In Vitro Anticancer Activity of Plant-Derived Cannabidiol on Prostate Cancer Cell Lines

Cannabinoids, the active components of Cannabis sativa Linnaeus, have received renewed interest in recent years due to their diverse pharmacologic activities such as cell growth inhibition, anti-inflammatory effects and tumor regression, but their use in chemotherapy is limited by their psychotropic activity. To date, cannabinoids have been successfully used in the treatment of nausea and vomiting, two common side effects that accompany chemotherapy in cancer patients. Most non-THC plant cannabinoids e.g. cannabidiol and cannabigerol, seem to be devoid of psychotropic properties. However, the precise pathways through which these molecules produce an antitumor effect have not yet been fully characterized. We therefore investigated the antitumor and anti-inflammatory activities of cannabidiol (CBD) in human prostate cancer cell lines LNCaP, DU145, PC3, and assessed whether there is any advantage in using cannabis extracts enriched in cannabidiol and low in THC. Results obtained in a panel of prostate cancer cell lines clearly indicate that cannabidiol is a potent inhibitor of cancer cell growth, with significantly lower potency in non-cancer cells. The mRNA expression level of cannabinoid receptors CB1 and CB2, vascular endothelial growth factor (VEGF), PSA (prostate specific antigen) are significantly higher in human prostate cell lines. Treatment with Cannabis extract containing high CBD down regulates CB1, CB2, VEGF, PSA, pro-inflammatory cytokines/chemokine IL-6/IL-8. Our overall findings support the concept that cannabidiol, which lacks psychotropic activity, may possess anti-inflammatory property and down regulates both cannabinoid receptors, PSA, VEGF, IL-6 and IL-8. High CBD cannabis extracts are cytotoxic to androgen responsive LNCaP cells and may effectively inhibit spheroid formation in cancer stem cells. This activity may contribute to its anticancer and chemosensitizing effect against prostate cancer. Cannabidiol and other non-habit forming cannabinoids could be used as novel therapeutic agents for the treatment of prostate cancer. Corresponding author.


