5-Aminolevulinic Acid ( 5-ALA ) : Analysis of Preclinical and Safety Literature

Problem: 5-ALA has been used for many years at relatively high dose amounts in single doses for photodynamic therapy and immunofluorescence of tumors. An analysis of compiled data relating to safety and any side-effects about the use of 5-ALA at low doses has not yet been published. Purpose: This report analyzes data about the safety of the use of 5-Aminolevulinic Acid (5-ALA) in low doses as a supplement over an extended period of time. Methods: This investigation is a systematic analysis of the current literature ((Medline, and SBI) and snowballing techniques) related to the safety and efficacy of 5-ALA in animals and humans. Clinical trials in progress using 5-ALA were also analyzed. Constant comparative analyses were used to synthesize the findings. Results: The safety of low-dose 5-ALA as a supplement has been demonstrated by animal and human studies. The results suggest that none of the investigations document the presence of symptoms or abnormal laboratory results of clinical significance. The minor laboratory changes documented were judged not clinically significant.


Introduction
Nucleotides → Genetic Information; Amino Acids → Structure & Metabolism; Previously, 5-ALA has been used for many years at relatively high dose (1000 to 2000 mg/day) in single doses for photodynamic therapy and immunofluorescence of tumors.5-ALA at low doses (between 2.5 to 50 mg/day) is currently being used as a dietary supplement in Japan.An analysis of compiled data relating to safety and any sideeffects about of the use of 5-ALA at low doses has not yet been published.It is a natural non-alpha amino acid.5-ALA is a delta amino acid and is not a component of protein.As seen in Table 1, it is found in many common foods and fermented products such as spinach, tomatoes, wine, sake, etc. 5-ALA → Energy Conversion.

5-ALA Facts
The blood concentration of 5-ALA in normal human beings is approximately 50 μg/L.The typical intake from food is approximately 1 -2 mg/day.Five-ALA is synthesized by the body at a rate of 600 mg/day [1].About 1 mg of 5-ALA is in plasma [2].Normal plasma level in 5-ALA was 92 nmol/l (SD = 39, n = 89 with a range of 24 -270 nmol/l) [2].
The internal heme pool has about 60 g in hemoglobin and 8 g in other heme enzymes.Five-ALA is excreted in the urine and as bilirubin at a rate of 2 mg/day.The bioavailability is 100% (D i.v /D p.o ).The t max in plasma is 0.76 h and the t 1/2 in plasma is 0.92 h as estimated from a sample of healthy human (18 -55 y/o) males, N = 12, given 20 mg/kg of 5-ALA [2].5-ALA is synthesized in the mitochondria.It is a building block of protoporphyrin and a precursor of both chlorophyll and heme.The safety of 5-ALA combined with iron in a supplement at low doses has been demonstrated by animal and human studies.5-ALA has been associated with the origin of life.There are three fundamental components: The oral 5-ALA product used in all of the safety studies were produced by Cosmo Oil Co. Ltd.The supplement Until recently 5-ALA was difficult and costly to produce.Using 5-ALA to treat cancer was a preferred alternative to ionizing radiation therapy due to the risks associated with radiation exposure.contains 3 components: 1) 5-Aminolevulinic Acid (5-ALA) phosphate salt; 2) Citric iron; 3) Corn starch (as a filler).
It is a Non-Genetically Modified Organism (GMO), Bovine Spongiform Encephalopathy (BSE) free, alcohol free and the products are manufactured under food Good Laboratory Practice (GLP) conditions.A certificate of analysis is available.No heavy metals in the analyses (Pb, As, Hg, Cd) were detected and a microbial analysis revealed viable bacterial counts < 300/g.Neither E. coli or S. aureus were detected [3,4].

