The Impact of Some Environmental Factors on the Distribution of the Benthic Ostracod Species from of Safaga Island, Red Sea, Egypt

The benthic ostracods of Red Sea of Egypt have received little attention in ecological studies. Temperature, depth, salinity, dissolved oxygen, pH, conductivity, and other environmental variables all have an impact on benthic ostracods. This study aimed to determine the influence of environmental parameters on the distribution of benthic ostracods by determining the similarity and dissimilarity between the eight collection sites, investigating the regional distribution form by accomplished Multidimensional Scaling (MDS), and recognizing the percentage of the influence of each species on the resemblances and variances within the clusters formed by SIMPER analysis, determining the relationships between depth and other factors by Spearman’s rank correlation coefficient. A total of 43 ostracod species had been identified. According to the frequency index, a single species was rare, six were common and the remaining species (36) were dominant. Seven ostracods species were found across all sites and Cytherelloidea sp. was found in two. The abundance and richness of ostracod species were correlated positively with water temperature, dissolved oxygen, organic matter, salinity, and calcium carbonate. The cold water is preferred by the ostracods Cylindroleberis vix and Prionotoleberis lux and the warm water is favored by podocopid ostracods. The findings of this study will aid in the identification of ostracod species, as well as understanding the characteristics and ecological variables in this zone of the Red Sea in Egypt. The current investigation is an attempt to shed the light on the features of ostracods that live on the east side of Safaga Island.


Introduction
The Ostracoda is a class of Crustacea characterized by a bivalved carapace, which encloses the body and appendages. Ostracods occur in almost all types of water bodies, and they are one of the most abundant groups of benthic microfauna. In addition, they distribute almost everywhere from shallow marine to deep-sea environments, and they are useful as indicators of the environmental parameters variation [1].
Generally, the controlling factors on marine ostracods are water temperature, depth, salinity, dissolved oxygen, pH, alkalinity, food supply and sediment organic matter content [2]. To testify this, Mesquita-Joanes et al. [3] maintained that temperature is the most important factor affecting ostracod growth and survival. Furthermore, Ruiz et al. [4] stated that salinity was the most important factor in species distribution. Also, Ruiz et al. [4] stated that ostracods may be affected by pH and electrical conductivity.
Though ostracod species are numerous and abundant in the Egyptian Red Sea region, benthic ostracods have received little attention in ecological studies.
What we have are only some studies on the recent benthic marine ostracods of the Red Sea, such as [5] which dealt only with Cypridinidae, [6] characterized the ostracod community of Al Gardaqa, and [7] investigated the ostracod assemblages of the Abu Dhabi lagoon. In addition, [8] and [9] investigated ostracods from the Gulf of Aqaba, [10] and [11] gave information on foraminifers and ostracods from the Jordanian Gulf of Aqaba. Also, [12] gathered data on benthic ostracods from El Hameira (Gulf of Aqaba) and [13] collected data on ostracods from Safaga Bay.
The goals of this study were to identify and determine the ostracod assemblages that live between 0.5 m to 30 m depths on the east side of Safaga Island, to determine the ecological parameters of the environment in which these species live and to figure out the impact of these ecological factors on the distribution and abundance of ostracod species according to the seasons.

Materials and Methods
In the current study, 32 samples are collected by using the nylon net (150 μm mesh size) and Van Veen grab from eight stations of the east side of Safaga Island ( Figure 1 and Table 1 The ostracod species studied in this work were not listed in the IUCN Red List of Threatened Species (http://www.iucnredlist.org/) [14]. No permits were required for the labeled study, which conformed to all applicable guidelines.    [9], Bonaduce et al. [6] [15] [16] [17] [18] and the help of Australian Museum. In the current study, the frequency of the ostracod species was calculated by using [19] frequency index.
The results were evaluated as dominant (F ≥ 50%), common (50% > F ≥ 25%) and rare (F < 25%). To determine the similarity between the eight collection sites, Bray-Curtis Similarity Index was done. The Bray-Curtis dissimilarity was used to measure the variances in species populations between two different sites and can be calculated with the following formula: Multidimensional Scaling (MDS) was accomplished to investigate the regional distribution form [20]. SIMPER analyses were done to recognize the percentage of the influence of each species on the resemblances and variances within the clusters formed after mass analysis. Spearman's rank correlation coefficient was done to determine the relationships between depth and other factors.  4.11 to 9.6 mg/L and the conductivity values ranged from 60.1 to 64.6 ms/cm. As well as, total calcium carbonate ranged from 50% to 89%. Also, TDS ranged between 33.4 to 39.8 g/l and organic matter varied from 8.9% to 24.6%.

