Pin Oak ( Quercus palustris ) Leaf Extract Mediated Synthesis of Triangular , Polyhedral and Spherical Gold Nanoparticles

Fabrication of phytogenic metal nanoparticles is a developing area of nanobiotechnology which has economic and potentially eco-friendly advantages over conventional chemical and physical synthesis methods. We report the synthesis of anisotropic gold nanostructures using a phytoextract from the leaves of the pin oak tree, Quercus palustris. We used 2.5%, 5%, and 10% m/v extract concentrations to determine the importance of pin oak phytoextract concentration on the properties of the nanomaterials synthesized. Characterization of the resulting nanomaterials revealed that the concentration of the phytoextract is a key factor controlling the size and relative frequency of shapes the gold nanostructures formed. TEM micrographs demonstrate that triangular, spherical, and polyhedral gold nanostructures were formed in all treatments, although UV-Vis spectra indicated that the formation of fewer nanostructures in the 2.5% m/v phytoextract treatment We found that anisotropic nanostructures are formed at the highest concentrations in the preparation using 10% m/v phytoextract. UV-Vis, TEM, and DLS data indicate that the 5% m/v phytoextract results in the synthesis of the smallest sized nanoparticles.


Introduction
Nanoparticles (NPs) are of great interest due to their small size and large surface area to volume ratio.The most common method of synthesizing noble metal nanostructures is via reduction of the metal salt with a suitable reducing agent in the presence of a stabilizer [1].However, the ability to synthesize anisotropic noble metal nanostructures with high yield remains a challenge as the prevailing protocol requires complex and time-consuming steps.Moreover, there is a growing demand for the development of clean, non-toxic, bio-compatible and ecofriendly methods for the synthesis of metal NPs.Biogenic synthesis methods are generally considered to be safe, cost-effective, and adaptable to the large scale production of metallic nanostructures.
Current trends in metal nanostructure synthesis focus on monodispersity of size and selectivity of shape, both of which are the key for manipulating the unique magnetic, catalytic, optoelectronic, and mechanical properties of nanomaterials [2].Control of the particle shape and size is essential for modified surface plasmon resonance (SPR) properties exploited for the development of optical and near-infrared metamaterials [3].Gold (Au) and silver (Ag) nanomaterials exhibit important shape dependent properties [2,4].Nanotriangles exhibit two or more prominent absorption bands, one a transverse absorption band in the visible region and the other a longitudinal absorption band in NIR region, which are similar to properties observed for nanorods [4,5].The NIR region is of significant biological importance since it represents the only spectral window for both therapeutic and imaging applications in biological tissue which do not have significant absorption in the NIR region.Therefore, Au nanotriangles with strong NIR absorption could find potential applications in such areas as surface enhanced raman spectroscopy (SERS) substrates [6], infrared radiation absorbing optical coatings [7], and wave guides for electromagnetic radiation.Further, Au NPs have been used in protein assays [8], immunoassays [9], capillary electrophoresis [10], and as thermal scalpels to kill cancer cells [11,12].
Biosynthesis of both isotropic and anisotropic NPs has been demonstrated with a variety of biomolecules including chitosan [13], enzymes [14] and plant extracts.Trian-gular and truncated triangular Au NPs were synthesized by Chandran et al. (2006) using Aloe vera plant extract [15].Triangular Au NPs have also obtained by the reduction of Au ions by lemongrass extract [16].In both studies, freshly cut plant material was boiled into a broth to synthesize the phytoextract, the concentration of which was found to influence NP size as well as the amount of triangular NPs produced.Compared to this broth, sundried/shade-dried biomass has the advantages of convenient preservation and storage in addition to requiring less energy to produce.Synthesis of anisotropic nanomaterials using sun or shade dried plant biomass has been reported by Ghosh et al. (2011) using Dioscorea bulbifera tuber extract [17] and by Fayez et al. (2011) using Cinnamomum camphora leaf extract [18].In the former study, the researchers also found that the shape and size of the nanomaterials produced was influenced by reaction time and reaction temperature, with 50˚C being the optimal temperature.The importance of reaction time and temperature in the formation of anisotropic nanostructures was also reported by Chili et al. (2008), who demonstrated polymer mediated synthesis of anisotropic Au NPs [19] and by Shao et al. (2004), who demonstrated aspartate mediated synthesis of triangular and hexagonal nanostructures under room temperature conditions [20] whereas the same reaction did not yield traingular and hexagonal structures when synthesized at a boil [21].
We have used a phytoextract from shade-dried leaves of the pin oak tree, Quercus palustris, at varying concentrations to synthesize Au nanostructures at room temperature.The objectives of the study were to investigate whether pin oak leaf extract from shade dried oak leaves will induce formation of anisotropic Au nanostructures at room temperature and to determine differences in particle size and shape as a function of phytoextract concentration.The results of this study are the first data evaluating the use of Quercus palustris extract for use in green nanomaterial synthesis and could have important implications for the development of cost-effective large scale production of anisotropic Au nanostructures.

