Soil and Variety Effects on the Energy and Carbon Balances of Switchgrass-Derived Ethanol

This study examined the effects of soil and switchgrass variety on sustainability and eco-friendliness of switchgrassbased ethanol production. Using the Agricultural Land Management Alternatives with Numerical Assessment Criteria (ALMANAC) model, switchgrass biomass yields were simulated for several scenarios of soils and varieties. The yields were fed to the Integrated Biomass Supply Analysis and Logistics (IBSAL) model to compute energy use and carbon emissions in the biomass supply chain, which then were used to compute Net Energy Value (NEV) and Carbon Credit Balance (CCB), the indicators of sustainability and eco-friendliness, respectively. The results showed that the values of these indicators increased in the direction of heavier to lighter soils and on the order of north-upland, south-upland, north-lowland, and south-lowland varieties. The values of NEV and CCB increased in the direction of dry to wet year. Gaps among the varieties were smaller in a dry year than in a wet year. From south to north, NEV and CCB decreased for lowland varieties but increased for upland ones. Thus, the differences among the varieties decreased in the direction of lower to higher latitudes. The study demonstrated that the sustainability and eco-friendliness of switchgrass-based ethanol production could be increased with alternative soil and variety options.


Introduction
Ethanol is a potential alternative energy source due to its economic, environmental, societal, and strategic benefits.Ethanol can be produced from three kinds of plant materials: lignocellulose, starch, and sugar.Sugar-and starchbased approaches have challenges related to food and feed security, grain price increase, and environmental degradation [1].
Lignocellulosic feedstocks have the potential to be the most promising future feedstock source [2].However, lignocellulosic ethanol production technology is still evolving.Lignocellulosic ethanol can be produced from crop residues, woody species, and herbaceous crops such as switchgrass (Panicum virgatum L.).
Switchgrass is an economically, energetically, and ecologically viable dedicated energy crop [3].It has high biomass productivity, high input use efficiency, broad geographic adaptability, low environmental risk, and low production cost [4][5][6][7].Switchgrass is a C4 species using the photosynthetic pathways that have higher photosynthetic, water, and nitrogen use efficiencies and greater tolerance to heat, nitrogen, and water stresses [8,9].These physiological attributes lead to high biomass pro-ductivity in switchgrass, especially under water-and nutrient-limited conditions.Furthermore, the high leaf area index and low light extinction coefficient of switchgrass contribute to high radiation use efficiency and thus to higher growth rate and productivity [9,10].High productivity is also due to soil improvement through enhanced carbon sequestration by its extensive and deep root system.High carbon sequestration belowground also leads to low carbon emissions from switchgrass production [6].Large, deep root system also helps enhance water and nutrient use efficiencies by acquiring the resources from deeper soil layers.Switchgrass has advantage over some crop residues in its smaller ash and larger energy contents [11].It is tall, tough, and resistant to drought, flooding, and many pests and diseases [8].
Switchgrass is an allogamous species, resulting in highly heterogeneous and variable populations.North American switchgrass populations fall in two different cytotypic groups: lowland and upland [12].Within a cytotype, the switchgrass populations have further been classified into two groups based on the latitude of origin: south and north [13].The cytotypic differentiation and latitudinal (ecotypic) adaptation in switchgrass lead to four varietal groupings: north-lowland, north-upland, south-lowland, and south-upland [14].The productivity of switchgrass strongly depends on the cytotype and ecotype of varieties [7] and on location and soil [15].The inherent variability in switchgrass productivity due to variations in soil and variety could affect the sustainability (in terms of nonrenewable energy replacement) and the eco-friendliness (in terms of carbon emissions reduction) of switchgrassbased ethanol production.Previous studies that examined the sustainability and eco-friendliness of ethanol production systems did not explicitly address the effects of variability in soil and variety on energy crop yields and the associated ethanol production [16].Because switchgrass productivity is influenced by the day-length sensitivity of switchgrass phenology [17], the effect of latitude on the sustainability and eco-friendliness of ethanol production needs to be studied.
