Outbreak, Distribution and Damage Characteristics of Cocoa Stem Borer, Eulophonotus myrmeleon Felder 1874 (Lepidoptera: Cossidae) in Major Cocoa Producing States in Nigeria

This study aims to ascertain the extent of the cocoa stem borer Eulophonotus myrmeleon distribution, outbreak, and damage to the cocoa plantations in six cocoa growing states in Nigeria. Three local government areas were selected from each of the six major cocoa states and three farms from each local government area. In each farm, 100 cocoa trees were randomly selected and in-spected for symptoms and damages of E. myrmeleon. The result revealed that E. myrmeleon infestation has increased significantly (p = 0.01, df = 1, F = 41.77) in 2019 by 10% from the 2014 inspection. It also showed that the infestation level was significantly different from state to state. Symptoms of infestation include presence of entry/exit holes, woody exudates around the trees, yellowing and browning of the leaves. Damages caused by E. myrmeleon infestation are, loses of pod on the affected branch and tree and death of tree. Field observations showed clearly that stem borer is now becoming a serious problem and limiting considerably the production of cocoa in the region. It is time to give more attention to the biology and management of the pest. This is the first report detailing the symptoms and damage done by cocoa stem borers in Nigeria.


Introduction
The cocoa crop is afflicted by a wide range of pests, weeds and diseases which can cause very substantial losses, impacting on the livelihoods of farmers and the economies of producing countries [1]. Insect pests have largely contributed to the declining productivity of cocoa in Nigeria. For example, about 25% -30% loss in yield of cocoa has been attributed to the cocoa mirid, Sahlbergella singularis while about 17% is lost through the feeding of the cocoa pod borer Characoma strictigrapta [2]. Some cases of major outbreaks could result in total damage (95%) of the plantations. For instance, the pest outbreak in parts of Ondo and Edo states reported in May 2012 caused a complete damage of the plantations [3].
Eulophonotus myrmeleon Felder (Lepidoptera: Cossidae), is a moth in the family Cossidae. The menace of cocoa stem borer, E. myrmeleon has earlier been recorded in Cameroon, Côte d'Ivoire, Congo Republic, Ghana, Nigeria, Sierra Leone, Togo and the island of Sao Thomé, Democratic Republic of Congo and South Africa where it attacks members of the Sterculiacae family [4] [5] [6] [7].
Apart from Theobroma cacao, the larvae of E. myrmeleon feeds on Cola acuminate, Cola nitida, Triplochiton scleroxylon, Acalypha spp, Populus spp, Coffea spp and Combretum spp. [6] [8]. However, they were considered a minor pest of cocoa as their populations occurred in low numbers and the level of attacks was usually very low, with less than 5% of the trees showing damage. Due to the above occurrence of the pest, very little work was conducted to address the problem [9].
Recently, outbreaks of E. myrmeleon has been reported to cause damage to cocoa trees in the West and Central African producing countries, including Côte d'Ivoire, Ghana, Cameroon, Nigeria and Togo [10] [11] [12] [13] [14]. However, since 2018, it has been observed that damage caused by cocoa stem borer, E. myrmeleon Fldr. (Lepidoptera: Cossidae) in Nigeria is becoming increasingly important.
As a follow up to serious outcry by farmers a survey was carried out in selected cocoa plantations within Nigeria to ascertain the extent of the recent outbreak, their distribution and extent of damage to the cocoa plantations by the stem borer, E. myrmeleon.

Methodology
The study on the distribution of cocoa stem borer was in six cocoa growing states in Nigeria A three-stage purposeful sampling technique was used to identify subjects for the survey.  In each local government area, there was a general interaction with small farmers groups to first ascertain their knowledge perception of the pest, after which three farmer with good knowledge volunteered and took us to their infested farms. In each farm, a total of 100 randomly selected cocoa tree stands were sampled by systematically traversing the farms at both diagonals and longitudinal ends. Each selected cocoa stand in each location was closely observed for the presence or absence of the E. myrmeleon symptoms on their various parts (trunks, stems, pods, cherelles, twigs, leaves), which was appropriately recorded.
The activities of cocoa stem borer were randomly observed in its natural habitat on all the selected cocoa plots. Each infested cocoa stand was closely observed for symptoms on the various parts (trunks, stems, pods, cherelles, twigs, leaves) of the cocoa trees. They were further cut open to show the tunneling patterns of the borer. Field pictures were taken with a digital camera and detailed descriptions of the tunneling pattern of the borers were made.
Random destructive sampling of infested cocoa stands was carried out by cutting off some stems and trunks with sharp cutlass. They were further cut open to expose the various developmental stages of the borer. Field pictures were taken with a digital camera and detailed descriptions of the various stages of the borers Advances in Entomology observed were made.
All data generated from this study were analyzed using simple descriptive statistics of their various means and multiple factors analysis of variance (ANOVA) in Minitab with subsequent mean separation done using Tukey's test.