Materials and Methods
Chloroauric acid (HAuCl 4 •3H 2 O) was purchased from Sigma-Aldrich and used as received.Pin oak leaves (Quercus palustris) were collected in August from the Lexington Arboretum in Lexington, Kentucky USA.Pin oak is a common ornamental tree native to the eastern United States with limited economic importance [22].Leaves were thoroughly washed with tap water and allowed to air dry in the shade, after which the leaf tissue was removed from the stems.The leaf tissue was finely ground using a mechanical plant tissue grinder.Oak leaf extract was prepared by adding 2.5, 5, or 10 g ground oak leaves to 100 mL doubly deionized water (DDI) water and heated to 100˚C for 45 minutes.Following the extraction solutions were allowed to cool to room temperature.
Five mL of 2.5%, 5.0%, and 10.0% m/v oak leaf extract were added to 95 mL of 10 -3 M aqueous HAuCl 4 •3H 2 O solution.The reaction mixtures were allowed to stand at room temperature for 24 h.Suspensions were centrifuged at 2250 × g for 10 min and the supernatant decanted.The concentration of the resulting suspension was determined by dissolving an aliquot of each suspension in concentrated aqua regia and measuring the amount of Au present in a diluted sample via inductively coupled plasma mass spectrometry (ICP-MS) using an Agilent 7500 cx ICP-MS (Agilent, Santa-Clara, CA, USA).Additionally, 3 mL aliquots of each nanoparticle suspension were filtered through a 3 kDa Amicon Ultra membrane (Millipore, Billerica, MA).These samples were also analyzed for Au using ICP-MS to determine the concentration of dissolved Au in the nanomaterial suspensions.
UV-Vis spectroscopic measurements of the synthesized Au nanostructures were carried out on a Varian model 50 Bio dual-beam spectrophotometer (Agilent, Santa-Clara, CA, USA) operated at a resolution of 1 nm.Hydrodynamic diameter and electrophoretic mobility measurements of the Au nanostructures were measured with a Nano-ZS zetasizer using 173˚ backscatter analysis method (Malvern, Worcestershire, UK).FT-IR measurements of the oak leaf extract and Au NPs synthesized using different concentrations of the extract were carried out on a Thermo-Nicolet 6700 (Thermo Scientific, Waltham, MA, USA) in the diffuse reflectance mode operated at a resolution of 8 cm -1 .Filtrate samples were also analyzed with filtrate spectra subtracted from suspension spectra.TEM samples were prepared by pipetting drops of each NP suspension on 200 mesh carbon coated formvar copper grids and allowing the sample to dry by evaporation.TEM size analysis of the NPs was performed using a Jeol 2010F HR-TEM operating at 200 keV.Particle size and shape distributions were quantified based on measurements of at least 100 particles from at least 3 different micrographs using ImageJ software.