Due to long growing seasons and high rainfall, the southeastern United States is more suitable for biomass production than southwestern or northern region of the country.In Mississippi, a southeastern state, favorable weather and soil conditions make switchgrass a viable option for farmers.The response of switchgrass to variability in climate, soil, and variety is largely unknown for the southeastern United States [18].Detailed knowledge about the impacts of this variability on switchgrass productivity and the sustainability and eco-friendliness of switchgrass-based ethanol production might be helpful in evaluating this grass as a potential future energy feedstock.Thus, more information is needed to characterize the productivity, sustainability, and eco-friendliness of switchgrass as a bioenergy crop in relation to soil and variety in this region.
The production and use of biofuels is associated with the issue of long-term energy security, that is, energy sustainability [19].The sustainability issue is important because energy security is currently associated with importing fuel from other countries.The sustainability of biofuel production in terms of non-renewable energy replaced may be quantified using a measure, called the Net Energy Value (NEV), which is defined as renewable energy produced minus non-renewable energy used to produce the renewable energy.A larger NEV value indicates that more non-renewable energy is replaced, and a positive value signifies that ethanol production is sustainable in terms of energy security, that is, as a non-renewable energy replacement.
The emission of CO 2 , a greenhouse gas, during the delivery of feedstock and ethanol processing is another important issue [20].Increasing CO 2 emissions have been identified as a cause of global climate change, which in turn is a driver of environmental problems.As concern about global climate change grows, studying CO 2 emissions becomes increasingly important.Many studies have used CO 2 emissions as a basis for determining the effect of ethanol production on the environment.With an increase in plant biomass yield, the amount of carbon emitted during delivery and processing also increases due to the increased use of fossil fuel.Although yield increase is generally beneficial, the associated increase in emitted carbon is not.The current approach, which evaluates the harmful effects of an ethanol production system in terms of the amount of CO 2 emitted to the atmosphere, gives an impression that increasing plant biomass yield is not environmentally beneficial, which is not always true.To reflect the positive aspect of increased yield as well as the negative aspect of the associated increased emissions, a new approach is followed in this study-using Carbon Credits Balance (CCB).One carbon credit denotes a reward for extracting 1 Mg of CO 2 gas from the atmosphere.The CCB is defined as Carbon Credits Earned (CCE) minus Carbon Credits Used (CCU), where CCE is the amount of CO 2 (Mg) taken by the switchgrass plant from the atmosphere through fixation and stored in the harvested biomass, and CCU is the amount of CO 2 (Mg) emitted to the atmosphere while producing and supplying a given quantity of switchgrass biomass to a refinery, processing the ethanol at the refinery, and transporting, distributing, and combusting the ethanol after its production.A positive (negative) CCB value indicates that the ethanol production system is (not) environmentally friendly in terms of carbon emissions reduction, and a larger (smaller) value indicates that the system is more (less) environmentally friendly.
This study examined how variations in soil and variety would affect the sustainability (in terms of non-renewable energy replacement) and eco-friendliness (in terms of carbon emissions reduction) of switchgrass-based ethanol production in Mississippi.Specifically, the study explored the soil and variety effects on the NEV and CCB of ethanol derived from switchgrass.

Materials and Methods
This is a simulation study.Switchgrass biomass yields were simulated using the Agricultural Land Management Alternatives with Numerical Assessment Criteria (AL-MANAC) [21], a widely used process-based plant model that realistically simulates switchgrass biomass yields for a wide range of environments, including those in the southeastern United States [18,22].The ALMANACsimulated biomass yield was used as an input to Integrated Biomass Supply Analysis and Logistics (IBSAL) model [20] to estimate the energy use and CO 2 emissions associated with supplying feedstock from a production field to a biorefinery facility.The energy use and carbon emissions from IBSAL simulations were later used to compute NEV and CCB.

