Children with Pneumonia Caused by Streptococcus pneumoniae Resistance Analysis and Clinical Features

Objective: To analyze the causes of childhood pneumonia Streptococcus pneumoniae resistance and clinical characteristics, and provide a basis for better and timely clinical therapy, and medication to reduce blindness. Methods: MIC method in our hospital 114 under 2020 pediatric pneumococcal respiratory infection in children with lower respiratory tract specimens were isolated antimicrobial susceptibility testing, and analyzed retrospectively. Results: 84 male children, 30 female children, the largest of which 9 years old, the youngest two months, infants less than 1 year old, 90 people; suffering from bronchial pneumonia, 90 cases, 21 cases of pneumonia, wheezing, 3 cases of bronchitis, the average length of stay for about a week; improved in 79 cases, 33 cases were cured, 2 cases transferred to higher level hospitals. All children with throat congestion, swollen tonsils, lung breath sounds rough, smell and moist rales. 114 penicillin-resistant streptococcus pneumoniae was 64.9%, erythromycin 97.4%, clindamycin 86.8%, tetracycline 87.7%, trimethoprim-sulfamethoxazole 82.5%, amoxicillin 21.9%, cefotaxime 49.1%, chloramphenicol 10.5%, was not found to levofloxacin and vancomycin. Conclusion: Penicillin, erythromycin, and clindamycin are not as pneumococcal pneumonia in children experience preferred medication in children less than one year old child could easily cause lung chain streptococcus pneumonia. Therefore, the antimicrobial resistance of Streptococcus pneumoniae analysis provides a reference for experienced clinicians to adjust medication.


Introduction
Streptococcus pneumoniae is one of the most common pathogens causing acquired respiratory tract infections in children. It is not only the pathogen of lobar pneumonia and bronchopneumonia, but also can cause otitis media, mastoiditis, sinusitis, meningitis and bacteremia. Its invasiveness is mainly capsule. The virulence is weakened or lost if the capsule is lost. Hemolysin and neuraminidase are also the main pathogenic factors [1]. In recent years, with the extensive clinical application of broad-spectrum antibiotics, the drug resistance of clinical isolates of Streptococcus pneumoniae is becoming more and more serious, and there are great differences between different regions [2]. Strains can produce drug resistance through selective pressure mutation of antibiotics and horizontal gene migration [3]. In order to reduce medication blindness and guide clinical rational drug use, the drug resistance and clinical characteristics of 114 strains of Streptococcus pneumoniae isolated from lower respiratory tract specimens in pediatrics of Liuyang Hospital of traditional Chinese medicine in 2020 were retrospectively analyzed.

Clinical Material
In 2020, 114 strains of Streptococcus pneumoniae were isolated from the sputum of children with lower respiratory tract infection in Liuyang hospital. The ethics approval number was YAH011.

Sputum Collection
Apply lubricating oil to the top of the sputum suction tube. The child lies on his back. Insert the sputum suction tube into the throat through the pharyngeal cavity or respiratory tract, and connect with the negative pressure sputum suction device to absorb sputum. Sputum was screened under a microscope. If the white blood cells of the specimens were larger than 25/LP, epithelial cells were less than 10/LP or 10 -25/LP, the culture medium would be further inoculated.

Bacterial Isolation
The inoculated medium was placed in the incubator at 35˚C and incubated in 5% CO 2 for 18 -24 h. then, the slime colonies with gray, flat, moist, grass-green hemolytic ring, central depression and umbilical fossa or oil drop like colonies were picked out. Apply it on the blood plate with 5 μ After incubated at 35˚C and 5% CO 2 for 18 -24 h, the positive colonies were defined as the diameter of inhibition zone ≥ 14 mm.

Identification and Drug Sensitivity
The positive strains in optochin disk test were identified as Streptococcus pneumoniae by rapid ID32STREP identification card read by French merier ATB semi-automatic identification analyzer, and the quality control strain was Strep-

Statistical Treatment
Statistical comparison was performed by one-way ANOVA preceded by LSD test. The level of significance for all analyses was set at p < 0.05 SPSS 16.0 (Inc., USA) statistical package for Windows was used for data analysis.

Clinical Data
Among the 114 cases, 84 were male and 30 were female. Among them, the oldest was 9 years old and the youngest was 2 months old.

Antimicrobial Resistance of Streptococcus pneumoniae
The drug sensitivity test results of Streptococcus pneumoniae to antibiotics are shown in Table 1. A total of 114 strains of Streptococcus pneumoniae were    Compared with 68 cases of adult isolated Streptococcus pneumoniae in our hospital, the drug resistance rate of children to Streptococcus pneumoniae was higher than that of adults, and the detection rate was also higher than that of adults.
According to the resistance, mediation and sensitivity of Streptococcus pneumoniae strains to penicillin, Streptococcus pneumoniae strains were divided into 74 strains in PRSP group, 29 strains in PISP group and 11 strains in PSSP 1 group three ( Table 2). The drug resistance rate of PRSP group was significantly higher than that of PISP and PSSP groups.

Discussion
The positive rate of Streptococcus pneumoniae was higher in children and adults with young relatives or nursery children, smoking habits, asthma or acute respiratory infection [4]. This study showed that 79% of the children were diagnosed with bronchopneumonia, which was consistent with Streptococcus pneumoniae as the main pathogen of bronchopneumonia. The isolation rate of Streptococcus pneumoniae in children with respiratory tract infection ranged from 5.1% to 40.5% [5] [6] due to WHO estimates, in 2008, 8.8 million children under the age of 5 died worldwide, of which 476,000 died from Streptococcus pneumoniae infection. In developing countries, the mortality rates of Streptococcus pneumoniae sepsis and meningitis were 20% and 50% respectively.
With the wide spread of drug-resistant strains in the world, the rate of antibiotic resistance is increasing. The emergence of multidrug-resistant Streptococcus pneumoniae brings severe challenges to clinical treatment [7]. This study showed that the resistance rate of Streptococcus pneumoniae to penicillin reached 64.7%, higher than 25.12% [8], and lower than 96.4% [9]. Five high molecular weight PBPs (pbps1a, 1b, 2x, 2a, 2b) and one low molecular weight protein [10] were found in Streptococcus pneumoniae. The affinity of penicillin to pbp2B of Streptococcus pneumoniae was stronger than that of the fourth generation cephalosporins.
The resistance rate of Streptococcus pneumoniae pneumonia to erythromycin, clindamycin and tetracycline was 97.4%, 86.8% and 85.3%, respectively, which were lower than those reported [8] [9] [10] [11]. According to these data, macrolide antibiotics can no longer be used as the first choice of experiential medication for children with community-acquired pneumonia.
The resistance rate of Streptococcus pneumoniae to antibiotics commonly used in children is high, and the situation is not optimistic [12] [13]. While antibiotics have cured and saved many patients' lives, there has been a gradual increase in bacterial resistance and the emergence of multi-drug-resistant strains due to the irrational use of antibiotics. In recent years, a rapid diagnostic reagent for Streptococcus pneumoniae antigen has been developed, which mainly detects Streptococcus pneumoniae antigen in urine, cerebrospinal fluid or pleural effusion [14] [15].

Conclusion
To sum up, in the face of the complex drug resistance of Streptococcus pneumoniae, there is a long way to go for the continuous monitoring of drug resistance, the control of the spread of multi-drug-resistant strains and the rational use of antibiotics.