Genetic Diversity with Cluster Analysis of Maize Genotypes ( Zea mays L.)

The research investigation was carried out in the experimental area of Sher-e-Bangla Agricultural university, Sher-e-Bangla Nagar, Dhaka-1207, during the late Rabi season Mid December to May 2018 to study the genetic diversity with cluster analysis for the 35 maize genotypes as experimental materials that were laid out in Randomized Complete Block Design (RCBD) with three replica-tions. The research work was oriented to calculate and estimate the yield fac-tor through analyzing genetic diversity involving the yield contributing characters. The maximum yield per plant (117.51 g) was estimated in the genotype G12 (Pacific) and the minimum yield per plant (51.89 g) was recorded in the genotype G17 (Dekalb Super). Due to the crossing among the 35 maize genotypes, a wide range of divergence was observed in this experiment. The highest genotypes were included in cluster number V with 12 genotypes: BHM-5, PAC-60, Pacific-98, HP-222, Khai Bhutta, AS-999, Pioneer, Duranta, Kaveri 218, Chamak-07 and Golden-984. Here, the intra cluster distance was observed in cluster I (1.23), II (0.00), III (0.76), IV (2.08) and V (1.89) respectively. The highest intra cluster was recorded in cluster IV (2.08) and the lowest in


Introduction
Maize (Zea mays L.) is the 3 rd most significant cereal crop after wheat and rice in the world [1]. It is also commonly known as Corn (mostly in North America) originates in the Andean region of Central America and is basically first domesticated by indigenous people in southern Mexico. After domestication, maize spread quickly through North and South America, reaching the northeastern US and southern Canada prior to European colonization. Maize spread rapidly throughout Europe and from Europe to other parts of the world after the European discovery of the Americas [2]. Maize (Zea mays L.) is an important staple crop of the world after wheat and rice. At the same time, it is a versatile crop due to its multifarious uses as feeds, food and industrial raw material. The crop serves as a source of basic raw material for a number of industries viz., starch, protein, oil, alcoholic beverages, food, sweeteners, cosmetics and biofuels [3].  [5]. So, the production of maize in Bangladesh is continuously increasing. Among the different districts in Bangladesh-Dinajpur, Chuadanga, Takurgaon, Lalmonirhat, Rajshahi, Kushtia, Rangpur and Bogra are noted to be more progressive in maize production with higher rates of growth. Both composite and hybrid maize are grown well in the loam and sandy-loam soils of the country (with three to four irrigations).
Hybrid maize has a greater yield with 2.4 tons/acre (5.4 tons per ha), which is higher by one-third over composite maize (1.47 tons/acre or 3.63 tons/hectare) [6].
Yield is a complex inherited character resulted from the interaction between the vital processes [7] and associated with various contributing characters, therefore, direct selection for yield per se may not be the most efficient method for its improvement, but indirect selection for other yield related characters, which are closely associated with yield and high heritability estimates will be more effective [8]. In the year 2017/2018, the United States was the largest producer of corn with a production volume amounting to about 370.96 million metric tons. China and Brazil rounded off the top corn producing countries. Total maize production in Maize output increased the most, reaching a record in 2017, driven largely by higher production in several major exporting countries. Wheat output was  (11 Mt) and Argentina (10 Mt). Increased production in Brazil will be largely driven by higher second-crop maize following soybeans. Production growth in the United States is expected to slow to less than 1% p.a. over the next ten years, compared to 2.4% p.a. the decade before, due to slower growth in domestic demand, particularly for ethanol, and increased export competition [9].
A successful selection program depends not only on heritability of desirable characters but also on the information about the association among various yield component characters and their association with grain yield [10] [11]. Variability is the differences between individuals in a population due to genetic composition and growing environment [12]. The existence of variability is utmost role as success of any plant breeding program depends on the genetic variability and selection skill of plant breeder [13]. Selection is only effective if the parent population have significant amount of variability. Phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) provides insight information on magnitude of variability in a population and heritability shows the component of a character transmitted to future generations [14] [15].
Furthermore, heritability coupled with genetic advance shows the expected genetic gain in next generation [16]. In addition, correlation between yield and yield attributing traits are prime important for indirect selection for those traits which have high heritability and closely associated to contributes to improve yield [17]. This study was conducted to elaborately understand about the genetic parameters and character associated attributes to consider the yield and its components involving 35 Maize populations to achieve following objectives: to know the yield potentiality of different maize genotypes, to understand the nature of association of traits, direct and indirect relation between yield contributing characters of maize genotypes and to study genetic variability among the genotypes.

