Risk Factors for Viral Non-Suppression among People Living with HIV and Major Depressive Disorder in Uganda

Background: Several studies indicate that depression is associated with nonviral suppression among persons living with HIV (PLWH) using antiretroviral therapy (ART) worldwide. However, among PLWH with major depressive disorder, factors associated with non-viral suppression remain uncertain. We determined the prevalence and identified the factors associated with viral non-suppression among PLWH with major depressive disorder using ART in Northern Uganda. Method: A total of 30 primary care HIV clinics across three northern districts (Gulu, Kitgum, Pader) participated in the study. Using baseline data from the SEEK-GSP study, a cluster-randomized trial in northern Uganda (2016-2019) that involved 1140 PLWH with mild to moderate major depressive disorder; we examined the demographic, clinical, and psychosocial factors using standardized questionnaires. Data on viral load was abstracted from clinic records and dichotomized into suppressed (<1000 How to cite this paper: Bulage, L., Akimana, B., Namuli, J.D., Musisi, S., Birungi, J., Etukoit, M., Mojtabai, R., Nachega, J.B., Mills, E.J. and Nakimuli-Mpungu, E. (2022) Risk Factors for Viral Non-Suppression among People Living with HIV and Major Depressive Disorder in Uganda. World Journal of AIDS, 12, 43-54. https://doi.org/10.4236/wja.2022.122004 Received: September 21, 2021 Accepted: April 8, 2022 Published: April 11, 2022


Introduction
The number of persons living with HIV (PLWH) accessing anti-retroviral therapy has rapidly increased over time in Sub-Saharan Africa. [1]. The revision of the ART eligibility criteria in 2015 by the World Health Organization (WHO) recommends treatment initiation for all the HIV-infected, regardless of their immunological status (dubbed "universal test and treat"). Furthermore, it facilitated the increase in the number of persons accessing ART [2], In Uganda, the number of PLWH accessing ART increased by more than 100,000 annually, from 313,117 in 2011 to 570,486 by late 2013 then 1,140,550 in 2018 [3]. Uganda has anestimated 1.3 million persons living with HIV (PLWH) translating to a 6.2% prevalence of the general population.
The rising numbers of patients accessing ART call for the sustainability of the treatment success and limits treatment development failure through monitoring patients once on treatment and this allows timely detection of treatment failures. WHO in July 2013 recommended viral load testing usage as the gold standard to monitor patients' responses to ART [4]. Since the introduction of universal viral load testing in Africa, several countries including Uganda have embraced the recommendation.
towards the elimination of HIV that included a 90% diagnosis of HIV infected individuals with access for treatment to the same and with a 90% viral suppression to the treatment initiates [6].
Several studies have documented a strong association between depression and poor HIV treatment outcomes, particularly, ART adherence and viral non-suppression [7] [8]. Systematic reviews of published studies on prevalence of depression among ART users in Sub-Saharan Africa report estimates of 20% -30% and indicate that depression is associated with poor ART adherence [9] [10].
However, studies of PLWH with depression are limited worldwide. In this sub-group of PLWH, we are uncertain of those factors that are associated with poor HIV treatment outcomes. In this paper, we determine the prevalence and factors associated with viral non-suppression among PLWH with major depressive disorder and on ART in northern Uganda.  [11], antidepressant naïve, and using ART. We excluded those with psychotic symptoms, high suicide risk, severe medical disorders such as pneumonia or active tuberculosis, hearing, and visual impairment.

Study
The HIV clinics involved in the study were situated in three post-conflict northern districts (Gulu, Pader and Kitgum). Most clinics initiated their ART programs in 2005 and provide ART care free of charge. Viral load monitoring was introduced in the region in August 2014.

Recruitment Process
We used study teams that reflected the ethnicity of the target community at each of the participating primary health centers. The study teams worked with the trained lay health workers (LHWs), who are the first level of health care delivery in the country, to spread information about the study by word of mouth in villages within the study region. The study team conducted presentations in the community to explain study purpose and procedures to facilitate community understanding of the trial activities.

Data Collection
Trained research assistants worked with HIV care providers at each participating clinic to give health talks on depression to clients in the waiting area.
Clients who felt that they had experienced depression symptoms as per the health talk were then invited for further evaluations using the Luo version of the 20-item self-reporting questionnaire [12] and the MINI depression module. Clients explained study procedures, determined eligibility, and then obtained informed consent. Each client who gave informed consent received baseline assessments with a standardized questionnaire.