Introduction
Prostate cancer is the most commonly diagnosed malignancy in men and the second leading cause of cancer death in males.Most early tumours are androgen-dependent, thus depriving the tumour of androgens via surgical or medical castration [1] has proven to have significant effects at the initial stages of prostate cancer.Despite the early efficacy of androgen ablation, advanced prostate cancer is resilient to such treatments and eventually relapses into a hormone refractory (androgen-independent) disease, with devastating results on morbidity and mortality rates [2].There is currently no curative treatment against hormone refractory prostate cancer (HRPC).The most effective treatment regime for patients with HRPC, docetaxel-based chemotherapy, can only improve the median survival time by 3 months [3].Therefore, effective treatment strategies against metastatic HRPC are urgently needed.The current therapies are not only successful in targeting differentiated tumor cells but also sparing the putative cancer stem/progenitor cells [4].Similar to normal stem cells, cancer stem cells (CSCs) are thought to be quiescent compared with mature cancer cells [5].This property makes CSCs resistant to chemotherapeutic drugs, which mainly target actively replicating cells.In addition, two recent studies demonstrated that prostate CSCs are androgen independent [6] and may not respond to hormone ablation as mature tumor cells do.Owing to their ability to self-renew and differentiate, CSCs are capable of regenerating the heterogeneous tumor population (with both androgen-dependent and androgen-independent cells) after hormone ablation, which accounts for tumor relapse.Therefore, elimination of the bulk of frequently replicating tumor cells as well as the rare subset of slow, dividing stem-like cells that are responsible for tumor regeneration may represent a better therapeutic strategy in the treatment of prostate cancer.
The therapeutic properties of the hemp plant, Cannabis sativa, have been known for many years, but the recreational use of its psychoactive effects has restricted its possible pharmaceutical application.In recent years cannabinoids and their derivatives have drawn renewed attention because of their diverse pharmacologic activities such as cell growth inhibition, anti-inflammatory effects, and tumor regression (Guzman et al. 2003) [7].Cannabinoids have been shown to induce apoptosis in gliomas [8], PC-12 pheochromocytoma [9], CHP100 neuroblastoma [10], and hippocampal neurons [11] in vitro, and most interestingly, regression of C6-cell gliomas in vivo [12].Plant-derived cannabinoids, especially cannabidiol, are shown to be potent inhibitors of prostate carcinoma both in vitro and in vivo [13].Induction of apoptosis by cannabinoids in prostate and colon cancer cells may be phosphatase dependent [14].Our case studies confirmed cannabinoid (CB) efficacy in reducing muscle spasticity in multiple sclerosis [15], pain levels over a 12-month period [16] and Cannabis responsive head injury induced multiple disabilities [17].
To date, cannabinoids have been successfully used in the treatment of nausea and vomiting [18], two common side effects that accompany chemotherapy in cancer patients.Nevertheless, the use of cannabinoids in oncology might be somehow underestimated since increasing evidence exist that plant, synthetic, and endogenous cannabinoids (endocannabinoids) are able to exert a growth inhibitory action on various cancer cell types.However, the precise pathways through which these molecules produce an antitumor effect has not been yet fully characterized, also because their mechanism of action appears to be dependent on the type of tumor cell under study.It has been reported that cannabinoids can act through different cellular mechanisms, e.g., by inducing apoptosis, cell-cycle arrest, or cell growth inhibition, but also by targeting angiogenesis and cell migration [7] [19].Furthermore, the antitumor effects of plant, synthetic and endocannabinoids can be mediated by activation of either CB1 [20] or CB2 receptors or both [21] [22].After the discovery of the two specific molecular targets for THC, CB1, and CB2 [23], it became clear that most of the effects of marijuana in the brain and peripheral tissues were due to activation of these two G-protein-coupled cannabinoid receptors.However, evidence is also accumulating that some pharmacological effects of marijuana are due to Cannabis components different from Tetrahydrocannabinol (THC).Indeed, C. sativa contains at least 400 chemical components, of which 66 have been identified to belong to the class of the cannabinoids [23].The main limitation of the possible future use of THC in oncology might be represented by adverse effects principally at the level of the central nervous system, consisting mostly of perceptual abnormalities, occasionally hallucinations [24].However, most non-THC plant cannabinoids seem to be devoid of direct psychotropic properties.In particular, it has been ascertained that cannabidiol is nonpsychotropic [25] [26] and may even mitigate THC psychoactivity by blocking its conversion to the more psychoactive 11-hydroxy-THC [27].Moreover, it has been recently found that systematic variations in its constituents (i.e.cannabidiol and cannabichromene) do not affect the behavioral or neurophysiological responses to marijuana [28].Finally, it has been also shown that, unlike THC, systemic administration to rats of cannabigerol does not provoke poly-spike discharges in the cortical electroencephalogram during wakefulness and behavioral depression [29].These and other observations reinforce the concept that at least cannabidiol, cannabigerol, and cannabichromene lack psychotropic activity and indicate that for a promising medical profile in cancer therapy, research should focus on these compounds, which so far have been poorly studied with regard to their potential antitumor effects.By keeping this goal in mind, we decided to investigate the antitumor properties of plant cannabis.We screened two distinct chemically characterized Cannabis extracts (enriched in high cannabidiol and low THC), where the presence of nonpsychotropic cannabinoids along with THC has been reported to mitigate the potential side effects of the latter compound in clinical trials [27].
Despite research efforts, little is known about the prostatic cancer stem cells which were suggested to play an important role in tumor initiation, progression, and chemoresistance [6].Because CSCs are believed to contribute to chemoresistance, we reasoned that the chemosensitizing effect of cannabis may be mediated through targeting of prostate CSCs.In this study, we show that cannabis extract treatment significantly down regulates protein expression of prostate CB1 and CB2.Meanwhile, Cannabis treatment not only suppresses the spheroid formation ability of prostate cancer cells but also down regulates pro-inflammatory cytokine IL-6 and chemokine IL-8, decreases secreted protein and mRNA expression of prostate-specific antigen and VEGF levels.Our overall findings support that cannabidiol (CBD) may possess anti-inflammatory, anti-CSC effects, and down regulate both cannabinoid receptors CB1 and CB2, leading to their anticancer and chemosensitizing effect against prostate cancer.