Models-A Brief Introduction
The ALMANAC is a process-based plant model designed to quantify key plant-environment interactions influenceing productivity and resource use [21].The major processes simulated are light interception, dry matter production, biomass accumulation, biomass partitioning, water and nutrient uptake, and growth constraints.The model simulates plant growth for each of a wide range of species using soil water and plant nutrient balances, Leaf Area Index (LAI), light interception, and radiation use efficiency [22].The stresses of heat, water, and nutrient reduce LAI and biomass growth.Light interception by the leaf canopy is estimated using the Beer's law and LAI, and the LAI is computed using a sigmoid-curve.Plant development is temperature driven, with growth stage duration depending on degree days [23].The inputs required are weather (maximum and minimum air temperatures, precipitation, and incident solar radiation), soil, tillage, and crop parameters.
The IBSAL is a dynamic simulation model for estimating delivered cost, energy use, and carbon emissions associated with various logistic operations in a biomass feedstock supply chain [20,24].The modeling platform consists of a network of several operational modules and connectors.A module represents an event, such as harvesting, swathing, baling, stacking, collection, preprocessing, storing, or transportation, or a process, such as drying, wetting, or carbohydrate breakdown.Weather (air temperature, relative humidity, wind speed, precipitation, and snow fall), biomass yield, crop harvest progress, equipment parameters, and machinery costs are the main inputs of the model, whereas delivered cost, delivered biomass, dry matter loss, energy consumption, and carbon emissions are key outputs.The output computations common to all operations are gathered into individual modules, which are independently constructed as a black box with a set of inputs and outputs.A complete model simulates the flow of materials from a biomass production field to a refinery.The model can also estimate the equipment, labor and time required to finish an operation [20].The model includes several energy crops and crop and forest residues.

Sites and Data
Three locations in Mississippi were selected based on weather data availability, potential growing area, and latitude: Meridian (32.33˚N, 88.75˚W), Grenada (33.77˚N, 89.82˚W), and Tunica (34.68˚N, 90.42˚W), which lie in the Central Prairies, North Central Hills, and Delta regions of Mississippi state, respectively.The Central Prairies, one of the most fertile farming regions of the state, comprises wide rolling grasslands that are easily converted to farmland.The North Central Hills consists of ridges and valleys with mostly alfisol soils.The Delta region, an alluvial plain in the northwest section of the state, is remarkably flat and contains highly fertile soils.This region is a major agricultural area in the state, and agriculture is the mainstay of the economy.
For each location, daily weather data of the climatic normal period of 1971-2000 were obtained from the Delta Agricultural Weather Center (www.deltaweather.msstate.edu)and the National Climate Data Center (www.ncdc.noaa.gov/oa/climate/stationlocator.html).These data comprised maximum and minimum air temperatures and precipitation only.The daily values of solar radiation for these locations and years were estimated using the WP method described by Woli and Paz [25].

Factors and Treatments
Based on the suitability to growing switchgrass and the amount of area of each soil, the soils considered for Tunica were silt loam, sandy loam, silty clay loam, silty clay, and clay.For Grenada, these were silt loam, silt, sand, silty clay loam, and clay.For Meridian, the soils were loam, loamy sand, sand, and sandy loam.The necessary data about the soils were obtained from the Natural Resource Conservation Service (soils.usda.gov/survey/printed_surveys/state.asp?state=Mississippi&abbr= MS; soildatamart.nrcs.usda.gov).The switchgrass varieties considered were north-lowland, north-upland, southlowland, and south-upland.Rainfed farming was assumed because farmers do not generally irrigate switchgrass in this region.The effects of soil and variety on NEV and CCB were assessed for three weather conditions: dry, wet, and average.For the dry (wet) weather, the driest (wettest) year of the climatic normal period of 1971-2000 was chosen for each location.For an average weather, all the 30 years were considered.