Survey of Cocoa Stem Borer and Pest Incidence Mapping
There

Damage Characteristics of Cocoa Stem Borer
The cocoa stem borer E. myrmeleon is a serious insect pest of Theobroma cacao, which sometimes kills the tree completely within a few months of infestation.
The initiation of attack could be recognized by the presence of small holes on the cocoa tree trunk/branches followed by gummosis, extrusion of chewed up fibers and excreta at the base of trunks as the grub expels them out. These holes increase as infestation progresses (Figures 4(a)-(c)).
The larva of E. myrmeleon bores and tunnels into the trunks and stem branches of cocoa, enlarging the gallery as it grows. The larvae spend their entire immature life inside the cocoa trunks and stem branches. The damage caused by the cocoa stem borer E. myrmeleon is characterized by the presence of wood shavings and frass deposits at the entrance of the hole in the trunk/branch ( Figure 5(a)) and at the base of the cocoa tree ( Figure 5(b)). In addition, emissions of gummy mucilage exudates were noticed flowing off from the entrance hole down the trunk of the tree (Figure 5(c)).
Their feeding activities damage the vascular tissues, arrest the sap flow, and weaken the stem thereby resulting in yellowing, browning and shedding of leaves, drying of twigs, which sometimes results in the death of the tree. If a branch is affected, you could see the leaves on that branch drying up while the rest of the leaves are still fresh. As the infestation progresses, it can spread to all parts of the tree, killing it. Even at the peak of the rainy season, the infested cocoa plantations from a distance will appear like it has been ravaged by fire ( Figure 6).      (Figure 6). Majority of the tunneling was upward towards the branches however, sometimes, their tunneling is downwards towards the underground roots (Figure 7). It was also observed that young cocoa trees less than one year old were not attacked.

Description of the Various Stages of the Cocoa Stem Borer
The eggs of the cocoa stem borer were conspicuously absent at the time of this field assessment as they may have all hatched and progressed to other life stages   The adult borer was totally evasive throughout the period of this field survey.
The difficulty encountered in rearing the larvae of the cocoa stem borer and thereby getting the adult insect for proper identification and description had remained a big challenge for us in Nigeria. The larvae remain in the stem of the cocoa and pupate ( Figure 9) and then emerge as adults.

Discussion
The cocoa stem borer, Eulophonotus myrmeleon, which hitherto before now was regarded as a minor pest with minimal damages inflicted on cocoa trees [7] [14], is fast assuming a major pest status across all the cocoa growing states in Nigeria especially in Cross River State. The E. myrmeleon Felder has now become a serious cocoa insect pest in Nigeria with serious economic consequences.
The result of this study shows that the level of attack of E. myrmeleon increased astronomically from 2015 to 2020, indicating that this insect has become a serious problem for the farmers in the states sampled. The current pest incidence in 2019/2020 was generally high across cocoa agro ecologies of the six (6) cocoa producing states covered by the survey. This damage proportion is considered economical for tree crops like cocoa especially because of the nature of damage inflicted on the plant, recovery of the stem is rarely possible. This corroborates the report by [15] who reported that E. myrmeleon which was not considered a serious pest of cocoa in Cote d'Ivoire is now a serious menace with above 25% infestation. [16] reported a rise in rainfall and lower temperature to play an important role in the rise of the population of E. myrmeleon in Cote d'Ivoire.
The emerging larvae desert galleries in drying cocoa trunk and stem branches wood, to make excavation of new galleries in fresh wood beneath the protection of a tent of wood particles bound together with silk. This usually results in a single cocoa tree being riddled with 5 to 30 fresh bores or more at the same time.
The borer is a slow spreading pest as it has a long-life cycle. They were found to aggregate in clusters of cocoa trees. The pest appears to gradually spread to neighboring trees, which results in farms having areas with stem borer attack, while other areas appear untouched [17].
There was an earlier belief that the cocoa stem borer spreads fast as a pest in areas with heavy pesticide abuse on trees, which kills off their natural predators [8]. This may be a possible explanation to the spread of the pest in cocoa farms. there are no known effective methods for the management of the pest. The use of pesticides for the management of E. myrmeleon in cocoa field is challenging as most of the life stage of the pest is spent hidden in the stem or branches of the tree therefore pesticides used in managing other pest like the capsids are ineffective against E. myrmeleon [14]. Management strategy for E. myrmeleon need to be studied and developed urgently to reduce the spread and the limit the damages caused by E. myrmeleon to cocoa production.

Conclusion
Very high damage symptoms of this Lepidopterous insect pest were observed in farmers farms. Indeed, the cocoa stem borer, which had been reported in Nigeria, before now as a minor insect pest, is fast becoming a major pest of cocoa and urgency must be given to its study to fully understand its biology and develop a sound management measure for this pest. Meanwhile, the farmers are advised to adhere strictly to the guidelines of Good Agricultural Practices in their cocoa farm to stem down the current high incidence of the pest. The biology of the Eulophonotus myrmeleon should be studied in detail and methods for the control of this pest are researched. Movement of plant material should be done carefully to avoid the movement of this pest from areas of high densities to areas of low densities.