Purification , Crystallization and Preliminary X-Ray Diffraction Analysis of Exodeoxyribonuclease III from Crenarchaeon Sulfolobus tokodaii Strain 7

Exodeoxyribonuclease III (EXOIII) acts as a 3’→5’ exonuclease and is homologous to purinic/apyrimidinic (AP) endonuclease (APE), which plays an important role in the base excision repair pathway. To structurally investigate the reaction and substrate recognition mechanisms of EXOIII, a crystallographic study of EXOIII from Sulfolobus tokodaii strain 7 was carried out. The purified enzyme was crystallized by using the hanging-drop vapor-diffusion method. The crystals belonged to space group C2, with unit-cell parameters a = 154.2, b = 47.7, c = 92.4 Å, β = 125.8 ̊ and diffracted to 1.5 Å resolution.


Introduction
A variety of mechanisms exist to repair damaged DNA and maintain a high degree of genomic stability within cells.Endonucleases specific for abasic or apurinic/ apyrimidinic (AP) sites are a major component of the cellular DNA repair machinery.AP sites are generated by spontaneous base loss [1], direct action of reactive oxygen species [2] and damage-specific DNA glycosylases as repair intermediates in the DNA base excision repair (BER) pathway [3,4].It is estimated that as many as 10,000 purine bases alone are lost in a mammalian cell each day [5].The generated AP sites are potentially mutagenic and cytotoxic, because of the inhibition of DNA replication and transcription [6,7].AP sites are specifically corrected by the BER pathway: AP endonucleases (APE) first recognize the AP site and then cleave the phosphodiester backbone immediately 5' of the AP site, leaving a free 3'-hydroxyl nucleotide end and a deoxyribose 5'-phospate as termini [8,9].
Exodeoxyribonuclease III (EXOIII; EC 3.1.11.2) is homologous to APE and acts as a 3'→5' exonuclease.It is considered that the AP site undergoes a unique con-formational change upon protein binding that permits complex formation and activation of attacking water, leading to incision, in the presence of Mg 2+ [10,11].However, the detailed reaction and substrate recognition mechanisms of AP endonucleases are not fully understood yet.Although EXOIII and APE belong to the same family, they have different substrate specificities.The three dimensional structures of EXOIII from Escherichia coli (E.coli) [12] and human APE [13] have been elucidated, but structures of these two enzymes from the same species have yet to be determined.To reveal the structural basis for the reaction and substrate recognition mechanisms of these enzymes from the same organism, we crystallized EXOIII and APE from the crenarchaeon, Sulfolobus tokodaii strain 7 [14].Sulfolobus tokodaii is an aerobic thermoacidophilic crenarchaeon that optimally grows at 353 K and at low pH.Therefore, we also hope to obtain structural insights into the thermostability of these enzymes by comparing their structures with those of corresponding enzymes from other mesophilic organisms.
Here, we report the expression, purification, crystallization and preliminary X-ray diffraction analysis of EX-OIII from Sulfolobus tokodaii strain 7, that is sto-EX-OIII.The enzyme consists of 241 amino acids (NCBI reference code NP_377894; UniProt ID F9VNP2), and has a molecular weight of 28.08 kDa and an isoelectric point of 8.72.

Materials and Methods
All chemicals were special grade reagents purchased from Wako Pure Chemical Industries, Japan unless stated otherwise.