Biomass Yield Simulation
For these analyses, the ALMANAC-simulated switchgrass biomass yields were used.Because the model has been applied successfully in many locations, most of its parameters were not changed.The default parameter values used in several studies have given fairly reasonable results [6,22].In this study, therefore, only Radiation Use Efficiency (RUE), Maximum Leaf Area index (DMLA), light Extinction coefficient (EXTINC), and Potential Heat Unit (PHU) parameters were adjusted based on literature and expert knowledge.Values used for RUE were 4.7 g•MJ -1 for south-lowland [18], 78% of south-lowland for north-upland and south-upland, and 94% of southlowland for north-lowland.The EXTINC was set at 0.33, and the following values were used for DMLA [6]: 6 for south-lowland and north-lowland, 5 for south-upland, and 3 for north-upland.The PHU values used for simulations were 2300 for south-lowland, 2200 for north-lowland, 2150 for south-upland, and 2050 for north-upland.The values of DMLA, PHU, and RUE used for north-upland, south-lowland, and south-upland were based on expert knowledge (Jim Kiniry, personal communication).
Before application for yield simulations, ALMANAC was evaluated for Mississippi condition, for which 21 biomass yields belonging to Alamo (south-lowland), Kanlow (north-lowland), and Cave-in-Rock (north-upland) observed in Starkville, Mississippi during 2001-2007 were used.After the evaluation, switch grass biomass yields were simulated for 48 scenarios for Meridian (4 soils × 4 varieties × 3 years) and 60 scenarios for Grenada and Tunica each (5 soils × 4 varieties × 3 years).For simulations, planting date was assumed to be May 1, considering mid-April to mid-June as the planting window for Mississippi.Fertilizers were given as recommended by the Mississippi Extension Service.Considering less energy and fertilizer requirement, higher feedstock quality, more nutrient translocation and carbon sequestration, and less greenhouse gas emissions, once-a-year harvesting approach was used [19].

Biomass Logistics
Using the ALMANAC-simulated biomass yield as an input to IBSAL, the energy use and CO 2 emissions associated with supplying the feedstock from the production field to a biorefinery facility were estimated for each of the 48 or 60 scenarios belonging to each location.The harvest window for IBSAL use was assumed to start in the beginning of October [26] and continue until the end of December as harvesting until this time and over this period can reduce the amount of nutrient uptake and also result in maximum biomass yield [27].During the harvest window, the area of switchgrass harvested was assumed to be uniformly distributed.The moisture content of standing switchgrass at harvest was assumed to be 25% in early October [28], decrease linearly to 15% at the end of November and remain the same thereafter [27].The demand for switchgrass feedstock was assumed to be 2000 Mg•d -1 [27,29].

NEV Computation
The NEV of switchgrass-based ethanol production was computed as renewable energy obtained from the switchgrass-derived ethanol minus non-renewable energy used to obtain the renewable energy: where NEV is net energy value (MJ•ha -1 ); γ is switchgrass biomass to ethanol conversion ratio; Y is switchgrass biomass yield (Mg•ha -1 ); E e is the energy obtained from ethanol (MJ•L -1 ); and E f , E l , E p , and E t are the en-ergy used for biomass production, biomass logistics (harvesting, collection, storage, and transportation), ethanol processing (conversion), and ethanol transport and distribution, respectively, all in MJ•L -1 of ethanol produced.For γ, 334 L•Mg -1 was assumed [30].The value of Y for each scenario was simulated using ALMANAC.For E e , 21.2 MJ•L -1 was assumed [31][32].The E f was estimated from Y using the relationship given by Sokhansanj et al.
[29]: E f = (0.1036Y 2 -9.9909Y + 812.73)/γ.The E f thus estimated ranged from 1.9 to 2.2 MJ•L -1 , depending on Y as influenced by soil, variety, weather, and location.These E f values were about the same as those (1.5 -2.3 MJ•L -1 ) observed by Schmer et al. [31] and Qin et al. [33].The values of E l were estimated by dividing the IBSALcomputed energy use (MJ•Mg -1 ) by γ.For E p , 1.0 MJ•L -1 was used [32].For E t , 0.6 MJ•L -1 was estimated using GREET1_2011, the Greenhouse gases Regulated Emissions and Energy use in Transportation model [34].Switchgrass harvested in winter, especially after the first killing frost, removes less nutrients from the soil due to their retranslocation from foliage to crowns and roots [4,19].Switchgrass has an extensive and deep root system providing increased soil carbon storage.Thus, no significant depletion of nutrients or soil organic matter was assumed with the harvest, and no carbon or nutrient replacement cost was considered, accordingly.