Chemicals and Reagents
Cannabinoid reference standards for THC, CBD and CBN were purchased from Sigma.For extractions, HPLC grade methanol, formic acid and acetonitrile were purchased from Fischer scientific.All reagents were at least of analytical grade.Stem cell reagent with supplements was purchased from Stem Cell technology, Vancouver.ELISA kits for PSA were purchased from (Roche Diagnostics PSA, Indianapolis, IN).

Pre-Treatment of Cannabis Samples in the HPLC Method
The cannabis extracts were kindly provided by Green Cross society of BC, Vancouver.Plant material samples were dried for 24 h in a 35˚C forced ventilation oven.Crumbly samples were then ground and mixed.200 mg of this fine powder was weighed in a flask and extracted with 10 mL of a methanol by agitation during 30 min.The extract was filtered and diluted hundred times with methanol for running on HPLC equipment.Under these conditions suitable chromatography is achieved for the three most abundant cannabinoids present in the samples.

HPLC Equipment and Chromatographic Conditions
Fifty samples of cannabis were analyzed by HPLC, out of which two were selected with high CBD and low THC.All chromatographic runs were carried out using a Waters 2695 HPLC with a 996 Photodiode array Detector.Full spectra were recorded in the range 228 -300 nm.Chromatographic separations were achieved using a Waters XTerra® MS C18 analytical column (2.1 × 150 mm column).Equipment control, data acquisition and integration were performed with Empower Pro 2.0 software.The mobile phase at 1 ml/min.consisted of a mixture of water, Acetonitrile and 1% Formic acid.Initial setting was 55% acetonitrile (v/v), which was linearly increased to 85% acetonitrile over 30.5 min, then increased to 95% over next 2 min and 100% over next 0.5 min.After maintaining this condition for 2.5 min, the column was set to initial condition in 0.5 min and re-equilibrated to 55% for another 4.5 min.The total runtime was 40 min.Flow-rate was set to 0.3 ml/min, the injection volume was 10 µl.All experiments were carried out at 30˚C.Calibration curves were run for a number of com-mercial standards (Sigma).Two cannabis extracts with high CBD and low THC were also run on 5890 series 11 Gas chromatograph from Hewlett Packard.Typical chromatograms with peaks showing THC, CBD and CBN are shown in Figures 1(a)-(c).

Cell Lines and Culture Conditions
LNCaP, DU145, PC3, BPH parental and PNT1B parental cells were obtained from American Type Culture Collection (Manassas, VA).PC3, DU145, BPH parental and PNT1B parental cells were cultured in DMEM supplemented with 5% fetal bovine serum.LNCaP were maintained in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 5% fetal bovine serum (Invitrogen).Primary cultures of human dermal fibroblasts (Lonza, Walkersville, MD, USA) were cultured in DMEM supplemented with 5% fetal bovine serum.All cell types were kept at 37˚C in a 5% CO 2 environment.

Treatment of Cells
Cannabis (dissolved in DMSO), was used for the treatment of cells.For dose-dependent studies, cells were treated with cannabis at final concentrations of 0.5 -7 µl/ml of medium (Figure 4 for concentration of THC, CBD and CBN in µg/µl).We also included a control treated with 0.5 -7 µl/ml of DMSO.It was established that DMSO had no effect on the growth of the spheroids for 24 or 48 hours.

Cell Viability Assay
Cytotoxic profiles of high CBD extracts were assessed using the MTS™ [(3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium) and an electron coupling reagent (phenazinemethosulphate; PMS)] cell viability assay (Promega, Madison, WI, USA).Briefly, 5000 cells seeded in wells of 96 well plates (Corning, NY, USA) were allowed to attach overnight, exposed to 0.5 to 7.0 μl/ml cannabis extract for 24 h.To make sure that the vehicle was not affecting cell functions, dimethyl sulfoxide (DMSO) (Sigma) was added to control experiments.The tetrazolium dye was added to each well of the plate and incubated for a further 4 h.Optical density was then measured at 490 nm using a ELX 808 Automated Microplate Reader (Bio-TEK® Instruments, Winooski, VT, USA).All treatments were performed in triplicate.The percentage change in viable cell mass was presented as the OD 490 ratio between the untreated and treated cells at the indicated concentrations.