Results and Discussion
After adding the phytoextract to the Au chloride solutions, the color of the solution changed from pale yellow to dark red immediately on mixing in all treatments, indicating formation of Au nanostructures.The SPR band observed near 530 nm [11] in all three treatments confirms the reduction of Au 3+ and the formation of Au NPs (Figure 1).However, despite the fact that a peak exists in all treatments near 530 nm, the peak for the 2.5% m/v phytoextract treatment is very weak, suggesting that fewer nanostructures were formed in this treatment that in the 5% and 10% m/v treatments.The peak absorbance for the nanoparticle suspensions occurs at 584, 537, and 555 nm for the 2.5%, 5%, and 10% m/v treatments, respect-tively.The frequency and width of the SPR absorption peak depends on the size and shape of the metal NPs as well as on the dielectric constant on the metal itself and the surrounding medium [23].For Au NPs with a mean diameter larger than 25 nm, the absorbance maximum of the SPR band increases with increasing mean NP diameter [23,24].For Au NPs with a mean diameter smaller than 25 nm, the trend reverses and the SPR band absorbance maximum increases with decreasing mean NP diameter [24].Alternately, the increase in absorbance wavelength for the 2.5% m/v treatment could be indicative of a mean diameter smaller than 25 nm.Since the peak absorbance for the nanomaterial suspendsion synthesized with 5% m/v smallest phytoextract occurred at the shortest wavelength, these data indicate that the 5% m/v phytoextract yielded the NPs.The absorption spectrum for the 10% m/v treatment is broad approaching the NIR region compared to the other two treatments, suggesting a relatively high amount of anisotropic NPs [7].This result differs from other studies examining the effect of increased phytoextract concentration on nanomaterial synthesis that found that particles synthesized with high concentrations of phytoextract had minimal absorbance in the NIR region [7,15].Although the reasons for these contradictions are unclear, they are possibly related to the relationship between NP suspension surface area and phytoextract concentration used in each individual study.We speculate that increasing phytoextract concentration will stabilize NP suspensions to a point corresponding to adequate NP surface coverage, after which increasing phytoextract concentration will begin to destabilize the suspension and promote aggregation, increasing NP size and resulting in a red shift in UV-Vis absorbance.This hypothesis is supported by a recent study that reported a decrease in NP size and a blue shift in absorbance as a result of increasing the volume of olive leaf phytoextract added from 0.2 to 2 mL but an increase in particle size and red shift in absorbance when the volume of phytoextract added was increased from 3 to 6 mL [25].
Measurements of electrophoretic mobility reveal that the nanoparticle suspensions synthesized with 2.5 and 5% m/v phytoextract have relatively similar zeta potentials, -34.2 and -41.9 mV, respectively.Hydrodynamic diameter measurements reveal mean diameters of 124.4,46.1, and 50.2 nm for the 2.5, 5, and 10% m/v treatments, respectively.The polydispersity index of the NPs increases with increasing phytoextract concentration (Table 1).The negative zeta potential values revealed by electrophoretic mobility measurements are indicative of high negative surface charges which provide stabilization of the particles in the form of electrostatic repulsion from other particles.However, the suspension synthesized with 10% m/v phytoextract has a zeta potential of only -13.8 mV, indicating these particles have a lower degree of charge stabilization.
FTIR spectra reveal peaks at approximately 780, 1635, 3250, and 3460 cm -1 in the phytoextract as well as in all three treatments (Figure 2(a)).FTIR spectra from suspension filtrate were subtracted from these initial spectra to investigate the molecules specifically associated with the nanomaterials (Figure 2(b)).In these subtraction spectra, only peaks at approximately 780 and 3250 cm -1 are present.In FTIR spectra of the original samples, the sharp peaks at approximately 3460 cm -1 and the peak at approximately 1635 cm -1 are both likely due to the presence of N-H bonds in either amide or amine groups [25].In the subtraction spectra, the peak at 3250 cm -1 is likely due to the presence of phenolic hydroxyl groups [15] and the peak near 780 cm -1 likely due to the presence of aromatic compounds [26].This result indicates that molecules with these functional groups are associated with the NPs.
TEM images of the NPs synthesized with the 2.5% m/v phytoextract reveal that the suspension was predominately composed of masses of heavily aggregated nanostructures (Figures 3(a)-(c)).These structures were not used in the calculation of mean diameters and the distribution of shapes within the suspension.Superimposed on these is a relatively sparse population of larger nanospheres and anisotropic nanostructures with a mean diameter and standard deviation of 58.2 ± 20.9 nm (Table 2).The nanoparticle suspension synthesized with the 5% m/v phytoextract consists predominantly of spheres with    1).Unlike the NPs synthesized with the 2.5 and 5% m/v phytoextract, there is no evidence for two different size populations of NPs in the 10% m/v suspension.TEM micrographs revealed the synthesis of anistotropic nanomaterials in each treatment, although there were large differences between NPs depending on treatment.The 5% m/v extract produced the smallest NPs and the 10% m/v extract produced the highest proportion of anisotropic nanostructures.Wiley and co-workers (2005) observed that nanorods evol e from multiply twinned v particles (MTPs) as a result of anisotropic growth caused by certain shape-directing agents [27].We speculate that, in our study, the anisotropic structures observed could result from MTPs initially formed under slow reaction conditions that subsequently undergo shape transformations, eventually evolving into Au anisotropic structures as a result of the shape-directing constituents within the oak leaf extract.We speculate that a higher concentration of these shape directing agents in the 10% m/v extract treatment resulted in the higher amount of anisotropic nanostructures.The sintering of spherical NPs at room temperature may also lead to the formation of anisotropic structures.A similar kind of crystal growth was observed under time dependent electron beam exposure to the Au NPs made with 2.5% extract (Figure 4).It is possible that spherical Au NPs were initially formed at the reaction products which evolved to Au nanotriangles and polyhedral nanostructures as a result of reduction, assembly, and room temperature sintering [28].Alternatively, the difference in the growth rates of the various crystallographic planes could direct the morphology of the NPs [29].The formation of different structures likely results from triangular platelets forming when there is one twinning plane parallel to the plane of the platelet Copyright © 2012 SciRes.ANP resulting in one set of fast growing concave edges [3], similar to the silver halide model [30,31].Hexagonal platelets have been shown to result from two twinning planes which yield more highly symmetric growth [30,31].

Conclusions
We have demonstrated for the first time the room temperature synthesis of Au nanospheres, nanopolyhedrons, and nanotriangles using phytoextract from shade-dried leaves from Quercus palustris.This synthesis protocol allows for the substitution of oak leaf phytoextract, a relatively eco-friendly and inexpensive bioreductant, for chemical reductants such as sodium citrate or sodium borohydride.Additionally, we have demonstrated the ability to synthesize Au NPs using shade dried leaves from a plant with little current economic importance.We have also demonstrated that manipulation of nanoparticle size and shape can be achieved by adjusting the concentration of oak phytoextract.The results of this study could have important practical implications for the development of cost-effective and green processes for the large scale production of anisotropic Au nanostructures.

Figure 2 .
Figure 2. (a) FTIR spectra characterizing nanoparticle suspensions and oak leaf extract (b) Suspension spectra with filtrate spectra subtracted.

Figure 4 .
Figure 4. TEM micrographs of the response of gold nanoparticles synthesized using 2.5% m/v oak extract to exposure to the electron beam; (a) t = 0, (b) t = 60 s, (c) t = 180 s.