Materials and Methods
The research work was conducted in the experimental field of Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, during Rabi season (Mid December to May 2018). Sher-e-Bangla Nagar Thana (Dhaka metropolitan) area 5.25 sq km, located in between 23˚45' and 23˚47' north latitudes and in between 90˚22' and 90˚23' east longitudes. The soils of this AEZ have been developed from the Madhupur Clay and occupy a large area of 4244 km 2 [18].
Madhupur Tract or Red Soil Tract is a forest-based land. The area is also known for the production of mango, banana, pomelo and pineapple. But deforestation Advances in Bioscience and Biotechnology in the last few decades has made a great loss to the environment which will not be recovered in future [19]. The optimum soil pH is 6.6 -7.3 [20]. 35 Maize genotypes were used to execute the experiment in 360msq of land in three replications during 2017-2018 (Rabi season) cropping season. The germination percentage of the genotypes was satisfactory, uniformed with 94% -96% respectively. The experiment was designed and laid out in Randomized Complete Block Design (RCBD) with 3 replications. The supplied experimental land was 360 msq in total. The field was divided into 3 blocks and the individual block size was 3 m × 30 m. Spacing was maintained 60 cm × 20 cm, row to row distance was 60 cm, line to line distance was 20 cm, block to block distance was 1 m. The Genotypes were distributed randomly without biasness. Green manure and decomposed organic matter were used at the rate of 6 ton/ha before final land preparation.
For land preparation chemical fertilizers were applied such as Urea (14 kg

Genetic Diversity
Genetic diversity serves as a way for populations to adapt to changing environments. Thus, genetic diversity plays in important role in improvement of new breeding program through selection and introduction with diversified crop characters. The capability of the population to adapt to the changing environment will depend on the presence of the necessary genetic diversity [22]. The success of hybridization depends upon the selection of suitable parental genotypes and performance of their cross combinations.

Non-Hierarchical Clustering
In this non-hierarchical clustering, 35 maize genotypes were used and grouped  Table 1.

Principal Component Analysis (PCA)
Here, principal component axes were estimated according to the Eigen values, percent variation and cumulative percent variation. The results showed the first principal axis for days to male flowering greatly accounted for the variation among the genotypes representing 31.51% of the total variation among the genotypes ( Table 2).
The first seven characters of the principal component axes with eigen values was accumulated around 96.70% of the total variation among the eleven characters. The rest four characters were contributed around 3.3% of the total variation (Table 3). According to the principal component analysis, the principal component axis showed two-dimensional scatter diagram using 35 maize genotypes.
The scatter diagram is represented in Figure 1.

Inter and Intra Cluster Distance
Through canonical variate analysis inter and intra cluster distance was analyzed and presented in Table 4 and nearest and farthest cluster distance are presented in Table 5. The maximum value for the cluster distance D2 were recorded in  (Table 5).
Here, the intra cluster distance was observed in cluster I (1.23), II (0.00), III (0.76), IV (2.08) and V (1.89) respectively. The highest intra cluster was recorded in cluster IV (2.08) and lowest in cluster III (0.76) that showed that the genotypes within the intra cluster distances were closely related and inter cluster distances were recorded higher and larger than intra cluster distances that indicated the genotypes had wider genetic diversity among the genotypes in different groups (Table 4 and Table 5).

Cluster Diagram
The cluster diagram showed the distribution of the 35 maize genotypes grouped into five sections. The positioning nature of the genotypes were remarkably diversified among the genotypes (Figure 2).

Cluster Mean Analysis
The cluster mean analysis was estimated using 35 maize genotypes grouped into five cluster with eleven characters involvement (Table 3). Here, within the clusters compared the considerable differences among the clusters for all the character studied. The maximum cluster mean value for days to male flowering was observed in cluster III (65.5) and the minimum was in cluster II (62.0). In case of days to female flowering, the maximum mean value was observed in cluster II

Contribution of Characters towards Divergence of the Genotypes
Relative contribution of characters towards divergence of the genotypes was observed and presented in Table 6. The character involved in high absolute magnitude for vector 1; considered to be responsible for primary differentiation and the character involved in high absolute magnitude in vector 2 was considered to be responsible for secondary differentiation. If the same character was obtained equal magnitude for the both vectors than the character was considered responsible for primary at the same time secondary differentiation.   thus, these two characters are important towards the divergence of the genotypes investigated (Table 6).  (12; G4, G5, G11, G13, G14, G18, G21, G27, G29, G31, G34, G35) and the lowest were 3% genotypes were included in cluster number II (1; Pacific G12). Between inter and intra cluster mean values, inter cluster distances were recorded higher than the intra cluster distances which indicated wider genetic diversity among the genotypes of different groups involved. The maximum value for the cluster distance D2 was recorded in cluster III (18.740) followed by cluster II (15.470, 13.032). The farthest cluster distance was recorded in cluster III that means it represented the highest diversified genotypes than other clusters.