Expression and Purification
The stoEXOIII protein was expressed in E. coli Rosettagami (DE3) using the pET-11a expression system (Merck KGaA, Germany).Since neither affinity tag nor extra residue was included, the sequence of the expressed protein was the same as that of the database entry.Bacterial clones were cultivated at 310 K in Luria-Bertani medium (Sigma-Aldrich, USA) with 100 μg•ml −1 ampicillin and 30 μg•ml −1 chloramphenicol.The cells were harvested by centrifugation at 8000 g, resuspended in 20 mM Tris-HCl pH 8.0, 50 mM NaCl and sonicated on ice.The mixture was heated at 343 K for 30 min and the soluble fraction was collected by centrifugation at 8000 g for 15 min.Ammonium sulfate was added to the supernatant to 50% saturation and the mixture was centrifuged at 10,000 g for 15 min.Ammonium sulfate was again added to the supernatant, to a final concentration of 60% saturation.After centrifugation at 10,000 g for 15 min, the pellet was dissolved in 50 mM sodium phosphate buffer (pH = 7.0) and dialyzed against the same buffer.The desalted mixture was loaded onto a SP-Sepharose column (1 ml; GE Healthcare Life Sciences) pre-equilibrated with 50 mM sodium phosphate buffer (pH = 7.0).The protein was eluted with a linear gradient of 0 -1 M NaCl in the same buffer and protein purity was confirmed by SDS-PAGE.Simultaneous removal of NaCl and concentration of the protein was achieved by several rounds of diafiltration using an Amicon Ultra-10 Centrifugal Filter device (Millipore) with 20 mM sodium phosphate buffer (pH = 7.0) to a final protein concentration of 10 mg•ml −1 .The final yield of pure protein was around 0.9 mg per liter of culture.

Crystallization
Initial crystallization conditions were screened by the hanging-drop vapour-diffusion technique at 293 K using Index, Crystal Screen and Crystal Screen 2 crystal screening kits (Hampton Research, USA).The crystallization droplets were prepared on siliconized cover slides by mixing 1 μl protein solution with 1 μl reservoir solution; the drops were then equilibrated against 800 μl of reservoir solution.Several single crystals appeared within a few days in several solutions containing PEG 4000 -8000 (1st grade, Wako, Japan) as precipitant.Crystallization conditions were further optimized manually by refinement of buffer choice and PEG concentration.The quality of the crystals were checked from diffraction images obtained using an R-AXIS IV ++ X-ray diffractometer (Rigaku, Japan).Finally, the most suitable precipitant solution for crystallization was 15% (w/v) PEG 6000, 0.1 M Bis-Tris pH 5.5 or 0.1 M HEPES pH 7.0, and 0.1 M L(-)-proline.stoEXOIII protein crystals with approximate dimensions of 1.0 × 0.1 × 0.1 mm were obtained in about one week at 293 K (Figure 1).

Data Collection
A stoEXOIII crystal was picked from the crystallization droplet using a mounted nylon loop and placed directly into a cold nitrogen-gas stream at 100 K. X-ray diffraction data were collected with a CCD detector (Quantum 210, Area Detector Systems Co., USA) on beamline BL-NW12 of the Photon Factory-Advanced Ring (PF-AR), Tsukuba, Japan, using a wavelength of 1.00 Å.The distance between the crystal and detector was 110 mm.A total of 360 images with 0.5 degrees oscillation were collected with an exposure time of 10 seconds.

Results and Discussion
Diffraction data were obtained in the resolution range 100 -1.5 Å (Figure 2) and were then indexed and scaled using DENZO and SCALEPACK from the HKL-2000 program suite [15].The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 154.2,b = 47.7,c = 92.4Å, β = 125.8˚.A total of 87532 independent reflections obtained with a multiplicity of 5.1 at 1.5 Å resolution reached 99.3% completeness with an R merge of 6.7%.Assuming that two monomers are present in the asymmetric unit, the Matthews coefficient was calculated to be 2.4 Å 3 •Da −1 [16].This corresponds to an estimated   solvent content of 49%, which is in the range normally observed for protein crystals.The relevant data-collection statistics are summarized in Table 1.The crystal structure determination of stoEXOIII is under way by the molecular replacement method using the MOLREP program [17] in the CCP4 suite [18] at 1.5 Å resolution.

Conclusion
Exodeoxyribonuclease III from the crenarchaeon, Sulfolobus tokodaii strain 7, was purified and then crystallized by using the hanging-drop vapor-diffusion method.

Figure 2 .
Figure 2. X-ray diffraction image.A corner section at the upper left is enlarged and shown in the inset.

Table 1 . Data-collection statistics. Values in parentheses are for the highest resolution shell.
is the intensity of the ith observation of reflection hkl and I hkl is the average intensity of reflection hkl.