Lipopolysaccharide-Stimulated Cytokine-Related Factorprofiling
Dermal fibroblasts were grown in 12 well plates (Corning) to produce confluent monolayers in DMEM.LPS was added to the culture media at 1 μg/ml for 24 h ± 45 or 20 μg/ml CBD cannabis extract.Conditioned media was harvested for ELISA tests.In data not shown, we observed that medium alone, with or without an equivalent volume of DMSO, and cell-free supernatant derived from control, uninfected cells exhibited indistinguishable levels of basal cytokine/chemokine production.

Enzyme-Linked Immunosorbent Assays
Sandwich ELISAs for IL-6 and IL-8 (RayBiotech, Norcross, GA, USA) were performed on culture supernatants from the indicated treatments according to manufacturer's instructions.Standard curve was constructed with supplied standards to allow conversion of OD 450 absorbance readings of experimental samples to pg/ml.All samples were assayed in triplicate.
The cycler was programmed with the following conditions: a) initial denaturation at 94˚C for 2 minutes, followed by 35 cycles of b) 94˚C for 40 seconds; c) annealing of the primer template at 58˚C for 40 seconds; and d) extension at 72˚C for 40 seconds.Target gene expression was normalized to 18Srna levels in respective samples as an internal standard and relative transcript quantity was calculated using the ΔΔCt method of Applied Biosystems [30].Each assay was performed in triplicate.
The calculating equation for ΔΔCt is as follows:

ELISA for PSA
The human PSA ELISA kit was used for the quantitative determination of PSA levels in culture medium on a Cobas e 411 immunoassay analyzer (Roche Diagnostics PSA, Indianapolis, IN), according to the manufacturer's instruction.This kit uses a technique of quantitative sandwich immunoassay for determination of PSA with an estimated sensitivity of 1 ng/ml PSA antigen.

Quantification of Apoptosis by Flow Cytometry
The cells were grown at density of 1 × 10 6 cells in 100 mm culture dishes and were treated with Cannabis extract (0.5 -7 µl/ml) for 24 hours.The cells were trypsinized, washed with PBS and fixed with ice cold 70% ethanol.Cells were subsequently stained with a propidiumiodide (PI) staining buffer (0.1 mg/ml RNase A, 0.05% Triton-X-100 and 50 µg/ml of PI in PBS) for 1 hour.Samples were analyzed by flow cytometer and BD FACSDiva Software.

Spheroid Formation Assay
Briefly, cells were trypsinized, washed with 1% PBS and re-suspended in DMEM with 5% FBS.Five hundred cells were added to each well of a 6-well plate (Ultra low Cluster plate, Corning NY 14831).Cells were grown in Stem cell medium with proliferation medium supplemented with heparin and hydrocortisone from Stem cell Technology, Vancouver.Fresh medium with the above supplements was added every week.Cannabis extract (20 µg/µl -60 µg/µl CBD) was added to the medium and the number of spheroids was counted at the Day 14 of the assay.Each experiment was repeated in triplicate, and each data point represents the mean and SD.

Statistical Analysis
MTS assay: Differences in cell viability, expressed as the OD ratio between treated and untreated cells at the indicated concentrations, were calculated using a Student's two-tailed t-test assuming equal variances.Enzyme-linked immunosorbent assays: Results were compared using a one-way analysis of variance followed by a Bonferroni post-test comparing only the pairs of interest.All analysis of variance p-values were significant, and the post-test results are shown in the respective tables.

