Reversible Immobilization of Chelating Affinity Surfactants on Reversed Phase Adsorbents for Protein and Peptide Separations under Metal Affinity Chromatography

Alkyl-bound silica was modified using chelating surfactants and the resulting adsorbent was used in immobilized metal affinity chromatography of proteins and peptides. Brij-76, a non-ionic amphiphilic surfactant with an alkyl moiety and an ethylene oxide chain, was reversible adsorbed to alkyl silica (C18). The hydroxyl group at the end of the ethylene oxide chain was chemically modified previously with an iminodiacetate functionality as chelating agent of transitional metal ions. Cu(II) was studied as immobilized ion for the adsorption of peptides and proteins. Three chromatographic supports were prepared having different Cu(II) capacities. For a low Cu(II) capacity case, the generated adsorbent behaved as a controlled access media preventing the adsorption of large molecular weight proteins, such as BSA, while small peptides, such as Angiotensin III, or amino acids could be retained. For a medium and high Cu(II) capacity, the synthesized adsorbent no longer behaved as a controlling access media and all molecules in this study, either large or small, were retained by the immobilized ion. Nonetheless, most of the BSA was strongly retained by the system and a pH change did not remove any of the adsorbed BSA while the small molecules were removed by the same pH change.


Introduction
Alkyl silica, a common adsorbent for reversed phase chromatography of biological macromolecules [1], has been previously modified with surfactant molecules that adsorb hydrophobically forming hemi-micelles or bilayered structures as depicted in Figure 1 [2].Several applications of similar modified adsorbents have emerged such as a generation of mixed mode adsorbents with hydrophobic and ion exchange properties [3]- [6], the fractionation of proteins by size exclusion using hydrophobic silica coated with non-ionic surfactants [7], the use of modified surfactants with affinity ligands, ion exchange groups or dyes at the end of the polyethylene glycol residue for protein purifications [8]- [11], the analysis of drugs using an alkyl-bound silica saturated with surfactants (Tween or Brij) [12], and the use of a polyoxyethylene-type non-ionic surfactant as a media for hydrophobic interaction chromatography [13].
During the creation of alkyl silica saturated with a surfactant that inhibits the interaction of a protein with alkyl residues, a surfactant containing a polyethylene glycol residue is necessary.Polyethylene glycol (PEG), either covalently grafted or hydrophobically adsorbed, has shown to prevent protein adsorption onto surfaces [12] [14].Despite the PEG rejection properties, surfactants modified at the end of the PEG residue with an adsorption site are able to retain proteins during chromatographic procedures as previously described.
The recovery of proteins by immobilized metal affinity chromatography (IMAC) is widely known after the work of Porath et al. [15].IMAC is primarily based on the interaction between histidine residues on the surface of a protein with immobilized metal ions present on a stationary phase through a coordination process.This coordination process is reversible and thus, proteins can be selectively purified after metal ion recognition by imidazole or pH gradients.Iminodiacetic acid as chelating agent and Cu(II) and Ni(II) as immobilized ions are by far the most employed for protein purification [16].
Chelating surfactants have been used to immobilize proteins on chromatographic beads.Ho et al. [17] in their approach they coated polystyrene beads with a Pluronic surfactant modified with a chelating agent loaded with a transitional metal ion and the resulting adsorbent was used for the immobilization of luciferase on the polystyrene beads.
Here we describe the synthesis of a chelate-derivatized surfactant using Brij derivatives and iminodiacetic acid (IDA) to form surfactant-IDA complexes and the reversible adsorption of these surfactant-chelates to octadecyl-bonded silica.Octadecyl-bound silica beads were coated with the surfactant-IDA derivatives and after loading with Cu(II) at different densities, used to study protein adsorption by IMAC interactions.

Instrumentation
Chromatographic analyses were performed using a Gilson HPLC system (USA) equipped with two isocratic pumps, a mixer, a manual injection valve (with a 0.5 mL sample loop), a UV-Vis detector, and a fraction collector.The system was controlled by the Unipoint software from Gilson.The chromatographic column was a glass column from Amersham Biosciences (USA) having an internal diameter of 0.5 cm and a length of 5 cm.Synthesis of the chelating-surfactant derivative was performed using a Parr (USA) mini reactor with 4843 controller.

