Carbon Sequestration Potential of Tree Species at Isabela State University Wildlife Sanctuary (ISUWS), Cabagan, Isabela, Philippines

This study is conducted to assess the amount of carbon stored in the aboveground biomass of the tree species at the Isabela State University Wildlife Sanctuary (ISUWS). A total of 34 different tree species were found with 285 individuals were identified with a total of 47.50 t/ha Carbon stock and 164.09 t/ha of accumulated CO 2 . It was found in the study that Alstonia scholaris contains the largest amount of above-ground biomass (AGB) with a mass of 20.97 t/ha and Carbon stock of 9.44 t/ha followed by Samanea saman with a mass of 13.40 t/ha and Carbon stock of 6.03 t/ha. Based on the result and conclusion of this investigation, the following recommendations were drawn: Conduct a study concerning the carbon emission of the area to determine the relationship with its carbon sequestration potential; and conduct tree planting activity to open areas in the study site to increase its carbon stock potential and fully serve the purpose of the area as a wildlife sanctuary.


Introduction
Global warming is among the most dreaded problems of this millennium which just represents one aspect of climate change. This is caused mostly by increasing concentrations of greenhouse gases in the atmosphere resulting in changes in climate pattern. Greenhouse gases (GHGs) which are primarily carbon dioxide, methane, nitrous oxide and others have influenced the earth's climate system [1]. Among the GHGs, carbon dioxide (CO 2 ) is the most important and abundant gas in the atmosphere and is responsible for more than half of radiative forces along with the greenhouse effect [2]. Forest ecosystem plays a very important role in the global carbon cycle by sequestering a substantial amount of CO 2 from the atmosphere [3]. Trees take up CO 2 from the atmosphere and store carbon in their biomass (roots, stems, and foliage) through the process of photosynthesis [4]. In 1990 alone, it is estimated that the world tropical forests were a net source of carbon (1.6 billion t) due to anthropogenic activities including land-use changes and forestry activities, primarily tropical deforestation [5]. The Philippine forests in particular, through massive deforestation, were found to have contributed about 3045 tons of Carbon to the atmosphere since the year 1500 up to the present era [2].
The Kyoto protocol provides flexible mechanisms, where Clean Development Mechanism (CDM) is most relevant for developing countries such as the Philippines [6] which have potential for carbon trading. Due to the importance of forest contributions to global climate change, research on Carbon budgets in forest ecosystem has been intensive in the Philippines. However, there are still significant gaps in the current understanding of the carbon sequestration potential of different plantation species in the country [2]. Hence, this study intends to assess the amount of carbon stored in the above-ground biomass of the tree species at the Isabela State University Wildlife Sanctuary (ISUWS).

Method of Estimating Above-Ground Biomass
The mass of living organisms in a forest is called the biomass. Most of the biomass in a forest is in trees, and the focus of methods for estimating biomass is measuring the above-ground portion of trees [7]. It is the most important and visible carbon pool of the terrestrial forest ecosystem [8].
There are two approaches in measuring the above-ground biomass (AGB). The first one is the destructive method of tree biomass estimation and second is the non-destructive method. The former method is destructive and is not feasible for a large-scale analysis but experts generally agree that the harvest method is the most accurate and direct method in estimating the aboveground biomass and the carbon stocks stored in the forest ecosystems [3]. The latter method estimates the biomass of a tree without felling. This method of biomass estimation is applicable for those ecosystems with rare or protected tree species where harvesting of such species is not very practical or feasible [3].

Carbon Stock Estimation
Carbon stock is the term used for the carbon stored in terrestrial ecosystems, as living or dead plant biomass [9]. Estimation of the magnitude of sinks and sources of carbon requires reliable estimates of the biomass of forests and of individual trees [10]. As an overall estimate, the carbon content of biomass in the Philip- Open Journal of Ecology pines ranges from 41% to 49% with an average close to 45%. This is a little lower than the IPCC default value of 50% [11]. A study conducted on the carbon content of wood samples collected from secondary forests from several locations in the Philippines and reported that for Philippine biomass, a default value of 45% could be used in determining carbon stock in trees [12]. Some studies in carbon stock assessment in the Philippines use this amount of carbon like in the study in the Reservation Area of Kalinga State University which computed a total of 475.25 t/ha of carbon in [13]. Also the 45% carbon content of the tree biomass were used in the study in Kapatagan, Lanao Del Norte accumulated a total of 257.16 Mg/ha [14].

Materials
The materials used in the establishment of sampling quadrats are bolo, pegs and nylon rope. In the collection and preservation of leaf specimens, field note, presser, pruning shear, denatured alcohol, packing tape, specimen tag and sacks were used. After randomly choosing the coordinates of the base corner of sample plots, they were located with the used of Global Positioning System (GPS) and the base stake was placed on the ground.