CCB Computation
The CCB of a switchgrass-based ethanol production scenario was computed as the amount of CO 2 (Mg) fixed from the atmosphere by the harvested biomass minus the amount (Mg) released back into the atmosphere as: where CCB is the carbon credit balance (credits•ha -1 ); α is the carbon content of biomass (Mg•Mg -1 ); β is the CO 2 to C ratio (44/12 Mg•Mg -1 ); and C f , C l , C p , C t , and C c are the amounts of CO 2 emitted through energy consumption for biomass production, biomass logistics, ethanol processing, ethanol transport and distribution, and ethanol combustion, respectively, all in kg CO 2 •L -1 of ethanol produced.For α, 0.4204 was used [33].The C f was estimated using the relation C f = 0.069E f derived by regressing the energy use and CO 2 emissions values computed by the IBSAL model.The estimated C f value ranged from 0.13 to 0.15 kg CO 2 •L -1 , depending on biomass yield.These values were close to those of Spatari et al. [35] (0.12 kg CO 2 •L -1 ).The values of C l were estimated by dividing the IBSAL-computed emissions values (kg CO 2 •Mg -1 ) by γ.For C p , 0.13 kg CO 2 •L -1 was used [31].For C t , 0.05 kg CO 2 •L -1 was estimated using the GREET1_2011 software (http://greet.es.anl.gov).For C c , a value of 1.5 kg CO 2 •L -1 was used [35].

Statistical Analyses
The effects of soil and variety on the NEV and CCB of switchgrass-based ethanol production were determined using the Kruskal-Wallis test, a nonparametric alternative to the one-way Analysis of Variance (ANOVA) test and an extension of the Wilcoxon rank sum test to more than two groups.The Kruskal-Wallis test was used in place of the ANOVA because the assumption of normality was not met for each soil and variety.Tests were performed to find out if values of NEV and CCB were significantly different across soils and varieties.Such tests were carried out for each location and each of the three weather conditions: dry, wet, and average.Using medians, the Kruskal-Wallis test compares samples from two or more groups.The null hypothesis of the test is that all samples are drawn from the same population or from different populations with the same distribution.Its ANOVA table is calculated using the ranks of the data instead of their numeric values.The ranks are obtained by sorting the data from the smallest to the largest observation across all groups and taking the numeric index of this ordering.The test uses a chi-square statistic, whose significance is measured by the p value.The p value close to zero suggests that at least one sample median is significantly different from the others.For further information about which pairs of mean ranks were significantly different, the multiple comparison procedure was used with the Tukey-Kramer least significant difference test.

ALMANAC Evaluation
Values of the goodness-of-fit measures used to evaluate the ALMANAC model-the Root Mean Square Error (RMSE), the Willmott index of agreement, the Nash-Sutcliffe index (modeling efficiency), and the coefficient of determination (R 2 )-showed that the model worked reasonably well in simulating the switchgrass biomass yields for Mississippi (Figure 1).Although the yields of Cave-in-Rock, an upland cytotype, were slightly overestimated by the model relative to Alamo and Kanlow, lowland cytotypes, the overall agreement of the observed and model-estimated yields was good.