Synthesis of Chelating-Surfactants
The synthesis of chelating surfactants was performed by a three-step reaction scheme.In step 1, as an example, 10 g of Brij-76 were melted at 55˚C under vacuum to remove water for 1 h.Afterwards 5.2 mL of thionyl chloride were added under a nitrogen atmosphere and the temperature was increased to 65˚C.Chlorination continued for 6 h.Then vacuum was applied to remove unreacted thionyl chloride for 2 h.The product was dried overnight at 50˚C in an oven.In step 2, 40 mL of absolute ethanol were used to dissolve the modified surfactant and the solution was added to a reactor containing 400 mL of ammonia-saturated ethanol.The reactor was closed and the temperature raised to 100˚C, the reaction was let to proceed for 4 h.The mixture was then cooled to room temperature (RT) and concentrated under vacuum.The aminated residue was finally dried overnight at 50˚C in an oven.In step 3, the resulting solid was dissolved in 40 mL of hot ethanol (70˚C) and 2.05 g of bromoacetic acid (in 60 mL of ethanol with 2.16 mL of 10 M NaOH) were added.The temperature was raised to 80˚C and the reaction proceeded for 14 h controlling the pH in the range 8 -9 adding 10 M NaOH.The temperature was then lowered to RT and the solvent was eliminated under vacuum.The residue was suspended in 200 mL of water, acidifying the mixture to pH 1 -2 by adding concentrated hydrochloric acid.The resulting aqueous suspension was first extracted with ethyl acetate and then with chloroform.The organic solvent was evaporated and the residue dried in an oven at 50˚C overnight.The involved reactions during the surfactant-chelating synthesis are depicted schematically in Figure 2. The TNBS test was used to determine the efficiency of steps 2 and 3.

Surfactant Quantification
Surfactant concentration in the native and chelating derivative was determined by the Bradford method [18] in which 0.2 mL of sample was mixed with 0.8 mL of protein reagent and the absorbance of the mixture was measured at 610 nm.

Adsorbent Preparation
Octadecyl-bound silica was packed in a glass column (0.5 cm ID) to a height of 2.7 cm using methanol at a flow rate of 2 mL/min.The column was then washed with DI water to remove the alcohol.The interaction and saturation of the silica matrix with the chelating surfactant was carried out by feeding the surfactant solution at a flow rate of 0.5 mL/min until saturation.This was followed with a washing step with DI water to remove unbound surfactant.Afterwards, the column was equilibrated with a 10 mM Cu(II) solution.Unbound Cu(II) ions were also removed with DI water.

Determination of Cu(II) Capacity on the Silica-Chelating-Surfactant Matrix
The Cu(II) capacity in the prepared adsorbents was measured as follows.A glass column packed with surfactant modified adsorbent was fed with a 10 Mm Cu(II) solution at a flow rate of 0.5 mL/min until saturation.Afterwards, DI water was fed to the column to remove unbound copper ions from the tubing and from the void volume in the column.The entire process was followed by measuring the absorbance of the eluate at 825 nm.Once the breakthrough and washing curves were obtained, Cu(II) capacity was measured by the mass balance expressed in Equation ( 1).
where c m is the measured Cu(II) concentration (mM), c m0 is the fed Cu(II) concentration (mM), t is the time (min), t f is the Cu(II) solution feeding time (min), t w is the final time of experiment (min), F is the flow rate (mL/min), and V is the column volume (mL).

Surfactant Saturation Capacity of Octadecyl-Bound Silica
The surfactant capacity of the reversed-phase adsorbent was measured by the same method used for Cu(II) capacity, with the quantification of surfactant concentration on the eluate by the Bradford method as previously described.A similar equation to Equation (1) was then used to estimate the amount of surfactant retained by the alkyl silica.

Protein and Peptide Adsorption Studies
Once the alkyl-bound silica was modified with the chelating surfactant and loaded with Cu(II) ions, the column was equilibrated with buffers B and A at a flow rate of 0.5 mL/min.Buffer A consisted of 20 mM PO 4 , 500 mM NaCl, pH 7.0 while buffer B was 100 mM PO 4 , 500 mM NaCl, pH 4.0.
Once the system was equilibrated with buffer A (adsorption buffer), a 0.5 mL sample was injected.Absorbance of the eluate was followed at 220 or 280 nm.After a washing step with the same buffer A, buffer B (desorption buffer) was fed to remove specifically retained molecules.