Expression of Cannabinoid Receptors in Normal and Prostate Cancer Cells
We first compared the expression levelsof both cannabinoid receptors CB1 and CB2 in Prostate epithelial cells (PrEC) and a series of human prostate cancer cells.The data shown in Figure 2 revealed that expression of both CB1 and CB2 receptors was significantly higher in prostate cancer cells, CB1, 23 fold for LNCap, 90 fold for DUI45, 570 fold for PC3 as compared with normal prostate cells BPH1 and PNT1B PrEC cells (Figure 2(a)).CB1 for LNCaP was further upregulated by 841 fold when stimulated with androgen R1881 (Figure 2(b)).CB2, 105.8 fold for LNCap, 45 fold for DUI45, 71 fold for PC3 as compared with normal PrEC cells (Figure 2(c)).CB2 for LNCaP was further upregulated by 1683 fold when stimulated with androgen R1881 (Figure 2(d)).

Effect of Cannabis Extract on Cell Viability of PrEC (BPH and PNT1B), LNCaP and PC3 Cells
To evaluate the cell viability response of cannabis extract on PrEC, LNCaP and DU145 cells, MTS assay was employed.Data in Figure 3

CB1 and CB2 Receptor Activation Signals Growth Inhibition in LNCaP Cells
To study the possible implication of CB1 and CB2 receptors in cannabis extract-induced cell death, mRNA expression of the two receptors were quantified by real time PCR.Treatment of LNCaP cells with cannabis extract

Effect of Cannabis Extract on Androgen Receptor and PSA in LNCaPCells
Androgens are involved in the development and progression of prostate cancer where androgen receptor is assumed to be the essential mediator for androgen action.In the next series of experiments, we determined the effect of cannabis extract on mRNA expression of androgen receptor.In a dose-dependent study, we found that treatment of LNCaP cells with cannabis extract resulted in a marked decrease in androgen receptor expression (Figure 5(a)).Studies have also shown that modulation in androgen receptor leads to alteration in androgen-responsive genes [31].PSA is an androgen-responsive gene and is regarded as the most sensitive biomarker and screening tool for prostate cancer in humans [32].The dose-dependent effect of cannabis extract on LNCaP cells showed a significant decrease in PSA mRNA expression at 20, 40, 60 and 70 µg/ml CBD concentration when assessing at 24 hours post-treatment (Figure 5(c)).We next examined the effect of cannabis extract on secreted levels of PSA in LNCaP cells.Employing ELISA technique, we found that treatment of LNCaP cells with cannabis extract resulted in a dose-dependent decrease in the secreted levels of PSA by 57.9%, 49%, 33.5%, 24.9% and 17.1% at 20, 40, 60 and 70 µg/ml CBD concentrations, respectively (Figure 5(d)).From these data, it seems that the decrease in LNCaP cell growth was concomitant with a decrease in androgen receptor mRNA expression as well as a decrease in secreted PSA level.

Effect of Cannabidiol on VEGF
Because VEGF is a marker for angiogenesis, blocking the angiogenic process may represent a promising way of  treating the tumor.Studies have shown that androgen regulates VEGF content in prostate cancer [33].As cannabis extract treatment resulted in a decrease in androgen receptor expression, the effects on VEGF were also determined.It was observed that cannabis extract treatment resulted in a dose dependent decrease in VEGF mRNA expression (Figure 5(b)).

Effect of Cannabis Extract on Apoptosis of LNCaP Cells
We next assessed whether the cell growth inhibitory effect of cannabis extract was associated with induction of apoptosis.We quantified the extent of apoptosis by flowcytometric analysis of the cells labeled with Propidium iodide (PI).LNCaP cells were treated with cannabis extract (20 -60 µg/ml CBD, 10 -30 µg/ml THC and 0.4 -12 µg/ml CBN) for 24 hours.As shown by the data in Figure 6(a), cannabis extract treatment of LNCaP cells resulted in 10.1%, 25% and 42% of apoptotic cells at a dose of 20, 40 and 60 µg/ml CBD respectively.Whereas the induction of apoptosis was almost negligible at the lowest dose (20 µg/ml CBD) used, the highest dose employed (60 µg/ml CBD) resulted in a massive induction of apoptosis.Cannabis extract treatment of PC3 cells resulted in 21%, 21% and 39.4% of apoptotic cells at a dose of 20, 40 and 60 µg/ml CBD respectively (Figure 6(b)).Both LNCaP and PC3 showed G0/G1 cell cycle arrest of cells at 40 and 60 µg/ml CBD treatment.