Soil Effect
The results showed significant difference in the NEV as well as CCB of switchgrass-based ethanol production among soils for all locations (Table 1).For a change from one soil to another, values of these variables changed by 15% -36%, depending on soil and location.In general, the values of NEV and CCB increased in the direction of heavier to lighter soils for all locations: clay   to silt loam for Tunica and Grenada and loam to sandy loam for Meridian (Figure 2).For Tunica, the values of NEV and CCB for loam soils were significantly higher than those for clay.Silty loam, sandy loam, and silty clay loam were not different, and neither were silty clay and clay.Silty clay was different from silty loam and sandy loam only.For Grenada, silty loam and silt had significantly higher values of NEV and CCB than silty clay loam and clay.Sand was different from only clay among the five soils.Silty clay loam was different from silty loam and silt but the same as sand and clay.For Meridian, only sandy loam values were significantly higher than those of loam.Sandy loam, sand, and loamy sand were about the same, and so were sand, loamy sand, and loam.The differences among the soils were likely due to variations in their physical and chemical characteristics [36].The establishment, growth, productivity, and survival of switchgrass were possibly affected by water holding capacity, which is defined by soil texture [8].Lighter soils with good water holding capacities and aeration produced more biomass yields.The effect of soil on CCB was the same as on NEV (Table 2) because these variables were linearly related: CCB = 0.1548NEV for Tunica, CCB = 0.1550NEV for Grenada, and CCB = 0.1556NEV for Meridian.

Variety Effect
In general, the values of NEV and CCB increased on the order of north-upland, south-upland, north-lowland, and south-lowland varieties for all locations (Figure 2).These results agree with those of Casler et al. [13].They observed the occurrence of similar pattern for the locations with latitudes of up to 40˚N, above which northlowland  varieties produced the largest yields of all varieties.In our study, the proportion of increase from southupland to north-lowland, however, was larger than those from north-upland to south-upland and from north-lowland to south-lowland, indicating that the inter-cytotype (lowland vs. upland) difference is larger than the interecotype difference within the cytotype (north vs. south) in the southern region of United States.The differences among the varieties were because upland (lowland) cytotypes have preferential adaptation to northern (southern) latitudes [13].That is, the yield advantage of a lowland (upland) cytotype in a southern (northern) location is greater than in a northern (southern) location.
Although the NEV and CCB values followed the above pattern, only north-upland and south-lowland varieties were significantly different from each other for all locations (Tables 1 and 2).This difference was likely because these two varieties belonged not only to different cytotypes (upland vs. lowland) but also to different ecotypes (north vs. south).The other pairs did not vary from one another because they belonged to either the same ecotype or the same cytotype.The varieties south-upland and north-lowland were also about the same because they belonged to the same origin (central Oklahoma), whereas south-lowland and north-upland were originated in the central/south Texas and central Great Plains, respectively [14].Several researchers, such as Sanderson et al. [5], Casler [13], Parrish and Fike [8], and Stroup et al. [37], found similar results.That is, upland cytotypes yielded substantially less than their lowland counterparts at all sites of their experiments.The larger yields in lowland cytotypes were because they are better adapted to the warmer and moister habitats of the southern region [4].The poor performance of the upland cytotypes compared with their lowland counterparts in the southern region was due to their daylength sensitivity and early maturity [5].The lowland varieties, on the other hand, matured later than their upland counterparts and thus produced larger yields [37].The larger yields of the more southern locations were likely due to longer growing season and or warmer temperature.Switchgrass is highly photoperiod-sensitive.Moving upland (lowland) cytotypes southward (northward) speeds up (delays) their reproductive maturity, shortens (extends) their growing season, and thus reduces (increases) yields [13,38].
The differences among upland and lowland cytotypes were influenced by the type of year (dry or wet).Gaps between lowland and upland varieties were smaller in a dry year than in a wet year for all locations (Figure 2).These results agree with those of Wullschleger et al. [39], in which differences between upland and lowland varieties were seasonally and environmentally dependent.In their study, upland varieties had shown less reduction in photosynthetic rates than their lowland counterparts in a dry year.Also in the study of Stroup et al. [37], upland varieties had exhibited less yield decrease than lowland ones under water stress conditions.This difference was because lowland cytotypes are associated with more hydric regions, whereas upland cytotypes are associated with more mesic regions.Upland cytotypes are more drought tolerant than their lowland counterparts [12,37].