Surfactant and Cu(II) Capacity of Modified Adsorbents
The surfactant capacities on the reversed-phase columns for native and modified Brij-76 were determined.For the modified surfactants, the Cu(II) capacity was also determined and the results are shown in Table 1.The surfactant capacity on the reversed-phase matrix for the native molecule was similar to the capacities obtained by Sing et al. [12] for Brij-76 on C 18 Davisil (109 mg/mL) and on C 18 Ultrabase (104 mg/mL).The adsorption of these amphiphilic surfactants on hydrophobic silica, in general, changes the polarity of the adsorbent since the polyethylene glycol moieties are exposed to the aqueous solvent.The surfactant adsorption from an aqueous solution above the critical micelle concentration on an alkyl residue is schematically depicted in Figure 4. Since the test C 18 silica has a pore size of 30 nm, while dimensions of the Brij surfactants lay in the range 2 -5 nm [19], the surfactant molecules are expected to enter the pores of the adsorbent and adsorb at the alkyl moieties of the silica reducing the surface area and pore volume while preserving the pore shape [20] [21].Once the surfactants are adsorbed on the alkyl silica surface they can form hemispherical structures called hemi-micelles as has been suggested by Rosen [22].
The measured Cu(II) capacities for chelating surfactants were comparable with Cu(II) capacities from commercial IMAC systems reported in the literature [23].Two of the surfactant-IDA derivatives Brij-76-IDA 1 (low Cu(II) capacity) and Brij-76-IDA 2 (medium Cu(II) capacity) were obtained from the ethyl acetate liquid-liquid extraction procedure while a third derivative Brij-76-IDA 3 (high Cu(II) capacity) was obtained from the chloroform extraction procedure.Since ethyl acetate is slightly less polar than chloroform, it seems to have preference for the unmodified surfactant which is expected to be less polar than the IDA-modified surfactant.
The HLB value for the original Brij-76 is reported in Table 1, this value implies that this surfactant has slightly favored hydrophilic properties.The introduction of the iminodiacetic acid functionality in the Brij-76 molecule is expected to increase these hydrophilic properties.This situation could explain the slightly lower surfactant capacity between Brij-76-IDA 3 and Brij-76.

Protein and Peptide Adsorption Studies: Plain C18 Silica
Figure 5 presents the chromatograms from pulse analyses of BSA (2 mg/mL), Angiotensin III (0.05 mg/mL), Trp (0.04 mg/mL), and His (0.03 mg/mL) dissolved in DI water with 0.1% TFA.The elution of the material adsorbed by the octadecyl groups attached to the silica matrix was achieved by feeding acetonitrile with 0.1% TFA.Both BSA and Angiotensin III samples were almost completely retained by the plain C 18 silica while 30% of the His sample was not.Most of the Trp sample that was injected is only retarded on the plain C 18 silica, only a small amount was retained hydrophobically and later recovered by the organic solvent.Trp and His where poorly retained by the plain C 18 silica due to their hydrophilic behavior.The same results shown in Figure 5 were obtained (data not shown) after removing the surfactants using ACN recovering plain C 18 silica.

Protein and Peptide Adsorption Studies: Brij-76-IDA 1 Derivative
Once the C 18 silica was saturated with Brij-76-IDA 1 and equilibrated with the adsorption buffer, pulses of BSA (1 mg/mL), RNAse A (1 mg/mL), Angiotensin III (0.05 mg/mL), Trp (0.05 mg/mL) and His (0.03 mg/mL) were injected separately for analysis.The chromatograms are shown in Figure 6.In this case the system was not   loaded with Cu(II) ions and thus, none of the test molecules were retained.The proteins or peptides left the column with the void volume.The molecules were not retained by ion exchange processes either since the adsorption buffer had a high ionic strength to inhibit electrostatic interactions.These results corroborate that the adsorbent has changed polarity due to the presence of the ethylene oxide chain, changing from a hydrophobic to a hydrophilic adsorbent.Moreover, the test molecules are being excluded from the hydrophobic surface by the widely known rejecting properties of polyethylene glycol [24].The alkyl silica (C 18 ) was not accessible even for small molecules such as His.A similar behavior was obtained by Chang [7] where glycyl-tyrosine was not retained hydrophobically by diphenyl silica covered with Tween.Two theories have been considered towards the molecular description of the interaction between PEG and proteins for the creation of non-fouling surfaces.The first theory considers that the PEG layer induces a steric repulsion of proteins associated with an entropic repulsion originated from the compression of the PEG layer [25] [26].The second theory considers that the affinity of water molecules towards oxygen atoms in the PEG chains is high enough to avoid protein interaction [24].The PEG water affinity relies on the conformation of the polymers that offers two hydrogen bond acceptors in ideal distance for hydrogen bonding with water [27].