Anti-Inflammatory Properties of High CBD Cannabis Extract on Dermal Fibroblasts
Prior to assessing anti-inflammatory properties of the cannabis extract, the ability of LPS to induce secretion of key cytokine-related factors (IL-6 and IL-8,) was assessed in dermal fibroblast cultures.After 24 h of LPS  .As an assessment of potential anti-inflammatory properties of cannabis extract, we observed that accumulation of the indicated cytokines in conditioned media of the LNCaP cells was significantly suppressed by 20 µg/ml CBD cannabis extract treatment.MTS assays performed on LNCaP cells at the end of the experiment indicated that none of the LPS or cannabis extract treatment combinations affected cell viability relative to vehicle controls (data not shown).Additional control tests showed that the cannabis extract did not interfere significantly in any of the ELISA steps.

Cannabidiol Inhibits Prostasphere Formation of LNCaP Cells under Non-Adherent Culture Conditions
The ability to form prostaspheres in non-adherent culture is one of the characteristics of prostate CSCs (Reynolds et al. 1992) [34].To confirm that cannabis extract treatment can inhibit prostate CSC properties, prostasphere formation of LNCaP cells was studied in the presence or absence of cannabis extract.500 hundred LNCaP

Discussion
Prostate cancer is the most frequently diagnosed cancer in western males [35].The progression of most of the cancers including prostate cancer may be a result of defects in cell cycle and apoptotic machinery.Thus, the agents which can modulate apoptosis in cancer cells may be useful in the management and therapy of cancer.Hence, there is a need to develop novel targets and mechanism-based agents for the management of prostate cancer.One of the most exciting and promising areas of current cannabinoid research is the ability of these compounds to control the cell survival/death decision [7].In this study, we found that compared with PrEC cells, the expression levels of both cannabinoid receptors CB1 and CB2 were significantly higher in human prostate cells LNCaP, DU145, PC3 (Figure 2).These data suggest that CB1 and CB2 receptors could be targets for treatment options for prostate cancer.We also found that cannabis extract treatment of LNCaP and PC3 cells resulted in a decrease of cell viability as determined by MTS assay at varying doses (Figure 8(c) & Figure 8(d)), suggesting the involvement of both CB1 and CB2 in the anti-proliferative action of cannabinoids.Treatment of LNCaP cells with varying doses of cannabis extract resulted in down regulation of mRNA expression of CB1 and CB2 implicating cytopathic effect of cannabis extract on PCa cells (Figure 4).Apoptosis is a physiological and discrete way of cell death and is regarded to be an ideal way of cell elimination.In this study, we also observed an increase in apoptosis of LNCaP cells by treatment with cannabis extract and this was confirmed by flow cytometry (Figure 6 Androgens are essential for the growth, differentiation, and functioning of the prostate as well as in increasing prostate cancer development [36].The over expression of androgen receptor in prostate cancer may promote cell growth.Hence, elimination or reducing the androgen receptor in prostate cancer should help in treating this neoplastic disease.We further studied the effect of cannabis extract on androgen receptor mRNA expression and its subsequent effect on PSA production.Our results indicate that cannabis extract treatment significantly decreases androgen receptor mRNA expression (Figure 5(a)) in LNCaP cells.PSA is an androgen receptor (AR)-regulated serine protease produced by prostate epithelial cells [37], and is the most widely employed marker in the detection of early prostate cancer.Therefore, agents which could reduce PSA levels may have important clinical implications for prostate cancer.Earlier studies reported that PSA is primarily regulated by androgens [38].Increased PSA level is used extensively as a biomarker of prostate diseases including prostatitis, benign prostatic hypertrophy, and prostate cancer.It is reported that in LNCaP cells, androgens regulate PSA glycoprotein expression and mRNA via androgen receptor [39].Our studies show a significant decrease in intracellular mRNA (Figure 5(c)) as well as secreted levels of PSA (Figure 5(d)) by cannabis extract treatment of cells, suggesting that cannabinoid receptor agonists may be exploited to prevent prostate cancer progression.VEGF is a ubiquitous cytokine that regulates embryonic vasculogenesis and angiogenesis.Normal prostate epithelium expresses low levels of VEGF, whereas increased levels are reported in prostate carcinoma [40].Studies have shown that cannabinoid treatment markedly reduced the expression of VEGF in gliomas, the most potent proangiogenic factor and also of angiopoietin 2, which contributes to the angiogenic process by preventing vessel maturation [41].Our results showed that treatment of LNCaP with cannabis extract inhibits VEGF mRNA expression in LNCaP cells (Figure 5

