Phytoecdysteroids from Silene praemixta

The waste products of the production of ecdysterone (3) from Silene praemixta M. Pop. (Caryophyllaceae) were investigated. The column chromatography of the technological waste has allowed to isolating 2-desoxyecdysone (1), 2-deoxyecdysterone (2), ecdysterone (3) and 22-O-β-D-glucopyranoside of 2-deoxyecdysone (4). Glucoside 4 was found for the first time in the plant Silene praemixta. Ecdysteroids 1, 2 prevailed in the sum. Compounds 3 and 4 were minor components of the waste products. The identification of compounds 1-4 was carried out by using the modern methods of 1D and 2D NMR spectroscopy: H, C, DEPT, H-H COSY, HSQC, NOESY, IR spectroscopy and ESI MS.

The genus of Silene (Caryophyllaceae) is widely distributed in Uzbekistan and it is known as the rich source of phytoecdysteroids.The one main of them is the ecdysterone, which is possessed as an anabolic effect for human body [2].The ecdysterone has the ability to adapt organism to experimental effects of environment and stimulate the operability [3].
The "Ecdisten" preparation is produced from the medicinal plants Raponticum and Silene at the Institute of the Chemistry of Plant Substances (Tashkent, Uzbekistan Academy of Sciences).

RESULTS
In the present paper the chemical composition of Silene praemixta plant was investigated.We described the isolation and structure elucidation of ecdysteroids from the waste product of this manufacture.

DICUSSION
Using infrared spectrophotometer data we obtained information regarding presence of hydroxyl groups (3387 -3409 sm −1 ) and 6-ketochromaphore (1639 -1652 sm −1 ) of tested compounds according to ecdysteroids skeleton.The mail role in the structure determination of isolated compounds plays NMR analysis which shows presence of signals of methyl groups at 0.

EXPERIMENTAL
General Methods 1 H, 13 C NMR (Table 1) spectra were run in CD 3 OD, C 5 D 5 N using TMS as internal reference on a Bruker Avance DRX 600 MHz spectrometer.ESI-MS spectra were recorded on a PE Q-STAR electrospray, ionization time of flight-tandem-mass spectrometry spectrometer.Silica gel 60 PF 254 was used for TLC.Infrared spectra were taken on Perkin Elmer Spectrum 100 FTIR sprectrophotometer, which are conformed with literature data.
Chemical shifts with the whole numbers are not indicated in spectra.Chemical shifts for absent signals are not found.Signals with the same characters cover mutually inside of the column.