Study Measures
Socio-demographic variables were assessed using a demographic questionnaire that asked about descriptive information including age, gender, number of children, education, and relationship and employment status. Employment status was categorized into ''unemployed", "employed", and "peasant farmer". Relationship status was categorized into ''never married," "married/living with a partner", "divorced/separated", or "widowed". Education status was categorized into "primary/no formal education" and "secondary and above".
Depression symptoms were assessed using the SRQ-20 [12]. Cross-cultural adaptation and validation of the SRQ-20 in PLWH in southern Uganda showed that an optimum cut-off point of six or higher had a sensitivity of 84% and a specificity of 93% for current depression. In this study sample, SRQ scores were modeled as a continuous variable with a Cronbach alpha reliability coefficient of The SAD PERSONS scale was used to assess the suicide risk [13]. The total score ranges from 0 to 10. Scores of <4 indicate low risk); scores of 5 -7 indicate moderate risk; and scores of 8 -10 indicate high risk. Post-traumatic stress symptoms were assessed using the locally adapted Harvard Trauma Questionnaire (HTQ) [14]. The total score ranges from 16 to 64 and a total score ≥ 36 is indicative of post-traumatic stress disorder.
Alcohol use was assessed using the 10-item alcohol use disorders identification test (AUDIT) [15]. The total score ranges from 0 to 40 and a score of ≥8 is indicative of hazardous use. AUDIT scores were modelled as a continuous variable. In this study population, the measure attained a Cronbach α of 0.95.
We used the modified coping inventory to assess a broad range of both positive and negative coping responses which establish how the study participants responded when they were confronted with difficult or stressful events in their lives [16]. Each coping strategy is assessed by a set of two questions. Responses were based on a four-point scale. For each coping strategy, the scores range from two to eight, with higher scores indicating frequent use of the coping strategy. A binary variable was created whereby frequent use of 1 or more coping skills was coded 1 while non-use was coded 0.
To measure internalized stigma, we used the brief AIDS-related stigma scale [17]. Responses were based on a four-point Likert scale. The scores ranged from 8 to 32 with high scores indicating higher levels of internalized stigma.
Adherence to antiretroviral therapy was assessed using one question "During the past week, how many days have you missed taking all your medication doses?" Adherence was calculated as the percent of days in the week the person missed all medications. Adherence "scores" were treated as a dichotomous vari-

Discussion
We found the prevalence of viral non-suppression at 12% among PLWH with major depressive disorder and on ART. The prevalence odds of viral non-suppression were higher among PLWH with moderate to high suicide risk and those who had used ART for a long duration of ≥4 years.
Although depression in PLWH has been associated with a lower likelihood of adherence to ART probably leading to viral non-suppression, our study revealed a relatively low viral non-suppression prevalence which is also similar to the national prevalence of 13% among the general population of PLWH as indicated in the Ugandan study based on routinely generated viral load monitoring program data from the entire country [18].
Factors that increase the suicide risk include male gender, young age (15 -24  [19]. Accumulation of these factors raises the risk of suicide. None of these factors bythemselves was associated with viral suppression in our study. But, when combined in a suicide risk score [13], we found that the higher the suicide risk score the higher the prevalence odds for non-viral suppression.
This finding is in keeping with prior research in high income countries. López et al. studied 648 PLWH and found that individuals who endorsed suicidality were more likely to have unsuppressed viral loads [20]. Similarly, Durham et al.
conducted a cross-sectional survey of 6706 PLWH in the United States and found that increased risk for suicide was associated with unsuppressed viral load [21]. Also, Quinlivan et al. conducted a cross-sectional analysis among 4099 PLWH in the United Statesand foundan association between moderate to high suicide risk and unsuppressed viral loads [22]. To our knowledge, this study is the first to demonstrate the association between suicide risk and unsuppressed viral load in Sub-Saharan Africa.
An accumulation of suicide risk factors indicates severer depressive disorder [22]. Given that there is no mental healthcare in HIV treatment centers in northern Uganda, it's possible that depression progressed to severer states over time. This and other factors such as anti-retroviral drug resistance may explain why study participants with a longer duration on ART were also more likely to have non-viral suppression. This finding is not unique to our study.
Some studies of viral suppression in other HIV populations have also reported an association between long duration of ART and non-viral suppression [23] [24]. In Uganda, a study based on routinely generated viral load monitoring program data from the entire country reported a similar association [17]. On the contrary, a study conducted in South Africa found that a shorter duration on ART was associated with viral non-suppression [25]. An elevated viral load may indicate either poor adherence or drug resistance [26] [27]. In our study sample, adherence rates were quite high at 78% and ART adherence was not associated with viral non-suppression. Severe depression symptoms or drug resistance may be playing a role in viral non-suppression.
In our study, it is possible that our patients were experiencing treatment resistance, given that they had been on ART for a long duration. This finding calls

Clinical implications
Our findings call for critical need for investment into mental health care services in Uganda with more focus on the post-conflict districts of Northern Uganda to ensure routine assessment of depression and suicide risk among PLWH. Increased prevalence odds of viral non-suppression among chronic ART users with major depressive disorder requires additional investment into the viral load testing program to allow routine assessment for treatment resistance.

Limitations
We assess viral non-suppression based on routinely generated HIV/AIDS care program data at health care facilities. Although we were able to access the patients' medical charts to obtain viral load testing results, the charts did not indicate the specific assay used to derive the results. According to the Uganda MOH viral load monitoring guidelines, viral non-suppression is defined as having ≥1000 copies of viral RNA/ml of blood for plasma. However, studies have shown that this is a conservative threshold to define viral non-suppression that can lead to an underestimation of those who experiencing ART failure [31] [32].

Conclusion
Suicide risk and long-term ART usage are associated with viral non-suppression among anti-retroviral therapy users with mild to moderate major depressive disorder in Uganda. As ART is scaled up across Sub-Saharan Africa, greater attention must be paid to integrating first-line psychological interventions for depression treatment into routine HIV care.