(b)).
Chronic inflammation has been linked to various steps in tumor formation, including cellular transformation, proliferation, invasion, angiogenesis, and metastasis [42].Among the inflammatory cytokines, interleukin 1 (IL-1), IL-6, IL-8, and IL-10 have been reported as present in the prostate cancer cells [43], indicating the significance of these inflammatory factors in prostate cancer progression.Therefore, controlling inappropriate inflammation would appear to be one strategy that might help control cancer progression.Prior to assessing anti-inflammatory properties of cannabis extract, the ability of LPS to induce secretion of key cytokine-related factors (IL-6 and IL-8) was assessed in dermal fibroblasts.After 24 h of LPS stimulation, dermal fibroblast conditioned media contained substantially elevated levels of cytokine IL-6 and chemokine IL-8 (Figure 7(a) & Figure 7(b)).Our results show that accumulation of the indicated cytokines in conditioned media of dermal fibroblasts was significantly suppressed by cannabis extract treatment confirming potential anti-inflammatory property of the high CBD cannabis extract preparation that may be considered to target chronic inflammation in prostate cancer.
Prostate cancer cells are in general highly resistant to common chemotherapeutic agents and the presence of CSCs is suggested to contribute to chemo resistance.The ability to form spheres in non-adherent, serum free conditions is a key property of stem cells (Reynolds et al. 1992) [34].In this study, prostate cancer cell line LNCaP was able to form spheroids in non-adherent culture, suggesting the presence of cancer stem-like cells within these cell lines.Because prostaspheres are enriched with CSCs [44], the inhibitory effect of cannabis ex-

Figure 1 .
Figure 1.Example of chromatogram and retention times of the compounds (CBD, THC and CBN) (a) Standard, (b) and (c) Herbal cannabis samples.
(a) & Figure 3(b) show that treatment of PrEC cells with cannabis extract (20 -80 µg/ml CBD) for 24 hours had no effect on cell viability.However, treatment of LNCaP and PC3 cells with similar doses of cannabis extract in a dose-dependent manner significantly decreased the viability at 24 hours (Figure 3(c) & Figure 3(d)).

Figure 2 .
Figure 2. CB1 & CB2 Expression are increased in Prostate cancer cells.Expression of CB1 and CB2 in human prostate epithelial cells and prostate cancer cells.mRNA expression of CB1 and CB2 was determined in androgen dependent LNCaP with and without R1881, androgen independent DU145 and PC3 prostate cancer cell lines.Expression of CB1 and CB2 mRNA was up regulated in PCa cell lines as compared to BPH1 and PNT1B prostate epithelial cell lines.

Figure 3 .
Figure 3. Cannabinoids are selectively cytotoxic to PCa.Effect of cannabis extract on cell viability: A, and B, effects on the viability of PrECs A & B, LNCaP (c) and PC3 (d).As detailed in Materials and Methods, the cells were treated with cannabis extract in different doses for 24 hours, and their viability was determined by MTS assay.Columns, means; bars, ± SE of three separate experiments; * P < 0.001 compared with control (0 CBD).

