic activity for hydrolysis of cellulose into sugars.According to Gutierrez-Ortiz et al., strong acid sites markedly increases upon addition of zirconia to the ceria lattice [39].Interestingly current investigations showed that the combination of cellulose hydrolysis and hydrogenation can significantly improve the conversion of cellulose.These results were found consistent with the results reported by Fukuoka et al. [11] [20].Thus conversion of cellulose to sorbitol occurs in two steps in a single pot.In the first step the cellulose is converted to glucose which is water soluble.The cleavage of the glycosidic linkages is achieved via addition of water catalyzed by acidic support.In the second step the hydrogenation takes place in presence of catalyst Cu/CZ and major product obtained is sorbitol.This appears to be catalyzed by supported metals via dissociation of hydrogen which may spillover on the surface of the support, migrate to Lewis acid sites and release an electron to form protonic acid sites.These sites could consequently act as active sites for acid-catalyzed hydrolysis of cellulose as suggested by Fukuoka et al. [11] [20].As per this mechanism the results obtained for the optimization of support are in agreement with the acidity of their supports.So decreased conversions of cellulose obtained by using catalyst other than Cu/CZ are due to the lower acidity of the supports which provide lower hydrolysis compared to CeO 2 -ZrO 2 .The selectivity of sorbitol is almost similar in all the catalysts used, thus the support of catalyst, time of reaction, amount of catalyst and reaction temperature can be said to have major effects on supported copper catalyzed hydrogenolysis of cellulose to sorbitol.

Recyclability of the Catalyst
The promising activity of the Cu/CZ catalyst encouraged us to further investigate its stability during repetitive runs.Long-term stability and reusability of the heterogeneous catalyst are extremely important characteristics for future industrial application to reduce production cost substantially.The recyclability of the catalyst was checked for the cellulose conversion.After the reaction was finished, the spent catalyst was separated from the liquid substance, and reused in a new experiment for the reaction under the same reaction conditions.It was found that the catalyst can be recycled at least 5 times.When the catalyst was reused no obvious decrease of the sorbitol yield was observed in the second run onwards, which indicates the stability of the catalyst.

Conclusion
We have developed highly active and robust Cu/CeO 2 -ZrO 2 catalyst for the direct and selective transformation of cellulose to sorbitol.The high activity of support for cellulose hydrolysis, the mesoporous structure of CZ facilitating reactant transportation, and the enhanced hydrogenation activity of Cu catalyst are responsible for the excellent performance of Cu/CZ.By efficiently coupling the hydrolysis reaction and hydrogenation of cellulose, the high yield sorbitol was obtained.This strategy might provide guidance for the cellulosic biomass conversion to polyols in near future.

Figure 2 .
Figure 2. SEM of Cu/CZ.stability of the active metal oxide particles.The reducible character of catalyst was determined by CHEMBET-3000 TPR/TPD/TPO instrument, containing a quartz reactor (i.d.= 4 mm) and a T.C.D. detector.The H 2 TPR curve of the catalyst is depicted in (Figure3).The H 2 consumption patterns reveal that the dispersed copper oxide over the support reduces at a relatively lower temperature than pure CuO.When copper oxide reduces to a certain extent, it tends to dissociate H 2 much more easily and then reduces at a higher rate, which is a potential source for the TPR peak at 160˚C.As noted from previous reports, it is not easy to resolve the sequential reduction peaks of copper (2
NO 3 ) 3 •9H 2 O following the procedure similar to that for the preparation of Ceria-Silica.All solid supports are in the 1:1 molar ratio for Ce 2 O 3 :M 2 O 3 .

Table 1 .
Effect of catalyst on conversion and sorbitol selectivity.

Table 2 .
Effect of catalyst amount on the cellulose conversion.

Table 3 .
Effect of temperature on sorbitol selectivity.

Table 4 .
Effect of time on the conversion of cellulose.

Table 5 .
Effect of pre-reduction on activity of Cu/CZ.

Table 6 .
Comparison of Cu/CZ with reported systems.