Establishment of Quadrats and Transects
The 20 sample plots having the size of 20 m × 20 m were laid out within the study area ( Figure 1) using completely random sampling [7]. The approach in laying out the sample plots is by choosing random numbers from within the latitude and longitude of the study site. After randomly choosing the coordinates of the base corner of sample plots, they were located with the used of Global Positioning System (GPS) and the base stake was placed on the ground. It was located  in the southwest corner of the plots and oriented north-south as followed from Condit [7]. Within each sample plot, all trees having 10 cm and above in diameter at breast height (dbh) were recorded as followed from Brown (1997) [13].

Survey and Data Collection Procedure
The data gathered in this study was the dbh of every individual tree species. The dbh of every tree sample was measured at 1.3 m above the ground [15]. For forking trees below breast height, the dbh were taken and treated individually (plate 3). The dbh of a leaning tree was taken along its lower side, not the upper [7].

Data Analysis
The allometric equation developed by Banaticla was adopted to determine the above-ground biomass (AGB) of the tree species (Equation (1)) [10]. The carbon stock of the tree was determined by multiplying the computed AGB to 45% (Equation (2)) which is the average carbon content of wood samples collected from secondary forests from several locations in the Philippines [12]. The weight of CO2 sequestered was determined by multiplying the carbon stock of the tree to 3.67 (Equation (3)) which is the ratio of carbon to carbon dioxide [16].
where: CO 2 = carbon dioxide sequestered by tree species C = carbon stock of tree species 3.67 = ratio of CO 2 to C

Tree Species in ISUWS
The 20 sample plots established within the study area were covered with different vegetation such as grasses and patches of forests (natural and manmade).
The emphasis of this study is the tree covers where the tree species were identified and the number of individuals were counted. The 20 sample plots were covered with 34 tree species with an individual average number of 14.25 or a total of 285 individual trees. Table 1 shows the different tree species with their common name and their corresponding scientific name, number of individual trees and the plots where they were found. In the study it was found out that. ISUWS also homes some of Native endangered species.

AGB of the Tree Species in ISUWS
Biomass was used to provide an estimate of the carbon reservoirs in ecosystems based on the fact that about half of it is Carbon [13]. Biomass density (expressed as dry matter per unit area) indicates the potential amount of CO 2 that can be released to the atmosphere when vegetation is burned or cleared [8]. Table 2 shows the total above-ground biomass (AGB) of the selected tree species at ISUWS is 105.56 t/ha. The ABG of ISUWS is slightly lower than some of the secondary forest in the Philippines such in Cebu City with ABG of 195.13 t/ha [17] and Mindanao with ABG of 262 t/ha [14]. While in Kaliwa Watershed the computed AGB densities of secondary forests were 149.70 t/ha [18].
Among the 34 individual species, Alstonia schloris contains the largest amount of AGB with a mass of 20.97 t/ha followed by Samanea saman with the mass of 13.40 t/ha. The study of Udayakumar et al. [19] shows that Samanea saman contains 2.16 t/tree of AGB while the study of Marak et al. [20] shows that the AGB of Alstonia scholaris is 4.42 t/ha. The result of this study may be affected by their diameter as the both species belong to the highest diameter class. According to Guiabao [13], while the diameter of trees increases, the AGB also increases respectively.

Calculated Carbon Stock of Tree Species at ISUWS
In general, the total carbon stock of tree species at ISUWS is 47.50 t/ha ( Table   3). The carbon stock of the selected trees in the study is lower than the mean

Accumulated (CO2) of Tree Species in ISUWS
The total amount of accumulated CO 2 of the tree species at ISUWS is 164.09 t/ha (Table 4). A total of 88.17 kt was observed to be the CO 2 sequestration capacity of the forest stand in the reservation area of JRMSU-Tampilisan campus [16].
The variation in the amount of CO 2 sequestered and stored in the species within the forest stand was affected greatly by the stand density of trees of their total population and the area planted to these trees, aside from their biomass [16].

Conclusion and Recommendation
Based on the study conducted, the 285 individual trees found at ISUWS contain a total of 105.56 t/ha of AGB. The estimated carbon stock of the trees in the area is 47.50 t/ha with 164.09 t/ha of accumulated CO 2 . The researcher concluded that the tree species at ISUWS is lower than most of the secondary and urban forest in the Philippines but is still considerable to sequester high amount of carbon in the area. It was also concluded that the AGB was greatly affected by its diameter as this is the only data to estimate it. Based on the result and conclusion of this investigation, the following recommendations were drawn: Conduct a study concerning the carbon emission of the area to determine the relationship with its carbon sequestration potential and conduct tree planting activity to open areas in the study site to increase carbon stock potential and fully serve its purpose as a wildlife sanctuary.