Protein and Peptide Adsorption Studies: Brij-76-IDA 1 -Cu(II), Low Cu(II) Capacity
In this case the Brij-76-IDA 1 -saturated C 18 silica was equilibrated with Cu(II) ions and with desorption and adsorption buffers.The modified adsorbent was equilibrated with the desorption buffer to remove weakly bound Cu(II) ions.Pulses of BSA and RNAse A were tested and the resulting chromatograms are shown in Figure 6.Unexpectedly, BSA was almost completely unretained since none of the protein was recovered upon application of the desorption buffer (see Figure 6(a)).Only a small amount (7% of the amount injected) of RNase A was recovered with the desorption buffer (see Figure 6(b)).BSA and RNAse A are known to have affinity towards immobilized Cu(II) ions by iminodiacetic acid [28] [29].Since the chemical modification of the surfactant introduces an iminodiacetic acid functionality at the end of the ethylene oxide chain, Cu(II) ions should be exposed to the protein present in the aqueous buffer.Thus, these Cu(II) ions should be able to retain the test proteins.
The observed non-retention of proteins could be associated to several factors.First it is necessary to consider the structure of the adsorbent which is silica modified polymerically for the incorporation of the alkyl chains.Once the surfactant is adsorbed on these alkyl moieties, and with the fact that the number of surfactant molecules bearing an IDA functionality is less than the native surfactant, the probability that an IDA-modified surfactant is buried by the presence of native surfactant is high and thus, Cu(II) ions are not accessible for protein retention.Another possibility is the consideration of the hemi-micelle formation in which the IDA-modified surfactant is closer to the surface and it is being protected by the ethylene oxide chains of the native surfactants that complete the hemi-micelle.Finally, the reduction in pore size has to be considered since this effect will make difficult for the proteins to enter the adsorbent.
Another factor that could be considered is the expected distribution of molecular weight oligomers present with the surfactant Brij-76.This surfactant theoretically contains 10 ethylene oxide units but the reaction product will also contain, in large proportion, surfactant molecules with higher or lower ethylene oxide units according to a Poisson distribution [30]- [32].Considering for example, a Poisson distribution with 10 ethylene oxide units, only 12.5% of the molecules will be present as Brij-76.Molecules with 9 ethylene oxide units will be likely present in the same percentage (12.5%)while molecules with 11 units will represent 11.4%.Thus, it is possible that if the surfactant molecules with 10 or less ethylene oxide units are being preferentially modified to bear an iminodiacetic acid moiety and if these modified surfactant molecules are closer to the surface and somehow protected by the longer unmodified surfactant molecules, the observed behavior could be explained.
By considering any of these possibilities, a small peptide or protein could be more easily retained since the native surfactant will only exert a minor influence on their adsorption.To test the latter concept, Angiotensin III, Trp, and His were injected to the system and the results are shown in Figure 6.All these molecules were retained by the immobilized Cu(II) ions and desorbed by a pH change necessary to disrupt the associated chelation.Angiotensin III, Trp and, His have been demonstrated to possess affinity towards immobilized Cu(II) ions on IDA [33].
According to the previous results, it was considered to determine if mixtures of large molecules with small molecules could be separated to corroborate a potential restricted access situation.Thus, mixtures of BSA (0.5 mg/mL) with Angiotensin III (0.025 mg/mL) or His (0.015 mg/mL) were injected to the system to determine the retention properties of the modified adsorbents.The results for these experiments are shown in Figure 7 where it is clear that only a small (almost negligible) amount of BSA is retained while the small molecules are completely retained and thus, they are being separated from the large molecular weight protein.The latter represents an extraordinary result since a restricted access chromatographic medium was created using common C 18 silica as support.