Weather Effect
In general, the values of NEV and CCB increased on the order of dry year, average year, and wet year for all locations (Figure 2).These results were likely because wet years produced more biomass yields and thus larger values of these variables than did dry years.Under water stress conditions, switchgrass yielded less due to reduction in photosynthetic rates and leaf water potential [37].Accordingly, the NEV and CCB values in a wet year were significantly larger than those in a dry year for all locations (Tables 1 and 2).The difference between an average year and a dry or wet year, however, depended on location.For Grenada and Tunica, the average year was different from both wet and dry years, whereas it was about the same as dry year for Meridian.These differences were due to variation in precipitation distributions during the growing seasons of switchgrass.The distribution of precipitation in Meridian in an average year was not very different from that in a dry year, whereas the precipitation distributions among the years were different for the other two locations (Figure 3).
The differences between upland and lowland varieties were larger in a wet year than in a dry year (Figure 2).These results were similar to those of Sanderson et al. [5] and indicated that precipitation is a predominant factor affecting switchgrass productivity in southern locations.In their studies, the upland varieties from the U.S. Midwest region had matured earlier and produced less biomass than the lowland varieties from the southern region.For lowland cytotypes, precipitation is the most important climate variable [40].

Location/Latitude Effect
Generally, values of both response variables increased in the direction of south to north (Figure 2).The proportion of difference in this direction, however, was not the same for all locations.While the values of both NEV and CCB for Meridian were significantly different from those for Grenada and Tunica each, values for Grenada and Tunica were about the same.This variation was because Grenada and Tunica had similar soils (silt loam, silty clay loam, and clay) and similar precipitation distributions, whereas the soils (loamy sand and sandy loam) and the precipitation distribution in Grenada were different from those in the other two locations.as: [12]., where R is NEV or CCB, R is the value of R for location L and variety V, and LR is the value of R for location L. As the results showed, the R values for lowland types decreased, whereas those for upland types increased northwards (Figure 4).Thus, the differences among the varieties increased southwards although only north-upland and south-lowland varieties were significantly different.The results were in agreement with those of Casler et al. [13], who observed that upland (lowland) cytotypes had preferential adaptation to northern (southern) latitudes.They found that north-upland cytotypes had steeper positive slopes than their south-upland counterparts, whereas south-lowland cytotypes had steeper negative slopes than their north-lowland counterparts.Southlowland cytotypes tend to produce more than north-upland cytotypes in southern locations, whereas north-upland cytotypes tend to have larger yields than southlowland cytotypes in northern locations [5,13].Whereas upland cytotypes are generally better adapted to well drained soils in higher latitudes, lowland cytotypes are typically adapted to moist locations in lower latitudes  Results showed sig each across soils and varieties.Both NEV and CCB increased in the direction of heavier to lighter soils and on the order of north-upland, south-upland, north-lowland, and south-lowland varieties.Only north-upland and southlowland varieties were significantly different because they belonged to different cytotypes and ecotypes.Gaps between lowland and upland varieties were smaller in a dry year than in a wet year.The values of both NEV and CCB increased in the direction of dry to wet year.From south to north, they decreased for lowland cytotypes but

Figure 2 .
Figure 2. Net Energy Values (NEV) associated with various soils, weather conditions, and varieties for: (a) Tunica; (b) Grenada; and (c) Meridian in Mississippi.

Figure 3 .
Figure 3. Precipitation distribution during switchgrass growing season in a: (a) dry year; (b) average year; and (c) wet year for Meridian; (d) dry year; (e) average year; and (f) wet year for Grenada; and (g) dry year; (h) average year; and (i) wet year for Tunica in Mississippi, USA.The above differences among the locations were mainly due to variations in soil texture and precipitation.The absolute values of NEV and CCB, therefore, would not reflect the effect of latitude, if any, on these variables.Thus, normalized values of these variables were used instead of absolute values to eliminate location effects (soil and weather), if any.The normalized value of a re-sponse variable R for location L, denoted as , was computed L R N

Figure 4 .
Figure 4.The normalized values of Net Energy Value (NEV

)
and carbon credit balance (CCB) belonging to four cytotypes for three locations in Mississippi, USA.