Studies Completed
Preclinical and Safety Tests suggest that 5-ALA is safe at modest doses.A summary of these findings from investigations conducted by Cosmo Oil & SBI can be seen in Tables 2 and 4 [7,8].In these studies, 3 different doses of 5-Aminolevulinic acid (5-ALA) phosphate, Sodium Ferrous Citrate (SFC) and Corn starch as filler were tested in humans.The steps were: 25 mg of 5-ALA/29 mg Sodium Ferrous Citrate (SFC) in step 1, 50 mg 5-ALA/58 mg SFC in step 2 and 75 mg 5-ALA/87 mg SFC in step 3 (See Table 2).Twenty-two participants (11 male and 11 female) in each step (total 66 subjects) took test food supplement for 4 weeks.Participants were healthy adults.This particular investigation used higher dosages than used in the 3 investigations that occurred in Hawaii.The investigations in Hawaii used a high dose of 50 mg/day.The safety of the test food supplement was evaluated by subjective symptoms and the clinical examination of subjects in the intake period.The change of laboratory test data were evaluated by using the one-sample t-test about the mean value of measurements obtained at 2 weeks and 4 weeks after administration, and 2 weeks after the end of the follow-up period, compared to those obtained just before the start of administration.
Cancer Treatment Photodynamic Therapy is a non-conventional light therapy for the treatment of cancer.Photodynamic therapy, also known as PDT, uses photosensitive drugs (5-ALA, Foscan, Metvix, Tookad, WST09, WST11, Photofrin and Visudyne) which are triggered by light from a specific wavelength, usually red or infrared, on the light spectrum chart [5].Depending on the type of cancer being treated, the medications are administered differently.A singlet oxygen molecule will form when the Adverse effects were noted in 19 subjects (6 subjects in step 1, 4 subjects in step 2, and 9 subjects in step 3).Total numbers of adverse events were 24 (6 events in step 1, 4 events in step 2, and 14 events in step 3).Symptoms in step 1 included diarrhea (2 events), headache (2), abdominal pain (1), facial swelling (1).Symptoms in step 2 included diarrhea (2 events), queasiness (1), stomach pain (1).Symptoms in step 3 included abdominal pain (4 events), rheumatoid symptoms (4), diarrhea (3), flatulence (2), stiff shoulders (1).All adverse events were selfrecognized subjective symptoms and judged by the Investigator to be unrelated to the study diet intake and to be mild in severity.Some data from laboratory test parameters showed statistically significant changes from baseline.All those changes (mild nausea, headache and GI upset) were relatively mild in severity and were judged by the Investigators to be clinically insignificant.[9].The Investigator is Hitoshi Suzuki, MD.There were 30 participants that consumed 97.4% of the additive.The food supplement additive was taken for 4 weeks with a 2 week follow-up.The investigators report no related symptoms and document some statistically significant laboratory changes, but not of clinical significance.The following summarizes these findings.Many parameters resolved over time and the clinical implications of these findings were judged to be without clinical consequence [3,10].
Another investigation conducted to study anemia involved 104 female subjects ages 20 -65 with Hemoglobin levels 8 -12 g/dl over a 12 week period.Daily doses of food supplementation were administered and expressed as 5-ALA phosphate/sodium ferrous citrate: 10.0 mg/92.0mg (high-dose); 5.0 mg/46.0mg (mid-dose); and 2.5 mg/23.0mg (low-dose) [11][12][13].This investigation also resulted in no safety problems of clinical significance.A study conducted at the University of Hawaii, John A. Burns School of Medicine (UHJABSOM): The Pre-Diabetes and Supplement Study involved 154 participants divided into three groups.Daily doses of 5-ALA phosphate/sodium ferrous citrate were: 15.0 mg/17.2mg (low-dose), 50 mg/57.4mg (high-dose) and control, over a 12 week period.In 2 hour post-Oral Glucose Tolerance Tests (OGTT), glucose levels declined significantly compared to those not taking the supplement (p = 0.02) [1].HbA1C results were of borderline significance (p = 0.07) and no untoward effects were reported [1].Another study conducted at the UHJABSOM was the Supplement Seep study.This study involved 40 participants divided into two groups a 50 mg intervention and a control, again no adverse events were reported.Lastly, an additional study was conducted at the UHJABSOM, the Supplement and Mood study.This study involved 40 participants divided into 2 groups, a 50 mg intervention group and a control group.There were no adverse events reported.Again, all of these studies had no safety problems of clinical significance [1,7,14].

Conclusion
None of the investigations document the presence of symptoms or abnormal laboratory results of clinical significance.Minor laboratory changes were judged to be not clinically significant.For the study conducted in Hawaii, diarrhea and abdominal pain were incorporated into the informed consent for the Supplement Sleep and Mood Studies and the protocol was modified to add laboratory assessments at 4 and 8 weeks into the study.The University of Hawaii Institutional Review Board approved both investigations.No clinically significant abnormalities were observed in these studies that could be attributed to the food supplement.