Magnesium Sulfate Effect on Fetal Umbilical Artery and Middle Cerebral Artery Doppler Indicies in Women with Severe Preeclampsia and Eclampsia

Objective: To study MgSO 4 effect on fetal MCA and UA blood flow changes using Doppler ultrasound in cases of severe PET and eclampsia. Materials and Methods: A total of 40 patients with severe PET admitted to El-Shatby Maternity University Hospital, Alexandria (Egypt) were examined before and after administration of MgSO 4 using Doppler study to measure fetal MCA and UA blood flow changes. Results: After administration of MgSO 4 , the mean RI of UA, PI of UA showed a statistically significant decrease (P < 0.001) also the systolic-diastolic ratio (p = 0.001). Mean resistivity index (RI) cerebral showed a statistically significant increasing (P = 0.001), pulsatility index (PI)-cerebral and the systolic-diastolic ratio showed a statistically significant increasing (P < 0.001). The C/U (cerebroumblical) ratio increased after the treatment (P < 0.001). Conclusions: Infusion of MgSO 4 significantly decreases the fetal RI, PI, SDR umbilical and increases the fetal RI, PI, SDR MCA and increases cerebroumblical ratio indices obtained by Doppler examinations. To study effect on and UA blood changes using in of

PET and eclampsia are among the most important diseases of pregnancy causing maternal morbidity and mortality associated with high vascular resistance due to vascular spasm with impaired placental blood flow [2].
MgSO 4 is the best drug used in treatment of severe PET and eclampsia and prevent maternal and fetal complications [10] [11]. Effect of MgSO 4 on the brain vascular blood flow is still unclear as it may reduce the vasospasm [12] [13] [14] [15].
The MgSO 4 changes vascular permeability and reduces the edema [10]. It seems to have no adverse effects on the fetus [16], but in response to MgSO 4 injection, fetal brain perfusion may be also altered [17].
Doppler ultrasound is the best mood to study the mechanisms by which PET  [28]. MgSO 4 effect on the fetal adaptive changes in PET is one of the most important research issues [29].
Aim of the Work: To study MgSO 4 effect on fetal MCA and UA blood flow changes using Doppler ultrasound in cases of severe PET and eclampsia.

Material and Methods
In this study, all the participants were evaluated before and after MgSO 4 administration. This study was approved by the ethics committee of the Faculty of Medicine University of Alexandria. Pregnant cases with sever PET and eclamptic fits who attended to El-Shatby Maternity University Hospital during the period between December 2019 and December 2020 were involved in the study. Inclusion criteria were: Age in the range of 18 -40 years, Singleton pregnancy, Any gravidity or parity, Gestational age equal to or above 28 weeks, Systolic blood pressure ≥ 160 mmHg or diastolic pressure ≥ 110 mmHg on two occasion six hours or more apart on bed rest, Preeclampsia with new onset grand seizures [30], Spontaneous pregnancy and One or more of the following [30] [31] [32] [33]: (Proteinuria of 24 hour urine of at least 5 g of protein or 3+ or more on two random samples collected four or more hours apart, Sudden oliguria. Central nervous system disturbances, Pul-monary edema or cyanosis, Epigastric or right upper quadrant pain, Liver dysfunction (increase serum levels of SGOT and SGPT), Thrombocytopenia < 100,000/cmm, Fetal growth restriction calculated by Hadlock formula [34]. Exclusion Criteria were: Cases with other systemic disease (e.g., diabetes, hypertension, and autoimmune disorders), multiple pregnancy, Fetuses with congenital anomaly, History of taking opium, smoking and twin pregnancy.
The goal of the study and the method were described to all patients and written consent form was taken prior to the initiation of study.
After admission of the patient, the following was done: 1) Complete history taking.
2) General and local examination.
3) Color Doppler ultrasound of fetal UA and MCA, the cerebro-umbilical ratio (middle cerebral artery resistance index/umbilical artery resistance index), pulsatility index (PI) and S/D ratio will were evaluated. Then again MCA and UA Doppler evaluation was done to evaluate blood flow changes.