Protein and Peptide Adsorption Studies: Brij-76-IDA 2 -Cu(II), Medium Cu(II) Capacity
Alkyl silica was saturated with Brij-76-IDA 2 and after equilibration with the adsorption buffer pulses of BSA (1 mg/mL), RNAse A (1 mg/mL), Angiotensin III (0.05 mg/mL), Trp (0.05 mg/mL), and His (0.03 mg/mL) were injected and the chromatograms obtained were similar to the results presented with the alkyl silica saturated with Brij-76-IDA 1 (data not shown).As expected, none of these test molecules were retained by the system.Now the system was loaded with Cu(II) ions and pulses of the test molecules were performed.Desorption of adsorbed molecules was accomplished by a pH change.Despite the presence on an unretained peak for the injection of BSA (Figure 8), it represents only a small amount of the total protein injected (the absorbance of the eluate was followed at 220 nm).The amount of BSA that was recovered from the pH change was negligible.From these results it is clear that the system is strongly retaining BSA most likely due to multipoint interactions between surface histidines and immobilized Cu(II) ions.It is also clear that this result is derived from the increase of Cu(II) capacity by the modified surfactant.A pulse of RNAse A was also performed and 63% of the total injected protein was retained and recovered by a pH change.The increase in Cu(II) capacity is clearly increasing the amount of RNAse A that is being retained (it increased from 7% to 63%).Pulses of Angiotensin III, Trp, and His were also performed and the three substances were retained by the Cu(II) ions and recovered with a pH change (Figure 8).An increase in Cu(II) capacity increased the probability of exposed Cu(II) for protein or peptide interaction and thus, the adsorption of BSA and RNAse A was now possible.The schematic preparation of the adsorbent for the retention of peptides or proteins with Cu(II) ions is depicted in Figure 9.
Also, mixtures of BSA with Angiotensin III or His were injected to determine if the small molecules continued to be recovered by a pH change while BSA remains adsorbed on the chromatographic system.Both the peptide and the amino acid were successfully separated from the large molecular weight protein (data not shown).

Protein and Peptide Adsorption Studies: Brij-76-IDA 3 -Cu(II), High Cu(II) Capacity
The chloroform fraction of the reaction mixture from the surfactant modification recovered a highly IDA-subs-  tituted Brij-76.A clean silica C 18 was saturated with this surfactant and with a Cu(II) solution.After Cu(II) saturation, the original silica support changes from white to bright blue upon Cu(II) complexation.Pulse studies of BSA, RNAse A, Trp and His were performed on this system and the results are comparable to the system using the derivative Brij-76-IDA 2 -Cu(II).None of the proteins or the amino acids were retained with the system lacking Cu(II) ions (data not shown).

Conclusions
The surfactant Brij-76 was successfully modified to bear an iminodiacetate group at the end of the ethylene oxide chain.This modified surfactant was strongly adsorbed by its hydrophobic tail to silica C 18 .The leakage of surfactant from the silica C 18 was minimal once the surfactant molecules were not present on the mobile phase.The adsorption of surfactant to the silica C 18 clearly changed the polarity of the original support that originally presents adsorption of peptides or proteins by hydrophobic interactions.Once the surfactant was loaded with Cu(II) ions, the generated support served as a stationary phase for immobilized metal affinity chromatography and peptides and proteins were retained through interactions with the immobilized ions that were disrupted by a pH change allowing the recovery of some of the studied molecules.
Depending on the Cu(II) capacity of the attached surfactant (or in the number of surfactant molecules bearing an iminodiacetate group), the generated adsorbent could prevent the adsorption of large molecular weight proteins allowing the interaction of small peptides and amino acids with the immobilized ions creating thus, a restricted access media.Once the Cu(II) capacity was increased, all the studied molecules (having large and small molecular weights) were retained by the modified adsorbent.When BSA was retained by interaction with the immobilized ions, its recovery by a pH change was not possible revealing a high affinity likely derived from multipoint interactions between histidine residues from the surface of BSA and the Cu(II) ions.
Brij-76 was successfully modified to bear an iminodiacetic moiety.First the efficiency of Brij-76 amination was quantified using the TNBS test.This test revealed that approximately 60% of the original Brij-76 molecule was aminated.The same TNBS was used to determine the absence of amino groups in the Brij-76 molecule after carboxymethylation.The chemical structures of the native and the chelating surfactants are shown in Figure 3.

Figure 9 .
Figure 9. Adsorption of proteins using immobilized Cu(II) ions supported by a chelating surfactant deposited over alkyl silica of Brij-76 on C 18 silica.

Table 1 .
Cu(II) and surfactant capacities for studied adsorbents.