Figure 4 .Figure 5 .
Figure 4. Evidence for cytopathic effects of cannabinoids on PCa cell lines.Effect of cannabis extract on mRNA expression of cannabinoid receptors CB1 and CB2 in human prostate epithelial cells and prostate cancer cells.Dose dependent effect of cannabis extract on mRNA expression of CB1 and CB2 was determined in LNCaP cells by quantitative real time-PCR from representative experiments repeated thrice with similar results.Relative levels of expression normalized to the mRNA level of 18 Srna.The fold change in LNCaP cells were compared with PrEC.

Figure 6 .
Figure 6.Quantification of apoptosis by Flow Cytometry.Effect of cannabis extract on apoptosis in LNCaP cells.(a) and (b) Quantification of apoptosis in LNCaP and PC3 cells by flow cytometry.The cells were treated with cannabis extract (20 µg/ml of CBD) for 24 hours.The cells were trypsinized, washed with PBS and fixed with ice cold 70% ethanol.Cells were subsequently stained with a propidium iodide (PI) for 1 hour.Samples were analyzed by flow cytometer and BD FACS Diva Software and apoptosis rate in PCa cell lines were calculated as the percentage of cells in sub G0/G1 and Go/G1 phase.Data from representative experiments repeated thrice with similar results.stimulation, dermal fibroblast conditioned media contained substantially elevated levels of IL-6 and IL-8 (Figure 7(a) & Figure 7(b)).As an assessment of potential anti-inflammatory properties of cannabis extract, we observed that accumulation of the indicated cytokines in conditioned media of the LNCaP cells was significantly suppressed by 20 µg/ml CBD cannabis extract treatment.MTS assays performed on LNCaP cells at the end of the experiment indicated that none of the LPS or cannabis extract treatment combinations affected cell viability relative to vehicle controls (data not shown).Additional control tests showed that the cannabis extract did not interfere significantly in any of the ELISA steps.

Figure 7 .
Figure7.Cytokines/chemokine: Dermal fibroblasts Treatment with Cannabis extract 24 h.Suppression of lipopolysaccharide (LPS)-stimulated accumulation of sentinel cytokines/chemokines by cannabis extract in dermal fibroblasts.Dermal fibroblasts were grown in 12 well plates (Corning) to produce confluent monolayers in DMEM with 5% fetal bovine serum.LPS was added to the culture media at 1 μg/ml for 24 h ± 45 or 20 μg/ml CBD cannabis extract.Accumulation of IL-6 (a) and IL-8 (b), was determined from conditioned media by enzyme-linked immunosorbent assay.Cytokine/chemokine accumulation was compared with levels of LPS-stimulated samples, expressed as pg/ml, * p < 0.001.cells were seeded onto non-adherent 6-well plates and treated with either cannabis extract or vehicle for 14 days.Images of the prostaspheres were captured under the microscope.The number of prostaspheres formed was counted at the end of 14 days.The cannabis extract treatment (20 µg/ml CBD) efficiently suppressed the spheroid formation ability of LNCaP cells.LNCaP cells spheroids in the vehicle measured 150 µm to 250 µm diameter after 14 days.The cells treated with cannabis extract did not form spheroid and appeared as single or group of 2 or 4 cells after 14 days (Figure8(a) & Figure 8(b)).
(a) & Figure 6(b)).The observation could be useful for the management of human prostate cancer.

Figure 8 .
Figure 8.Effect of cannabis extract on LNCaP prostaspheres formation: Spheroid formation assay was performed with cells treated with cannabis extract or vehicle.500 LNCaP cells were seeded onto non-adherent 6-well plates and treated with either cannabis extract or vehicle for 14 days.The number of prostaspheres formed was counted at the end of 14 days.(a) Typical LNCaP prostaspheres generated after 14 days and image of the prostaspheres was captured under microscope, 10× magnification.LNCaP prostaspheres in the vehicle measured 150 µm to 264 µm diameter after 14 days.(b) Note that prostaspheres are reduced to single or group of 4 cells in the cannabis extract treated group.The cannabis extract treatment efficiently suppresses the spheroid formation ability of LNCaP cells.