Outcome
This result was compared with APGAR Score of the baby and the out-come of the mother.

1) Umbilical artery Doppler indices
There were statistical significant differences between umbilical artery PI, RI and cerebroplacental ratio with administration of MgSO 4 (p < 0.001); as shown in Table 1.

2) Middle cerebral artery [MCA] Doppler indices
There were statistical significant differences between MCA them (p < 0.001); as shown in Table 2.
3) Relation between ultrasonographic assessment of amount of liquor and cerebro-placental ratio (C/U) before and after mgSO 4

administration
The Cerebro-placental ratio(C/U ratio) before and after mgSO 4 has statistical significant differences in both groups (p = 0.009) and (p = 0.004) with no statistical significant differences between both of them (p = 0.211); as shown in Table   3.

4)
Relation between estimated fetal weight (gm) and cerebro-placental ratio (C/U) before and after mgSO 4 administration There were 28 cases of normal fetal biometry and 12 case of intrauterine growth restriction. As shown in Table 4.
The Cerebro-placental ratio (C/U ratio) before and after mgSO 4 has statistical N. M. Hefil Open Journal of Obstetrics and Gynecology  significant differences in both groups (p = 0.013) and (p = 0.008) with no statistical significant differences between both of them (p = 0.250). As shown in Table  4.

Maternal outcome
There were no cases of maternal deaths and all cases recovered well. Open Journal of Obstetrics and Gynecology Table 3. Relation between ultrasonographic assessment of amount of liquor and cerebro-placental ratio (C/U) before and after MgSO4 administration.

Discussion
Preeclampsia is one of the most important serious diseases of pregnancy and Open Journal of Obstetrics and Gynecology occurs in 5% to 7% of pregnant women [34]. Doppler mood ultrasonic evaluation is used to evaluate blood flow in fetal vessels [35].
A low index of pulsatility in the MCA associated with fetal decompansation has been described [36].
Because the cerebroumblical ratio is providing information about placental perfusion and about fetal changes so its an important factor in estimating fetal outcome and well being [37].
The objective of this study was to assess the effects of administration of MgSO 4 on Doppler parameters of middle cerebral and umbilical arteries in cases of severe PET and eclampsia.
The study was conducted on 40 pregnant female selected from EL Shatby Maternity University Hospital during the period between December 2015 and December 2016.
There were 28 cases with normal fetal growth and 12 cases with intrauterine fetal growth restriction. There was statistically significant improve in the C/U ratio after MgSO 4 administration in both of them.
Regarding the Doppler indices studied in our study, the injection of MgSO 4 could significantly decrease the RI, PI and SD ratio of the umbilical artery, increase the RI, PI and SDR-MCA, thus increase the C/U ratio.
They agree with our study as regard the umbilical artery only but against it as regard the MCA.
In addition, Souza et al., [38] [39] showed the reduction of PI in the fetal umbilical artery and MCA due to the injection of MgSO 4 .
Qiao, et al., [40] have found significant changes in parameters after administration of MgSO 4 . In our study, we found significant improvement of C/U ratio after using MgSO 4 treatment with improvement of fetal circulation as it cause umbilical artery vasodilation [41].
Coleman et al. [42] have found a significant relationship between the resistance index and pregnancy outcomes in 114 pregnant women at 24 -22 weeks who underwent Doppler sonography. Results of another study have showed a significant relationship between the vascular index and pregnancy outcomes, including fetal growth restriction [43], but results of our study were not in concordance with the last two studies. This difference could be due to the time of Doppler sonography in our study. There was also a control group in order to compare the two groups (treatment and control groups) in terms of resistance index and pregnancy outcomes in these studies, which could produce impressive different results.

Conclusions
• Infusion of MgSO 4 significantly decreases the fetal RI, PI, SDR umbilical and increases the fetal RI, PI, SDR MCA and increases cerebroumblical ratio in-