Ostracod Species and Seasonal Distributions
The collecting ostracods (39 species of Podocopida and four species of Myodocopida) identified in eight collecting sites on the east side of Safaga Island, as well as their Frequency Index values, were shown in Appendix, Table 3, and

Species Abundance
According to the seasonal collecting samples, 43 of ostracods were collected and identified (Figures 3-6). Furthermore, the highest number of ostracod species

Bray-Curtis Similarity and Multidimentional Scaling (MDS) Ordination
The dendrogram of Bray-Curtis similarity and Multidimentional scaling (MDS) ordination of the collecting sites was formed of Bray-Curtis average clustering and the similarities between the stations were displayed in groups and shown in Figure 8 and Figure 9. The groups that exhibited the highest similarity and difference in each season were determined according to SIMPER analysis results. In summer, the similarity revealed three groups; the similarity within the first group was 42.3%, within the second group was 41% and was 59.5% within the third group. In autumn, the similarity displayed two groups; the similarity inside the first group was 42.1%, while that similarity was 34.2% within the second group. Furthermore, the similarity between the two groups was 38.5% and 40.3% of the first and second groups, respectively, during the winter season. In spring,    The correlation among measured parameters such as water temperature, pH, salinity, dissolved oxygen, conductivity, total calcium carbonate, TDS, total organic matter, individual number, richness and depth were determined using Spearman's rank correlation coefficient method and listed in Table 5. The results of correlation analysis displayed that the individual number and richness of ostracods were positively correlated with water temperature, dissolved oxygen, organic matter, salinity and total calcium carbonate, and the individual number of ostracods was negatively correlated with the depth of sea water.

Discussion
The results of this study confirmed the presence of distinct difference in the abundance of ostracod species between the eight collecting sites. The shallow sites (site 1 and 2) were characterized by higher densities than those of the benthic sites, this is chiefly related to the location of sites in an area contains seagrass, sea beds and macro-algae, thus the availability of food for ostracods. [21] recorded that some ostracod species living in close proximity to sea grasses, algae, and mangrove trees, which are abundant along the Red Sea's coast. Moreover, [21] recorded that the ostracod species with higher percentages are associated with algae and seagrasses. The leaves promote the growth of macroalgae, which provide food and shelter for invertebrates, allowing them to reach significantly higher densities than in unvegetated benthic habitats [22]. According to Xie et al. [23] the benthic aquatic is not suitable for ostraod surviving because the decreasing of plants and reducing of water transparency.   Distribution and abundance of ostracod species and specimens are related to numerous diverse ecological factors (Batmaz et al., [24]). The ecological parameters (temperature, dissolved oxygen, pH, salinity, CaCO 3 (%) and TDS) and the physical characteristics (depths, aquatic plants and predation) control the occurrence and abundance of ostracod species [25] [26].
According to the results of Spearman's rank correlation (Table 5), the individual number of ostracod species was correlated positively with temperature.
Also, [27] noted that the species occurrence was positively related to both water temperature and electrical conductivity. In addition to this, Mesquita-Joanes et al. [3] discovered a high positive association between water temperature and ostracod population expansion, suggesting that temperature is important for species survival. Correspondingly, [28] and [22] noted that no direct relationship occurs between the distribution of ostracod species and temperature. [29] recorded that, water temperature and salinity are the major controlling factors governing ostracod distribution in estuarine environments. Moreover, a positive correlation was observed between individual numbers and richness and salinity, according to the correlation of Spearman's rank (P < 0.01) ( Table 3). [25] [26] suggested that salinity is an active factor in the diversity and the abundance of ostracod species. In the same way, [30] approved that salinity has a significant effect on the diversity of ostracod species.
In the current study, dissolved oxygen ranged from 4.11 to 9.6 mg/L and it correlated positively with individual and species numbers. According to Ahmed et al. [31] the higher values of dissolved oxygen during some months are owed to increase the activity of the photosynthesis process, while lower values might be because of using the organic substances and breathing of organisms. Moreover, [32] noted that dissolved oxygen is not an important factor within an aquatic ecosystem and it is the smallest effective analyst for the ostracod species, respectively. On the other hand, Külköylüoglu et al. [33] [34] noted a positive correlation between individual numbers of the species and dissolved oxygen. The pH of water of the current study does not fluctuate greatly at the different depths during the four seasons. Similarly, data noted by Sridhar et al. [35].
From this study, it is concluded that the relationship between abundance and richness of ostracod species were positive with total organic matter and total calcium carbonate. A similar observation was noted by [36]. [37] noted that the greater portion of the calcium carbonate content was made by the carbonate shells of some organisms like molluscs, foraminifera and algae.
The Bray-Curtis Similarity Index and MDS of the current result revealed that, the highest similarity in summer (59.5%) in the third group at sites 2 and 3. Furthermore, the highest similarity in autumn (42.1%) inside the first group was noted at sites 7 and 8. In other words, the highest similarity in winter (40.3%) was determined in the second group at stations 1 and 3. As well, the highest similarity in spring (44%) in the first group was recorded at sites 7 and 8. However, Bray-Curtis similarity index of some ostracod spcies from the Sea of Marmara showed five clustering groups and the level of similarity is very associated with habitat type and depth (Paçal et al., [38]).
The present collected ostracods categorized as follows: Cytherelloidea sp. was rare, sixteen species were common and the remaining species (26) were dominant. In the Adriatic Sea, Loxoconcha agilis was found to be the dominant species between 20 and 60 metres deep [28]. [21] recorded four rare species (Leptocythere arenicola, Cytherelloidea sp., Paranesidea n. sp., and Cytherois gracilis) in the intertidal zone of the mangrove ecosystem of the Red Sea Coast. During four seasons, 32 samples were collected from 8 sites, where the individual numbers of ostracod species were biggest in summer and the lowest number were recorded in winter. Moreover, the richness of ostracods was close to each other in all seasons and all sites, except in the eighth site (30 m) where the richness was very low. The current study showed that the number of podo-Open Journal of Marine Science copid ostracods increase in low depths and decrease in high depths so, they favored the warm water. [39] discovered that the ostracods they collected were rare and only found in the warm waters of the E and NE shelves. Further, the individual number of Cylindroleberis vix and Prionotoleberis lux increases in deep water and decreases in shallow water, indicating that they prefer cold water. [19] discovered that Cylindroleberis vix at 15 to 20 m depth in the Mozambique Channel, Mayotte, and Prionotoleberis lux at 300 to 350 m depth near Mayotte, Indian Ocean.
The temperature is well known as the most important factor in increasing reproduction, and breeding periods influence the seasonal dominance of species.
Orth et al. [40] noted that the low water temperatures from 15˚C to 20˚C resulted in the longest survival times, but high water temperatures between 20˚C to 24˚C provided the highest rates of moulting and shortest intermoult times. Also, in several crustacean species, higher temperatures determine an increase in the molt increment during the juvenile phase, while during the mature growth phase the reverse is true [40] [41]. [42] found that in small animals, the link between temperature and intermolt shortening is strong, but bigger animals are less responsive to temperature changes.
The data gained from the current study recommend that ostracod species have different tolerance values to many factors in variety of depths except Cytherelloidea sp. (Külköylüoglu et al., [33]; Külköylüoglu et al., [44]; Yavuzatmaca et al., [45]) support this datum.

Conclusions
In this study, 6733 ostracod individuals belonging to 43 species were collected seasonally from the east side of Safaga Island. The effects of some environmental factors on the collected ostracod species were determined in the eight collecting sites (see Table 2). The current results show that ostracod species have been affected by temperature, salinity, conductivity, pH, dissolved oxygen, TDS, and organic matter and CaCO 3 at eight sampling stations. The highest number of ostracod individuals and richnesss were observed in summer, while the lower ones were recorded in winter. Furthermore, the statistical analyses were used to assess the influence of ecological factors on the present ostracod species at the various collecting sites. The study of benthic ostracod environment relationships allows us to better understand the environmental characteristics, distribution and identification of the ostracod species found on the east side of Safaga Island at depths fluctuating from 0.5 to 30 m, providing variable information about biotic and abiotic ostracods patterns.